Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,853,215 T→C 7.2% intergenic (+60/‑64) relA → / → mazG ATP:GTP 3'‑pyrophosphotransferase/modified nucleoside triphosphate pyrophosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,853,2150TC 7.2% 157.1 / 2.1 55intergenic (+60/‑64)relA/mazGATP:GTP 3'‑pyrophosphotransferase/modified nucleoside triphosphate pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base T (26/25);  new base C (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGACGCGCTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACGGCTGCCCGTGGGACCTAAA  >  NC_002947/1853076‑1853358
                                                                                                                                           |                                                                                                                                               
tGGACGCGCTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCg                                                                                                                                         >  6:360884/1‑148 (MQ=255)
     gcgcTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCtg                                                                                                                                   >  4:172416/1‑149 (MQ=255)
      cgcTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCtgt                                                                                                                                  <  3:172416/149‑1 (MQ=255)
       gcTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCtgtg                                                                                                                                 >  8:200695/1‑149 (MQ=255)
       gcTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCATAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAACCCCGCTCCTACAGGATGGATGCAGGGTTGAAATCTGCACCGTACCtgtg                                                                                                                                 <  1:286554/149‑1 (MQ=255)
                         gCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCcgcg                                                                                                               <  7:200695/149‑1 (MQ=255)
                               ctcGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGaagaag                                                                                                         >  5:350918/1‑149 (MQ=255)
                                  gCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAATTCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGc                                                                                                        <  7:188828/147‑1 (MQ=255)
                                              catcatCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGAc                                                                                          >  1:234821/1‑149 (MQ=255)
                                               atcatcGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGAcc                                                                                         >  2:18404/1‑149 (MQ=255)
                                                tcatcGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCCACAGGTACGGTGCAGATTTCAAACCTGCATCCATCCTGTAGGAGCgg                                                                                                                          >  8:290214/1‑115 (MQ=17)
                                                tcatcGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCCACAGGTACGGTGCAGATTTCAAACCTGCATCCATCCTGTAGGAGCgg                                                                                                                          <  7:290214/115‑1 (MQ=17)
                                                  atcGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGAcctgc                                                                                      >  8:236215/1‑149 (MQ=255)
                                                   tcGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGAcctgcc                                                                                     >  5:321403/1‑149 (MQ=255)
                                                     gagaCGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGAcctgcctg                                                                                   >  7:167563/1‑149 (MQ=255)
                                                     gagaCGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGAcctgcctg                                                                                   >  3:179155/1‑149 (MQ=255)
                                                     gagaCGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGAcctgcctg                                                                                   >  1:16238/1‑149 (MQ=255)
                                                     gagaCGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGAcctgcct                                                                                    <  1:18404/148‑1 (MQ=255)
                                                       gaCGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTa                                                                                 <  5:105454/149‑1 (MQ=255)
                                                          gcggcgTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCcgc                                                                                                                >  8:335602/1‑115 (MQ=255)
                                                          gcggcgTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCcgc                                                                                                                <  7:335602/115‑1 (MQ=255)
                                                                 aaTCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTacac                                                                       >  5:231992/1‑149 (MQ=255)
                                                                  aTCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACAcc                                                                      >  6:119575/1‑149 (MQ=255)
                                                                      tACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCtt                                                                   >  3:5915/1‑148 (MQ=255)
                                                                        ccccTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTgac                                                                <  6:321403/149‑1 (MQ=255)
                                                                           cTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTgacgac                                                             >  2:334473/1‑149 (MQ=255)
                                                                                  ccGGGGCGTCTGCTTCGCTGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCtgctgc                                                      <  7:236215/149‑1 (MQ=255)
                                                                                    ggggCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCAc                                                    >  4:174330/1‑149 (MQ=255)
                                                                                        cGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCa                                                >  5:350902/1‑149 (MQ=255)
                                                                                          tCTGCTTCGCGGGTAAACCCGCTCCTACCGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGTCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACATGCTGCACCTCATg                                              <  8:172177/149‑1 (MQ=255)
                                                                                          tCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTgg                                                                                                                                >  4:181842/1‑67 (MQ=255)
                                                                                          tCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTgg                                                                                                                                <  3:181842/67‑1 (MQ=255)
                                                                                              cTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGccc                                          <  6:350918/149‑1 (MQ=255)
                                                                                               ttCGCGGGTAAACCCGCTCCTATAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTCCCCCCTGGACGACCTGCTGCACCTCATGGCCCg                                         >  7:55755/1‑149 (MQ=255)
                                                                                               ttCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCg                                         >  1:158160/1‑149 (MQ=255)
                                                                                                 cgcgGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCg                                         <  5:360884/147‑1 (MQ=255)
                                                                                                            ccGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCAc                                                    <  4:293004/125‑1 (MQ=255)
                                                                                                            ccGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCAc                                                    >  3:293004/1‑125 (MQ=255)
                                                                                                                    aCAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTgacgac                                                             >  3:39476/1‑108 (MQ=255)
                                                                                                                    aCAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTgacgac                                                             <  4:39476/108‑1 (MQ=255)
                                                                                                                     cAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACgg                    <  4:179155/148‑1 (MQ=255)
                                                                                                                     cAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACgg                    <  2:16238/148‑1 (MQ=255)
                                                                                                                        gatggatgCATGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCATGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACGGCt                  <  3:193333/147‑1 (MQ=255)
                                                                                                                        gatggatgCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACGGCTGc                >  7:267204/1‑149 (MQ=255)
                                                                                                                        gatggatgCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACGGCTGc                <  5:119575/149‑1 (MQ=255)
                                                                                                                        gatggatgCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACGGCTGc                <  8:267204/149‑1 (MQ=255)
                                                                                                                        gatggatgCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGAATGAACTACACCCTTGACGACCTGCTGCACCTCATCGCCCGCCTGCTCGACCCTCAGTACGGCTga                >  4:193333/1‑148 (MQ=39)
                                                                                                                         tacggtgCAGATTTCAAACCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGccc                                          >  6:103928/6‑122 (MQ=38)
                                                                                                                         tacggtgCAGATTTCAAACCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGccc                                          <  5:103928/117‑1 (MQ=38)
                                                                                                                            gatgCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACGGCTGCCCGt            <  6:350902/149‑1 (MQ=255)
                                                                                                                                cAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACGGCTGCCCGTggg         <  3:174330/148‑1 (MQ=255)
                                                                                                                                cAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACGGCTGCCCGTGGGa        <  2:234821/149‑1 (MQ=255)
                                                                                                                                 aGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACGGCTGCCCGTGGGAc       <  6:231992/149‑1 (MQ=255)
                                                                                                                                    tttGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACGGCTGCCCGTGGGACCt     >  4:191518/1‑148 (MQ=255)
                                                                                                                                       gAAATCTGCACAGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACGGCTGCCCGTGGGACCTaaa  <  4:5915/148‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                               
TGGACGCGCTGGGGCGCCTGCTGGGGCGGATCTCGCAGTTGCCGAACATCATCGAGACGCGGCGTAATCGTACCCCTTGAGACCGGGGCGTCTGCTTCGCGGGTAAACCCGCTCCTACAGGATGGATGCAGGTTTGAAATCTGCACCGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAAGCCACCACCGAAGGACCTGCCTGTATGACCTACACCCTTGACGACCTGCTGCACCTCATGGCCCGCCTGCGCGACCCGCAGTACGGCTGCCCGTGGGACCTAAA  >  NC_002947/1853076‑1853358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑