Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,732,525 A→T 10.2% Y449N (TAC→AAC)  PP_1526 ← beta‑(1‑3)‑glucosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,732,5250AT10.2% 107.0 / 3.7 39Y449N (TAC→AAC) PP_1526beta‑(1‑3)‑glucosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (17/18);  new base T (2/2);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGAC  >  NC_002947/1732378‑1732669
                                                                                                                                                   |                                                                                                                                                
tCAGCGCGTCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTACGCAGTGGCCCTTGAGCGGCTCCCACACGGCCGCGTCCTTGGTGTTGTTGTCGCTCACCAGCACTTCGTACTCCGGGTAg                                                                                                                                                 >  8:3049/1‑149 (MQ=255)
tCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAg                                                                                                                                                 >  5:53208/1‑149 (MQ=255)
  aGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTa                                                                                                                                                  >  1:31919/1‑146 (MQ=255)
  aGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTa                                                                                                                                                  <  2:31919/146‑1 (MQ=255)
  aGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTc                                                                                                                                               >  7:160/1‑149 (MQ=255)
            ccTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGggcggc                                                                                                                                      >  1:139508/1‑148 (MQ=255)
              ttGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTTGTCCAGGGCGGCCAg                                                                                                                                   >  4:324273/1‑149 (MQ=255)
              ttGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTTGTCCAGGGCGGCCAg                                                                                                                                   >  4:319084/1‑149 (MQ=255)
                       gCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAg                                                                                                                          <  2:139508/149‑1 (MQ=255)
                          aGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGt                                                                                                                       <  7:3049/149‑1 (MQ=255)
                            tGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCt                                                                                                                     <  6:310630/149‑1 (MQ=255)
                              gcgcGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGt                                                                                                                   <  8:81069/149‑1 (MQ=255)
                                   aCGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCAc                                                                                                              <  6:53208/149‑1 (MQ=255)
                                          agaaCTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCAtctc                                                                                                        <  6:16261/148‑1 (MQ=255)
                                           gaaCTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAg                                                                                                      <  8:160/149‑1 (MQ=255)
                                                            cAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCAc                                                                                     >  5:78089/1‑149 (MQ=255)
                                                            cAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCAc                                                                                     <  3:180892/149‑1 (MQ=255)
                                                                     cGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATg                                                                             >  4:176551/1‑148 (MQ=255)
                                                                      gCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGt                                                                                          <  1:125427/134‑1 (MQ=255)
                                                                      gCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGt                                                                                          >  2:125427/1‑134 (MQ=255)
                                                                      gCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCa                                                                           >  6:18273/1‑149 (MQ=255)
                                                                                       gCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTg                                                                                           >  8:277895/1‑116 (MQ=255)
                                                                                       gCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTg                                                                                           <  7:277895/116‑1 (MQ=255)
                                                                                       gCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCgg                                                          >  8:326685/1‑149 (MQ=255)
                                                                                         tCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCgg                                                          >  3:122336/1‑147 (MQ=255)
                                                                                           ccACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAgg                                                      >  1:126796/1‑149 (MQ=255)
                                                                                           ccACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAgg                                                      >  7:221655/1‑149 (MQ=255)
                                                                                            cacaCGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAgg                                                      >  7:103558/1‑148 (MQ=255)
                                                                                                  gCCGGGTCCTTGGTGTTGTTGGCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCTCCGACACCTTGGGCCGGTAGGCAGTGTc                                               <  7:286561/149‑1 (MQ=255)
                                                                                                     gggTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGc                                            <  8:103558/149‑1 (MQ=255)
                                                                                                       gTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCt                                          <  5:18273/149‑1 (MQ=255)
                                                                                                                 ttgttgtCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAg                                <  6:78089/149‑1 (MQ=255)
                                                                                                                    ttgtCGATCACCAGCACTTCGTAGTCGGGGTTGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGaa                             <  3:319084/149‑1 (MQ=255)
                                                                                                                    ttgtCGATCACCAGCACTTCGTAGTCGGGGTTGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGaa                             <  3:324273/149‑1 (MQ=255)
                                                                                                                               cAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCgcggcggc                  >  7:152610/1‑149 (MQ=255)
                                                                                                                                aGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCg                                                           >  3:107151/1‑107 (MQ=255)
                                                                                                                                aGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCg                                                           <  4:107151/107‑1 (MQ=255)
                                                                                                                                              ggggTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGa   <  8:221655/149‑1 (MQ=255)
                                                                                                                                              ggggTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGa   <  3:176551/149‑1 (MQ=255)
                                                                                                                                              ggggTAGTACAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCTGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGa   >  6:318184/1‑149 (MQ=255)
                                                                                                                                               gggTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGAc  <  7:326685/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGAC  >  NC_002947/1732378‑1732669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑