Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,092,380	G→A	7.3%	intergenic (+26/+65)	deaD → / ← ygiD	ATP‑dependent DEAD‑box RNA helicase DeaD/4,5‑DOPA‑dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,092,380	0	G	A	7.3%	197.6 / 4.2	68	intergenic (+26/+65)	deaD/ygiD	ATP‑dependent DEAD‑box RNA helicase DeaD/4,5‑DOPA‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base G (32/31);  new base A (2/3);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

GTGCGCGACAGCTTCAGCTTGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAG  >  NC_002947/2092232‑2092528                                                                                                                                                      |                                                                                                                                                     gTGCGCGACAGCTTCAGCTTGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCgg                                                                                                                                                      >  6:58390/1‑149 (MQ=255)       gACAGCTTCAGCTTGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAgg                                                                                                                                                >  6:164497/1‑149 (MQ=255)               cagcttGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGACCTAAGCCGGGGa                                                                                                                                         <  6:56066/148‑1 (MQ=255)                 gcttGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGAACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGAtt                                                                                                                                       >  1:280834/1‑149 (MQ=255)                   ttGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGAttttt                                                                                                                                    >  8:143887/1‑149 (MQ=255)                        cGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTAc                                                                                                                               <  3:95977/149‑1 (MQ=255)                         gAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTAc                                                                                                                               <  7:143887/148‑1 (MQ=255)                           gCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTg                                                                                                                            >  8:268506/1‑149 (MQ=255)                              gCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTAc                                                                                                                         >  4:83139/1‑149 (MQ=255)                                cGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCg                                                                                                                       >  7:276532/1‑149 (MQ=255)                                cGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCg                                                                                                                       >  7:280251/1‑149 (MQ=255)                                 ggAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGATGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCgg                                                                                                                      <  8:280251/149‑1 (MQ=255)                                 ggAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGAttttt                                                                                                                                    >  4:104059/1‑135 (MQ=255)                                 ggAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCgg                                                                                                                      >  8:316498/1‑149 (MQ=255)                                 ggAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCgg                                                                                                                      >  3:233213/1‑149 (MQ=255)                                 ggAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGACCTAAGCCGGGGAttt                                                                                                                                      <  2:248805/133‑1 (MQ=255)                                    aGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGccc                                                                                                                   <  5:164497/149‑1 (MQ=255)                                        ggCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTc                                                                                                               >  7:104045/1‑149 (MQ=255)                                        ggCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTc                                                                                                               >  3:220797/1‑149 (MQ=255)                                          ccTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTcgc                                                                                                             <  4:220797/149‑1 (MQ=255)                                          ccTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTcgc                                                                                                             <  8:104045/149‑1 (MQ=255)                                          ccTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGACCTAAGCCGGGGAttt                                                                                                                                      <  6:223803/124‑1 (MQ=255)                                          ccTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGACCTAAGCCGGGGAttt                                                                                                                                      >  5:223803/1‑124 (MQ=255)                                              gagaAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGc                                                                                                         <  7:316498/149‑1 (MQ=255)                                               agaAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGAtttttt                                                                                                                                   <  6:228681/122‑1 (MQ=255)                                               agaAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGAtttttt                                                                                                                                   >  5:228681/1‑122 (MQ=255)                                                    ctgctgTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTcgc                                                                                                             <  6:59823/139‑1 (MQ=255)                                                    ctgctgTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTcgc                                                                                                             >  5:59823/1‑139 (MQ=255)                                                           ccAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAaga                                                                                            <  5:220711/149‑1 (MQ=255)                                                            cAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCgg                                                                                                           <  1:243440/133‑1 (MQ=255)                                                            cAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCgg                                                                                                           >  2:243440/1‑133 (MQ=255)                                                             aaGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAgg                                                                                          >  3:298895/1‑149 (MQ=255)                                                               gCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAagag                                                                                           <  2:25219/146‑1 (MQ=255)                                                               gCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAagag                                                                                           >  1:25219/1‑146 (MQ=255)                                                                cTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCTCTTCGCGGGCAAGCCCGCGAAAGGGccg                                                                                       <  3:83139/149‑2 (MQ=39)                                                                   aaGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTcgcg                                                                                                            <  5:249631/125‑1 (MQ=255)                                                                   aaGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTcgcg                                                                                                            >  6:249631/1‑125 (MQ=255)                                                                   aaGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACAGGCCCTTTCGCGGGCTTGCACGCGAAGAGGCCCtt                                                                                     >  2:29631/1‑148 (MQ=255)                                                                    aGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCa                                                                                   <  5:58390/149‑1 (MQ=255)                                                                               cgTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGa                                                                         <  7:259854/149‑1 (MQ=255)                                                                               cgTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGa                                                                         <  2:280834/149‑1 (MQ=255)                                                                                gTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGAt                                                                        <  7:268506/149‑1 (MQ=255)                                                                                    ctggcAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGAt                                                                    <  8:102873/149‑1 (MQ=255)                                                                                     tggcAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATg                                                                   >  5:107102/1‑149 (MQ=255)                                                                                         aaGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCa                                                               >  1:29977/1‑149 (MQ=255)                                                                                           gcagcTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATc                                                             >  1:161491/1‑149 (MQ=255)                                                                                           gcagcTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGACGCCCTTCAGTCGAACCGATAGATGTCCATc                                                             <  1:29631/149‑1 (MQ=255)                                                                                             agcTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATccc                                                           <  4:233213/149‑1 (MQ=255)                                                                                              gcTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGAt                                                                    >  1:149921/1‑139 (MQ=255)                                                                                              gcTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGAt                                                                    <  2:149921/139‑1 (MQ=255)                                                                                               cTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCa                                                         >  8:105043/1‑149 (MQ=255)                                                                                                       gCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCcgc                                                                                                 >  1:335425/1‑101 (MQ=255)                                                                                                       gCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCcgc                                                                                                 <  2:335425/101‑1 (MQ=255)                                                                                                          tCGCTACCGCGAGGATTGATCCTGGCGTAACGGAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCa                                                                                   >  6:74439/1‑112 (MQ=255)                                                                                                          tCGCTACCGCGAGGATTGATCCTGGCGTAACGCAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCa                                                                                   <  5:74439/112‑1 (MQ=255)                                                                                                            gCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAggg                                             <  8:276532/148‑1 (MQ=255)                                                                                                            gCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGt                                            <  2:29977/149‑1 (MQ=255)                                                                                                                ccGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAAc                                        <  4:298895/149‑1 (MQ=255)                                                                                                                        aTTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATg                                >  7:70163/1‑149 (MQ=255)                                                                                                                            aTCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATccc                                                           >  7:202133/1‑118 (MQ=255)                                                                                                                            aTCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATccc                                                           <  8:202133/118‑1 (MQ=255)                                                                                                                                  gCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGc                        <  2:161491/147‑1 (MQ=255)                                                                                                                                         gAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAAc                   >  8:327505/1‑145 (MQ=255)                                                                                                                                         gAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAAc                   <  7:327505/145‑1 (MQ=255)                                                                                                                                         gAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGc               >  2:281947/1‑149 (MQ=255)                                                                                                                                           aaaaaTCCCCGACCTAAGCCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTc             >  7:34726/1‑149 (MQ=255)                                                                                                                                            aaaaTCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTcc            <  7:105043/149‑1 (MQ=255)                                                                                                                                            aaaaTCCCCGGCTTAGGTCGGGGA‑TTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTccc           >  6:74814/1‑149 (MQ=255)                                                                                                                                                 ccccGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGcc       >  7:192536/1‑149 (MQ=255)                                                                                                                                                      gCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCTCCACGCCGAACTTGCTCCCGGCCCCAAg  >  3:189873/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                     GTGCGCGACAGCTTCAGCTTGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGG‑ACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAG  >  NC_002947/2092232‑2092528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑