Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,835,647 G→A 10.0% intergenic (‑89/+43) PP_1639 ← / ← yohC SprT family protein/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,835,6470GA10.0% 133.3 / 4.9 50intergenic (‑89/+43)PP_1639/yohCSprT family protein/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (22/23);  new base A (2/3);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

AAAAGGCTTCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGA  >  NC_002947/1835501‑1835791
                                                                                                                                                  |                                                                                                                                                
aaaaGGCTTCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTgc                                                                                                                                                 <  7:298477/148‑1 (MQ=255)
 aaaGGCTTCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCCCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTgcgg                                                                                                                                               >  4:188555/1‑149 (MQ=255)
    ggCTTCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGcggcgg                                                                                                                                            <  3:151517/149‑1 (MQ=255)
       ttCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTgcggc                                                                                                                                              <  1:159149/144‑1 (MQ=255)
       ttCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTgcggc                                                                                                                                              >  2:159149/1‑144 (MQ=255)
       ttCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGAGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGccc                                                                                                                                         >  5:338998/1‑149 (MQ=255)
         cGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGccc                                                                                                                                         <  4:64294/147‑1 (MQ=255)
          ggCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCt                                                                                                                                       <  1:230483/148‑1 (MQ=255)
          ggCTTGCTGGTAACAGGTTGCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCt                                                                                                                                       <  3:145207/148‑1 (MQ=255)
           gCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTgg                                                                                                                                     <  6:338998/149‑1 (MQ=255)
                 tGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCGCAAGGCGGCCTCTg                                                                                                                                      >  5:172123/1‑142 (MQ=255)
                 tGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCGCAAGGCGGCCTCTg                                                                                                                                      <  6:172123/142‑1 (MQ=255)
                  ggTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGc                                                                                                                              >  8:3276/1‑149 (MQ=255)
                   gTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGGGGCCGCCGCAAGGCGGCCTCTGGCTgtgcgc                                                                                                                             <  2:85071/149‑6 (MQ=38)
                   gTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGcc                                                                                                                             >  2:118049/1‑149 (MQ=255)
                      aCAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCa                                                                                                                          <  7:3276/149‑1 (MQ=255)
                        aGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAgt                                                                                                                        >  8:154603/1‑149 (MQ=255)
                          gTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTgg                                                                                                                                     <  4:113039/134‑1 (MQ=255)
                          gTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTgg                                                                                                                                     >  3:113039/1‑134 (MQ=255)
                          gTTTCGACGAGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGAAGCCCCAgtga                                                                                                                      >  6:137021/1‑148 (MQ=255)
                                 cgcgTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAgtg                                                                                                                       <  6:225325/141‑1 (MQ=255)
                                 cgcgTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAgtg                                                                                                                       >  5:225325/1‑141 (MQ=255)
                                    gttgttTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTc                                                                                                            >  2:206857/1‑149 (MQ=255)
                                       gttTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGAt                                                                                                                                >  5:199232/1‑126 (MQ=255)
                                       gttTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGAt                                                                                                                                <  6:199232/126‑1 (MQ=255)
                                            aGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTa                                                                                                     <  1:118049/148‑1 (MQ=255)
                                                gctCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAACCAGGGGCCGCCGCAAGGCGGCCTCTggct                                                                                                                                   >  4:24941/1‑112 (MQ=37)
                                                gctCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAACCAGGGGCCGCCGCAAGGCGGCCTCTggct                                                                                                                                   <  3:24941/114‑3 (MQ=37)
                                                 ctCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGTCCCAGTGTTGGTTCGGTCTGTTCTAGTTGgt                                                                                               >  7:279744/1‑149 (MQ=255)
                                                      gCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTAGGTATGTTCTACTTGGTGTAGa                                                                                          >  6:240998/1‑149 (MQ=255)
                                                                        ccTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGtt                                                                                                                 >  8:147090/1‑108 (MQ=255)
                                                                        ccTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGtt                                                                                                                 <  7:147090/108‑1 (MQ=255)
                                                                         cTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTc                                                                        <  5:240998/148‑1 (MQ=255)
                                                                                 gCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATaa                                                               >  8:103791/1‑149 (MQ=255)
                                                                                          cGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAg                                                      >  5:346420/1‑149 (MQ=255)
                                                                                            cAAGCGCAGGTGGCTTGCGGAGTCACGTGTCAGCGGACTGCCAGAGGCCGCCTTGCGGGGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTTGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCg                                                    <  3:188555/149‑1 (MQ=255)
                                                                                               gcgcAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGc                                                  >  3:190279/1‑148 (MQ=255)
                                                                                                   aGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGa                                             <  7:43287/149‑1 (MQ=255)
                                                                                                   aGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGa                                             >  2:162799/1‑149 (MQ=255)
                                                                                                     gTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGAtt                                           <  4:190279/149‑1 (MQ=255)
                                                                                                         cTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGcca                                       >  8:331058/1‑149 (MQ=255)
                                                                                                          ttGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTc                                                                                                       >  6:80040/1‑84 (MQ=255)
                                                                                                          ttGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTc                                                                                                       <  5:80040/84‑1 (MQ=255)
                                                                                                               gAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGTCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGta                                 <  8:279744/149‑1 (MQ=255)
                                                                                                                      cGGTCAGCGCACAGCCAGAGGCCGCCTTGGGGCGGCCCCTGGTTGATGCCCCAGTGTTGTTTCGGTCTGTTCTAGTTGGTGTAGACGGTCCCCACGCCCAGTCCCGAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCaa                          <  5:137021/149‑1 (MQ=255)
                                                                                                                         tCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACTGTAGCGGCCATGATTGCCACCAGTACTACCAAGcc                       <  7:331058/149‑1 (MQ=255)
                                                                                                                           aGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGccc                      <  1:206857/148‑1 (MQ=255)
                                                                                                                           aGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCa                     >  4:231821/1‑149 (MQ=255)
                                                                                                                                  aGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAg              <  7:154603/149‑1 (MQ=255)
                                                                                                                                  aGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAg              >  6:190819/1‑149 (MQ=255)
                                                                                                                                         ggCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCGTGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAg       >  7:205823/1‑149 (MQ=255)
                                                                                                                                            cgccTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTg    >  5:287585/1‑149 (MQ=255)
                                                                                                                                             gccTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTgg   <  6:346420/149‑1 (MQ=255)
                                                                                                                                              ccTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGga  <  4:94214/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
AAAAGGCTTCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGA  >  NC_002947/1835501‑1835791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑