| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 195,559 | C→A | 37.3% | T355T (ACC→ACA) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 195,559 | 0 | C | A | 37.3% | 67.2 / 59.9 | 59 | T355T (ACC→ACA) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (16/21); new base A (10/12); total (26/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01 | |||||||||||
GTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGC > NC_002947/195422‑195703 | gtgaccgtgaccCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGAGAAtt > 5:117657/1‑148 (MQ=17) ccgtgaccCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAg < 7:97899/149‑1 (MQ=35) ccgtgaccCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACAGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAg < 6:122772/149‑1 (MQ=25) cccTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAAACCATGGCAGCGTCGATTTCCAGACACAGGCCAATGACGACTACAACAACGGTTAGACCGTCAGCGTCACAATCTAGAACGCAAACGGCGGGTATTTCGAGCAAcg > 8:262953/1‑147 (MQ=11) tCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGAccc‑c < 7:31965/149‑1 (MQ=39) gaGGTCTTCACTTTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACGCCGGCCAATGACTTCTACAAGAACGGTTCGACCGTCAGCATGACCATCGAGAACTCCACCGGCGGTAATTTCGAGCAACTGACCG‑CGAATCCGAc < 5:10322/148‑1 (MQ=14) caCTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCg > 4:152626/1‑119 (MQ=35) caCTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCg < 3:152626/119‑1 (MQ=35) tGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGa < 3:42783/149‑1 (MQ=38) ttGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGa < 6:71584/147‑1 (MQ=37) ggCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCTGCCAATGACGTCTACAACAACTGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCaa < 7:262953/149‑1 (MQ=255) aCCCAGGGCAGCGTCGAGTTCCAGACCCCGGACAATGACGTCTACAACAAAGGTTCGACCGTCAGCGTCACCATCGAGAACGCCCCCGGCGGTAATTTCGAGAACCTGCCCC‑CGAATACGACCCCGGCTCAGACCACGATCAACCACTc > 1:248537/1‑149 (MQ=11) ccAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCgg < 7:130/149‑1 (MQ=34) ccAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCgg < 4:318482/149‑1 (MQ=34) aGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAGCAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTc < 2:35156/149‑1 (MQ=21) aGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTc < 4:21260/149‑1 (MQ=34) ggCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGCCCCCGGCTCCGACCACGATCAACGACTCGGTCGa > 6:24726/1‑149 (MQ=21) gccgcggcgATTTCCAGACCCCGGCCTATGACGTCTACAACAACGCTGCGACCTTCAGCATCCCCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGac < 8:327196/146‑1 (MQ=11) gCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGac < 4:150676/149‑1 (MQ=35) attCCAGCCCCCGGCCAATGACGTCTACAACAACGCATCGACCGTCAGCGTCACCATCCAGAACGCCACCGGCGTTAATTTCGAGCACCTGACCC‑CCAATCCCCCCCCGGCTCAGACCACGATCAACGACTCTGTCGACACCACCACcg < 2:180663/148‑1 (MQ=14) cGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACg > 8:108173/1‑149 (MQ=39) aTGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGTCGGAAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACGACCACCGCGACCCTGACGGCGAGc < 6:117657/149‑1 (MQ=255) caacaaCGGTTCGACCGTCAGCATCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGt < 8:216053/147‑1 (MQ=37) aacaacGGTTCAACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCAc < 5:185992/149‑1 (MQ=12) aacaacGGTGCGACCGGCAGCGGCACCATCGAGAACGCCACAGTGGGTAATTTCGAGCAAATGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCAc < 2:3809/149‑1 (MQ=11) caacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGAcc > 6:104542/1‑124 (MQ=38) caacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGAcc < 5:104542/124‑1 (MQ=38) aacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCg > 2:96222/1‑148 (MQ=17) aacGGTTCGACCGTCAGCATCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCg > 7:173724/1‑148 (MQ=37) acGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGaa < 1:226939/149‑1 (MQ=17) tCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcgg > 5:325954/1‑149 (MQ=17) aCCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt > 8:50763/1‑149 (MQ=37) aCCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt > 2:351891/1‑149 (MQ=37) ccGTCAGCGTCACCATCGAGAACGCCACAGGCGTTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGAGTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTg < 3:110012/149‑1 (MQ=255) ccGTCAGCATCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTg < 8:173724/149‑1 (MQ=38) cGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGa > 6:44850/1‑149 (MQ=17) gTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGa > 4:212254/1‑148 (MQ=17) tCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTCAt > 6:112787/1‑148 (MQ=12) cGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACtt < 3:139177/149‑1 (MQ=17) cGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACt > 4:139177/1‑148 (MQ=17) gTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGCCTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTa > 8:329241/1‑149 (MQ=255) gTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAATGCGGCGTGATCACTTa < 7:339586/149‑1 (MQ=12) cACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGAAGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTaca > 5:311927/1‑149 (MQ=255) cACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCGCGAATCCGCCCCCGGCTCAGCCCACGATCAACGCCTCGGTCGACACCCCTACCCCGACCCTGCCGGCGAGCCCGACGGTCACCGAAGGCGGCGTGATCAc > 1:256112/1‑145 (MQ=11) cACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc < 2:256112/144‑1 (MQ=17) aCCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTaca < 8:22852/148‑1 (MQ=17) aCCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTaca < 6:325954/148‑1 (MQ=17) cATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTCACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCg > 1:59377/1‑149 (MQ=255) aTCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGt < 5:24726/149‑1 (MQ=39) gaACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAccc > 2:359344/1‑149 (MQ=39) aCGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGc < 5:274472/104‑1 (MQ=40) aCGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGc > 6:274472/1‑104 (MQ=40) cGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCt > 7:116027/1‑147 (MQ=39) cGCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGc < 8:132751/103‑1 (MQ=12) cGCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGc > 7:132751/1‑103 (MQ=12) cGCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGa < 6:202562/149‑1 (MQ=17) gCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACAcc > 6:53819/1‑138 (MQ=17) gCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACAcc < 5:53819/138‑1 (MQ=17) gCCACAGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAg > 6:320763/1‑149 (MQ=17) cacaGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGc > 2:93233/1‑148 (MQ=17) cacaGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGc > 8:42301/1‑148 (MQ=17) ccGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCg > 6:95085/1‑104 (MQ=38) ccGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCg < 5:95085/104‑1 (MQ=38) | GTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCC‑CGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGC > NC_002947/195422‑195703 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |