Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,706,340	C→A	5.5%	A135A (GCC→GCA) ‡	PP_1500 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,706,340	0	C	A	5.5%	235.6 / 2.5	73	A135A (GCC→GCA) ‡	PP_1500	lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (34/35);  new base A (2/2);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

GCGGTAACCTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCGGC  >  NC_002947/1706192‑1706486                                                                                                                                                     |                                                                                                                                                   gcggTAACCTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGcc                                                                                                                                                    <  5:151313/149‑1 (MQ=255)   gtaaaCCTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACAAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGATGTGTACCAGGACATTGCTGCAGCCATGGCCTg                                                                                                                                                  <  6:204072/146‑1 (MQ=255)         cTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTgca                                                                                                                                            <  7:204715/149‑1 (MQ=255)         cTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTg                                                                                                                                              >  3:93269/1‑147 (MQ=255)         cTGCCAGGGCACCTGGGGGGGAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTgca                                                                                                                                            <  8:70891/149‑1 (MQ=255)           gCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTgcag                                                                                                                                           >  5:115776/1‑148 (MQ=255)           gCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAGGCCATGGCTgcag                                                                                                                                           >  1:155/1‑148 (MQ=255)            ccAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAGGCCATGGCTgcagc                                                                                                                                          <  2:155/148‑1 (MQ=255)             cAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGcagcag                                                                                                                                        <  5:158681/149‑1 (MQ=255)              aGGGCACCTGGGGGCTAGCTAATGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGAACGATTACCGCGGCTATGGCATGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGAAATTGCTGCAGACATGGACTGGCTGCAGCAgg                                                                                                                                       >  6:286343/1‑149 (MQ=255)                ggCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTgcagca                                                                                                                                         >  2:28840/1‑145 (MQ=255)                ggCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTgcagca                                                                                                                                         <  1:28840/145‑1 (MQ=255)                        gggggTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAg                                                                                                                               <  1:86844/147‑1 (MQ=255)                             tAGCTACTGGCTGCCGGAGCAGGGCTACCAGTTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAgg                                                                                                                        <  6:21431/149‑1 (MQ=255)                               gCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAggg                                                                                                                       <  6:274272/148‑1 (MQ=255)                                      gCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGc                                                                                                               >  1:168990/1‑149 (MQ=255)                                        tGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCCGGCGCCCGAGGTCAAGGGCAAGCCGCTg                                                                                                             <  4:93269/149‑1 (MQ=255)                                        tGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTg                                                                                                             >  5:92169/1‑149 (MQ=255)                                                 aGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTActgctg                                                                                                    >  4:93990/1‑149 (MQ=255)                                                 aGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTActgctg                                                                                                    >  4:334811/1‑149 (MQ=255)                                                 aGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTActgctg                                                                                                    <  8:182411/149‑1 (MQ=255)                                                  gggCTACCAGGTGCTGATGATCGATTACAGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGAAGGCGCCCGAGGTCAAGGGCAAGCAGCTGGTACTGCTgg                                                                                                   >  8:325990/1‑149 (MQ=255)                                                     cTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGATCAAGGGCAAGCCGCTGGTACTGCTgggg                                                                                                 >  2:236069/1‑148 (MQ=255)                                                        ccAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCt                                                                                                                                               <  5:152848/99‑1 (MQ=255)                                                        ccAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCt                                                                                                                                               >  6:152848/1‑99 (MQ=255)                                                            gTGCTGATGATCGATTACCGCGTCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCt                                                                                         <  2:194756/149‑1 (MQ=255)                                                             tGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCACGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCaga                                                                                             >  2:59298/1‑144 (MQ=255)                                                             tGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCACGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCaga                                                                                             <  1:59298/144‑1 (MQ=255)                                                                tgatgaTCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGc                                                                                     >  1:341344/1‑149 (MQ=255)                                                                 gatgatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGc                                                                                                               >  2:333541/1‑122 (MQ=255)                                                                 gatgatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGc                                                                                                               <  1:333541/122‑1 (MQ=255)                                                                   tgatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGt                                                                                  <  2:341344/149‑1 (MQ=255)                                                                    gatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCg                                                                                    <  2:183903/146‑1 (MQ=255)                                                                    gatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCg                                                                                    >  1:183903/1‑146 (MQ=255)                                                                    gatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGt                                                                                  <  2:168990/148‑1 (MQ=255)                                                                         aTTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCt                                                                                                              <  1:132346/115‑1 (MQ=255)                                                                         aTTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCt                                                                                                              >  2:132346/1‑115 (MQ=255)                                                                              cgcgGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCcat                                                                       <  1:92522/149‑1 (MQ=255)                                                                              cgcgGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCcat                                                                       >  3:295066/1‑149 (MQ=255)                                                                               gcgGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCcatt                                                                      >  4:39820/1‑149 (MQ=255)                                                                                cgGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCcatt                                                                      >  2:155872/1‑148 (MQ=255)                                                                                  gCTATGGGTTGGCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGAGATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCcattcat                                                                   <  4:166257/149‑1 (MQ=255)                                                                                  gCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCcattcat                                                                   >  3:166257/1‑149 (MQ=255)                                                                                     aTGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCCAGGTCAAGGTCAAGCCGCACGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTAc                                                                >  1:103890/1‑149 (MQ=255)                                                                                        gCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTCCAGCCATTGCCTGGCTGCAGCAGGCGCCCGAGGTCAACGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCt                                                              <  5:286343/148‑1 (MQ=255)                                                                                            gTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTggcg                                                          <  6:115776/148‑1 (MQ=255)                                                                                               tcAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGcc                                                       >  3:205074/1‑148 (MQ=255)                                                                                               tcAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGcc                                                       >  1:336617/1‑148 (MQ=255)                                                                                                  gggCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCAcc                                                   <  1:155872/149‑1 (MQ=255)                                                                                                   ggCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCAccc                                                  >  7:338511/1‑149 (MQ=255)                                                                                                      cAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGa                                               <  6:92169/149‑1 (MQ=255)                                                                                                       aGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGag                                              <  3:334811/149‑1 (MQ=255)                                                                                                         ccGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGagca                                            >  4:332581/1‑149 (MQ=255)                                                                                                         ccGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGATCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCTATGGCCATTCATTACCTGGCGGCCCACCCCGagca                                            <  1:236069/149‑1 (MQ=255)                                                                                                                tGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGG‑CCACCCCGAGCAGCGTCAgc                                    >  8:46526/1‑149 (MQ=255)                                                                                                                  ccGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAgcg                                   >  4:176313/1‑149 (MQ=255)                                                                                                                       ggTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCa                              <  3:93990/149‑1 (MQ=255)                                                                                                                             ccAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGG‑CCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCt                       <  7:46526/149‑1 (MQ=255)                                                                                                                              cAGGAGATTGGTGGAGCCATGGGCTGGCTGCAGCAGGCGCCCGAGGTCTAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTAACTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGccc                        <  2:103890/148‑1 (MQ=255)                                                                                                                              cAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCt                       >  8:187695/1‑149 (MQ=255)                                                                                                                              cAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCt                       >  5:50592/1‑149 (MQ=255)                                                                                                                                ggACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTgg                     >  4:10876/1‑149 (MQ=255)                                                                                                                                    aTTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGtt                 >  6:264226/1‑149 (MQ=255)                                                                                                                                    aTTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGtt                 >  7:306133/1‑149 (MQ=255)                                                                                                                                    aTTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGtt                 >  7:9074/1‑149 (MQ=255)                                                                                                                                       gctgcAGCCAGGCCATGGCTGCCGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCAGCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGtt                 >  8:250728/1‑146 (MQ=255)                                                                                                                                       gctgcAGCCAGGCCATGGCTGCCGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGtt                 <  7:250728/146‑1 (MQ=255)                                                                                                                                          gcAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATg            <  4:205074/148‑1 (MQ=255)                                                                                                                                           cAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATg            <  3:39820/147‑1 (MQ=255)                                                                                                                                           cAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGgt          <  7:187695/149‑1 (MQ=255)                                                                                                                                           cAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGgt          <  8:9074/149‑1 (MQ=255)                                                                                                                                                   gCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCGGc  <  6:50592/149‑1 (MQ=255)                                                                                                                                                   gCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCGGc  >  6:144689/1‑149 (MQ=255)                                                                                                                                                   gCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCGGc  <  2:336617/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GCGGTAACCTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCGGC  >  NC_002947/1706192‑1706486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑