Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,835,656 C→T 10.0% intergenic (‑98/+34) PP_1639 ← / ← yohC SprT family protein/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,835,6560CT10.0% 128.6 / 6.0 50intergenic (‑98/+34)PP_1639/yohCSprT family protein/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (22/23);  new base T (2/3);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01

TTCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGC  >  NC_002947/1835508‑1835802
                                                                                                                                                    |                                                                                                                                                   
ttCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGAGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGccc                                                                                                                                                     >  5:338998/1‑149 (MQ=255)
  cGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGccc                                                                                                                                                     <  4:64294/147‑1 (MQ=255)
   ggCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCt                                                                                                                                                   <  1:230483/148‑1 (MQ=255)
   ggCTTGCTGGTAACAGGTTGCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCt                                                                                                                                                   <  3:145207/148‑1 (MQ=255)
    gCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTgg                                                                                                                                                 <  6:338998/149‑1 (MQ=255)
          tGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCGCAAGGCGGCCTCTg                                                                                                                                                  >  5:172123/1‑142 (MQ=255)
          tGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCGCAAGGCGGCCTCTg                                                                                                                                                  <  6:172123/142‑1 (MQ=255)
           ggTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGc                                                                                                                                          >  8:3276/1‑149 (MQ=255)
            gTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGGGGCCGCCGCAAGGCGGCCTCTGGCTgtgcgc                                                                                                                                         <  2:85071/149‑6 (MQ=38)
            gTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGcc                                                                                                                                         >  2:118049/1‑149 (MQ=255)
               aCAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCa                                                                                                                                      <  7:3276/149‑1 (MQ=255)
                 aGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAgt                                                                                                                                    >  8:154603/1‑149 (MQ=255)
                   gTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTgg                                                                                                                                                 >  3:113039/1‑134 (MQ=255)
                   gTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTgg                                                                                                                                                 <  4:113039/134‑1 (MQ=255)
                   gTTTCGACGAGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGAAGCCCCAgtga                                                                                                                                  >  6:137021/1‑148 (MQ=255)
                          cgcgTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAgtg                                                                                                                                   <  6:225325/141‑1 (MQ=255)
                          cgcgTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAgtg                                                                                                                                   >  5:225325/1‑141 (MQ=255)
                             gttgttTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTc                                                                                                                        >  2:206857/1‑149 (MQ=255)
                                gttTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGAt                                                                                                                                            <  6:199232/126‑1 (MQ=255)
                                gttTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGAt                                                                                                                                            >  5:199232/1‑126 (MQ=255)
                                     aGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTa                                                                                                                 <  1:118049/148‑1 (MQ=255)
                                         gctCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAACCAGGGGCCGCCGCAAGGCGGCCTCTggct                                                                                                                                               >  4:24941/1‑112 (MQ=37)
                                         gctCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAACCAGGGGCCGCCGCAAGGCGGCCTCTggct                                                                                                                                               <  3:24941/114‑3 (MQ=37)
                                          ctCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGTCCCAGTGTTGGTTCGGTCTGTTCTAGTTGgt                                                                                                           >  7:279744/1‑149 (MQ=255)
                                               gCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTAGGTATGTTCTACTTGGTGTAGa                                                                                                      >  6:240998/1‑149 (MQ=255)
                                                                 ccTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGtt                                                                                                                             >  8:147090/1‑108 (MQ=255)
                                                                 ccTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGtt                                                                                                                             <  7:147090/108‑1 (MQ=255)
                                                                  cTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTc                                                                                   <  5:240998/148‑1 (MQ=255)
                                                                          gCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATaa                                                                          >  8:103791/1‑149 (MQ=255)
                                                                                   cGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAg                                                                 >  5:346420/1‑149 (MQ=255)
                                                                                     cAAGCGCAGGTGGCTTGCGGAGTCACGTGTCAGCGGACTGCCAGAGGCCGCCTTGCGGGGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTTGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCg                                                               <  3:188555/149‑1 (MQ=255)
                                                                                        gcgcAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGc                                                             >  3:190279/1‑148 (MQ=255)
                                                                                            aGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGa                                                        <  7:43287/149‑1 (MQ=255)
                                                                                            aGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGa                                                        >  2:162799/1‑149 (MQ=255)
                                                                                              gTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGAtt                                                      <  4:190279/149‑1 (MQ=255)
                                                                                                  cTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGcca                                                  >  8:331058/1‑149 (MQ=255)
                                                                                                   ttGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTc                                                                                                                   >  6:80040/1‑84 (MQ=255)
                                                                                                   ttGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTc                                                                                                                   <  5:80040/84‑1 (MQ=255)
                                                                                                        gAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGTCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGta                                            <  8:279744/149‑1 (MQ=255)
                                                                                                               cGGTCAGCGCACAGCCAGAGGCCGCCTTGGGGCGGCCCCTGGTTGATGCCCCAGTGTTGTTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGTCCCCACGCCCAGTCCCGAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCaa                                     <  5:137021/149‑1 (MQ=255)
                                                                                                                  tCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACTGTAGCGGCCATGATTGCCACCAGTACTACCAAGcc                                  <  7:331058/149‑1 (MQ=255)
                                                                                                                    aGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGccc                                 <  1:206857/148‑1 (MQ=255)
                                                                                                                    aGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCa                                >  4:231821/1‑149 (MQ=255)
                                                                                                                           aGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAg                         >  6:190819/1‑149 (MQ=255)
                                                                                                                           aGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAg                         <  7:154603/149‑1 (MQ=255)
                                                                                                                                  ggCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCGTGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAg                  >  7:205823/1‑149 (MQ=255)
                                                                                                                                     cgccTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTg               >  5:287585/1‑149 (MQ=255)
                                                                                                                                      gccTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTgg              <  6:346420/149‑1 (MQ=255)
                                                                                                                                       ccTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGga             <  4:94214/149‑1 (MQ=255)
                                                                                                                                            cggcggCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACa        <  5:190819/149‑1 (MQ=255)
                                                                                                                                              gcggcCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTACCCAGATAATCACGGTAGCGGCCATGATTGCCCCCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAg       >  6:336784/1‑148 (MQ=255)
                                                                                                                                                ggcCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGaa    <  7:103791/149‑1 (MQ=255)
                                                                                                                                                   cccTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGAGCGGGCCCCCCGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCACCCAGTACTACCAAGCCCACTGCCAGCCCCGAGCTGGAGAACAGGAAGc  >  1:84190/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTCGGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGA‑CGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGC  >  NC_002947/1835508‑1835802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑