Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,594,061 T→C 7.0% intergenic (‑259/+42) PP_1399 ← / ← kgtP hypothetical protein/alpha‑ketoglutarate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,594,0610TC 7.0% 204.3 / 4.8 72intergenic (‑259/+42)PP_1399/kgtPhypothetical protein/alpha‑ketoglutarate permease
Reads supporting (aligned to +/- strand):  ref base T (27/39);  new base C (2/3);  total (30/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

TGTATTTGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCT  >  NC_002947/1593913‑1594204
                                                                                                                                                     |                                                                                                                                               
tGTATTTGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCt                                                                                                                                                 >  6:218112/1‑149 (MQ=255)
  tATTTGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTcc                                                                                                                                               <  7:198954/149‑1 (MQ=255)
       gggCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGc                                                                                                                                           >  4:131636/1‑148 (MQ=255)
       gggCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGc                                                                                                                                           >  2:166112/1‑148 (MQ=255)
         gcgcGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAAt                                                                                                                                        >  7:271244/1‑149 (MQ=255)
            cgTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAATAGGGGCGCTCCATGCAATggg                                                                                                                                     <  2:225136/149‑1 (MQ=255)
                tGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGgcgc                                                                                                                                 <  3:283598/149‑1 (MQ=255)
                  tCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCAGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTATGAAGGAAAAGTGTGAATAGAAAAGGGGCGATCCATGCAATGGGGCGcc                                                                                                                                >  1:73530/1‑149 (MQ=255)
                  tCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCCCCATTGCATGGAGCGcc                                                                                                                                <  8:77529/148‑1 (MQ=255)
                        ttCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCttt                                                                                                                           >  2:130628/1‑147 (MQ=255)
                        ttCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTa                                                                                                                         <  1:130628/149‑1 (MQ=255)
                           aGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCCCCATTGCATGGAGCGCCCCttttcta                                                                                                                       <  5:19729/148‑4 (MQ=39)
                             gCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGcc                                                                                                                                <  4:120127/137‑1 (MQ=255)
                             gCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGcc                                                                                                                                >  3:120127/1‑137 (MQ=255)
                               cGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCAATGCAATGGGGCGCCCCTTTTAGTACTg                                                                                                                   >  4:295922/1‑148 (MQ=255)
                                 cAAGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgcca                                                                                                                <  7:298398/149‑1 (MQ=255)
                                 cAAGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgc                                                                                                                  >  1:54488/1‑147 (MQ=255)
                                  aaGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgccag                                                                                                               >  3:21688/1‑149 (MQ=255)
                                             gggAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGtt                                                                                                      <  1:122912/147‑1 (MQ=255)
                                             gggAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTAc                                                                                                    >  7:262794/1‑149 (MQ=255)
                                              ggAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTAc                                                                                                    <  5:47368/148‑1 (MQ=255)
                                                aacGTTGGCGCCCGC‑GCGAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACt                                                                                                   >  7:300820/3‑147 (MQ=255)
                                                aGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGTAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGAGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCa                                                                                                 <  6:328060/149‑1 (MQ=255)
                                                  cGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAg                                                                                                <  2:276031/148‑1 (MQ=255)
                                                   gTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGTCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTg                                                                                              <  2:54488/149‑1 (MQ=255)
                                                   gTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTg                                                                                              <  8:271244/149‑1 (MQ=255)
                                                   gTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTg                                                                                              <  5:218112/149‑1 (MQ=255)
                                                             gc‑gcCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTa                                                                                                                         >  7:275149/1‑112 (MQ=255)
                                                             gc‑gcCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTa                                                                                                                         <  8:275149/112‑1 (MQ=255)
                                                              c‑gcgAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGa                                                                                   <  5:208543/149‑1 (MQ=255)
                                                              c‑gcCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGa                                                                                   <  3:131636/149‑1 (MQ=255)
                                                                  cAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGtg                                                                                >  3:247994/1‑149 (MQ=255)
                                                                    aCGTTCTGCCAGCCATTGCAGCTTGATAAGGTTGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGtgct                                                                              <  3:295922/149‑1 (MQ=255)
                                                                      gTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGtgctgc                                                                            <  3:256924/149‑1 (MQ=255)
                                                                            ccagccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTgg                                                                        <  8:86731/147‑1 (MQ=255)
                                                                            ccagccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTg                                                                         <  4:247994/146‑1 (MQ=255)
                                                                            ccagccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTg                                                                         <  1:166112/146‑1 (MQ=255)
                                                                            ccagccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTg                                                                         <  4:21688/146‑1 (MQ=255)
                                                                              agccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTc                                                                    >  6:185424/1‑149 (MQ=255)
                                                                              agccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTc                                                                    >  6:188140/1‑149 (MQ=255)
                                                                               gccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCt                                                                   >  7:302605/1‑149 (MQ=255)
                                                                                      cAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATgg                                                            <  5:188140/149‑1 (MQ=255)
                                                                                                 ggTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCttt                                                                                                                           >  1:143889/1‑75 (MQ=255)
                                                                                                 ggTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCttt                                                                                                                           <  2:143889/75‑1 (MQ=255)
                                                                                                  gTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAAc                                                  >  7:61188/1‑147 (MQ=255)
                                                                                                  gTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAAc                                                  >  4:260562/1‑147 (MQ=255)
                                                                                                       aTTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCagcagc                                           <  3:260562/149‑1 (MQ=255)
                                                                                                          aaTAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGa                                         <  8:61188/148‑1 (MQ=255)
                                                                                                           aTAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCTCGAGTGCTGCTTGATGTCTTTCATGGGCGCGTAAACCAGCAGCGAGc                                       <  6:257865/149‑1 (MQ=255)
                                                                                                           aTAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAg                                        <  8:300820/148‑1 (MQ=255)
                                                                                                              gggCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAg                                     <  5:185424/148‑1 (MQ=255)
                                                                                                                       gaagGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAgg                           >  4:304505/1‑149 (MQ=255)
                                                                                                                       gaagGAAAAGTCTGAATAGAAAAGGGGCGCACCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAgg                           >  1:32497/1‑149 (MQ=255)
                                                                                                                        aagGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCATTCATGGTCGCGTAAACAAGCAGCGAGCAGGCGATACAGGc                          >  3:240016/1‑149 (MQ=255)
                                                                                                                         agGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCg                         <  7:44649/149‑1 (MQ=255)
                                                                                                                                gTCTTAATAGAAAAGTGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGt                  <  8:302605/149‑1 (MQ=255)
                                                                                                                                gTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTa                                                                                                                         >  7:172814/1‑46 (MQ=255)
                                                                                                                                gTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTa                                                                                                                         <  8:172814/46‑1 (MQ=255)
                                                                                                                                   tGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAg                                        <  7:109320/124‑1 (MQ=255)
                                                                                                                                   tGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAg                                        >  8:109320/1‑124 (MQ=255)
                                                                                                                                    gAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTc                                                                <  1:298587/99‑1 (MQ=255)
                                                                                                                                    gAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTc                                                                >  2:298587/1‑99 (MQ=255)
                                                                                                                                    gAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGAGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGtcc                >  3:268473/1‑145 (MQ=255)
                                                                                                                                     aaTAGAAAATGGGCGCTCCATGCAATTGGGCGCCCCTTTTAGTACTGCAAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCTTAAACCAGCAGCGAGCAGGCGATACAGTCTGTGACGTAcc               <  4:240016/147‑1 (MQ=255)
                                                                                                                                     aaTAGAAAAGGGGCGCTGCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGCTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCGGCGAGCAGGCGATACAGGCGGTGACGTAc                <  1:113273/146‑1 (MQ=255)
                                                                                                                                       tAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTa           <  8:262794/149‑1 (MQ=255)
                                                                                                                                          aaaaGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTAc                >  6:119051/1‑141 (MQ=255)
                                                                                                                                          aaaaGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTAc                <  5:119051/141‑1 (MQ=255)
                                                                                                                                           aaaGGGGCGCCCCATTGCATGGAGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGa                                 <  6:177934/123‑1 (MQ=38)
                                                                                                                                           aaaGGGGCGCCCCATTGCATGGAGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGa                                 >  5:177934/1‑123 (MQ=38)
                                                                                                                                                ggCGCCCCATTGCATGGAGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGct  >  6:197629/1‑149 (MQ=255)
                                                                                                                                                 gcgcTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTc                                                          <  6:113499/92‑1 (MQ=255)
                                                                                                                                                 gcgcTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTc                                                          >  5:113499/1‑92 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
TGTATTTGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGC‑GCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCT  >  NC_002947/1593913‑1594204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑