Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,887,064 T→C 6.7% Q1070R (CAG→CGG)  PP_1695 ← sodium‑solute symporter/two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,887,0640TC 6.7% 173.6 / 2.3 60Q1070R (CAG→CGG) PP_1695sodium‑solute symporter/two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base T (29/26);  new base C (2/2);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

ATCACTACACCGGGTACCGGCTCACCCAGCCGTGCTCGAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACTTGCAGCCCGGCCAGTGGCTGGCCGCCCTGTTGTTCCACGGGCGTGCTTGGCGCCG  >  NC_002947/1886920‑1887211
                                                                                                                                                |                                                                                                                                                   
aTCACTACACCGGGTACCGGCTCACCCAGCCGTGCTCGAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGtt                                                                                                                                                 >  5:198646/1‑149 (MQ=255)
 tCACTACACCGGGTACCGGCTCACCCAGCCGTGCTCGAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTg                                                                                                                                                >  1:12276/1‑149 (MQ=255)
  cACTACACCGGGTACCGGCTCACCCAGCCGTGCTCGAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCCGGTTgc                                                                                                                                               >  1:284403/1‑149 (MQ=255)
  cACTACACCGGGTACCGGCTCACCCAGCCGTGCTCGAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCCGGTTgc                                                                                                                                               >  5:125288/1‑149 (MQ=255)
      acacCGGGTACCGGCTCACCCAGCCGTGCTCGAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTgcgcgc                                                                                                                                           >  3:103329/1‑149 (MQ=255)
       cacCGGGTACCGGCTCACCCAGCCGTGCTCGAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGcgcgcg                                                                                                                                          <  5:171363/149‑1 (MQ=255)
        acCGGGTACCGGCTCACCCAGCCGTGCTCGAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCgg                                                                                                                                         >  7:92271/1‑149 (MQ=255)
                 cGGCTCACCCAGCCGTGCTCGAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCt                                                                                                                                >  2:354658/1‑149 (MQ=255)
                  ggCTCTCCCTGCCGTGCTCGAAGGCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTACAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCAAACCTg                                                                                                                               <  8:77848/149‑1 (MQ=255)
                  ggCTCACCCAGCCGTGCTCGAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTg                                                                                                                               <  5:152346/149‑1 (MQ=255)
                   gCTCACCCAGCCGTGCTCGAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTgg                                                                                                                              <  8:92271/149‑1 (MQ=255)
                       aCCCAGCCGTGCTCGAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCaa                                                                                                                           <  2:209380/148‑1 (MQ=255)
                         ccAGCCGTGCTCGAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTgcgcg                                                                                                                                            <  3:215255/129‑1 (MQ=255)
                         ccAGCCGTGCTCGAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAAccc                                                                                                                        >  6:171879/1‑149 (MQ=255)
                         ccAGCCGTGCTCGAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGAGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCAGTCGGCCTGGTTgcgag                                                                                                                                            >  4:215255/1‑127 (MQ=255)
                           aGCCGTGCTCGAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGACAGCAGGGCTTCGCATTCGGCATCGTTGCGCGCGGTCCATACCTGGCAAacc                                                                                                                        >  6:83281/1‑147 (MQ=255)
                                   tCGAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCt                                                                                                                >  5:255843/1‑147 (MQ=255)
                                     gAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCAGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTcatt                                                                                                            >  5:315208/1‑147 (MQ=255)
                                       agccagccCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTcagcag                                                                                                          >  8:317401/1‑149 (MQ=255)
                                        gccagccCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAgg                                                                                                         <  7:124059/149‑1 (MQ=255)
                                           agccCATCAGCCCGGTGCCGGGCTCGCGGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCGTTTGGCCAGCAGGGCTTGGCTTTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTg                                                                                                      <  5:83281/149‑1 (MQ=255)
                                             cgcATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCAGACAGGGCAACCCCAGCGGCTCCGCAGGCTGtt                                                                                                    >  4:219054/3‑149 (MQ=255)
                                                aTCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCAt                                                                                                 <  7:317401/149‑1 (MQ=255)
                                                aTCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCAt                                                                                                 <  1:354658/149‑1 (MQ=255)
                                                    gCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGcc                                                                                              >  5:121284/1‑148 (MQ=255)
                                                         gTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCACATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAg                                                                                        >  7:47849/1‑149 (MQ=255)
                                                               gTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCt                                                                                  <  6:198646/149‑1 (MQ=255)
                                                                  tcGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTcagc                                                                                                            <  4:95426/120‑1 (MQ=255)
                                                                  tcGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTcagc                                                                                                            >  3:95426/1‑120 (MQ=255)
                                                                    gCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCt                                                                              <  5:171879/148‑1 (MQ=255)
                                                                      cgtcgtCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCg                                                                           <  4:103329/149‑1 (MQ=255)
                                                                      cgtcgtCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCg                                                                           <  6:121284/149‑1 (MQ=255)
                                                                       gtcgtcCAGGTGGTAGTCCACCAATGCGATGTGCGGGCGCATGCCTTTGGCAAGCAGGGCTTCGCATTCGGCCTGGTTGCTCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGt                                                                          <  3:220399/149‑1 (MQ=255)
                                                                        tcgtcCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGtt                                                                         <  3:273472/149‑1 (MQ=255)
                                                                           tcCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCCGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTc                                                                      <  2:284403/149‑1 (MQ=255)
                                                                           tcCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCCGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTc                                                                      <  6:125288/149‑1 (MQ=255)
                                                                             cAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCt                                                                                  <  4:266787/135‑1 (MQ=255)
                                                                             cAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCt                                                                                  >  3:266787/1‑135 (MQ=255)
                                                                                gtggtAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTc                                                                            >  8:4800/1‑138 (MQ=255)
                                                                                gtggtAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTc                                                                            <  7:4800/138‑1 (MQ=255)
                                                                                         caccaATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGcc                                                                                              >  1:76109/1‑111 (MQ=255)
                                                                                         caccaATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACtt                                                        >  3:306939/1‑149 (MQ=255)
                                                                                         caccaATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACt                                                         <  6:234228/148‑1 (MQ=255)
                                                                                          accaATGCCAGGTGCGGGCGCATGCCTTTTGCCAGCAGGGCTTCGCATTAGGCCTGGTTGCGCGCTGTCCATACCTGGCAACCCCAGCTGCTCAGCATGCTGTTCATGCCGATCATGATGCTGTCTTCGTTGTCCACGCATAGCACtt                                                        >  4:355242/1‑148 (MQ=255)
                                                                                          accaATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACTTg                                                       >  4:349661/1‑149 (MQ=255)
                                                                                           ccaATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACTTg                                                       >  3:227652/1‑148 (MQ=255)
                                                                                                         gggCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACTTGCAGCCCGGCCAGtgg                                        <  3:219054/149‑1 (MQ=255)
                                                                                                           gcgcATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACTTGCAGCCCGGCCAGtggct                                      >  7:172877/1‑149 (MQ=255)
                                                                                                                tGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACTTGCAGCCCGGCCAGTGGCTGgccg                                 >  2:147435/1‑149 (MQ=255)
                                                                                                                         ccAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACTTGCAGCCCGGCCAGTGGCTGgcc                                  >  2:329891/1‑139 (MQ=255)
                                                                                                                         ccAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACTTGCAGCCCGGCCAGTGGCTGgcc                                  <  1:329891/139‑1 (MQ=255)
                                                                                                                          cagctgGGATTCGCATTCGGCCTGGTTTCGCGCGGTCCATTCCTGGCAACCCCAGCGGCTCAGCAGGATGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATATCACTTGCAGCCCTGCCAGtggat                                      <  6:282025/134‑3 (MQ=255)
                                                                                                                          cagcagGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACTTGCAGCCCGGCCAGTGGCTGGCCGCCCTgttgtt                       <  4:227652/149‑1 (MQ=255)
                                                                                                                          cagcagGGCTTCGCATTCGGCCTGGTTGCGAGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACTTGCAGCCCGGCCAGtggct                                      >  5:282025/1‑134 (MQ=255)
                                                                                                                                  cTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACTTGCAGCCCGGCCAGTGGCTGGCCGccc                              >  2:267703/1‑134 (MQ=255)
                                                                                                                                  cTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACTTGCAGCCCGGCCAGTGGCTGGCCGccc                              <  1:267703/134‑1 (MQ=255)
                                                                                                                                   ttCGCATTCGGCCTGGTTGCGCGCGGTCCTTACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACTTGCAGCCCGGCCAGTGGCTGGCCGCCCTGTTGTTCCACGGGCg              >  7:165601/1‑149 (MQ=255)
                                                                                                                                        aTTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACTTGCAGCCCGGCCAGTGGCTGGCCGCCCTGTTGTTCCACGGGCGTGCtt         <  8:172877/149‑1 (MQ=255)
                                                                                                                                          tCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACTTGCAGCCCGGCCAGTGGCTGGCCGCCCTGTTGTTCCACGGGCGTGCTTgg       >  5:34682/1‑149 (MQ=255)
                                                                                                                                               cTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACTTGCAGCCCGGCCAGTGGCTGGCCGCCCTGTTGTTCCACGGGCGTGCTTGGcgccg  >  3:125610/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
ATCACTACACCGGGTACCGGCTCACCCAGCCGTGCTCGAAGCCAGCCCATCAGCCCGGTGCCGGTCTCGCCGTCGTCCAGGTGGTAGTCCACCAATGCCAGGTGCGGGCGCATGCCTTTGGCCAGCAGGGCTTCGCATTCGGCCTGGTTGCGCGCGGTCCATACCTGGCAACCCCAGCGGCTCAGCAGGCTGTTCATGCCGATCAGGATGCTGTCTTCGTTGTCCACGCATAGCACTTGCAGCCCGGCCAGTGGCTGGCCGCCCTGTTGTTCCACGGGCGTGCTTGGCGCCG  >  NC_002947/1886920‑1887211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑