Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,572,889 C→A 7.3% A337A (GCC→GCA)  pcaB → 3‑carboxy‑cis,cis‑muconate cycloisomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,572,8890CA 7.3% 169.5 / 4.2 55A337A (GCC→GCA) pcaB3‑carboxy‑cis,cis‑muconate cycloisomerase
Reads supporting (aligned to +/- strand):  ref base C (27/24);  new base A (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.51e-01

TCGGTGCCGCGACCCGCGTGCCGGGCCTGCTGTCGACGCTGTTCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGCCGAAGCGGTGAGCATCGTCCTCGCCCAGCGTCTGGGTCGCGACCGTGCCCA  >  NC_002947/1572753‑1573032
                                                                                                                                        |                                                                                                                                               
tCGGTGCCGCGACCCGCGTGCCGGGCCTGCTGTCGACGCTGTTCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCt                                                                                                                                     >  5:36199/1‑149 (MQ=255)
   gTGCCGCGACCCGCGTGCCGGGCCTGCTGTCGACGCTGTTCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCAGGGCGCCAgagacca                                                                                                                                  <  2:190330/149‑7 (MQ=255)
        gcgACCCGCGTGCCGGGCCTGCTGTCGACGCTGTTCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGa                                                                                                                              <  1:136690/148‑1 (MQ=255)
        gcgACCCGCGTGCCGGGCCTGCTGTCGACGCTGTTCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGa                                                                                                                              >  2:136690/1‑148 (MQ=255)
         cgACCCGCGTGCCGGGCCTGCTGTCGACGCTGTTCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGTCTCAAGTGAtt                                                                                                                            <  8:309042/149‑1 (MQ=255)
                 gTGCCGGGCCTGCTGTCGACGCTGTTCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAggg                                                                                                                    >  3:40486/1‑149 (MQ=255)
                  tGCCGGGCCTGCTTTCGACGCTGTTCTCAGCCATGCCTCAGGAGAACTAACGCAGCCTGGGCCTATGGAATGCCGAGTGGTAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGc                                                                                                                   <  3:61501/149‑1 (MQ=255)
                     cGGGCCTGCTGTCGACGCTGTTCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATg                                                                                                                >  2:205469/1‑149 (MQ=255)
                      gggCCTGCTGTCGACGCTGTTCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCaa                                                                                                                                  <  8:106675/130‑1 (MQ=255)
                      gggCCTGCTGTCGACGCTGTTCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCaa                                                                                                                                  >  7:106675/1‑130 (MQ=255)
                          ctgctgTCGACGCTGTTCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGACGAGGGCATGGAGGt                                                                                                           >  4:53071/1‑149 (MQ=255)
                            gctgTCGACGCTGTTCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTgg                                                                                                         <  4:94902/149‑1 (MQ=255)
                             ctgTCGACGCTGTTCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCCCTGGCGCAGGGCGCCAga                                                                                                                                       <  4:160921/118‑2 (MQ=255)
                             ctgTCGACGCTGTTCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCCCTGGCGCAGGGCGCCAga                                                                                                                                       >  3:160921/1‑117 (MQ=255)
                               gTCGACGCTGTTCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATg                                                                                                      >  2:155748/1‑149 (MQ=255)
                                   aCGCTGTTCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCcgc                                                                                                  >  1:4483/1‑149 (MQ=255)
                                       tGTTCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCcgcgcg                                                                                               >  2:159257/1‑148 (MQ=255)
                                         ttCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCAt                                                                                            >  7:195968/1‑149 (MQ=255)
                                         ttCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCAt                                                                                            >  7:292858/1‑149 (MQ=255)
                                            gCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTTGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTgg                                                                                                         <  8:234179/133‑1 (MQ=255)
                                            gCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATgcg                                                                                         >  3:43074/1‑149 (MQ=255)
                                            gCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTCGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTgg                                                                                                         >  7:234179/1‑133 (MQ=255)
                                                  aTGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTaa                                                                                   <  2:121419/149‑1 (MQ=255)
                                                         aGGAGCACGAACGCAGCCTGGGCCTATGGCATTCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGa                                                                             <  4:127293/148‑1 (MQ=255)
                                                         aGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGAc                                                                            <  1:333177/149‑1 (MQ=255)
                                                         aGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGAc                                                                            <  1:159257/149‑1 (MQ=255)
                                                         aGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGAc                                                                            <  4:43074/149‑1 (MQ=255)
                                                                cGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGAccc                                                                     <  8:259736/149‑1 (MQ=255)
                                                                 gAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGAccc                                                                     >  6:123282/1‑148 (MQ=255)
                                                                              gCCTATGGCATGCCGAGTGGGAAAGCCTGCCGGATATCTTCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTTGTGCTg                                                       <  5:67174/149‑1 (MQ=255)
                                                                               ccTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCTTGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTgg                                                      >  4:168080/1‑149 (MQ=255)
                                                                               ccTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTgg                                                      >  2:201029/1‑149 (MQ=255)
                                                                               ccTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTgg                                                      <  1:205469/149‑1 (MQ=255)
                                                                                 tATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGcc                                                    >  4:78011/1‑149 (MQ=255)
                                                                                   tGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGcc                                                    <  1:155748/147‑1 (MQ=255)
                                                                                   aggCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGcc                                                    <  3:343949/146‑1 (MQ=255)
                                                                                     gCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGCCGAAg                                                >  7:139298/1‑149 (MQ=255)
                                                                                      cATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAgg                                                                                                                     <  6:218709/79‑1 (MQ=255)
                                                                                      cATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAgg                                                                                                                     >  5:218709/1‑79 (MQ=255)
                                                                                            gAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGCCGAAGCGGTGAg                                         <  5:266664/149‑1 (MQ=255)
                                                                                             aGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGCCGAAGCGGTGAGc                                        <  3:78011/149‑1 (MQ=255)
                                                                                                 ggAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGCCGAAGCGGTGAGCATCg                                    >  6:29547/1‑149 (MQ=255)
                                                                                                  gAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGCCGAAGCGGTGAGCATCGt                                   >  3:141689/1‑149 (MQ=255)
                                                                                                     aCCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGCCGAAGCGGTGAGCATCGTCCt                                >  8:20852/1‑149 (MQ=255)
                                                                                                      cccTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGCCGAAGCGGTGAGCATCGTCCTc                               <  4:141689/149‑1 (MQ=255)
                                                                                                                        gcCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGCCGAAGCGGTGAGCATCGTCCTCGCCCAGCGTCTGGGTcgc             >  3:69173/1‑149 (MQ=255)
                                                                                                                             gTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGCCGAAGCGGTGAGCATCGTCCTCGCCCAGCGTCTGGGTCGCGAcc         <  8:139298/148‑1 (MQ=255)
                                                                                                                                tctGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTAGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGCCGAAGCGGTGAGCATCGTCCTCGCCCAGCGTCTGGGTCGCGACCGTGc     >  6:191149/1‑149 (MQ=255)
                                                                                                                                 ctGGCGCACGGCGCCAGGCTCAAGTGATTGCCGAGGGCATTGAGTTGGATGCCGCGAGCATGCGCCGTAACCTCGACCTGACACAAGGCCTCGTGCTGGCAGAAGCGGTGAGCATCGTCCTCGCCCAGCGTCTCGGTCGCGACCGTAcc    >  2:310947/1‑149 (MQ=255)
                                                                                                                                  tGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGCCGAAGCGGTGAGCATCGTCCTCGCCCAGCGTCTGGGTCGCGACCGTGccc   >  3:342783/1‑149 (MQ=255)
                                                                                                                                  tGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTCGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGCCGAAGCGGTGAGCATCGTCCTCGCCCAGCGTCTGGGTCGCGACCGTGccc   >  1:255087/1‑149 (MQ=255)
                                                                                                                                   ggCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGCCGAAGCGGTGAGCATCGTCCTCGCCCAGCGTCTGGGTCGCGACCGTGCcca  >  6:25718/1‑149 (MQ=255)
                                                                                                                                    gcgcCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCTTGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGCCGAAGCGGTGATCATCGTCCTCGCCCAGCGTCTGGGTCGCGACCGTGccc   <  3:168080/147‑1 (MQ=255)
                                                                                                                                    gcgcCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGCCGAAGCGGTGAGCATCGTCCTCGCCCAGCGTCTGGGTCGCGACCGTGccc   <  6:36199/147‑1 (MQ=255)
                                                                                                                                    gcgcCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGCCGAAGCGGTGAGCATCGTCCTCGCCCAGCGTCTGGGTCGCGACCGTGcc    <  5:123282/146‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                               
TCGGTGCCGCGACCCGCGTGCCGGGCCTGCTGTCGACGCTGTTCGCAGCCATGCCTCAGGAGCACGAACGCAGCCTGGGCCTATGGCATGCCGAGTGGGAAACCCTGCCGGATATCTGCTGCCTGGTCTCTGGCGCCCTGCGCCAGGCTCAAGTGATTGCCGAGGGCATGGAGGTGGATGCCGCGCGCATGCGCCGTAACCTCGACCTGACCCAAGGCCTGGTGCTGGCCGAAGCGGTGAGCATCGTCCTCGCCCAGCGTCTGGGTCGCGACCGTGCCCA  >  NC_002947/1572753‑1573032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑