Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,874,075 G→A 6.0% R129Q (CGG→CAG)  cobU → nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,874,0750GA 6.0% 196.4 / 2.4 66R129Q (CGG→CAG) cobUnicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (30/32);  new base A (2/2);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

CGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGGCGCACAGCTGTTCATCGGTGGCGAGATGGGCATC  >  NC_002947/1873931‑1874220
                                                                                                                                                |                                                                                                                                                 
cGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTg                                                                                                                                               <  8:109642/149‑1 (MQ=255)
  gTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTggg                                                                                                                                             >  1:181832/1‑149 (MQ=255)
      ccGGGCAGATGCTACGGAACTTCGTCGGCGGGGGCGCGGCGCTCAGTGTGCTGGCGCGGCCGCTGCAGGCCAGCCTGGACGTGGTCGTCCTGGGCCCCATTGACGCGCAGCTGGAACTGCGCGGCGTCCGCCATCTGCGGCTGGGCAcc                                                                                                                                         >  5:271019/1‑149 (MQ=255)
      ccGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCAcc                                                                                                                                         >  5:271014/1‑149 (MQ=255)
      ccGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCAcc                                                                                                                                         >  4:11247/1‑149 (MQ=255)
       cGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCATCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCg                                                                                                                                        <  1:300247/149‑1 (MQ=255)
                  tACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAAc                                                                                                                             >  5:153092/1‑149 (MQ=255)
                   aCGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACt                                                                                                                            >  7:295432/1‑149 (MQ=255)
                    cGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACt                                                                                                                            <  3:11247/148‑1 (MQ=255)
                      cAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTg                                                                                                                         >  2:267063/1‑149 (MQ=255)
                         cTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGccc                                                                                                                      >  8:280990/1‑149 (MQ=255)
                            cgtcgGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGt                                                                                                                    >  1:228354/1‑148 (MQ=255)
                                  cggGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTg                                                                                                             <  6:206348/149‑1 (MQ=255)
                                   gggggCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCA‑CATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGc                                                                                                            >  5:189199/1‑148 (MQ=255)
                                    ggggCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGcc                                                                                                                                >  6:204128/1‑128 (MQ=255)
                                    ggggCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGcc                                                                                                                                <  5:204128/128‑1 (MQ=255)
                                     gggCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCATCTTTGCCCGTCTACCTGCGa                                                                                                          <  4:240971/149‑1 (MQ=255)
                                         gcggcgATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGAc                                                                                                      <  8:56516/149‑1 (MQ=255)
                                         gcggcgATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGAc                                                                                                      >  5:176698/1‑149 (MQ=255)
                                            gcgATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCAGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCg                                                                                                    >  6:277493/1‑148 (MQ=255)
                                            gcgATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCAGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCg                                                                                                    >  6:272495/1‑148 (MQ=255)
                                                tCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCA‑CATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAAcc                                                                                              <  6:189199/149‑1 (MQ=255)
                                                        cTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGc                                                                                        >  7:343164/1‑148 (MQ=255)
                                                            cgcgGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCg                                                                                   <  6:176698/149‑1 (MQ=255)
                                                              cgGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGc                                                                                 >  5:123970/1‑149 (MQ=255)
                                                                   gCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGc                                                                            <  6:153092/149‑1 (MQ=255)
                                                                       cAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGTAAGCGGCCTTGCAGGc                                                                        <  1:274106/149‑1 (MQ=255)
                                                                        aGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCt                                                                       <  8:295432/149‑1 (MQ=255)
                                                                               ccTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACt                                                                                                                                   >  6:196779/1‑82 (MQ=255)
                                                                               ccTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACt                                                                                                                                   <  5:196779/82‑1 (MQ=255)
                                                                                 tGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCTAGCGGCCTTGCAgg                                                                         <  1:99020/138‑1 (MQ=255)
                                                                                 tGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCGAGCGGCCTTGCAgg                                                                         >  2:99020/1‑138 (MQ=255)
                                                                                 tGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGa                                                               >  4:156872/1‑148 (MQ=255)
                                                                                    aCGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGt                                                           <  6:271014/149‑1 (MQ=255)
                                                                                    aCGTGGTCGACCTGGGCACCAGTGACGCGCAGCTGGCACTGCCCGGCGGGCGCCAGCTGCGGCTGGTCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACGAGCTGCAAGCGGCCTTGCAGGCGGGTCGCGACAGt                                                           <  6:271019/149‑1 (MQ=255)
                                                                                         gTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCt                                                                       <  5:341505/132‑1 (MQ=255)
                                                                                         gTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCt                                                                       >  6:341505/1‑132 (MQ=255)
                                                                                         gTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGcc                                                        >  1:306293/1‑147 (MQ=255)
                                                                                         gTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCa                                                      <  7:280990/149‑1 (MQ=255)
                                                                                           cGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCAGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAgc                                                    <  5:272495/149‑1 (MQ=255)
                                                                                            gACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCAGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAgc                                                    <  5:277493/148‑1 (MQ=255)
                                                                                             aCCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAgcgc                                                  <  8:37930/149‑1 (MQ=255)
                                                                                               cTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAgcgcgc                                                >  5:106756/1‑149 (MQ=255)
                                                                                                  ggCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTg                                              <  2:228354/148‑1 (MQ=255)
                                                                                                        aTTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGaa                                         >  2:60528/1‑147 (MQ=255)
                                                                                                            aCGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGgc                                   <  2:77917/149‑1 (MQ=255)
                                                                                                              gcgcAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCg                                           >  3:260193/1‑139 (MQ=255)
                                                                                                              gcgcAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCg                                           <  4:260193/139‑1 (MQ=255)
                                                                                                              gcgcAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGgcg                                  >  8:195472/1‑148 (MQ=255)
                                                                                                                gcAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGGCGca                                >  5:210102/1‑148 (MQ=255)
                                                                                                                    cTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCt                                               <  2:76644/129‑1 (MQ=255)
                                                                                                                    cTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCt                                               >  1:76644/1‑129 (MQ=255)
                                                                                                                    cTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGGCGCACAGCt                           <  8:343164/149‑1 (MQ=255)
                                                                                                                      ggAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGGCGCACAGCTGt                         >  8:230356/1‑149 (MQ=255)
                                                                                                                             cccGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGGCGCACAGCTGTTCATCgg                  >  4:263363/1‑149 (MQ=255)
                                                                                                                             cccGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGGCGCACAGCTGTTCATCgg                  >  8:150547/1‑149 (MQ=255)
                                                                                                                              ccGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGGCGCACAGCTGTTCATCGGt                 >  4:58797/1‑149 (MQ=255)
                                                                                                                                 gCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGGCGCACAGCTGTTCATCGGTGGc              <  1:60528/149‑1 (MQ=255)
                                                                                                                                     gcgcCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGGCGCACAGCTGTTCATCGGTGGc              >  7:15733/1‑145 (MQ=255)
                                                                                                                                      cgcCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGGCGCACAGCTGTTCATCGGTGGCGAGAt         <  1:63322/149‑1 (MQ=255)
                                                                                                                                      cgcCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGGCGCACAGCTGTTCATCGGTGGCGAGAt         <  3:263363/149‑1 (MQ=255)
                                                                                                                                        ccATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGGCGCACAGCTGTTCATCGGTGGCGAGATgg       <  7:230356/149‑1 (MQ=255)
                                                                                                                                        ccATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGGCGCACAGCTGTTCATCGGTGGCGAGATgg       <  7:150547/149‑1 (MQ=255)
                                                                                                                                        ccATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGGCGCACAGCTGTTCATCGGTGGCGAGATgg       <  3:156872/149‑1 (MQ=255)
                                                                                                                                        ccATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGGCGCACAGCTGTTCATCGGTGGCGAGATgg       <  6:210102/149‑1 (MQ=255)
                                                                                                                                               cGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGGCGCACAGCTGTTCATCGGTGGCGAGATGGGCATc  <  7:195472/147‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CGGTAACCGGGCAGATGCTACGCAACTTCGTCGGCGGGGGCGCGGCGATCAGTGTGCTGGCGCGGCAGCTGCAGGCCAGCCTGGACGTGGTCGACCTGGGCACCATTGACGCGCAGCTGGAACTGCCCGGCGTGCGCCATCTGCGGCTGGGCACCGGTACTGCCAACTTTGCCCGTCAACCTGCGATGACCGAAAACCAGCTGCAAGCGGCCTTGCAGGCTGGTCGCGACAGTGCCCAGCGCGCTGCCGAACAGGGCGCACAGCTGTTCATCGGTGGCGAGATGGGCATC  >  NC_002947/1873931‑1874220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑