Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,935,861 A→G 7.3% T55A (ACC→GCC)  PP_1736 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,935,8610AG 7.3% 155.0 / 2.5 55T55A (ACC→GCC) PP_1736hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (22/29);  new base G (2/2);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

ACCGGTCTCATCCTGTCTGGCGGCGGCGCCCGCGCCGCCTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAGCGACTGGCCCGGGGTCGTGCGCCAGGCCAGCCGTTTCGTTGGCCACAGCCTGCT  >  NC_002947/1935717‑1936006
                                                                                                                                                |                                                                                                                                                 
aCCGGTCTCATCCTGTCTGGCGGCGGCGCCCGCGCCGCCTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCAccc                                                                                                                                                <  3:257444/148‑1 (MQ=255)
aCCGGTCTCATCCTGTCTGGCGGCGGCGCCCGCGCCGCCTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCt                                                                                                                                               <  8:76658/149‑1 (MQ=255)
 ccGGTCTCATCCTGTCTGGCGGCGGCGCCCGCGCCGCCTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTg                                                                                                                                              >  7:36920/1‑149 (MQ=255)
     tctcATCCTGTCTGGCGGCGGCGCCCGCGCCGCCTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCa                                                                                                                                          <  8:36920/149‑1 (MQ=255)
       tcATCCTGTCTGGCGGCGGCGCCCGCGCCGCCTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAgt                                                                                                                                        >  7:145570/1‑149 (MQ=255)
         aTCCTGTCTGGCGGCGGCGCCCGCGCCGCCTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGc                                                                                                                                            <  5:140191/143‑1 (MQ=255)
         aTCCTGTCTGGCGGCGGCGCCCGCGCCGCCTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAgtgg                                                                                                                                      >  8:276499/1‑149 (MQ=255)
         aTCCTGTCTGGCGGCGGCGCCCGCGCCGCCTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAgtg                                                                                                                                       <  5:208212/148‑1 (MQ=255)
         aTCCTGTCTGGCGGCGGCGCCCGCGCCGCCTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAgtg                                                                                                                                       <  3:96206/148‑1 (MQ=255)
          tCCTGTCTGGCGGCGGCGCCCGCGCCGCCTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAgtgg                                                                                                                                      <  5:199876/148‑1 (MQ=255)
          tCCTGTCTGGCGGCGGCGCCCGCGCCGCCTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAgtgg                                                                                                                                      >  6:199876/1‑148 (MQ=255)
               tCTGGCGGCGGCGCCCGCGCCGCCTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTggtgcggt                                                                                                                                <  8:241477/149‑1 (MQ=255)
               tCTGGCGGCGGCGCCCGCGCCGCCTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTggtgcgg                                                                                                                                 <  3:90663/148‑1 (MQ=255)
                 tGGCGGCGGCGCCCGCGCCGCCTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTggtgcggtg                                                                                                                               >  6:357766/1‑148 (MQ=255)
                   gcggcggcgCCCGCGCCGCCTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTggtgcggtg                                                                                                                               <  7:276499/146‑1 (MQ=255)
                         gcgcCCGCGCCGCCTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAAt                                                                                                                      <  5:357766/149‑1 (MQ=255)
                                  ccgccTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTg                                                                                                             <  5:180999/149‑1 (MQ=255)
                                    gccTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGAc                                                                                                                   <  6:216845/141‑1 (MQ=255)
                                    gccTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGAc                                                                                                                   >  5:216845/1‑141 (MQ=255)
                                       tACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCg                                                                                                        >  6:299727/1‑149 (MQ=255)
                                         ccAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTg                                                                                                             >  1:28639/1‑142 (MQ=255)
                                         ccAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTg                                                                                                             <  2:28639/142‑1 (MQ=255)
                                         ccAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCg                                                                                                        <  8:145570/147‑1 (MQ=255)
                                            ggTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCa                                                                                                   <  2:128759/149‑1 (MQ=255)
                                             gTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCAc                                                                                                  <  7:299589/149‑1 (MQ=255)
                                                     gCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGa                                                                                                                    <  7:305985/123‑1 (MQ=255)
                                                     gCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGa                                                                                                                    >  8:305985/1‑123 (MQ=255)
                                                              cATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCgg                                                                                                       >  8:26051/1‑127 (MQ=255)
                                                              cATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCgg                                                                                                       <  7:26051/127‑1 (MQ=255)
                                                                tCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTc                                                                               <  5:292312/149‑1 (MQ=255)
                                                                  gCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCg                                                                             <  5:299727/149‑1 (MQ=255)
                                                                           ctgcCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCt                                                                    <  6:34140/149‑1 (MQ=255)
                                                                              cccGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGt                                                                 <  7:207759/149‑1 (MQ=255)
                                                                                cgccgGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCa                                                               >  1:323595/1‑149 (MQ=255)
                                                                                    ggCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCg                                                           <  5:148496/149‑1 (MQ=255)
                                                                                     gcgcGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCg                                                           >  6:4396/1‑148 (MQ=255)
                                                                                       gcgGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAg                                                        >  3:301103/1‑149 (MQ=255)
                                                                                               cccTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCa                                                                           >  5:235977/1‑122 (MQ=255)
                                                                                               cccTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCa                                                                           <  6:235977/122‑1 (MQ=255)
                                                                                               cccTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAGCGACTGGc                                                >  4:197603/1‑149 (MQ=255)
                                                                                                      ccGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCGCCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAGCGACTGGCCCGGGGt                                         >  1:282098/1‑149 (MQ=255)
                                                                                                      ccGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCGCCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAGCGACTGGCCCGGGGt                                         >  1:298771/1‑149 (MQ=255)
                                                                                                       cGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAGCGACTGGCCCGGGGTc                                        >  7:142807/1‑149 (MQ=255)
                                                                                                                       cTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAGCGACTGGCCCGGGGTCGTGCGCCAGGCCAGcc                        >  3:71778/1‑149 (MQ=255)
                                                                                                                            ccgGGGCCATCAACGCCGTCGCCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAGCGACTGGCCCGGGGTCGTGCGCCAGGCCAGCCGTTTc                   <  2:282098/149‑1 (MQ=255)
                                                                                                                            ccgGGGCCATCAACGCCGTCGCCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAGCGACTGGCCCGGGGTCGTGCGCCAGGCCAGCCGTTTc                   <  2:298771/149‑1 (MQ=255)
                                                                                                                                 gCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAGCGACTGGCCCGGGGTCGTGCGCCAGGCCAGCCGTTTCGTTgg              >  3:217084/1‑149 (MQ=255)
                                                                                                                                     tCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAGCGACTGGCCCGGGGTCGTGCGCCAGGCCAGCCGTTTCGTTGGCcac          <  4:217084/149‑1 (MQ=255)
                                                                                                                                     tCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGACGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAg                                                        <  8:97194/103‑1 (MQ=255)
                                                                                                                                     tCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGACGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAg                                                        >  7:97194/1‑103 (MQ=255)
                                                                                                                                      cAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAGCGACTGGCCCgggg                                          <  6:318565/116‑1 (MQ=255)
                                                                                                                                      cAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAGCGACTGGCCCgggg                                          >  5:318565/1‑116 (MQ=255)
                                                                                                                                        aCGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAGCGACTGGCCCGGGGTCGTGCGCCAGGCCAGCCGTTTCGTTGGCCACAGc       <  5:4396/149‑1 (MQ=255)
                                                                                                                                          gCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAGCGACTGGCCCGGGGTCGTGCGCCAGGCCAGCCGTTTCGTTGGCCACAGCct     >  3:37303/1‑149 (MQ=255)
                                                                                                                                             gTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAGCGACTGGCCCGGGGTCGTGCGCCAGGCCAGCCGTTTCGTTGGCCACAGCctgct  >  3:291340/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
ACCGGTCTCATCCTGTCTGGCGGCGGCGCCCGCGCCGCCTACCAGGTCGGTGTGCTGGCGGGCATCGCCGAGTTGCTGCCCGCCGGCGCGGACAACCCTTTTCCGGTCATCGTCGGCACCTCCGCCGGGGCCATCAACGCCGTCACCCTGGCCAGTGGTGCGGTGCACTTCAATGACGCCGTGCAGCGGCTCACTGCCTTCTGGCAGAATTTCCGCAGCCATCTGGTCATCCGCAGCGACTGGCCCGGGGTCGTGCGCCAGGCCAGCCGTTTCGTTGGCCACAGCCTGCT  >  NC_002947/1935717‑1936006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑