Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,991,884	T→C	12.9%	L1045P (CTC→CCC)	PP_1780 →	mannosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,991,884	0	T	C	12.9%	156.2 / 11.8	62	L1045P (CTC→CCC)	PP_1780	mannosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (25/29);  new base C (4/4);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGGGTATCGCTCAGGATGACCTGGTCATTTCGTGTTTCGGCTTCATTCTTGCCCACAAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGCCTGCGCCTGATGAGCGCAGCGGATTACGTGATCTTCCC  >  NC_002947/1991738‑1992031                                                                                                                                                    |                                                                                                                                                    gTTGGGTATCGCTCAGGATGACCTGGTCATTTCGTGTTTCGGCTTCATTCTTGCCCACAAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTc                                                                                                                                                    >  1:131933/1‑148 (MQ=255)  ttGGGTATCGCTCAGGATGACCTGGTCATTTCGTGTTTCGGCTTCATTCTTGCCCACAAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGa                                                                                                                                                  >  6:136455/1‑149 (MQ=255)           gCTCAGGATGACCTGGTCATTTCGTGTTTCGGCTTCATTCTTGCCCACAAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTGCATCCTCGATGCGCGCa                                                                                                                                          <  1:236019/148‑1 (MQ=255)            cTCAGGATGACCTGGTCATTTCGTGTTTCGGCTTCATTCTTGCCCACAAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGc                                                                                                                                        >  6:130814/1‑149 (MQ=255)             tCAGGATGACCTGGTCATTTCGTGTTTCGGCTTCATTCTTGCCCACAAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGc                                                                                                                                        >  4:64979/1‑148 (MQ=255)                ggATGACCTGGTCATTTCGTGTTTCGGCTTCATTCTTGCCCACAAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAAc                                                                                                                                    <  5:136455/149‑1 (MQ=255)                ggATGACCTGGTCATTTCGTGTTTCGGCTTCATTCTTGCCCACAAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCCCGATGCGCGCAGCGAAc                                                                                                                                    <  2:321801/149‑1 (MQ=255)                        tGGTCATTTCGTGTTTCGGCTTCATTCTTGCCCACAAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTg                                                                                                                            >  2:160761/1‑149 (MQ=255)                           tCATTTCGTGTTTCGGCTTCATTCTTGCCCACAAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATTTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGAt                                                                                                                         <  1:160761/149‑1 (MQ=255)                             aTTTCGTCTTTCGGCTTCATTCTTGCCCACAAGGGTATCGACACGCTGATCGAGACCAATCTGACGTTGGCGCAAGAAACAGGCCGCAAAGTTCACATGATCGGTT‑TGCCCTCCAGCCCCGATGCGCGCAGCGAACCGACACTGGAGGc                                                                                                                       >  8:228547/1‑149 (MQ=255)                              tttCGTGTTTCGGCTTCATTCTTGCCCACAAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGAt                                                                                                                                                 <  1:50831/122‑1 (MQ=255)                              tttCGTGTTTCGGCTTCATTCTTGCCCACAAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGAt                                                                                                                                                 >  2:50831/1‑122 (MQ=255)                                     tttCGGCTTCATTCTTGCCCACAAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCCCGATGCGCGCAGCGAACAGACACTGGATGCTTACCggg                                                                                                               >  2:74272/1‑149 (MQ=255)                                       tCGGCTTCATTCTTGCCCACAAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGc                                                                                                              <  7:173139/148‑1 (MQ=255)                                             tCATTCTTGCCCACAAGGGGATCGACACGCTGAGCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCg                                                                                                       <  5:360537/149‑1 (MQ=255)                                                ttCTTGCCCACAAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGtt                                                                                                    <  5:130814/149‑1 (MQ=255)                                                       ccACAAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTg                                                                                             >  2:319806/1‑149 (MQ=255)                                                         acaAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGaa                                                                                                                                     <  5:85085/107‑1 (MQ=255)                                                         acaAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGaa                                                                                                                                     >  6:85085/1‑107 (MQ=255)                                                           aaGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGgtgt                                                                                         <  7:197432/149‑1 (MQ=255)                                                           aaGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGgtgt                                                                                         <  3:64979/149‑1 (MQ=255)                                                              ggTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACTTGCTCGGTT‑TGCACTCCATCCCCGATGCGCGCAGCTAACAGACACTGTATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGa                                                                                      <  7:228547/149‑1 (MQ=255)                                                               gTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCg                                                                                                       <  3:183559/131‑1 (MQ=255)                                                               gTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCg                                                                                                       >  4:183559/1‑131 (MQ=255)                                                                    gACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGt                                                                                                     >  2:152339/1‑128 (MQ=255)                                                                    gACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGt                                                                                                     <  1:152339/128‑1 (MQ=255)                                                                     acacGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGAc                                                                                  >  7:219328/1‑146 (MQ=255)                                                                     acacGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCAGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGAc                                                                                  >  6:292725/1‑146 (MQ=255)                                                                        cGCTTATCGAGACGATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGGACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATc                                                                            <  5:75231/149‑1 (MQ=255)                                                                         gCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTGCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCa                                                                           <  6:358895/149‑1 (MQ=255)                                                                              tCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTgg                                                                      <  1:319806/149‑1 (MQ=255)                                                                              tCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCCCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTgg                                                                      <  1:74272/149‑1 (MQ=255)                                                                                  gaCCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTa                                                                  >  6:266470/1‑149 (MQ=255)                                                                                   aCCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTAc                                                                 >  4:51526/1‑149 (MQ=255)                                                                                            ccGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTTGTGCACTCCATCCTCGATGCGCGCAGCGAACAGCCCCTGGATGATTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACATGGGTTACGGATTTcc                                                         >  1:251804/1‑149 (MQ=255)                                                                                              gTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGcc                                                      >  8:188318/1‑149 (MQ=255)                                                                                                 ggCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGcccc                                                    <  8:219328/148‑1 (MQ=255)                                                                                                    gcAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTg                                                                                             <  6:354261/104‑1 (MQ=255)                                                                                                    gcAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATc                                                 >  5:243692/1‑148 (MQ=255)                                                                                                      aaGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAg                                              >  1:169330/1‑149 (MQ=255)                                                                                                      aaGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAg                                              <  7:188318/149‑1 (MQ=255)                                                                                                       aGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAg                                              >  1:131726/1‑148 (MQ=255)                                                                                                         aaaCAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCCCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAg                                           >  1:25737/1‑149 (MQ=255)                                                                                                           aCAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCACCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTg                                         >  4:59253/1‑149 (MQ=255)                                                                                                            cAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGc                                        >  1:60327/1‑149 (MQ=255)                                                                                                             aGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCCCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGcc                                       >  2:180919/1‑149 (MQ=255)                                                                                                                   cAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGCCTGCGcc                                 <  5:292725/149‑1 (MQ=255)                                                                                                                    aaTGTTCACATGCTCGGTT‑TGCACTCCACCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGCCTGCGCCt                                <  3:59253/149‑1 (MQ=255)                                                                                                                          cacaTGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGCCTGCGCCtgatga                           <  5:266470/148‑1 (MQ=255)                                                                                                                          cacaTGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGCCTGCGCCtgatga                           <  2:251804/148‑1 (MQ=255)                                                                                                                           acaTGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGCCTGCGCCTGATGAgc                         <  3:51526/149‑1 (MQ=255)                                                                                                                                     tt‑tGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGCCTGCGCCTGATGAGCGCAGCGGAtt               <  2:131726/149‑1 (MQ=255)                                                                                                                                         gCACTCCATCCCCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGCCTGCGCCTGATGAGCGCAGCGGATTAc             <  2:25737/148‑1 (MQ=255)                                                                                                                                          cACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGCCTGCGCCTGATGAGCGCAGCGGATTACGt           <  3:293989/149‑1 (MQ=255)                                                                                                                                          cACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGGTGAGCTTGGTGTGGATGACAAGATCACCTTGGTTACCGATTTCCGCCCCATCAAGGAGTGCCTGCGCCTGATGAGCGCAGCGGATTACGt           >  6:256847/1‑149 (MQ=255)                                                                                                                                            cTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGCCTGCGCCtgatga                           <  3:354066/131‑1 (MQ=255)                                                                                                                                            cTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGCCTGCGCCtgatga                           >  4:354066/1‑131 (MQ=255)                                                                                                                                                aTCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGCCTGCGCCtg                               <  2:179452/123‑1 (MQ=255)                                                                                                                                                aTCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGCCTGCGCCtg                               >  1:179452/1‑123 (MQ=255)                                                                                                                                                aTCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGCCTGCGCCTGATGAGCGCAGCGGATTACGTGATCtt     <  2:60327/149‑1 (MQ=255)                                                                                                                                                  ccTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGTCTGCGCCTGATGAGCGCAGCGGATTACGTGATCTTcc   >  5:61993/1‑149 (MQ=255)                                                                                                                                                   cTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGCCTGCGCCTGATGAGCGCAGCGGATTACGTGATCTTccc  <  2:169330/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GTTGGGTATCGCTCAGGATGACCTGGTCATTTCGTGTTTCGGCTTCATTCTTGCCCACAAGGGTATCGACACGCTGATCGAGACCATTCTGCCGTTGGCGCAAGAAACAGGCCGCAATGTTCACATGCTCGGTT‑TGCACTCCATCCTCGATGCGCGCAGCGAACAGACACTGGATGCTTACCGGGCCAGGGCGGTTGAGCTTGGTGTGGATGACAAGATCACCTGGGTTACCGATTTCCGCCCCATCAAGGAGTGCCTGCGCCTGATGAGCGCAGCGGATTACGTGATCTTCCC  >  NC_002947/1991738‑1992031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑