| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,063,220 | G→T | 13.2% | intergenic (+77/‑130) | PP_1838 → / → PP_1839 | membrane protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,063,220 | 0 | G | T | 13.2% | 98.0 / 4.4 | 36 | intergenic (+77/‑130) | PP_1838/PP_1839 | membrane protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (13/18); new base T (2/3); total (15/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTGG > NC_002947/2063074‑2063361 | gTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTc < 3:31944/149‑1 (MQ=255) tCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCtgtg < 7:91199/149‑1 (MQ=255) tCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCtgtg < 5:112049/149‑1 (MQ=255) tCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCtgt < 5:92568/148‑1 (MQ=255) aCCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGAGc < 8:55391/149‑1 (MQ=255) gcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGAGCggg < 2:248532/149‑1 (MQ=255) aCCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGCCATTTCACAGGGTTC‑GGACCTTGTGCAGTTCCTGTGCGAGCGGGCaa < 5:130367/149‑1 (MQ=255) agCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCcgc < 3:311305/149‑1 (MQ=255) tgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATggg > 5:329609/1‑149 (MQ=255) gATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCAATCGCGGGCTTGCCCGCTCCCAGAGGCATTGCACAGGGTTC‑GGTCCTTGTGCAGGTGCTGTGCGAGCGGGCAAGCCCGCGAATggggc > 1:283802/1‑147 (MQ=255) aTAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTTCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCCCGAATCGGccc > 6:113363/1‑149 (MQ=255) aaCACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCg > 1:82222/1‑149 (MQ=255) ccgccgGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCCCAGGGGTC‑GGACCTTGTGCAGGTCCTGTGCGAGCGGGCCAGCCCGCGCATGGGCCCCGcac > 7:327321/1‑148 (MQ=255) ccGGAACACCTAAGTAACCGGGGGCCGCTTGGGGGCCCTTGCGGGGGCTTGCCCGCTCCCACTGGCTTTCCACTGGGTTC‑GCACCTTGTGCTGTCCCTGTGCTCGCGGGAATGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGa < 4:133039/148‑1 (MQ=255) ccGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGGGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATc < 1:50686/149‑1 (MQ=255) aGTACACCGGGGCCGCGTTGCGGCCCATGCGCGGGCGTGCCCGCGCCCCCAGGCATTGCACCGGGTTC‑GGCCCTTGTGCAGGGCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCg < 8:327321/149‑1 (MQ=38) gTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGCGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGc > 5:330297/1‑149 (MQ=255) aaCCGGGGCCGCGTTGCGGCCCATTCGCGGGCATTCCCCCGCCCCCAGGCCATGGACAGGGGTA‑GGCCCATTTTGAGGTACAGTTCGCGCCGGCCAGCCCGCCCATTGGCCCCCCACAAATTTAACCGACGGAAAATTTTAACCGccc > 4:101156/1‑148 (MQ=255) ccGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTTTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAg < 5:113363/149‑1 (MQ=255) gcgTTGCGGCCCATTCGCGGGCTTGCCCGCGCCCACAGGCATTGCACAGGGTTCGGGGCCTTGTGCAGGGCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTGCCGGACTGAACATTATGCCCGCCCCAAgggccgg > 1:98742/1‑144 (MQ=37) ttGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTAtt < 8:72986/149‑1 (MQ=255) ttGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTAtt < 7:274036/149‑1 (MQ=255) ttGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTAtt < 2:82222/149‑1 (MQ=255) ccATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCGCCACAATTTTACCGGACTGAACATTATAACCCCCCCAAGGTCAGTTATTCAGGCa > 6:292081/1‑148 (MQ=37) aTTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATca > 7:19313/1‑149 (MQ=255) gCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTg < 8:19313/149‑1 (MQ=255) ccGCTCGCACAGGACCTGCACAAGGTCC‑GAACCCTGTGCAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTc > 2:338255/1‑149 (MQ=18) ccGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTc > 5:160940/1‑149 (MQ=255) cccccACCGGCATTGCACCGGTCTC‑GCCCTTCGTGCCGGTCCTGGGCGCGCGGGCATGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGtt < 2:283802/147‑1 (MQ=255) ccACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGAc > 3:334420/1‑149 (MQ=255) cacaGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCACCGCACAATTTTCACGGAATGAACATTATCCCCGACCCAAGGTCAGTTATTAAGGCATCACACACCTGGTGTTAGTTGaat > 5:613/1‑147 (MQ=255) gacctGCACAAGGTCC‑GAACCCTGTGCAATGCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCAGGACTGAACATTATCCCCGCCCCAAGTTCAGTTATTCAGGCATCACACACCTGGTTTTCGTTTACAgatcg > 4:10275/5‑146 (MQ=21) gaactGCACAAGGTCC‑GAACCCTGTGCAATGCCTGTGCGAGCTGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGa < 3:10275/138‑1 (MQ=25) gTTC‑GGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAAt < 6:329609/149‑1 (MQ=255) gtgCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTgg < 6:330297/148‑1 (MQ=255) gtgCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTg < 3:101156/147‑1 (MQ=255) | GTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTC‑GGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGTGG > NC_002947/2063074‑2063361 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |