Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,976,789 C→G 8.3% R151R (CGC→CGG)  PP_1770 → 3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,976,7890CG 8.3% 142.7 / 4.6 48R151R (CGC→CGG) PP_17703‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base C (19/25);  new base G (2/2);  total (21/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

TCATCACCGACGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGCCACCAGCCATCTGC  >  NC_002947/1976641‑1976937
                                                                                                                                                    |                                                                                                                                                     
tcatcaCCGACGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGTGTAGAGGCGTCCAATGCCACCTTGTTccgc                                                                                                                                                       <  8:5735/149‑1 (MQ=255)
   tcaCCGACGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCgg                                                                                                                                                    >  2:253366/1‑149 (MQ=255)
      ccGACGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGcac                                                                                                                                                 <  4:142955/149‑1 (MQ=255)
         aCGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAg                                                                                                                                              <  1:253366/149‑1 (MQ=255)
         aCGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAg                                                                                                                                              <  8:95534/149‑1 (MQ=255)
         aCGGCGGCAGCGCCACGGGCAACGTCGGGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGGTTCGTCCCGGGCCACCCCATCGCCGGTTCCGGGCAGAGCGGGGTAGAGGCGTCCAATGGCACCTTGTTCCGCCGGCACAAg                                                                                                                                              <  4:252441/149‑1 (MQ=255)
            tCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTc                                                                                                                                           <  7:84775/149‑1 (MQ=255)
            tCGGCAGCGCCAAGGGCAACGTCGTGCGGGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTGCCGGCGGAACAAGGtg                                                                                                                                           <  3:271607/149‑2 (MQ=255)
               gCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATc                                                                                                                                        <  4:77391/149‑1 (MQ=255)
                 aGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCt                                                                                                                                      <  5:184925/149‑1 (MQ=255)
                             aaCGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGc                                                                                                                          >  5:124304/1‑149 (MQ=255)
                                   gtgcgtgAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAAc                                                                                                                    <  6:292317/149‑1 (MQ=255)
                                     gcgtgAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCCTCGCCGGTTCCGAGCAGAGCGGGGTAAAGGCGTCCAATGCCACCATGTTCCGCCGGGACAAGGTCATCCACCCCCCGCTGGCCGAAACCg                                                                                                                  >  4:151193/1‑149 (MQ=255)
                                     gcgtgAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCAcg                                                                                                                                  <  6:108871/133‑1 (MQ=255)
                                     gcgtgAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCAcg                                                                                                                                  >  5:108871/1‑133 (MQ=255)
                                                    cGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGca                                                                                                                                                  <  8:196807/102‑1 (MQ=255)
                                                    cGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGca                                                                                                                                                  >  7:196807/1‑102 (MQ=255)
                                                    cGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTgg                                                                                                   <  1:254122/149‑1 (MQ=255)
                                                     gTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGt                                                                                                  >  4:161759/1‑149 (MQ=255)
                                                           ggTGCCCGCCTGGCGCGCTTCGTCCCGGTCCACCGCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCTCCGGCACAAGGTCATCCTCACGCCGCTTGCCGAAACCGACCCCCGCGCGCTGGTCCTgg                                                                                             <  8:162752/148‑1 (MQ=255)
                                                           ggTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGt                                                                                            >  8:276233/1‑149 (MQ=255)
                                                               cccGCCTGCCGCGCTTCGTCCCGGGCCACACCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCATGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGAc                                                                                        >  5:278229/1‑149 (MQ=255)
                                                                ccGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGAcc                                                                                       >  2:286203/1‑149 (MQ=255)
                                                                       ccGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCtg                                                                                  <  3:161759/147‑1 (MQ=255)
                                                                                 tCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTg                                                                     >  6:221441/1‑149 (MQ=255)
                                                                                     gggCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTgc                                                                         >  6:192132/1‑141 (MQ=255)
                                                                                     gggCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTgc                                                                         <  5:192132/141‑1 (MQ=255)
                                                                                         cACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGa                                                             <  6:124304/149‑1 (MQ=255)
                                                                                           ccccATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGAtg                                                           >  6:368276/1‑149 (MQ=255)
                                                                                                         ccGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCTGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGAtgtg                                                         <  2:337377/137‑1 (MQ=255)
                                                                                                         ccGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGAtgtg                                                         >  1:337377/1‑137 (MQ=255)
                                                                                                                gagCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAgc                                      <  1:188218/149‑1 (MQ=255)
                                                                                                                gagCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGGTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAgc                                      <  5:221441/149‑1 (MQ=255)
                                                                                                                gagCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCACGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAgc                                      >  5:185600/1‑149 (MQ=255)
                                                                                                                   cGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTGCTGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAgcgc                                    <  1:256694/149‑1 (MQ=255)
                                                                                                                        tAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCACGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGAc                              <  6:185600/149‑1 (MQ=255)
                                                                                                                        gcgagGCGTCCAATGCCTCCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCTTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGAc                              <  6:232109/147‑1 (MQ=255)
                                                                                                                               gTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCAGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTAc                       >  8:39774/1‑149 (MQ=255)
                                                                                                                                  cAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTgg                    <  7:109472/149‑1 (MQ=255)
                                                                                                                                  cAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTgg                    >  5:370691/1‑149 (MQ=255)
                                                                                                                                    aTGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGgcc                  <  5:368276/149‑1 (MQ=255)
                                                                                                                                      gCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGgccgc                >  4:118799/1‑149 (MQ=255)
                                                                                                                                      atgaCCTTGTGCCGGCGGAACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGaa                           <  7:192878/135‑1 (MQ=255)
                                                                                                                                      atgaCCTTGTGCCGGCGGAACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGaa                           >  8:192878/4‑138 (MQ=255)
                                                                                                                                        gaCCTTGTGCCGGCGGAACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCAGcca              >  8:328793/2‑149 (MQ=255)
                                                                                                                                         aCCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGcc                                   <  7:166251/127‑1 (MQ=255)
                                                                                                                                         aCCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGcc                                   >  8:166251/1‑127 (MQ=255)
                                                                                                                                             tGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGCCACCAGc         >  7:42741/1‑149 (MQ=255)
                                                                                                                                              gTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGCCACCAg          <  7:276233/147‑1 (MQ=255)
                                                                                                                                                    ccgGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTgg                                                        <  7:18773/95‑1 (MQ=255)
                                                                                                                                                    ccgGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTgg                                                        >  8:18773/1‑95 (MQ=255)
                                                                                                                                                    ccgGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGCCACCAGCCATCTGc  <  6:370691/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCATCACCGACGTCGGCAGCGCCAAGGGCAACGTCGTGCGTGAGGCGCGTGCCGTCTTCGGTGCCCGCCTGCCGCGCTTCGTCCCGGGCCACCCCATCGCCGGTTCCGAGCAGAGCGGGGTAGAGGCGTCCAATGCCACCTTGTTCCGCCGGCACAAGGTCATCCTCACGCCGCTGGCCGAAACCGACCCCGCCGCGCTGGTCCTGGTTGACCGCCTG‑TGGCGTGCGCTGGACGCCGATGTGGAGCACATGTCGGTCGAGCGCCATGACGAAGTACTGGCCGCCACCAGCCATCTGC  >  NC_002947/1976641‑1976937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑