Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,099,701	G→A	5.5%	N63N (AAC→AAT)	PP_1875 ←	two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,099,701	0	G	A	5.5%	223.2 / 2.3	73	N63N (AAC→AAT)	PP_1875	two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base G (32/37);  new base A (2/2);  total (34/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

GTACCGTTTCGTTGTTGTCGATCAGCCGTGCGCGGATGACGGCCGGCGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCGACCAGGACTGTCAACCGGGCTTGCTTGTATGACAGGCGATTGGTGAACTTGATATCCATGA  >  NC_002947/2099559‑2099849                                                                                                                                                |                                                                                                                                                     gTACCGTTTCGTTGTTGTCGATCAGCCGTGCGCGGATGACGGCCGGCGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTACGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGttcact                                                                                                                                                >  1:103553/1‑149 (MQ=255)  tACCGTTTCGTTGTTGTCGATCAGCCGTGCGCGGATGACGGCCGGCGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGttcactt                                                                                                                                               >  1:168224/1‑149 (MQ=255)      gTTTCGTTGTTGTCGATCAGCCGTGCGCGGATGACGGCCGGCGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACg                                                                                                                                           >  3:215379/1‑149 (MQ=255)          cGTTGTTGTCGATCAGGCGTGCGCGGATGACGGCCGGCGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGtt                                                                                                                                        <  3:123/148‑1 (MQ=255)          cGTTGTTGTCGATCAGCCGTGCGCGGATGACGGCCGGCGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGtt                                                                                                                                        <  4:259955/148‑1 (MQ=255)          cGTTGTTGGCGATCAGGCGTTGGCGGATGACGGCCGGCGACTGCAGCAGGCCGGGGGTCAGCTCCAGCGGCATTTCCGACTCGATGTTGTACGCGATGCGCGAGGCCGGGTTGATGGCTGATTTGCAGCAACGCGTTCACTTCACGGt                                                                                                                                         <  1:232048/148‑1 (MQ=255)           gttgttGTCGATCAGCCGTGCGCGGATGACGGCGGGCGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGa                                                                                                                                      <  8:37466/149‑1 (MQ=255)            ttgttgTCGATCAGCCGTGCGCGGATGACGGCCGGCGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGAt                                                                                                                                     >  8:327355/1‑149 (MQ=255)               ttgtCGATCAGCCGTGCGCGGATGACGGCCGGCGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATgg                                                                                                                                   <  7:196409/148‑1 (MQ=255)                 gtCGATCAGCCGTGCGCGGATGACGGCCGGCGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGAcg                                                                                                                                >  8:145919/1‑149 (MQ=255)                  tCGATCAGCCGTGCGCGGATGACGGCCGGCGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGAcgc                                                                                                                               <  3:267516/149‑1 (MQ=255)                            gTGCGCGGTTGACGGCCGGCGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTg                                                                                                                     >  5:235871/1‑149 (MQ=255)                             tGCGCGGATGACGGCCGGCGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTgg                                                                                                                    <  2:103553/149‑1 (MQ=255)                             tGCGCGGATGACGGCCGGCGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTgg                                                                                                                    <  2:169347/149‑1 (MQ=255)                                  ggATGACGGCCGGCGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATaa                                                                                                               >  8:306480/1‑149 (MQ=255)                                      gACGGCCGGCGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGa                                                                                                           >  8:63071/1‑149 (MQ=255)                                       aCGGCCGGCGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGa                                                                                                           <  1:168665/148‑1 (MQ=255)                                              gcGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATc                                                                                                   >  3:346538/1‑149 (MQ=255)                                               cGACTGCAGCAGGCCGCGGGTGAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCt                                                                                                  <  6:235871/149‑1 (MQ=255)                                                   tGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGAt                                                                                              >  6:252598/1‑149 (MQ=255)                                                    gcagcaGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGAt                                                                                              >  6:301134/1‑148 (MQ=255)                                                     cagcagGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGAt                                                                                              >  7:167148/1‑147 (MQ=255)                                                     cagcagGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATga                                                                                            <  5:126667/149‑1 (MQ=255)                                                      agcagGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGAt                                                                                              >  4:256403/1‑146 (MQ=255)                                                       gcagGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATgaga                                                                                          <  3:256403/149‑1 (MQ=255)                                                       gcagGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATgaga                                                                                          >  4:56099/1‑149 (MQ=255)                                                          ggCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCt                                                                                                                      <  6:64623/118‑1 (MQ=255)                                                           gCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTg                                                                                                                     >  7:24769/1‑118 (MQ=255)                                                           gCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTg                                                                                                                     <  8:24769/118‑1 (MQ=255)                                                                gggTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAAt                                                                                 <  5:301134/149‑1 (MQ=255)                                                                      gCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTccc                                                                           <  7:327355/149‑1 (MQ=255)                                                                      gCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTcc                                                                            >  6:317056/1‑148 (MQ=255)                                                                      gCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTcc                                                                            >  8:12243/1‑148 (MQ=255)                                                                        tCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATACGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAg                                                                         >  7:54461/1‑149 (MQ=255)                                                                         ccAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAg                                                                         >  4:107918/1‑148 (MQ=255)                                                                          cAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCt                                                                                                  >  6:68350/1‑122 (MQ=255)                                                                          cAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCt                                                                                                  <  5:68350/122‑1 (MQ=255)                                                                          cAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATga                                                                                            >  7:261783/1‑128 (MQ=255)                                                                          cAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATga                                                                                            <  8:261783/128‑1 (MQ=255)                                                                          cAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGa                                                                       >  6:18855/1‑149 (MQ=255)                                                                           aGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGAGTTGCAGCAACGCGTTCACTTCAGGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGAt                                                                      <  4:215379/149‑1 (MQ=255)                                                                             cgcCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGGTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCTCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATg                                                                     >  8:57167/1‑148 (MQ=255)                                                                              gcCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGaa                                                                   <  7:12243/149‑1 (MQ=255)                                                                               ccAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGc                                                                                    >  1:86043/1‑131 (MQ=255)                                                                               ccAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGc                                                                                    <  2:86043/131‑1 (MQ=255)                                                                                 aGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGcc                                                                >  5:318220/1‑149 (MQ=255)                                                                                  gTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCg                                                               <  7:145919/149‑1 (MQ=255)                                                                                  gTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCATTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATa                                                                                                                >  2:348312/1‑100 (MQ=255)                                                                                  gTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCATTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATa                                                                                                                <  1:348312/100‑1 (MQ=255)                                                                                  gTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCATTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATa                                                                                                                <  3:66759/100‑1 (MQ=255)                                                                                  gTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCATTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATa                                                                                                                >  4:66759/1‑100 (MQ=255)                                                                                    tCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCGAc                                                             >  8:218478/1‑149 (MQ=255)                                                                                       gAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGAt                                                                      >  6:314486/1‑137 (MQ=255)                                                                                       gAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGAt                                                                      <  5:314486/137‑1 (MQ=255)                                                                                         gTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCGACCAGGa                                                        <  6:318220/149‑1 (MQ=255)                                                                                             aTGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCGACCAGGACTGt                                                    <  3:295061/149‑1 (MQ=255)                                                                                                ttgtAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCGACCAGGACTGTCaa                                                 <  7:218478/149‑1 (MQ=255)                                                                                                   tAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCGACCAGGACTGTCAAcc                                               <  2:168224/148‑1 (MQ=255)                                                                                                     ggCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCGACCAGGACTGTCAACCgg                                             <  5:252598/148‑1 (MQ=255)                                                                                                          tccgcgAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCGACCAGGACTGTCAACCGGgcttgc                                       <  5:317056/147‑1 (MQ=255)                                                                                                          tGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCGACCAGGACTGTCAACCGGgcttgc                                       <  3:107918/149‑1 (MQ=255)                                                                                                          tGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCGACCAGGACTGTCAACCGGgcttgc                                       <  7:306480/149‑1 (MQ=255)                                                                                                            cgcgAGGCCGGGTTG‑TGGGTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCTCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAACCCGACCAGGACTGTCAACCGGgcttgctt                                     <  7:57167/149‑1 (MQ=255)                                                                                                               gAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCGACCAGGACTGTCAACCGGGCTTGCTTGTa                                  <  5:18855/149‑1 (MQ=255)                                                                                                                aGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCGACCAGGACTGTCAACCGGGCTTGCTTGTAt                                 <  7:63071/149‑1 (MQ=255)                                                                                                                    cGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAg                                                                         >  5:107736/1‑105 (MQ=255)                                                                                                                                 gATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGtt                                                                              >  5:311510/1‑88 (MQ=255)                                                                                                                                 gATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGtt                                                                              <  6:311510/88‑1 (MQ=255)                                                                                                                                  aTTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCGACCAGGACTGTCAACCGGGCTTGCTTGTATGACAGGCGATTGGTGaa                >  8:240109/1‑149 (MQ=255)                                                                                                                                   tttGAAGAAACGCGTTCACTTCACGGTTGATGGACGCGTCTTAGCTGGCATAATCGATGCCGATTTTTATGAGACTTAGCAATGTTCTTAGGATGAAGCTGATTAGGACTGTTAATCGGGCTTGCTTGTATGACAGGCGATTGGTGaa                <  8:54461/148‑1 (MQ=255)                                                                                                                                       cagcaACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCGACCAGGACTGTCAACCGGGCTTGCTTGTATGACAGGCGATTGGTGAACTTg            <  4:346538/148‑1 (MQ=255)                                                                                                                                        agcaACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCGACCAGGACTGTCAACCGGGCTTGCTTGTATGACAGGCGATTGGTGAACTTGa           >  1:328731/1‑148 (MQ=255)                                                                                                                                          caACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCGACCAGGACTGTCAACCGGGCTTGCTTGTATGACAGGCGATTGGTGAACTTGatat        >  1:321002/1‑149 (MQ=255)                                                                                                                                                gTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCGACCAGGACTGTCAACCGGGCTTGCTTGTATGACAGGCGATTGGTGAACTTGATATCCATGa  <  2:321002/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     GTACCGTTTCGTTGTTGTCGATCAGCCGTGCGCGGATGACGGCCGGCGACTGCAGCAGGCCGCGGGTCAGCTCCAGCGCCAGTTCCGAGTCGATGTTGTAGGCGATGCGCGAGGCCGGGTTG‑TGGCTGATTTGCAGCAACGCGTTCACTTCACGGTTGATGGACGCGTCTTCGCTGGCATAATCGATGCCGATCTGGATGAGACTGAGCAATGTTCCCAGGATGAAGCCGACCAGGACTGTCAACCGGGCTTGCTTGTATGACAGGCGATTGGTGAACTTGATATCCATGA  >  NC_002947/2099559‑2099849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑