Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,112,733 G→C 5.3% P518R (CCG→CGG) ‡ PP_1882 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,112,7330GC 5.3% 243.8 / 3.5 75P518R (CCG→CGG) ‡PP_1882membrane protein
Reads supporting (aligned to +/- strand):  ref base G (38/33);  new base C (2/2);  total (40/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.36e-01

AGAGGGTCTAGCAGTGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCGGGGTGGT  >  NC_002947/2112586‑2112881
                                                                                                                                                   |                                                                                                                                                    
agagGGTCTAGCAGTGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCg                                                                                                                                                      >  2:47519/1‑148 (MQ=255)
      tCTAGCAGTGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTg                                                                                                                                               >  6:85915/1‑149 (MQ=255)
       cTAGCAGTGATTTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACGTCCTTATAGCTACGGCCGTGCCGCATGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTgc                                                                                                                                              <  5:326759/149‑1 (MQ=255)
           cAGTGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGCGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCAt                                                                                                                                          <  7:2078/149‑1 (MQ=255)
             gTGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGAATCGTGCGCATGc                                                                                                                                        >  1:124397/1‑149 (MQ=255)
             atctaTTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGTATGCGCACGATCCGGCGCAtcc                                                                                                                                        <  7:53311/145‑3 (MQ=255)
              tGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTCATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCg                                                                                                                                       >  5:369866/1‑149 (MQ=255)
                  ttGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCACGATCCGGCGCAtcc                                                                                                                                        >  8:53311/1‑142 (MQ=255)
                     aaCTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTgc                                                                                                                                              <  4:115860/135‑1 (MQ=255)
                     aaCTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTgc                                                                                                                                              >  3:115860/1‑135 (MQ=255)
                     aaCTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGt                                                                                                                                >  7:320553/1‑149 (MQ=255)
                     aaCTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGACAGGGt                                                                                                                                >  8:290273/1‑149 (MQ=255)
                       cTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGttt                                                                                                                              <  4:264247/149‑1 (MQ=255)
                       cTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGttt                                                                                                                              <  1:47519/149‑1 (MQ=255)
                          gTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCa                                                                                                                                    >  8:168314/1‑140 (MQ=255)
                          gTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCa                                                                                                                                    <  7:168314/140‑1 (MQ=255)
                            aCTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACtg                                                                                                                         >  7:339853/1‑149 (MQ=255)
                                ggtggtggtTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGATGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGc                                                                                                                      <  6:95431/148‑1 (MQ=255)
                                     tggtTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCACGATCCGGCGCAtcccct                                                                                                                                     >  4:300648/1‑123 (MQ=255)
                                     tggtTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCACGATCCGGCGCAtcccct                                                                                                                                     <  3:300648/128‑6 (MQ=255)
                                       gtTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACaa                                                                                                               >  2:165761/1‑148 (MQ=255)
                                       gtTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACaa                                                                                                               >  2:160250/1‑148 (MQ=255)
                                           ccAGCTTGTTGCGTATCACCAGCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAatcat                                                                                                           <  6:369866/148‑1 (MQ=255)
                                            cAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAatcatc                                                                                                          <  5:85915/148‑1 (MQ=255)
                                                    tgCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAgg                                                                                                 >  6:312150/1‑149 (MQ=255)
                                                     gCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGACAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAgg                                                                                                 <  7:290273/148‑1 (MQ=255)
                                                       gTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGt                                                                                               >  7:180878/1‑148 (MQ=255)
                                                        tttCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAatcat                                                                                                           <  6:282034/135‑1 (MQ=255)
                                                        tttCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAatcat                                                                                                           >  5:282034/1‑135 (MQ=255)
                                                              caccTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCAt                                                                                                                                          <  7:336231/98‑1 (MQ=255)
                                                              caccTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCAt                                                                                                                                          >  8:336231/1‑98 (MQ=255)
                                                                 ctcctGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgc                                                                                    <  7:42120/149‑1 (MQ=255)
                                                                 ctcctGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgc                                                                                    <  1:165761/149‑1 (MQ=255)
                                                                 ctcctGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgc                                                                                    <  1:160250/149‑1 (MQ=255)
                                                                   cctGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATAGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgcat                                                                                  >  5:358055/1‑147 (MQ=255)
                                                                    ctGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgcgtg                                                                                 >  3:44591/1‑149 (MQ=255)
                                                                       aTAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgcctgc                                                                                >  2:282341/1‑147 (MQ=255)
                                                                          gCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGATAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTg                                                                           >  3:189720/1‑149 (MQ=255)
                                                                           cGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTgg                                                                          >  6:248987/1‑149 (MQ=255)
                                                                            gACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGgc                                                                         <  7:123746/149‑1 (MQ=255)
                                                                               ggCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGATAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGcc                                                                      <  4:189720/149‑1 (MQ=255)
                                                                                  cGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAgg                                                                                                 >  4:3898/1‑119 (MQ=255)
                                                                                  cGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAgg                                                                                                 <  3:3898/119‑1 (MQ=255)
                                                                                      ccGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGcc                                                                <  5:248987/148‑1 (MQ=255)
                                                                                      ccGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGcc                                                                <  3:104114/148‑1 (MQ=255)
                                                                                      ccGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCt                                                               <  2:109813/149‑1 (MQ=255)
                                                                                       cGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCtt                                                              >  2:56094/1‑149 (MQ=255)
                                                                                                gACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTa                                                     >  4:246679/1‑149 (MQ=255)
                                                                                                   ttGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCTGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTg                                                  >  3:318569/1‑149 (MQ=255)
                                                                                                   ttGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTg                                                  >  6:46658/1‑149 (MQ=255)
                                                                                                   ttGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTg                                                  >  4:113819/1‑149 (MQ=255)
                                                                                                     gggCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGc                                                 <  8:339853/148‑1 (MQ=255)
                                                                                                       gcgcATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCtga                                              <  5:46658/149‑1 (MQ=255)
                                                                                                         gcATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCtgagt                                            >  5:244578/1‑149 (MQ=255)
                                                                                                         gcATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCtgagt                                            <  5:172778/149‑1 (MQ=255)
                                                                                                            tCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCtgagtgag                                         <  3:246679/149‑1 (MQ=255)
                                                                                                               aGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCCGAGTGAGAAc                                      <  1:56094/149‑1 (MQ=255)
                                                                                                                ggTAATAGTAAATTTTCTCTTCCAGGGGATGTGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGaa                                       >  4:16211/1‑147 (MQ=255)
                                                                                                                 gTAATTGAATATTTTCTCTTCCAGGGGATGCGCAGGAACGTGAGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAg                                    >  2:330216/1‑149 (MQ=255)
                                                                                                                 gTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGt                                                   >  7:78034/1‑134 (MQ=255)
                                                                                                                 gTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGt                                                   <  8:78034/134‑1 (MQ=255)
                                                                                                                     tAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGc                                <  2:124397/149‑1 (MQ=255)
                                                                                                                       gTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGa                                                                                                   <  2:188442/80‑1 (MQ=255)
                                                                                                                       gTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTACACTGTGCTCGACAATCATCGGGTTGa                                                                                                   >  1:188442/1‑80 (MQ=255)
                                                                                                                          aaTTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGcc                           >  3:31476/1‑149 (MQ=255)
                                                                                                                               tctcTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCg                       <  6:244578/148‑1 (MQ=255)
                                                                                                                                 tctTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAggg                                  >  4:281294/1‑135 (MQ=255)
                                                                                                                                 tctTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAggg                                  <  3:281294/135‑1 (MQ=255)
                                                                                                                                   ttCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCtt                              <  4:173353/137‑1 (MQ=255)
                                                                                                                                   ttCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCtt                              >  3:173353/1‑137 (MQ=255)
                                                                                                                                   ttCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCgc                  >  7:332833/1‑149 (MQ=255)
                                                                                                                                     ccAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCgctg                >  4:104286/1‑149 (MQ=255)
                                                                                                                                      cAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCgctgc               >  8:315652/1‑149 (MQ=255)
                                                                                                                                          ggATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGc           >  3:343893/1‑149 (MQ=255)
                                                                                                                                              gcgcCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCggg       >  6:221965/1‑149 (MQ=255)
                                                                                                                                                gcCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCGGggt     <  4:31476/149‑1 (MQ=255)
                                                                                                                                                   ggATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCGGggtggt  <  1:282341/149‑1 (MQ=255)
                                                                                                                                                   ggATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCGGggtggt  <  5:221965/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGAGGGTCTAGCAGTGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCGGGGTGGT  >  NC_002947/2112586‑2112881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑