Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,112,732	C→A	6.0%	P518P (CCG→CCT) ‡	PP_1882 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,112,732	0	C	A	6.0%	239.8 / 3.2	76	P518P (CCG→CCT) ‡	PP_1882	membrane protein
Reads supporting (aligned to +/- strand):  ref base C (38/33);  new base A (3/2);  total (41/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

AGAGGGTCTAGCAGTGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCGGGGT  >  NC_002947/2112586‑2112878                                                                                                                                                   |                                                                                                                                                   agagGGTCTAGCAGTGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCg                                                                                                                                                   >  2:47519/1‑148 (MQ=255)       tCTAGCAGTGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTg                                                                                                                                            >  6:85915/1‑149 (MQ=255)        cTAGCAGTGATTTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACGTCCTTATAGCTACGGCCGTGCCGCATGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTgc                                                                                                                                           <  5:326759/149‑1 (MQ=255)            cAGTGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGCGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCAt                                                                                                                                       <  7:2078/149‑1 (MQ=255)              gTGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGAATCGTGCGCATGc                                                                                                                                     >  1:124397/1‑149 (MQ=255)              atctaTTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGTATGCGCACGATCCGGCGCAtcc                                                                                                                                     <  7:53311/145‑3 (MQ=255)               tGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTCATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCg                                                                                                                                    >  5:369866/1‑149 (MQ=255)                   ttGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCACGATCCGGCGCAtcc                                                                                                                                     >  8:53311/1‑142 (MQ=255)                      aaCTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTgc                                                                                                                                           <  4:115860/135‑1 (MQ=255)                      aaCTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTgc                                                                                                                                           >  3:115860/1‑135 (MQ=255)                      aaCTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGt                                                                                                                             >  7:320553/1‑149 (MQ=255)                      aaCTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGACAGGGt                                                                                                                             >  8:290273/1‑149 (MQ=255)                        cTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGttt                                                                                                                           <  1:47519/149‑1 (MQ=255)                        cTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGttt                                                                                                                           <  4:264247/149‑1 (MQ=255)                           gTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCa                                                                                                                                 >  8:168314/1‑140 (MQ=255)                           gTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCa                                                                                                                                 <  7:168314/140‑1 (MQ=255)                             aCTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACtg                                                                                                                      >  7:339853/1‑149 (MQ=255)                                 ggtggtggtTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGATGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGc                                                                                                                   <  6:95431/148‑1 (MQ=255)                                      tggtTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCACGATCCGGCGCAtcccct                                                                                                                                  <  3:300648/128‑6 (MQ=255)                                      tggtTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCACGATCCGGCGCAtcccct                                                                                                                                  >  4:300648/1‑123 (MQ=255)                                        gtTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACaa                                                                                                            >  2:165761/1‑148 (MQ=255)                                        gtTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACaa                                                                                                            >  2:160250/1‑148 (MQ=255)                                            ccAGCTTGTTGCGTATCACCAGCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAatcat                                                                                                        <  6:369866/148‑1 (MQ=255)                                             cAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAatcatc                                                                                                       <  5:85915/148‑1 (MQ=255)                                                     tgCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAgg                                                                                              >  6:312150/1‑149 (MQ=255)                                                      gCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGACAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAgg                                                                                              <  7:290273/148‑1 (MQ=255)                                                        gTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGt                                                                                            >  7:180878/1‑148 (MQ=255)                                                         tttCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAatcat                                                                                                        >  5:282034/1‑135 (MQ=255)                                                         tttCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAatcat                                                                                                        <  6:282034/135‑1 (MQ=255)                                                               caccTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCAt                                                                                                                                       <  7:336231/98‑1 (MQ=255)                                                               caccTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCAt                                                                                                                                       >  8:336231/1‑98 (MQ=255)                                                                  ctcctGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgc                                                                                 <  7:42120/149‑1 (MQ=255)                                                                  ctcctGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgc                                                                                 <  1:165761/149‑1 (MQ=255)                                                                  ctcctGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgc                                                                                 <  1:160250/149‑1 (MQ=255)                                                                    cctGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATAGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgcat                                                                               >  5:358055/1‑147 (MQ=255)                                                                     ctGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgcgtg                                                                              >  3:44591/1‑149 (MQ=255)                                                                        aTAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgcctgc                                                                             >  2:282341/1‑147 (MQ=255)                                                                           gCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGATAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTg                                                                        >  3:189720/1‑149 (MQ=255)                                                                            cGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTgg                                                                       >  6:248987/1‑149 (MQ=255)                                                                             gACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGgc                                                                      <  7:123746/149‑1 (MQ=255)                                                                                ggCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGATAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGcc                                                                   <  4:189720/149‑1 (MQ=255)                                                                                   cGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAgg                                                                                              >  4:3898/1‑119 (MQ=255)                                                                                   cGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAgg                                                                                              <  3:3898/119‑1 (MQ=255)                                                                                       ccGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGcc                                                             <  5:248987/148‑1 (MQ=255)                                                                                       ccGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGcc                                                             <  3:104114/148‑1 (MQ=255)                                                                                       ccGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCt                                                            <  2:109813/149‑1 (MQ=255)                                                                                        cGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCtt                                                           >  2:56094/1‑149 (MQ=255)                                                                                                 gACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTa                                                  >  4:246679/1‑149 (MQ=255)                                                                                                    ttGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCTGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTg                                               >  3:318569/1‑149 (MQ=255)                                                                                                    ttGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTg                                               >  4:113819/1‑149 (MQ=255)                                                                                                    ttGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTg                                               >  6:46658/1‑149 (MQ=255)                                                                                                      gggCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGc                                              <  8:339853/148‑1 (MQ=255)                                                                                                        gcgcATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCtga                                           <  5:46658/149‑1 (MQ=255)                                                                                                          gcATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCtgagt                                         >  5:244578/1‑149 (MQ=255)                                                                                                          gcATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCtgagt                                         <  5:172778/149‑1 (MQ=255)                                                                                                             tCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCtgagtgag                                      <  3:246679/149‑1 (MQ=255)                                                                                                                aGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCCGAGTGAGAAc                                   <  1:56094/149‑1 (MQ=255)                                                                                                                 ggTAATAGTAAATTTTCTCTTCCAGGGGATGTGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGaa                                    >  4:16211/1‑147 (MQ=255)                                                                                                                  gTAATTGAATATTTTCTCTTCCAGGGGATGCGCAGGAACGTGAGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAg                                 >  2:330216/1‑149 (MQ=255)                                                                                                                  gTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGt                                                >  7:78034/1‑134 (MQ=255)                                                                                                                  gTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGt                                                <  8:78034/134‑1 (MQ=255)                                                                                                                      tAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGc                             <  2:124397/149‑1 (MQ=255)                                                                                                                        gTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGa                                                                                                <  2:188442/80‑1 (MQ=255)                                                                                                                        gTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTACACTGTGCTCGACAATCATCGGGTTGa                                                                                                >  1:188442/1‑80 (MQ=255)                                                                                                                           aaTTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGcc                        >  3:31476/1‑149 (MQ=255)                                                                                                                                tctcTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCg                    <  6:244578/148‑1 (MQ=255)                                                                                                                                  tctTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAggg                               <  3:281294/135‑1 (MQ=255)                                                                                                                                  tctTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAggg                               >  4:281294/1‑135 (MQ=255)                                                                                                                                    ttCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCtt                           >  3:173353/1‑137 (MQ=255)                                                                                                                                    ttCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCtt                           <  4:173353/137‑1 (MQ=255)                                                                                                                                    ttCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCgc               >  7:332833/1‑149 (MQ=255)                                                                                                                                      ccAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCgctg             >  4:104286/1‑149 (MQ=255)                                                                                                                                       cAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCgctgc            >  8:315652/1‑149 (MQ=255)                                                                                                                                           ggATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGc        >  3:343893/1‑149 (MQ=255)                                                                                                                                               gcgcCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCggg    >  6:221965/1‑149 (MQ=255)                                                                                                                                                 gcCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCGGggt  <  4:31476/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   AGAGGGTCTAGCAGTGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCGGGGT  >  NC_002947/2112586‑2112878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑