Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,022,441 C→T 9.7% Y180Y (TAC→TAT)  gmd → GDP‑mannose 4,6‑dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,022,4410CT 9.7% 108.9 / 3.2 41Y180Y (TAC→TAT) gmdGDP‑mannose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base C (16/21);  new base T (2/2);  total (18/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

CCGCTTCTATCAGGCGTCCACCTCCGAGATGTTCGGCAAGGTGCAAGAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGACCCGCAAAATCACCGACTCCGTCGCCAAAATCAAGCTGGGCCTGCTCGACAAGCTCGAGCTGGGTAACCTTGATGCCAAGC  >  NC_002947/2022294‑2022589
                                                                                                                                                   |                                                                                                                                                    
ccGCTTCTATCAGGCGTCCACCTCCGAGATGTTCGGCAAGGTGCAAGAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTAc                                                                                                                                                      >  7:157394/1‑148 (MQ=255)
  gCTTCTATCAGGCGTCCACCTCCGAGATGTTCGGCAAGGTGCAAGAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAAc                                                                                                                                                   <  2:23127/149‑1 (MQ=255)
  gCTTCTATCAGGCGTCCACCTCCGAGATGTTCGGCAAGGTGCAAGAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAAc                                                                                                                                                   >  3:38346/1‑149 (MQ=255)
          cAGGCGTCCACCTCCGAGATGTTCGGCAAGGTGCAAGAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTATAACCTCTTTGc                                                                                                                                           >  3:61885/1‑149 (MQ=255)
               gTCCACCTCCGAGATGTTCGGCAAGGTGCAAGAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGAc                                                                                                                                        >  8:135001/1‑147 (MQ=255)
                  cACCTCCGAGATGTTCGGCAAGGTGCAAGAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTATAACCTCTTTGCGACCAGCg                                                                                                                                   <  4:61885/149‑1 (MQ=255)
                      tCCGAGATGTTCGGCAAGGTGCAAGAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTAt                                                                                                                               >  5:149685/1‑149 (MQ=255)
                        cGAGATGTTCGGCAAGGTGCAAGAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATc                                                                                                                              <  7:135001/148‑1 (MQ=255)
                          agaTGTTCGGCAAGGTGCAAGAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTGTCTTg                                                                                                                           <  6:117926/149‑1 (MQ=255)
                          agaTGTTCGGCAAGGTGCAAGAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTg                                                                                                                           <  8:157394/149‑1 (MQ=255)
                            aTGTTCGGCAAGGTGCAAGAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGtt                                                                                                                         <  3:152051/149‑1 (MQ=255)
                                   gCAAGGTGCAAGAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCAc                                                                                                                  <  7:15139/149‑1 (MQ=255)
                                           cAAGAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGc                                                                                                           >  8:281254/1‑148 (MQ=255)
                                            aaGAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCAc                                                                                                                  >  8:317359/1‑140 (MQ=255)
                                            aaGAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCAc                                                                                                                  <  7:317359/140‑1 (MQ=255)
                                              gAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCAc                                                                                                                  >  4:188999/1‑138 (MQ=255)
                                              gAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCAc                                                                                                                  <  3:188999/138‑1 (MQ=255)
                                                  ttCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTgcgc                                                                                                   <  6:149685/149‑1 (MQ=255)
                                                          gTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGa                                                                                           <  7:281254/149‑1 (MQ=255)
                                                           tCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAg                                                                                          >  5:28674/1‑149 (MQ=255)
                                                             gAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGtt                                                                                        <  4:38346/149‑1 (MQ=255)
                                                               aaCTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCg                                                                                      <  6:371092/149‑1 (MQ=255)
                                                                aCTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGt                                                                                     <  6:28674/149‑1 (MQ=255)
                                                                    ccccGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGAc                                                                                  <  3:139695/148‑1 (MQ=255)
                                                                     cccGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGAccc                                                                                >  4:333738/1‑149 (MQ=255)
                                                                                 gcgcAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGAc                                                                                  >  8:238/1‑135 (MQ=255)
                                                                                 gcgcAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGAc                                                                                  <  7:238/135‑1 (MQ=255)
                                                                                                   ggCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGACCCGCAAAATCACCGACTCCGTCGCCAAAATc                                                   >  2:233129/1‑148 (MQ=255)
                                                                                                    gCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGACCCGCAAAATCACCGACTCCGTCGCCAAAATc                                                   >  7:237535/1‑147 (MQ=255)
                                                                                                     ccAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGACCCGCAAAATCACCGACTCCGTCGCCAAAATCAAg                                                <  3:249213/149‑1 (MQ=255)
                                                                                                                gCCCACTGGATGACCATCAACTACCGCGAGTCGTATAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGACCCGCAAAATCACCGACTCCGTCGCCAAAATCAAGCTGGGCCTGCt                                     >  1:88495/1‑149 (MQ=255)
                                                                                                                gCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCACCCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGACCCGCAAAATCACCGACTCCGTCGCCAAAATCAAGCTGGGCCTGCt                                     >  7:327501/1‑149 (MQ=255)
                                                                                                                gCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGACCCGCAAAATCACCGACTCCGTCGCCAAAATCAAGCTGGGCCTGCt                                     >  7:39170/1‑149 (MQ=255)
                                                                                                                   cACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGACCCGCAAAATCACCGACTCCGTCGCCAAAATCAAGCTg                                             <  4:181217/138‑1 (MQ=255)
                                                                                                                    aCTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGACCCGCAAAATCACCGACTCCGTCGCCAAAATCAAGCTGGGCCTGCTCGa                                  >  5:300375/1‑148 (MQ=255)
                                                                                                                        gATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGACCCGCAAAATCACCGACTCCGTCGCCAAAATCAAGCTGGGCCTGCTCGACaa                               <  3:333738/147‑1 (MQ=255)
                                                                                                                        gATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGACCCGCAAAATCACCGACTCCGTCGCCAAAATCAAGCTGGGCCTGCTCGACAAGc                             >  8:309164/1‑149 (MQ=255)
                                                                                                                            aCCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTTGCCACTGCGCGGCCGTGAGTTCGTGACCCGCAAAATCACCGACTCCGTCGCCAAAATCAAGCTGGGCCTGCTCGACAAGCTCGa                         <  4:111931/149‑1 (MQ=255)
                                                                                                                               aTCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGACCCGCAAAATCACCGACTCCGTCGCCAAAATCAAGCTGGGCCTGCTCGACAAGCTCGAGCt                      <  8:39170/149‑1 (MQ=255)
                                                                                                                                  aaCTACCGCGAGTCGTATAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGACCCGCAAAATCACCGACTCCGTCGCCAAAATCAAGCTGGGCCTGCTCGACAAGCTCGAGCTgg                    <  2:88495/148‑1 (MQ=255)
                                                                                                                                  aaCTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGACCCGCAAAATCACCGACTCCGTCGCCAAAATCAAGCTGGGCCTGCTCGACAAGCTc                           >  3:357014/1‑141 (MQ=255)
                                                                                                                                  aaCTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGACCCGCAAAATCACCGACTCCGTCGCCAAAATCAAGCTGGGCCTGCTCGACAAGCTc                           <  4:357014/141‑1 (MQ=255)
                                                                                                                                                   cAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGACCCGCAAAATCACCGACTCCGTCGCCAAAATCAAGCTGGGCCTGCTCGACAAGCTCGAGCTGGGTAACCTTGATGCCAAGc  >  3:207044/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCGCTTCTATCAGGCGTCCACCTCCGAGATGTTCGGCAAGGTGCAAGAAATTCCACAGGTCGAAACTACCCCGTTCTACCCGCGCAGTCCCTATGGTGTGGCCAAGCTCTACGCCCACTGGATGACCATCAACTACCGCGAGTCGTACAACCTCTTTGCGACCAGCGGTATCTTGTTCAACCACGAGTCGCCACTGCGCGGCCGTGAGTTCGTGACCCGCAAAATCACCGACTCCGTCGCCAAAATCAAGCTGGGCCTGCTCGACAAGCTCGAGCTGGGTAACCTTGATGCCAAGC  >  NC_002947/2022294‑2022589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑