Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,577,101 G→A 6.9% Q383* (CAG→TAG)  ttgC ← efflux pump outer membrane protein TtgC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,577,1010GA 6.9% 169.9 / 2.0 58Q383* (CAG→TAG) ttgCefflux pump outer membrane protein TtgC
Reads supporting (aligned to +/- strand):  ref base G (27/27);  new base A (2/2);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.98e-01

TCGAGGAAGGTCAGGTTGCTGTCAATCCCGATGCGGTAACGGCGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAGGCTGCCGGCGTTGAAGATCGGCAGGTTGATCTGCGGCTGGAACAGCCAGGTGCCCTGGCCGCCC  >  NC_002947/1576953‑1577246
                                                                                                                                                    |                                                                                                                                                 
tCGAGGAAGGTGAGGTTGCTGTCAATCCGGATGCGGTAACGGCGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTg                                                                                                                                                   <  4:80533/149‑1 (MQ=255)
  gAGGAAGGTCAGGTTGCTGTCAATCCCGATGCGGTAACGGCGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTgg                                                                                                                                                  >  2:242887/1‑148 (MQ=255)
    ggAAGGTCAGGTTGCTGTCAATCCCGATGCGGTAACGGCGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAg                                                                                                                                               >  6:49678/1‑149 (MQ=255)
     gAAGGTCAGGTTGCTGTCAATCCCGATGCGGTAACGGCGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAgg                                                                                                                                              <  6:22201/149‑1 (MQ=255)
        ggTCAGGTTGCTGTCAATCCCGATGCGGTAACGGCGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCg                                                                                                                                           >  1:19763/1‑149 (MQ=255)
            aGGTTGCTGTCAATCCCGATGCGGTAACGGCGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTg                                                                                                                                       <  6:2503/149‑1 (MQ=255)
              gTTGCTGTCAATCCCGATGCGGTAACGGCGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGa                                                                                                                                     >  2:348101/1‑149 (MQ=255)
              gTTGCTGTCAATCCCGATGCGGTAACGGCGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTAGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGa                                                                                                                                     >  2:352151/1‑149 (MQ=255)
               ttGCTGTCAATCCCGATGCGGTAACGGCGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGAt                                                                                                                                    <  1:242887/149‑1 (MQ=255)
                 gCTGTCAATCCCGATGCGGTAACGGCGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATg                                                                                                                                   <  3:58581/148‑1 (MQ=255)
                   tGTCAATCCCGATGCGGTAACGGCGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGGCTGGAGGGtt                                                                                                                                >  8:332474/1‑149 (MQ=255)
                     tCAATCCGGATGCGGTACCGGCGTTCGGCCAAGCGGTAGTTGTCCTGGTTCGCCTGCACCAGGTCGGGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGGCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGtt                                                                                                                                <  3:290016/147‑1 (MQ=255)
                     tCAATCCCGATGCGGTAACGGCGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGtttt                                                                                                                              <  1:259309/149‑1 (MQ=255)
                           ccGATGCGGTAACGGCGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTGCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTa                                                                                                                        <  6:263798/149‑1 (MQ=255)
                              aTGCGGTAACGGCGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACtt                                                                                                                     >  5:41660/1‑149 (MQ=255)
                                        ggCGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGtt                                                                                                            >  6:335272/1‑148 (MQ=255)
                                          cGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGAt                                                                                                         <  5:49678/149‑1 (MQ=255)
                                          cGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGAt                                                                                                         <  5:335272/149‑1 (MQ=255)
                                           gTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATg                                                                                                        >  1:106525/1‑149 (MQ=255)
                                                  cAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCt                                                                                                 >  3:224598/1‑149 (MQ=255)
                                                     gCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGt                                                                                                       <  8:211327/140‑1 (MQ=255)
                                                     gCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGt                                                                                                       >  7:211327/1‑140 (MQ=255)
                                                       ggTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGAtt                                                                                            <  6:41660/149‑1 (MQ=255)
                                                        gtagtaGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGAttt                                                                                           >  2:298145/1‑149 (MQ=255)
                                                           gtagtCCTGGGTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTc                                                                                         <  2:251827/148‑1 (MQ=255)
                                                             agtCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGa                                                                                       <  4:224598/148‑1 (MQ=255)
                                                              gtCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGGAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGa                                                                                       >  6:280058/1‑147 (MQ=255)
                                                              gtCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGGAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGa                                                                                       <  5:280058/147‑1 (MQ=255)
                                                              gtCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGag                                                                                      >  4:110184/1‑148 (MQ=255)
                                                                   ggTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTc                                                                                 >  6:308632/1‑148 (MQ=255)
                                                                    gTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGagtagt                                                                                  >  7:114359/1‑146 (MQ=255)
                                                                    gTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGagtagt                                                                                  <  8:114359/146‑1 (MQ=255)
                                                                        gCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAggct                                                                           >  7:134388/1‑149 (MQ=255)
                                                                         ccTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAggctg                                                                          >  5:217227/1‑149 (MQ=255)
                                                                          cTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAggctgg                                                                         <  2:19763/149‑1 (MQ=255)
                                                                              aCCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTAGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTc                                                                                         <  4:310854/129‑1 (MQ=255)
                                                                              aCCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTAGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTc                                                                                         <  4:65659/129‑1 (MQ=255)
                                                                              aCCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTAGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTc                                                                                         >  3:65659/1‑129 (MQ=255)
                                                                              aCCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTAGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTc                                                                                         >  3:310854/1‑129 (MQ=255)
                                                                               ccAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCACCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCttt                                                                     >  7:308925/1‑148 (MQ=255)
                                                                                cAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGa                                                                   <  1:352151/149‑1 (MQ=255)
                                                                                cAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGa                                                                   <  1:348101/149‑1 (MQ=255)
                                                                                 aGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAg                                                                  <  8:134388/149‑1 (MQ=255)
                                                                                   gTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAGGc                                                                >  4:190039/1‑149 (MQ=255)
                                                                                   gTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAGGc                                                                >  3:80827/1‑149 (MQ=255)
                                                                                                tGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGagta                                                                                    <  4:238893/116‑1 (MQ=255)
                                                                                                tGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGagta                                                                                    >  3:238893/1‑116 (MQ=255)
                                                                                                 gCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAGGCTGCCGGCGTTg                                                     >  8:89224/1‑146 (MQ=255)
                                                                                                  cAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAGGCTGCCGGCGTTGaa                                                   <  6:217227/147‑1 (MQ=255)
                                                                                                  cAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCACCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAGGCTGCCGGCGTTGaa                                                   <  8:308925/147‑1 (MQ=255)
                                                                                                         tctTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAGGCTGCCGGCGTTGAAGATCGGCAg                                          >  5:257003/1‑149 (MQ=255)
                                                                                                             cGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAGGCTGCCGGCGTTGAAGATCGGCAGGt                                        <  3:110184/147‑1 (MQ=255)
                                                                                                                     ttGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAGGCTGCCGGCGTTGAAGATCGGCAGGTTGATCTGCgg                              >  8:154500/1‑149 (MQ=255)
                                                                                                                                  ggCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAGGCTGCCGGCGTTGAAGATCGGCAGGTTGATCTGCGGCTGGAACAGCCAg                 <  1:246965/149‑1 (MQ=255)
                                                                                                                                  ggCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAGGCTGCCGGCGTTGAAGATCGGCAGGTTGATCTGCGGCTGGAACAGCCAg                 <  4:80827/149‑1 (MQ=255)
                                                                                                                                     cATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAGGCTGCCGGCGTTGAAGATCGGCAGGTTGATCTGCGGCTGGAACAGCCAGGTg              >  2:197015/1‑149 (MQ=255)
                                                                                                                                         ggAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAGGCTGCCGGCGTTGAAGATCGGCAGGTTGATCTGCGGCTGGAACAGCCAGGTGCCCt          <  2:106525/149‑1 (MQ=255)
                                                                                                                                           agaCTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAGGCTGCCGGCGTTGAAGATCGGCAGGTTGATCTGCGGCTGGAACAGCCAGGTGCCCTgg        <  7:89224/149‑1 (MQ=255)
                                                                                                                                             aCTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAGGCTGCCGGCGTTGAAGATCGGCAGGTTGATCTGCGGCTGGAACAGCCAGGTGCCCTGgcc      >  5:205325/1‑149 (MQ=255)
                                                                                                                                               ttCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAGGCTGCCGGCGTTGAAGATCGGCAGGTTGATCTGCGGCTGGAACAGCCAGGTGCCCTGgccgc    >  3:168045/1‑149 (MQ=255)
                                                                                                                                                    ggAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAGGCTGCCGGCGTTGAAGATCGGCAGGTTGATCTGCGGCTGGAACAGCCAGGTGCCCTGGCCGccc  >  7:59218/1‑146 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TCGAGGAAGGTCAGGTTGCTGTCAATCCCGATGCGGTAACGGCGTTCGGCCAAGCGGTAGTAGTCCTGGTTCGCCTGCACCAGGTCGCGCTGGGCCTGCAACTGCTCTTCGAAGGTCTTGCGTGCCGCCAGGCCATCGGAGACTTCCTGGAAGGCCGTCTGGATGGTTTTTTCGTACTTGGCGACGTTGATGTCCTTCTGGATTTTCGAGTAGTCCAGGCTGGCTTTGAGGCTGCCGGCGTTGAAGATCGGCAGGTTGATCTGCGGCTGGAACAGCCAGGTGCCCTGGCCGCCC  >  NC_002947/1576953‑1577246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑