Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,112,739	T→G	5.0%	H516P (CAC→CCC) ‡	PP_1882 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,112,739	0	T	G	5.0%	255.6 / 3.3	79	H516P (CAC→CCC) ‡	PP_1882	membrane protein
Reads supporting (aligned to +/- strand):  ref base T (38/37);  new base G (2/2);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TCTAGCAGTGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCGGGGTGGTGGTTT  >  NC_002947/2112592‑2112886                                                                                                                                                    |                                                                                                                                                    tCTAGCAGTGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTg                                                                                                                                                    >  6:85915/1‑149 (MQ=255)  cTAGCAGTGATTTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACGTCCTTATAGCTACGGCCGTGCCGCATGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTgc                                                                                                                                                   <  5:326759/149‑1 (MQ=255)      cAGTGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGCGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCAt                                                                                                                                               <  7:2078/149‑1 (MQ=255)        gTGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGAATCGTGCGCATGc                                                                                                                                             >  1:124397/1‑149 (MQ=255)        atctaTTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGTATGCGCACGATCCGGCGCAtcc                                                                                                                                             <  7:53311/145‑3 (MQ=255)         tGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTCATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCg                                                                                                                                            >  5:369866/1‑149 (MQ=255)             ttGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCACGATCCGGCGCAtcc                                                                                                                                             >  8:53311/1‑142 (MQ=255)                aaCTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTgc                                                                                                                                                   <  4:115860/135‑1 (MQ=255)                aaCTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTgc                                                                                                                                                   >  3:115860/1‑135 (MQ=255)                aaCTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGt                                                                                                                                     >  7:320553/1‑149 (MQ=255)                aaCTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGACAGGGt                                                                                                                                     >  8:290273/1‑149 (MQ=255)                  cTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGttt                                                                                                                                   <  4:264247/149‑1 (MQ=255)                  cTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGttt                                                                                                                                   <  1:47519/149‑1 (MQ=255)                     gTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCa                                                                                                                                         >  8:168314/1‑140 (MQ=255)                     gTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCa                                                                                                                                         <  7:168314/140‑1 (MQ=255)                       aCTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACtg                                                                                                                              >  7:339853/1‑149 (MQ=255)                           ggtggtggtTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGATGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGc                                                                                                                           <  6:95431/148‑1 (MQ=255)                                tggtTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCACGATCCGGCGCAtcccct                                                                                                                                          >  4:300648/1‑123 (MQ=255)                                tggtTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCACGATCCGGCGCAtcccct                                                                                                                                          <  3:300648/128‑6 (MQ=255)                                  gtTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACaa                                                                                                                    >  2:165761/1‑148 (MQ=255)                                  gtTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACaa                                                                                                                    >  2:160250/1‑148 (MQ=255)                                      ccAGCTTGTTGCGTATCACCAGCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAatcat                                                                                                                <  6:369866/148‑1 (MQ=255)                                       cAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAatcatc                                                                                                               <  5:85915/148‑1 (MQ=255)                                               tgCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAgg                                                                                                      >  6:312150/1‑149 (MQ=255)                                                gCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGACAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAgg                                                                                                      <  7:290273/148‑1 (MQ=255)                                                  gTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGt                                                                                                    >  7:180878/1‑148 (MQ=255)                                                   tttCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAatcat                                                                                                                <  6:282034/135‑1 (MQ=255)                                                   tttCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAatcat                                                                                                                >  5:282034/1‑135 (MQ=255)                                                         caccTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCAt                                                                                                                                               <  7:336231/98‑1 (MQ=255)                                                         caccTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCAt                                                                                                                                               >  8:336231/1‑98 (MQ=255)                                                            ctcctGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgc                                                                                         <  7:42120/149‑1 (MQ=255)                                                            ctcctGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgc                                                                                         <  1:165761/149‑1 (MQ=255)                                                            ctcctGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgc                                                                                         <  1:160250/149‑1 (MQ=255)                                                              cctGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATAGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgcat                                                                                       >  5:358055/1‑147 (MQ=255)                                                               ctGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgcgtg                                                                                      >  3:44591/1‑149 (MQ=255)                                                                  aTAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgcctgc                                                                                     >  2:282341/1‑147 (MQ=255)                                                                     gCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGATAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTg                                                                                >  3:189720/1‑149 (MQ=255)                                                                      cGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTgg                                                                               >  6:248987/1‑149 (MQ=255)                                                                       gACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGgc                                                                              <  7:123746/149‑1 (MQ=255)                                                                          ggCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGATAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGcc                                                                           <  4:189720/149‑1 (MQ=255)                                                                             cGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAgg                                                                                                      >  4:3898/1‑119 (MQ=255)                                                                             cGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAgg                                                                                                      <  3:3898/119‑1 (MQ=255)                                                                                 ccGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGcc                                                                     <  5:248987/148‑1 (MQ=255)                                                                                 ccGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGcc                                                                     <  3:104114/148‑1 (MQ=255)                                                                                 ccGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCt                                                                    <  2:109813/149‑1 (MQ=255)                                                                                  cGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCtt                                                                   >  2:56094/1‑149 (MQ=255)                                                                                           gACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTa                                                          >  4:246679/1‑149 (MQ=255)                                                                                              ttGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCTGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTg                                                       >  3:318569/1‑149 (MQ=255)                                                                                              ttGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTg                                                       >  6:46658/1‑149 (MQ=255)                                                                                              ttGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTg                                                       >  4:113819/1‑149 (MQ=255)                                                                                                gggCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGc                                                      <  8:339853/148‑1 (MQ=255)                                                                                                  gcgcATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCtga                                                   <  5:46658/149‑1 (MQ=255)                                                                                                    gcATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCtgagt                                                 >  5:244578/1‑149 (MQ=255)                                                                                                    gcATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCtgagt                                                 <  5:172778/149‑1 (MQ=255)                                                                                                       tCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCtgagtgag                                              <  3:246679/149‑1 (MQ=255)                                                                                                          aGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCCGAGTGAGAAc                                           <  1:56094/149‑1 (MQ=255)                                                                                                           ggTAATAGTAAATTTTCTCTTCCAGGGGATGTGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGaa                                            >  4:16211/1‑147 (MQ=255)                                                                                                            gTAATTGAATATTTTCTCTTCCAGGGGATGCGCAGGAACGTGAGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAg                                         >  2:330216/1‑149 (MQ=255)                                                                                                            gTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGt                                                        >  7:78034/1‑134 (MQ=255)                                                                                                            gTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGt                                                        <  8:78034/134‑1 (MQ=255)                                                                                                                tAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGc                                     <  2:124397/149‑1 (MQ=255)                                                                                                                  gTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGa                                                                                                        <  2:188442/80‑1 (MQ=255)                                                                                                                  gTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTACACTGTGCTCGACAATCATCGGGTTGa                                                                                                        >  1:188442/1‑80 (MQ=255)                                                                                                                     aaTTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGcc                                >  3:31476/1‑149 (MQ=255)                                                                                                                          tctcTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCg                            <  6:244578/148‑1 (MQ=255)                                                                                                                            tctTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAggg                                       >  4:281294/1‑135 (MQ=255)                                                                                                                            tctTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAggg                                       <  3:281294/135‑1 (MQ=255)                                                                                                                              ttCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCtt                                   >  3:173353/1‑137 (MQ=255)                                                                                                                              ttCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCtt                                   <  4:173353/137‑1 (MQ=255)                                                                                                                              ttCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCgc                       >  7:332833/1‑149 (MQ=255)                                                                                                                                ccAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCgctg                     >  4:104286/1‑149 (MQ=255)                                                                                                                                 cAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCgctgc                    >  8:315652/1‑149 (MQ=255)                                                                                                                                     ggATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGc                >  3:343893/1‑149 (MQ=255)                                                                                                                                         gcgcCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCggg            >  6:221965/1‑149 (MQ=255)                                                                                                                                           gcCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCGGggt          <  4:31476/149‑1 (MQ=255)                                                                                                                                              ggATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCGGggtggt       <  1:282341/149‑1 (MQ=255)                                                                                                                                              ggATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCGGggtggt       <  5:221965/149‑1 (MQ=255)                                                                                                                                                   gTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCGGGGTGGTGGttt  <  3:113819/149‑1 (MQ=255)                                                                                                                                                   gTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCGGGGTGGTGGtt   <  8:180878/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TCTAGCAGTGAATTGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCGGGGTGGTGGTTT  >  NC_002947/2112592‑2112886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑