Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,556,983 G→C 14.4% intergenic (+35/‑58) purU‑II → / → PP_5468 formyltetrahydrofolate hydrolase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,556,9830GC14.4% 157.9 / 16.6 62intergenic (+35/‑58)purU‑II/PP_5468formyltetrahydrofolate hydrolase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/26);  new base C (5/4);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGGTCCGCTTCGGCCGTGATGTGGAGAAGATGGTGCTGGCCCGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGCGAAGGCTGCCTGGCCCGCTACGACCCTGAAGCCCTG  >  NC_002947/1556837‑1557127
                                                                                                                                                  |                                                                                                                                                
cATGGTCCGCTTCGGCCGTTATGTGGAGAAGATGGTGCTGGCCCGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCtgctgc                                                                                                                                                 <  3:29973/148‑1 (MQ=255)
      ccGCTTCGGCCGTGATGTGGAGAAGATGGTGCTGGCCCGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGccc                                                                                                                                          >  5:110602/1‑149 (MQ=255)
       cGCTTCGGCCGTGATGTGGAGAAGATGGTGCTGGCCCGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCt                                                                                                                                         <  4:294925/149‑1 (MQ=255)
         cTTCGGCCGTGATGTGGAGAAGATGGTGCTGGCCCGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTg                                                                                                                                        >  1:189973/1‑148 (MQ=255)
             ggCCGTGATGTGGAGAAGATGGTGCTGGCCCGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCAc                                                                                                                                    <  2:248162/148‑1 (MQ=255)
              gCCGTGATGTGGAGAAGATGGTGCTGGCCCGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGa                                                                                                                                  <  7:275902/149‑1 (MQ=255)
               ccGTGATGTGGAGAAGATGGTGCTGGCCCGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGAc                                                                                                                                 <  1:50367/149‑1 (MQ=255)
                  tGATGTGGAGAAGATGGTGCTGGCCCGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAgg                                                                                                                              <  1:233379/149‑1 (MQ=255)
                        ggAGAAGATGGTGCTGGCCCGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCa                                                                                                                        <  2:31339/149‑1 (MQ=255)
                                tGGTGCTGGCCCGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTgcg                                                                                                                                                >  4:273422/1‑117 (MQ=255)
                                tGGTGCTGGCCCGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTgcg                                                                                                                                                <  3:273422/117‑1 (MQ=255)
                                 ggTGCTGGCCCGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCGCAGCAGcccgga                                                                                                                                       <  3:139575/125‑4 (MQ=255)
                                 ggTGCTGGCCCGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCGCAGCAGcccgga                                                                                                                                       <  7:335249/125‑4 (MQ=255)
                                 ggTGCTGGCCCGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCGCAGCAGcccgga                                                                                                                                       >  4:139575/1‑122 (MQ=255)
                                 ggTGCTGGCCCGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCGCAGCAGcccgga                                                                                                                                       >  8:335249/1‑122 (MQ=255)
                                    gCTGGCCCGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCgg                                                                                                             >  2:334207/1‑148 (MQ=255)
                                         cccGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCTACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATcc                                                                                                       <  6:110602/149‑1 (MQ=255)
                                          ccGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCt                                                                                                      <  3:246007/149‑1 (MQ=255)
                                          ccGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCt                                                                                                      >  6:58998/1‑149 (MQ=255)
                                             tGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTggg                                                                                                   <  3:22614/149‑1 (MQ=255)
                                                ccTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAgc                                                                                                                    <  7:342532/129‑1 (MQ=255)
                                                ccTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAgc                                                                                                                    >  8:342532/1‑129 (MQ=255)
                                                  tGCGTTATCACCAGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGaaa                                                                                              >  6:174001/1‑149 (MQ=255)
                                                    cGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGaaa                                                                                              <  5:174001/147‑1 (MQ=255)
                                                     gTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACgg                                                                                           <  4:305098/149‑1 (MQ=255)
                                                        aTCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGTTTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCaa                                                                                        >  1:112825/1‑149 (MQ=255)
                                                         tCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAAt                                                                                       <  2:189973/149‑1 (MQ=255)
                                                            ccTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGAc                                                                                    >  7:36009/1‑149 (MQ=255)
                                                            ccTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGAc                                                                                    >  1:67782/1‑149 (MQ=255)
                                                             cTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGAcc                                                                                   <  2:67782/149‑1 (MQ=255)
                                                                    aCCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAgcaac                                                                            >  7:321539/1‑149 (MQ=255)
                                                                    aCCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAgcaac                                                                            <  2:112825/149‑1 (MQ=255)
                                                                                tGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCg                                                                >  3:236432/1‑149 (MQ=255)
                                                                                tGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCg                                                                >  3:331939/1‑149 (MQ=255)
                                                                                      gTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCt                                                          >  1:278394/1‑149 (MQ=255)
                                                                                       tAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCt                                                          >  3:352557/1‑148 (MQ=255)
                                                                                               cTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTGCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCAc                                                 <  8:321539/149‑1 (MQ=255)
                                                                                                  tggtgTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAAcccc                                                                 >  7:155825/1‑130 (MQ=255)
                                                                                                  tggtgTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAAcccc                                                                 <  8:155825/130‑1 (MQ=255)
                                                                                                  tggtgTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGa                                              >  8:79723/1‑149 (MQ=255)
                                                                                                    gtgtTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTc                                                              <  1:356130/131‑1 (MQ=255)
                                                                                                    gtgtTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTc                                                              >  2:356130/1‑131 (MQ=255)
                                                                                                    gtgtTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATc                                            <  5:58998/149‑1 (MQ=255)
                                                                                                             tGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTgg                                                                                                    <  2:136647/84‑1 (MQ=255)
                                                                                                             tGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTgg                                                                                                    >  1:136647/1‑84 (MQ=255)
                                                                                                               acacCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGCGaa                                   >  5:107416/1‑147 (MQ=255)
                                                                                                               acacCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGCGaa                                   <  8:36009/147‑1 (MQ=255)
                                                                                                               acacCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGCGaa                                   <  6:107416/147‑1 (MQ=255)
                                                                                                                   cGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCCCCTCCACCGATCCTTGGCGACGGCTGc                             >  1:130933/1‑149 (MQ=255)
                                                                                                                          cTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCCCCTCCACCGATCCTTGGCGAAGGCTGCCTGGccc                      >  3:225617/1‑149 (MQ=255)
                                                                                                                              cTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGCGAAGGCTGCCTGGCCCGCt                   <  4:225617/148‑1 (MQ=255)
                                                                                                                              cTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGCGAAGGCTGCCTGGCCCGCTa                  <  7:79723/149‑1 (MQ=255)
                                                                                                                              cTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGCGAAGGCTGCCTGGCCCGCTa                  >  8:332647/1‑149 (MQ=255)
                                                                                                                               tgtgAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGCGAAGGCTGCCTGGCCCGCTAc                 >  8:91786/1‑149 (MQ=255)
                                                                                                                                gtgCGATTCCGGGCTGCTCCGCAGCCCTGTCCCGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGc                                      <  2:324210/127‑1 (MQ=255)
                                                                                                                                gtgAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGc                                      >  1:324210/1‑127 (MQ=255)
                                                                                                                                 tgAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCTATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGCGAAGGCTGCCTGGCCCGCTACGa               >  1:288257/1‑149 (MQ=255)
                                                                                                                                    gtgacagggCTGCGCAGCAGCCCGGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGCGAAGGCTGCCTGGCCCGCTACGAccc            >  1:158703/7‑149 (MQ=255)
                                                                                                                                    gaTTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGCGAAGGCTGCCTGGCCCGCTACGAccc            <  2:278394/149‑1 (MQ=255)
                                                                                                                                     aTTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGCGAAGGCTGCCTGGCCCGCTACGACCCt           <  4:352557/149‑1 (MQ=255)
                                                                                                                                       acagggCTGCGCAGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGCGAAGGCTGCCTGGCCCGCTACGACCCTg          >  5:196265/4‑148 (MQ=255)
                                                                                                                                       acagggCTGCGCAGCAGCCCGGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCAc                                                 <  8:123343/106‑1 (MQ=255)
                                                                                                                                       acagggCTGCGCAGCAGCCCGGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCAc                                                 >  7:123343/4‑109 (MQ=255)
                                                                                                                                         cGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGCGAAGGCTGCCTGGCCCGCTACGACCCTGAAg       >  5:182816/1‑149 (MQ=255)
                                                                                                                                           ggCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGCGAAGGCTGCCTGGCCCGCTACGACCCTGAAGcc     >  4:330214/1‑149 (MQ=255)
                                                                                                                                             ctgctgCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGCGAAGGCTGCCTGGCCCGCTACGACCCTGAAGCCCt   <  7:332647/149‑1 (MQ=255)
                                                                                                                                              tgctgcGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGCGAAGGCTGCCTGGCCCGCTACGACCCTGAAGCCCTg  >  6:64243/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CATGGTCCGCTTCGGCCGTGATGTGGAGAAGATGGTGCTGGCCCGTGGCCTGCGTTATCACCTGGAGGACCGCGTGCTGGTGCATGGTAACAAGACTGTGGTGTTCGACTGACACCGTCCGGCTTGCTGTGAGATTCCGGGCTGCTGCGCAGCCCTGTCACGACAGGGGCGCAGAGCGCCCCGGCAATCCTGGGTGAAACGGCAATGACCAAGCAACGCAACAACCCCGTCGCCTCACCTCCACCGATCCTTGGCGAAGGCTGCCTGGCCCGCTACGACCCTGAAGCCCTG  >  NC_002947/1556837‑1557127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑