Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,925,860 C→T 7.4% A336T (GCC→ACC)  PP_1726 ← ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,925,8600CT 7.4% 154.9 / 2.1 54A336T (GCC→ACC) PP_1726ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (25/25);  new base T (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.68e-01

ACCCGCTCCTACATGTATCGCGTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGCCTGCACATCCGCCGGCAGCTTCAGGTGCTCGGCCCGGATCGGCCGCGCATGGCCCTTGGCCAGGTTGATCTGCCCGGCGTC  >  NC_002947/1925714‑1926007
                                                                                                                                                  |                                                                                                                                                   
aCCCGCTCCTACATGTATCGCGTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGTTGGCtt                                                                                                                                                   <  8:41276/149‑1 (MQ=255)
     cTCCTACATGTATCGCGTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAg                                                                                                                                              >  5:51876/1‑149 (MQ=255)
        cTACATGTATCGCGTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGGCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCt                                                                                                                                           >  7:269162/1‑149 (MQ=255)
        cTACATGTATCGCGTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCt                                                                                                                                           >  3:8361/1‑149 (MQ=255)
         tACATGTATCGCGTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGTTTCCCAGGCtt                                                                                                                                          >  3:227643/1‑149 (MQ=255)
          aCATGTATCGCGTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGTTTCCCAGGCTTc                                                                                                                                         >  5:237800/1‑149 (MQ=255)
          aCATGTATCGCGTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTc                                                                                                                                         >  5:249838/1‑149 (MQ=255)
          aCATGTATCGCGTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTc                                                                                                                                         >  2:351167/1‑149 (MQ=255)
           cATGTATCGCGTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCg                                                                                                                                        >  6:69404/1‑149 (MQ=255)
            aTGTATCGCGTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACATGCTCCTGCCACATATGCGGCAGTTTATTCGAGGTGGCTTCCCAGGATTCgg                                                                                                                                       >  4:107185/1‑149 (MQ=255)
              gTATCGCGTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCtt                                                                                                                                          <  8:190084/144‑1 (MQ=255)
              gTATCGCGTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCtt                                                                                                                                          >  7:190084/1‑144 (MQ=255)
                 tCGCGTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGTTTCCCAGGCTTCGGCATTc                                                                                                                                  <  6:237800/149‑1 (MQ=255)
                 tCGCGTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTc                                                                                                                                  <  1:351167/149‑1 (MQ=255)
                 tCGCGTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTc                                                                                                                                  <  5:325739/149‑1 (MQ=255)
                 gcgcgTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTc                                                                                                                                  <  3:151156/148‑1 (MQ=255)
                   gcgTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGGCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCtt                                                                                                                                <  8:269162/149‑1 (MQ=255)
                   gcgTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCtt                                                                                                                                >  4:338990/1‑149 (MQ=255)
                   gcgTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCtt                                                                                                                                >  3:234786/1‑149 (MQ=255)
                        tCCCTGTTTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATc                                                                                                                            <  3:107185/148‑1 (MQ=255)
                        tCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGTTTCCCAGGCTTCGGCATTCTTGATCg                                                                                                                           <  4:227643/149‑1 (MQ=255)
                        tCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATc                                                                                                                            <  6:51876/148‑1 (MQ=255)
                          ccTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGc                                                                                                                         >  5:46623/1‑149 (MQ=255)
                           cTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCt                                                                                                                        >  8:274648/1‑149 (MQ=255)
                           cTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCt                                                                                                                        <  2:55411/149‑1 (MQ=255)
                                                    aaaGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTggg                                                                                               <  6:46623/149‑1 (MQ=255)
                                                       gggCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGgcag                                                                                            <  7:51716/149‑1 (MQ=255)
                                                              aTCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTg                                                                                     <  4:8361/149‑1 (MQ=255)
                                                                    cACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGCCTGc                                                                               <  5:69404/149‑1 (MQ=255)
                                                                                cagcccCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGgcagc                                                                                           <  5:214444/125‑1 (MQ=255)
                                                                                cagcccCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGgcagc                                                                                           >  6:214444/1‑125 (MQ=255)
                                                                                cagcccCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGCCTGCACATCCGCCGGc                                                                   <  6:249838/149‑1 (MQ=255)
                                                                                 agcccCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTc                                                                                                                                         >  7:228761/1‑78 (MQ=255)
                                                                                 agcccCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTc                                                                                                                                         <  8:228761/78‑1 (MQ=255)
                                                                                       ttACTCCATCTCGATGCTCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCtt                                                                                      <  6:270064/123‑1 (MQ=255)
                                                                                       ttACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCGCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGGAGCTTGGCGTGCACATCCGCCGGCAGCtt                                                              >  6:368211/1‑147 (MQ=255)
                                                                                                  cGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGCCTGCACATCCGCCGGCAGCTTCAGGTGCTCGGcc                                                 >  8:132181/1‑149 (MQ=255)
                                                                                                        tCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTg                                                                                                 >  2:954/1‑95 (MQ=255)
                                                                                                        tCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGCCTGCACATCCGCCGGCAGCTTCAGGTGCTCGGCCCGGATc                                           >  5:224316/1‑149 (MQ=255)
                                                                                                            cTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCATGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGCCTGCACATCCGCCGGCAGCTTCAg                                                           <  3:145508/129‑1 (MQ=255)
                                                                                                            cTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCATGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGCCTGCACATCCGCCGGCAGCTTCAg                                                           >  4:145508/1‑129 (MQ=255)
                                                                                                            cTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGc                                                                                   <  6:294101/105‑1 (MQ=255)
                                                                                                            cTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGc                                                                                   >  5:294101/1‑105 (MQ=255)
                                                                                                                     ccACATCTGCGGCAGTTTCTTCGAGGTCGCGTCCCAGGCTTCGGCATTCATGATCGGCTGGGCGGCAGCGGACTGGTCGGTGGGCAGCAGCTTGGCCTGCACATCCGCCGGCAGCTTCAGGTGCTCGGCCCGGATCGGCCGCGCATGGc                              <  5:368211/149‑1 (MQ=255)
                                                                                                                            tGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGCCTGCACATCCGCCGGCAGCTTCAGGTGCTCGGCCCGGATCGGCCGCGCATGGCCCttc                         >  5:159488/1‑146 (MQ=255)
                                                                                                                            tGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGCCTGCACATCCGCCGGCAGCTTCAGGTGCTCGGCCCGGATCGGCCGCGCATGGCCCTTg                         <  6:159488/147‑1 (MQ=255)
                                                                                                                                  aGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGCCTGCACATCCGCCGGCAGCTTCAGGTGCTCGGCCCGGATCg                                          >  2:248165/1‑124 (MQ=255)
                                                                                                                                  aGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGCCTGCACATCCGCCGGCAGCTTCAGGTGCTCGGCCCGGATCg                                          <  1:248165/124‑1 (MQ=255)
                                                                                                                                        ttcGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGCCTGCACATCCGCCGGCAGCTTCAGGTGCTCGGCCCGGATCGGCCGCGCATGGCCCTTGGCCAGGTTGATCTg           >  4:31511/1‑149 (MQ=255)
                                                                                                                                              gTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGCCTGCACATCCGCCGGCAGCTTCAGGTGCTCGGCCCGGATCGGCCGCGCATGGCCCTTGGCCAGGt                  <  3:40239/136‑1 (MQ=255)
                                                                                                                                              gTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGCCTGCACATCCGCCGGCAGCTTCAGGTGCTCGGCCCGGATCGGCCGCGCATGGCCCTTGGCCAGGt                  <  3:35636/136‑1 (MQ=255)
                                                                                                                                              gTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGCCTGCACATCCGCCGGCAGCTTCAGGTGCTCGGCCCGGATCGGCCGCGCATGGCCCTTGGCCAGGt                  >  4:40239/1‑136 (MQ=255)
                                                                                                                                              gTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGCCTGCACATCCGCCGGCAGCTTCAGGTGCTCGGCCCGGATCGGCCGCGCATGGCCCTTGGCCAGGt                  >  4:35636/1‑136 (MQ=255)
                                                                                                                                                 gCTTCCCAGGCTTCGGCATTCTTGCTCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGCCTGCACATCCGCCGGCAGCTTCAGGTGCTCGGCCCGGATCGGCCGCGCATGGCCCTTGGCCAGGTTGATCTGCCCGGCGTc  <  4:234786/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ACCCGCTCCTACATGTATCGCGTATCCCTGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGATCAGGCACTACAAAGCCCAGCCCCTTACTCCATCTCGATGATCACCTGCTCCTGCCACATCTGCGGCAGTTTCTTCGAGGTGGCTTCCCAGGCTTCGGCATTCTTGATCGGCTGGGCGGCAGCGTACTGGTCGTTGGGCAGCAGCTTGGCCTGCACATCCGCCGGCAGCTTCAGGTGCTCGGCCCGGATCGGCCGCGCATGGCCCTTGGCCAGGTTGATCTGCCCGGCGTC  >  NC_002947/1925714‑1926007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑