Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,976,785 T→G 8.0% F150C (TTC→TGC)  PP_1770 → 3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,976,7850TG 8.0% 148.8 / 4.2 50F150C (TTC→TGC) PP_17703‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base T (20/26);  new base G (2/2);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

GCAATCATCACCGACGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGCCACCAGC  >  NC_002947/1976637‑1976930
                                                                                                                                                    |                                                                                                                                                  
gCAATCATCACCGACGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGtt                                                                                                                                                    >  2:116504/1‑149 (MQ=255)
  aaTCATCACCGACGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTcc                                                                                                                                                  >  6:184925/1‑149 (MQ=255)
    tcatcaCCGACGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGTGTAGAGGCGTCCAATGCCACCTTGTTccgc                                                                                                                                                <  8:5735/149‑1 (MQ=255)
       tcaCCGACGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCgg                                                                                                                                             >  2:253366/1‑149 (MQ=255)
          ccGACGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGcac                                                                                                                                          <  4:142955/149‑1 (MQ=255)
             aCGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAg                                                                                                                                       <  1:253366/149‑1 (MQ=255)
             aCGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAg                                                                                                                                       <  8:95534/149‑1 (MQ=255)
             aCGGCGGCAGCGCCACGGGCAACGTCGGGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGGTTCGTCCCGGGCCACCCCATCGCCGGTTCCGGGCAGAGCGGGGTAGAGGCGTCCAATGGCACCTTGTTCCGCCGGCACAAg                                                                                                                                       <  4:252441/149‑1 (MQ=255)
                tCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTc                                                                                                                                    <  7:84775/149‑1 (MQ=255)
                tCGGCAGCGCCAAGGGCAACGTCGTGCGGGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTGCCGGCGGAACAAGGtg                                                                                                                                    <  3:271607/149‑2 (MQ=255)
                   gCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATc                                                                                                                                 <  4:77391/149‑1 (MQ=255)
                     aGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCt                                                                                                                               <  5:184925/149‑1 (MQ=255)
                                 aaCGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGc                                                                                                                   >  5:124304/1‑149 (MQ=255)
                                       gtgcgtgAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAAc                                                                                                             <  6:292317/149‑1 (MQ=255)
                                         gcgtgAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCCTCGCCGGTTCCGAGCAGAGCGGGGTAAAGGCGTCCAATGCCACCATGTTCCGCCGGGACAAGGTCATCCACCCCCCGCTGGCCGAAACCg                                                                                                           >  4:151193/1‑149 (MQ=255)
                                         gcgtgAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCAcg                                                                                                                           <  6:108871/133‑1 (MQ=255)
                                         gcgtgAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCAcg                                                                                                                           >  5:108871/1‑133 (MQ=255)
                                                        cGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGca                                                                                                                                           >  7:196807/1‑102 (MQ=255)
                                                        cGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGca                                                                                                                                           <  8:196807/102‑1 (MQ=255)
                                                        cGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTgg                                                                                            <  1:254122/149‑1 (MQ=255)
                                                         gTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGt                                                                                           >  4:161759/1‑149 (MQ=255)
                                                               ggTGCCCGCCTGGCGCGCTTCGTCCCGGTCCACCGCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCTCCGGCACAAGGTCATCCTCACGCCGCTTGCCGAAACCGACCCCCGCGCGCTGGTCCTgg                                                                                      <  8:162752/148‑1 (MQ=255)
                                                               ggTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGt                                                                                     >  8:276233/1‑149 (MQ=255)
                                                                   cccGCCTGCCGCGCTTCGTCCCGGGCCACACCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCATGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGAc                                                                                 >  5:278229/1‑149 (MQ=255)
                                                                    ccGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGAcc                                                                                >  2:286203/1‑149 (MQ=255)
                                                                           ccGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCtg                                                                           <  3:161759/147‑1 (MQ=255)
                                                                                     tCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTg                                                              >  6:221441/1‑149 (MQ=255)
                                                                                         gggCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTgc                                                                  <  5:192132/141‑1 (MQ=255)
                                                                                         gggCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTgc                                                                  >  6:192132/1‑141 (MQ=255)
                                                                                             cACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGa                                                      <  6:124304/149‑1 (MQ=255)
                                                                                               ccccATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGAtg                                                    >  6:368276/1‑149 (MQ=255)
                                                                                                             ccGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCTGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGAtgtg                                                  <  2:337377/137‑1 (MQ=255)
                                                                                                             ccGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGAtgtg                                                  >  1:337377/1‑137 (MQ=255)
                                                                                                                    gagCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAgc                               <  1:188218/149‑1 (MQ=255)
                                                                                                                    gagCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGGTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAgc                               <  5:221441/149‑1 (MQ=255)
                                                                                                                    gagCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCACGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAgc                               >  5:185600/1‑149 (MQ=255)
                                                                                                                       cGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGCTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAgcgc                             <  1:256694/149‑1 (MQ=255)
                                                                                                                            tAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCACGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGAc                       <  6:185600/149‑1 (MQ=255)
                                                                                                                            gcgagGCGTCCAATGCCTCCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCTTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGAc                       <  6:232109/147‑1 (MQ=255)
                                                                                                                                   gTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCAGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTAc                >  8:39774/1‑149 (MQ=255)
                                                                                                                                      cAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTgg             <  7:109472/149‑1 (MQ=255)
                                                                                                                                      cAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTgg             >  5:370691/1‑149 (MQ=255)
                                                                                                                                        aTGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGgcc           <  5:368276/149‑1 (MQ=255)
                                                                                                                                          gCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGgccgc         >  4:118799/1‑149 (MQ=255)
                                                                                                                                          atgaCCTTGTGCCGGCGGAACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGaa                    >  8:192878/4‑138 (MQ=255)
                                                                                                                                          atgaCCTTGTGCCGGCGGAACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGaa                    <  7:192878/135‑1 (MQ=255)
                                                                                                                                            gaCCTTGTGCCGGCGGAACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCAGcca       >  8:328793/2‑149 (MQ=255)
                                                                                                                                             aCCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGcc                            >  8:166251/1‑127 (MQ=255)
                                                                                                                                             aCCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGcc                            <  7:166251/127‑1 (MQ=255)
                                                                                                                                                 tGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGCCACCAGc  >  7:42741/1‑149 (MQ=255)
                                                                                                                                                  gTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGCCACCAg   <  7:276233/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCAATCATCACCGACGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGCCACCAGC  >  NC_002947/1976637‑1976930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑