Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,104,906	T→G	7.1%	intergenic (‑23/+18)	PP_1879 ← / ← PP_1880	hypothetical protein/outer membrane autotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,104,906	0	T	G	7.1%	163.0 / 2.3	54	intergenic (‑23/+18)	PP_1879/PP_1880	hypothetical protein/outer membrane autotransporter
Reads supporting (aligned to +/- strand):  ref base T (28/22);  new base G (2/2);  total (30/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGACCGCCACCCCTGCACCGATTTCA  >  NC_002947/2104759‑2105053                                                                                                                                                    |                                                                                                                                                     aCATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTg                                                                                                                                                     >  1:234765/1‑149 (MQ=255) aCATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTg                                                                                                                                                     <  2:234765/149‑1 (MQ=255)    tCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGcc                                                                                                                                                  <  2:81054/149‑1 (MQ=255)          accacGCTTGGTACGGTTGCGGGGACAAGAGAGCCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCt                                                                                                                                            >  6:262362/1‑149 (MQ=255)             acGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGt                                                                                                                                         >  4:153250/1‑149 (MQ=255)                cTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTc                                                                                                                                      <  8:188844/149‑1 (MQ=255)                 ttGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTc                                                                                                                                               >  4:47507/1‑139 (MQ=255)                 ttGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTc                                                                                                                                               <  3:47507/139‑1 (MQ=255)                   ggTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCt                                                                                                                                     <  5:343660/147‑1 (MQ=255)                   ggTACGGTTGGGGGGACAAGAGAGGCCGAGACCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCtt                                                                                                                                    >  1:30726/1‑148 (MQ=255)                    gTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTa                                                                                                                                  <  3:152885/149‑1 (MQ=255)                                      gagagGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGcc                                                                                                                >  8:300240/1‑149 (MQ=255)                                      gagagGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGGTTCTTTACCAGTTGTAACGGGCGcc                                                                                                                >  8:271801/1‑149 (MQ=255)                                       agagGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCAAGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGcc                                                                                                                >  4:97241/1‑148 (MQ=255)                                           gCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACg                                                                                                                      >  5:210371/1‑138 (MQ=255)                                           gCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACg                                                                                                                      <  6:210371/138‑1 (MQ=255)                                             cGAGTCCAGTTCTGGGGTTGGAGTTCTGCTGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTc                                                                                                        <  3:97241/149‑1 (MQ=255)                                               aGTCCAGTTCAGGGGTTGGAGTTGTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGTAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCAc                                                                                                      <  7:198911/149‑1 (MQ=255)                                               aGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCAc                                                                                                      <  3:153250/149‑1 (MQ=255)                                                   cAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGccc                                                                                                  <  7:185893/149‑1 (MQ=255)                                                             gTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGtt                                                                                        >  7:216911/1‑149 (MQ=255)                                                                ggAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAACGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCa                                                                                      <  2:80454/148‑1 (MQ=255)                                                                          ggTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGcc                                                                           <  1:155054/149‑1 (MQ=255)                                                                               ccGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGc                                                                      <  8:216911/149‑1 (MQ=255)                                                                               ccGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTAACAGTTGAAACGGGCGCCGAC‑GCTCACGCCCCAAGGCTGATCAAGATTGCTGCCATgg                                                                       >  4:9190/1‑148 (MQ=255)                                                                                  gAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTg                                                                    >  2:164552/1‑148 (MQ=255)                                                                                          gTTCTGTGTCATCGTCCATCTCGATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTTACGGGCGCCGAC‑GCTGACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGaa                                                            <  5:262362/148‑1 (MQ=255)                                                                                          gTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGaa                                                            <  2:30726/148‑1 (MQ=255)                                                                                            tCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCTATGGCTGTAATCGAAGTc                                                         >  3:170609/1‑149 (MQ=255)                                                                                                tgtCATCGTCCTTCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGTCTGTAATCGAAGTCGGc                                                      <  3:9190/148‑1 (MQ=255)                                                                                                     tCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGc                                                >  1:228904/1‑149 (MQ=255)                                                                                                       gTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCt                                              >  6:34985/1‑149 (MQ=255)                                                                                                             ctcCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGcc                                                                           <  5:365769/114‑1 (MQ=255)                                                                                                             ctcCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGcc                                                                           >  6:365769/1‑114 (MQ=255)                                                                                                               ccATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGTGCGCCGAC‑GCTCACGCCCAATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCAtttt                                      >  5:142184/1‑149 (MQ=255)                                                                                                               ccATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCAtttt                                      >  6:69190/1‑149 (MQ=255)                                                                                                               ccATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTAAAGCTTGCTGCCATGGCTGTAATCGAAGTCGCCGTGGGCCTGCCAtttt                                      >  1:288203/1‑149 (MQ=255)                                                                                                                    tgtCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCTATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGcc                                 <  4:170609/149‑1 (MQ=255)                                                                                                                    tgtCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGc                                  <  7:56107/148‑1 (MQ=255)                                                                                                                         cGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATgg                                                                       >  7:210885/1‑106 (MQ=255)                                                                                                                         cGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATgg                                                                       <  8:210885/106‑1 (MQ=255)                                                                                                                            aTGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGa                         >  3:101273/1‑149 (MQ=255)                                                                                                                                aTGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTtg                                                                                >  4:23620/1‑90 (MQ=255)                                                                                                                                aTGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTtg                                                                                <  3:23620/90‑1 (MQ=255)                                                                                                                                 tGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGACCGc                     >  6:334760/1‑148 (MQ=255)                                                                                                                                   cAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGACAGCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGACCGCCa                   >  1:103179/1‑149 (MQ=255)                                                                                                                                   cAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGACCGCCAc                  >  4:42242/1‑149 (MQ=255)                                                                                                                                   cAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACCGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTAGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGACCGCCAc                  >  7:281540/1‑149 (MQ=255)                                                                                                                                       agggaaggCCAATGCGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGACCGcc                    >  4:52198/1‑143 (MQ=255)                                                                                                                                       agggaaggCCAATGCGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGACCGcc                    <  3:52198/143‑1 (MQ=255)                                                                                                                                        gggaaggCCAATGCGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGAc                        <  1:256463/138‑1 (MQ=255)                                                                                                                                        gggaaggCCAATGCGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGAc                        >  2:256463/1‑138 (MQ=255)                                                                                                                                         ggaaggCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGACCGCCACCCCTg             >  5:182826/1‑148 (MQ=255)                                                                                                                                              gcgcATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGACCGCCACCCCTGCACCGa       >  4:318855/1‑149 (MQ=255)                                                                                                                                                   ttGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGACCGCCACCCCTGCACCGATTTCa  <  5:34985/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     ACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGTTACCGGAGCGCTTGTTCTGTGTCATCGTCCATCTCCATGTGTCACGCATGAATGCAGAAGGGAAGGCGCATTGGCCTTCCCTCGTTTCTTTACCAGTTGTAACGGGCGCCGAC‑GCTCACGCCCCATGGCTGTTCAAGCTTGCTGCCATGGCTGTAATCGAAGTCGGCGTGGGCCTGCCATTTTTCGCCCCAGGCGACCGCCACCCCTGCACCGATTTCA  >  NC_002947/2104759‑2105053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑