| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,709,447 | C→G | 6.5% | T22T (ACC→ACG) ‡ | ppc → | phosphoenolpyruvate carboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,709,447 | 0 | C | G | 6.5% | 194.1 / 3.5 | 61 | T22T (ACC→ACG) ‡ | ppc | phosphoenolpyruvate carboxylase |
| Reads supporting (aligned to +/- strand): ref base C (27/30); new base G (2/2); total (29/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.98e-01 | |||||||||||
GCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGACCTGGCCGAAGAAGACCTGCTGCCCGTAGC > NC_002947/1709300‑1709587 | gCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCa > 8:4165/1‑149 (MQ=255) tgGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCGTATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCAt < 1:98776/147‑1 (MQ=255) cACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCaa < 2:180767/149‑1 (MQ=255) ctctTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACa < 1:27346/149‑1 (MQ=255) ggggATACCGCTCGCTCCTGCACAGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATg < 1:17453/148‑1 (MQ=255) aTACCGCTCGCTCCTGCACAGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGAt < 2:54629/149‑1 (MQ=255) ccGCTCGCTCCTGCACAGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGc < 8:116112/148‑1 (MQ=255) ccGCTCGCTCCTGCACAGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCg > 2:292702/1‑149 (MQ=255) cgctcgctCCTGCACAGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAATGGTTTCGCCaag < 1:187793/134‑2 (MQ=255) cgctcgctCCTGCACAGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAATGGTTTCGCCaag > 2:187793/1‑133 (MQ=255) cgctcgctCCTGCACAGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGt < 3:196384/149‑1 (MQ=255) gctcCTGCACAGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTcc < 1:292702/147‑1 (MQ=255) gctcCTGCACAGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCt < 8:231353/148‑1 (MQ=255) tGCACAGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGa > 2:130918/1‑148 (MQ=255) gCACAGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTc < 1:106972/140‑1 (MQ=255) gCACAGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTc > 2:106972/1‑140 (MQ=255) aGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAATGGTTTCGCCaag < 2:138712/119‑2 (MQ=255) aGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAATGGTTTCGCCaag > 1:138712/1‑118 (MQ=255) aGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATc > 6:97758/1‑148 (MQ=255) gggCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGa < 3:258261/149‑1 (MQ=255) gggCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGa < 5:97758/149‑1 (MQ=255) ggCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAg < 5:93276/149‑1 (MQ=255) ggCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAg < 1:81705/149‑1 (MQ=255) ggCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAg > 2:81705/1‑149 (MQ=255) ggCGCGCCGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTTGTTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAg < 7:219139/149‑1 (MQ=255) gcTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACa > 4:311902/1‑148 (MQ=255) gCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCca > 6:18249/1‑149 (MQ=255) aTGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAg < 1:91239/149‑1 (MQ=255) aaCGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTACTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCAGCCACAGTGCCAAGGcc > 4:292648/1‑148 (MQ=255) cGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGAc < 2:301680/149‑1 (MQ=255) ggAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGAcc > 1:85456/1‑149 (MQ=255) gagagaGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCg < 4:158557/149‑1 (MQ=255) gcGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGTTGAACTACTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCAGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGag < 3:292648/148‑1 (MQ=255) ccGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGag > 7:68200/1‑149 (MQ=255) ggATTTCG‑ATGTGGGTTTGCGTGAAGATGTCGATGTGTTGGGTGTACTGCTTGGCGAAACCTTTGGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAggt < 1:310598/104‑2 (MQ=255) cGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGa > 2:305908/1‑105 (MQ=255) cGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGa < 1:305908/105‑1 (MQ=255) cGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGagc < 1:130918/149‑1 (MQ=255) cGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGag > 5:55301/1‑148 (MQ=255) atCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCt > 7:275007/1‑149 (MQ=255) cG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTc > 8:284669/1‑59 (MQ=255) g‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAg < 8:275007/149‑1 (MQ=255) tttGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGAccc > 1:139302/1‑149 (MQ=255) gCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTg < 2:85456/148‑1 (MQ=255) gTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCg > 1:135863/1‑149 (MQ=255) tGAAGATGTCCATGTGTTTGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCg > 8:303510/1‑148 (MQ=255) aGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGAcc > 3:321153/1‑149 (MQ=255) gTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGACCTGGc > 6:41336/1‑149 (MQ=255) tCCATGTGTTTGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGACCTGGc < 7:303510/148‑1 (MQ=255) tCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGACCTGGcc > 2:56931/1‑149 (MQ=255) tCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGACCTGGcc > 7:187416/1‑149 (MQ=255) aTGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGACCTGGCCgaa < 8:187416/149‑1 (MQ=255) tgtgTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGACCTGGCCg > 4:320637/1‑146 (MQ=255) tgtTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGACCTGGCCgaaga < 7:4165/148‑1 (MQ=255) ttGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGACCTGGcc > 6:248973/1‑141 (MQ=255) ttGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGACCTGGcc < 5:248973/141‑1 (MQ=255) ttGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGACCTGGCCGaagaag < 5:41336/148‑1 (MQ=255) gTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGACCTGGCCGAAGAAGACctg < 1:56931/149‑1 (MQ=255) tGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGACCTGGCCGAAGAAGACctgc > 3:15147/1‑149 (MQ=255) tGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGACCTGGCCGAAGAAGACCTGCTGccc > 2:35086/1‑149 (MQ=255) cGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGACCTGGCCGAAGAAGACCTGCTGCCCGTAGc > 3:189976/1‑147 (MQ=255) | GCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGGCTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCG‑ATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGACCTGGCCGAAGAAGACCTGCTGCCCGTAGC > NC_002947/1709300‑1709587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |