| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 195,539 | G→A | 13.3% | V349I (GTC→ATC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 195,539 | 0 | G | A | 13.3% | 89.9 / 8.7 | 45 | V349I (GTC→ATC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base G (17/22); new base A (2/4); total (19/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTA > NC_002947/195397‑195687 | ccTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCAc > 3:318482/1‑147 (MQ=34)ccTGAGCAACCCTGCCCAGACGCAGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAAACCAGGGCAGCGTCGATTTCCAGACACCGGCCAATGACGTCTACAACAACCGTTCGACCGTCATCGTCACCa > 1:180663/1‑149 (MQ=11) tGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCATCACCATc > 7:216053/1‑149 (MQ=21) aGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATc < 8:258531/147‑1 (MQ=34) ccTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGc < 2:98428/149‑1 (MQ=32) gtgaccgtgaccCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGAGAAtt > 5:117657/1‑148 (MQ=17) ccgtgaccCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAg < 7:97899/149‑1 (MQ=35) ccgtgaccCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACAGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAg < 6:122772/149‑1 (MQ=25) cccTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAAACCATGGCAGCGTCGATTTCCAGACACAGGCCAATGACGACTACAACAACGGTTAGACCGTCAGCGTCACAATCTAGAACGCAAACGGCGGGTATTTCGAGCAAcg > 8:262953/1‑147 (MQ=11) tCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGAcccc < 7:31965/149‑1 (MQ=39) gaGGTCTTCACTTTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACGCCGGCCAATGACTTCTACAAGAACGGTTCGACCGTCAGCATGACCATCGAGAACTCCACCGGCGGTAATTTCGAGCAACTGACCGCGAATCCGAc < 5:10322/148‑1 (MQ=14) caCTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCg < 3:152626/119‑1 (MQ=35) caCTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCg > 4:152626/1‑119 (MQ=35) tGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGa < 3:42783/149‑1 (MQ=38) ttGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGa < 6:71584/147‑1 (MQ=37) ggCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCTGCCAATGACGTCTACAACAACTGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCaa < 7:262953/149‑1 (MQ=255) aCCCAGGGCAGCGTCGAGTTCCAGACCCCGGACAATGACGTCTACAACAAAGGTTCGACCGTCAGCGTCACCATCGAGAACGCCCCCGGCGGTAATTTCGAGAACCTGCCCCCGAATACGACCCCGGCTCAGACCACGATCAACCACTc > 1:248537/1‑149 (MQ=11) ccAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCgg < 4:318482/149‑1 (MQ=34) ccAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCgg < 7:130/149‑1 (MQ=34) aGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAGCAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTc < 2:35156/149‑1 (MQ=21) aGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTc < 4:21260/149‑1 (MQ=34) ggCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGCCCCCGGCTCCGACCACGATCAACGACTCGGTCGa > 6:24726/1‑149 (MQ=21) gccgcggcgATTTCCAGACCCCGGCCTATGACGTCTACAACAACGCTGCGACCTTCAGCATCCCCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGac < 8:327196/146‑1 (MQ=11) gCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGac < 4:150676/149‑1 (MQ=35) attCCAGCCCCCGGCCAATGACGTCTACAACAACGCATCGACCGTCAGCGTCACCATCCAGAACGCCACCGGCGTTAATTTCGAGCACCTGACCCCCAATCCCCCCCCGGCTCAGACCACGATCAACGACTCTGTCGACACCACCACcg < 2:180663/148‑1 (MQ=14) cGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACg > 8:108173/1‑149 (MQ=39) aTGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGTCGGAAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACGACCACCGCGACCCTGACGGCGAGc < 6:117657/149‑1 (MQ=255) caacaaCGGTTCGACCGTCAGCATCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGt < 8:216053/147‑1 (MQ=37) aacaacGGTTCAACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCAc < 5:185992/149‑1 (MQ=12) aacaacGGTGCGACCGGCAGCGGCACCATCGAGAACGCCACAGTGGGTAATTTCGAGCAAATGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCAc < 2:3809/149‑1 (MQ=11) caacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGAcc < 5:104542/124‑1 (MQ=38) caacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGAcc > 6:104542/1‑124 (MQ=38) aacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCg > 2:96222/1‑148 (MQ=17) aacGGTTCGACCGTCAGCATCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCg > 7:173724/1‑148 (MQ=37) acGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGaa < 1:226939/149‑1 (MQ=17) tCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcgg > 5:325954/1‑149 (MQ=17) aCCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt > 2:351891/1‑149 (MQ=37) aCCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt > 8:50763/1‑149 (MQ=37) ccGTCAGCGTCACCATCGAGAACGCCACAGGCGTTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGAGTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTg < 3:110012/149‑1 (MQ=255) ccGTCAGCATCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTg < 8:173724/149‑1 (MQ=38) cGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGa > 6:44850/1‑149 (MQ=17) gTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGa > 4:212254/1‑148 (MQ=17) tCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTCAt > 6:112787/1‑148 (MQ=12) cGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACtt < 3:139177/149‑1 (MQ=17) cGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACt > 4:139177/1‑148 (MQ=17) gTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGCCTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTa > 8:329241/1‑149 (MQ=255) gTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAATGCGGCGTGATCACTTa < 7:339586/149‑1 (MQ=12) | CCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTA > NC_002947/195397‑195687 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |