Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,668,724	C→G	8.8%	G56G (GGC→GGG)	rpsP →	30S ribosomal protein S16

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,668,724	0	C	G	8.8%	165.5 / 7.2	57	G56G (GGC→GGG)	rpsP	30S ribosomal protein S16
Reads supporting (aligned to +/- strand):  ref base C (29/23);  new base G (3/2);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

GATTTGCAGTACCAGCTGCTGTATCAACTGCAGCACCGACTATAGGAACGATGTTCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAGGGTGCACAGCCGTCTGAGCGTGTTGCTCAGCTGCTG  >  NC_002947/1668577‑1668859                                                                                                                                                     |                                                                                                                                         gATTTGCAGTACCAGCTGCTGTATCAACTGCAGCACCGACTATAGGAACGATGTTCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGcc                                                                                                                                         <  3:285260/149‑1 (MQ=255)  aTTTGCAGTACCAGCTGCTGTATCAACTGCAGCACCGACTATAGGAACGATGTTCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCg                                                                                                                                        >  3:233858/1‑149 (MQ=255)            ccAGCTGCTGTATCAACTGCAGCACCGACTATAGGAACGATGTTCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGa                                                                                                                               <  3:266458/148‑1 (MQ=255)            ccAGCTGCTGTATCAACTGCAGCACCGACTATAGGAACGATGTTCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAg                                                                                                                              >  7:92078/1‑149 (MQ=255)                   ctgTATCAACTGCAGCACCGACTATAGGAACGATGTTCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAg                                                                                                                       >  3:63366/1‑149 (MQ=255)                   ctgTATCAACTGCAGCACCGACTATAGGAACGATGTTCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAg                                                                                                                       >  6:165107/1‑149 (MQ=255)                          aaCTGCAGCACCGACTATAGGAACGATGTTCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTttctt                                                                                                                <  3:130027/149‑1 (MQ=255)                            cTGCAGCACCGACTATAGGAACGATGTTCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCCGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGTGCGTAGGTttctt‑c                                                                                                              >  6:262772/1‑148 (MQ=255)                               cagcaCCGACTATAGGAACGATGTTCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAAcc                                                                                                          <  7:179538/149‑1 (MQ=255)                                  caCCGACTATAGGAACGATGTTCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCaa                                                                                                       <  5:165107/149‑1 (MQ=255)                                    ccGACTATAGGAACGATGTTCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑Caa                                                                                                            <  3:121214/142‑1 (MQ=255)                                    ccGACTATAGGAACGATGTTCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑Caa                                                                                                            >  4:121214/1‑142 (MQ=255)                                              gAACGATGTTCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACGACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGgcg                                                                                           >  7:306939/1‑149 (MQ=255)                                                aCGATGTTCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGtt                                                                                                                                 >  5:343550/1‑110 (MQ=255)                                                aCGATGTTCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGtt                                                                                                                                 <  6:343550/110‑1 (MQ=255)                                                 cGATGTTCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTGTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAAGCGCTGCTGGCGcc                                                                                         >  6:290668/1‑148 (MQ=255)                                                      ttCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCCGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGt                                                                                    <  5:262772/148‑1 (MQ=255)                                                        cacaTGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGtt                                                                                   >  8:222162/1‑147 (MQ=255)                                                          caTGGTAACCATTCGTCTGGCCCGTTGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑Caa                                                                                                            <  4:64973/120‑1 (MQ=255)                                                          caTGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGAACG‑TGACCAACTCGAGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑Caa                                                                                                            >  3:64973/1‑120 (MQ=255)                                                           aTGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCt                                                                              >  7:129387/1‑149 (MQ=255)                                                              gTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTc                                                                           <  8:92078/149‑1 (MQ=255)                                                                   cATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTc                                                                       >  1:342350/1‑148 (MQ=255)                                                                    aTTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAg                                                                                <  1:119371/138‑1 (MQ=255)                                                                    aTTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAg                                                                                >  2:119371/1‑138 (MQ=255)                                                                    aTTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCaa                                                                     >  7:130462/1‑149 (MQ=255)                                                                    aTTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGCCaa                                                                     <  3:208796/149‑1 (MQ=255)                                                                             gCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTTCCAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCgg                                                                         <  1:246474/137‑1 (MQ=255)                                                                              cccGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAgcgc                                                           <  4:63366/149‑1 (MQ=255)                                                                                         tCGAAAAAGCGCCCATTCTACCAGCTGTCCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCgg                                                                         <  5:63041/124‑1 (MQ=255)                                                                                         tCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTg                                                >  6:181332/1‑149 (MQ=255)                                                                                         ctgAAAAAGCGACCATTCTACCACCTGACCG‑TTCCCAACTCGCGTAACGCCAGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACt                                                 <  7:318448/146‑1 (MQ=255)                                                                                           gAAAAAGCGCCCATTCTACCACCTGACCGTTGACCAACTCGCGTAACGCCCGTGCCGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTgg                                               >  1:26634/1‑149 (MQ=255)                                                                                            aaaaaGCGCCCATTCTACCCCCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CACCCCAAGCGCTGCTGGCGCCTAAGTTAAGCAGTCGGGACACCACGAGCTCGTACCCTACTgcta                                             >  8:318448/1‑146 (MQ=255)                                                                                                aGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGc                                         <  7:222162/149‑1 (MQ=255)                                                                                                 gcgcCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGcc                                                                                         <  5:228960/100‑1 (MQ=255)                                                                                                 gcgcCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGcc                                                                                         >  6:228960/1‑100 (MQ=255)                                                                                                   gcCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGa                                           <  5:201874/144‑1 (MQ=255)                                                                                                   gcCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGa                                           >  6:201874/1‑144 (MQ=255)                                                                                                   gcCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAg                                      <  2:342350/149‑1 (MQ=255)                                                                                                     ccATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTgg                                               <  8:308918/138‑1 (MQ=255)                                                                                                     ccATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTgg                                               >  7:308918/1‑138 (MQ=255)                                                                                                            accaccTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAGGGTGCACAg                             >  5:154103/1‑149 (MQ=255)                                                                                                               accTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAGGGTGCACAGCCg                          >  7:155941/1‑149 (MQ=255)                                                                                                                ccTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAGGGTGCACAGCCGt                         >  5:134832/1‑149 (MQ=255)                                                                                                                  tGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAGGGTGCACAGCCGTCt                       >  1:27073/1‑149 (MQ=255)                                                                                                                    aCCG‑TGACCAACTCGCGTAACGCCCGTGACTGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAGGGTGCACAGCCGTCTGa                     <  8:306939/149‑1 (MQ=255)                                                                                                                    aCCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAGGGt                                   <  5:261680/135‑1 (MQ=255)                                                                                                                    aCCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAGGGt                                   >  6:261680/1‑135 (MQ=255)                                                                                                                      cG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAGGGTGCACAGCCGTCTGAGc                   >  3:155413/1‑149 (MQ=255)                                                                                                                          gACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAGGGTGCACAGCCGTCTGAGCgtg                >  7:225635/1‑149 (MQ=255)                                                                                                                           aCCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGAGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAGGGTGCACAGCCGTCTGAGCgtg                <  2:27073/148‑1 (MQ=255)                                                                                                                                 caacgaaaCGGCCGTCACGGGCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAGGGTGCACAGCCGTCTGAGCGTGTTGCt           >  6:141932/7‑147 (MQ=255)                                                                                                                                     gaaaCGGCCGTCACGGGCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAGGGTGCACAGCCGTCTGAGCgtgt               <  3:136016/137‑1 (MQ=255)                                                                                                                                     gaaaCGGCCGTCACGGGCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAGGGTGCACAGCCGTCTGAGCgtgt               >  4:136016/3‑139 (MQ=255)                                                                                                                                          gCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAGGGTGCACAGCCGTCTGAGCGTGTTGCTCAGctgctg  >  4:340785/1‑147 (MQ=255)                                                                                                                                            ccGTCACGGGCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAGGGTGCACAGcc                           <  5:325721/120‑1 (MQ=255)                                                                                                                                            ccGTCACGGGCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAGGGTGCACAGcc                           >  6:325721/1‑120 (MQ=255)                                                                                                                                                     |                                                                                                                                         GATTTGCAGTACCAGCTGCTGTATCAACTGCAGCACCGACTATAGGAACGATGTTCACATGGTAACCATTCGTCTGGCCCGTGGCGGCTCGAAAAAGCGCCCATTCTACCACCTGACCG‑TGACCAACTCGCGTAACGCCCGTGACGGCCGTTTCGTTGAGCGCGTAGGTTTCTT‑CAACCCAATCGCTGCTGGCGCCGAAGTTAAGCTGTCGGTCAACCAAGAGCGCGTCACCTACTGGCTGAGCCAGGGTGCACAGCCGTCTGAGCGTGTTGCTCAGCTGCTG  >  NC_002947/1668577‑1668859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑