Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,150,834	T→A	7.3%	noncoding (65/373 nt)	PP_mr28 ←	rne‑II regulatory region

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,150,834	0	T	A	7.3%	196.8 / 4.7	68	noncoding (65/373 nt)	PP_mr28	rne‑II regulatory region
Reads supporting (aligned to +/- strand):  ref base T (33/30);  new base A (2/3);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

CTGCGGTGCCAACAAGGCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTGGTTCTGCGCTGGCGC  >  NC_002947/2150687‑2150980                                                                                                                                                    |                                                                                                                                                   ctgcGGTGCCAACAAGGCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGt                                                                                                                                                    >  6:339199/1‑148 (MQ=255)          caacaaGGCGCCGGAGGCTTCGAATAGGTATCCGTATCAACCAAGCGGGCCTTGTGCAGTGAGTGAGGAGGAGGAATCAACCGTGAGCGGTGGAGTGCTGGTGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTGAGTTGGCtg                                                                                                                                          <  8:261504/149‑1 (MQ=255)          caacaaGGCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCtg                                                                                                                                          >  3:119762/1‑149 (MQ=255)          caacaaGGCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCtg                                                                                                                                          >  7:118946/1‑149 (MQ=255)          caacaaGGCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATACGCTCACCAGCCGTCAGTGGGCtg                                                                                                                                          >  2:72466/1‑149 (MQ=255)           aacaaGGCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCtgg                                                                                                                                         >  6:130253/1‑149 (MQ=255)              aaGGCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAAGCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCCACTGACGGCtggtga                                                                                                                                      <  7:27061/149‑2 (MQ=255)                ggCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGcc                                                                                                                                    <  2:221359/149‑1 (MQ=255)                ggCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGcc                                                                                                                                    <  8:326389/149‑1 (MQ=255)                      ggTGGCTTCGAATAGGTATCAGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGAcgc                                                                                                                              >  8:155697/1‑149 (MQ=255)                           cTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGc                                                                                                                         <  8:118946/149‑1 (MQ=255)                           cTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGC‑CACCAGCCCACTGACGGCTGGTGAGCGGataag                                                                                                                             >  7:14028/1‑140 (MQ=38)                           cTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGC‑CACCAGCCCACTGACGGCTGGTGAGCGGataag                                                                                                                             <  8:14028/144‑5 (MQ=38)                                   aGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGt                                                                                                                 >  5:280529/1‑149 (MQ=255)                                       aTCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTATTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTcc                                                                                                               >  6:303573/1‑147 (MQ=255)                                        tCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAg                                                                                                            >  1:20039/1‑149 (MQ=255)                                        tCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGAAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAg                                                                                                            >  1:253102/1‑149 (MQ=255)                                         ccGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCtgg                                                                                                                                         <  8:291558/119‑1 (MQ=255)                                         ccGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCtgg                                                                                                                                         >  7:291558/1‑119 (MQ=255)                                          cGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAggg                                                                                                          <  5:339199/149‑1 (MQ=255)                                          cGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAgg                                                                                                           <  6:168339/148‑1 (MQ=255)                                            aaTCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCt                                                                                                        <  6:280529/149‑1 (MQ=255)                                                aaCCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCCACTGACGGCtggtgagcg                                                                                                                                   <  6:350518/118‑5 (MQ=255)                                                aaCCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCCACTGACGGCtggtgagcg                                                                                                                                   >  5:350518/1‑114 (MQ=255)                                                aaCCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTATTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCtgtg                                                                                                     <  5:303573/148‑1 (MQ=255)                                                  ccAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCAt                                                                                                  >  3:92859/1‑149 (MQ=255)                                                   cAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCCTCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCAtc                                                                                                 >  5:103352/1‑149 (MQ=255)                                                   cAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCAtc                                                                                                 <  5:130253/149‑1 (MQ=255)                                                         ggCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCt                                                                                                        <  3:62562/136‑1 (MQ=255)                                                         ggCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCt                                                                                                        >  4:62562/1‑136 (MQ=255)                                                               gtgCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGATGGCTGTTCATCTCCACCCTGCa                                                                                      <  8:113042/148‑1 (MQ=255)                                                                       agtcagGTGGAGGATTCAACGGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGAGCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGAGGCGGTGCTACACGGTCCGAGGGGTGTGCATCTCCACCCTGCACGTATccc                                                                              <  4:119762/148‑1 (MQ=255)                                                                       agtcagGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATcc                                                                               <  7:155697/147‑1 (MQ=255)                                                                         tcagGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGa                                                                           <  1:72466/149‑1 (MQ=255)                                                                           aggaggaggAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCAtct                                                                                                >  7:313929/1‑126 (MQ=255)                                                                             gaggaggaATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAAt                                                                       >  3:181655/1‑149 (MQ=255)                                                                                 aggaATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCgg                                                                   <  3:2602/149‑1 (MQ=255)                                                                                  ggaATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCggg                                                                  >  6:238108/1‑149 (MQ=255)                                                                                            gTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCcgcg                                                        >  4:4592/1‑149 (MQ=255)                                                                                               aGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCcgcgcg                                                      <  4:181655/148‑1 (MQ=255)                                                                                                gCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCt                                                    <  5:238108/149‑1 (MQ=255)                                                                                                 ccGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAggg                                                                                                          <  6:136146/94‑1 (MQ=255)                                                                                                 ccGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAggg                                                                                                          >  5:136146/1‑94 (MQ=255)                                                                                                      gACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATc                                               <  2:253102/148‑1 (MQ=255)                                                                                                      gACGCCCGGAGGGCATCTGTTCAGGACCTAATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATcc                                              >  8:8425/1‑149 (MQ=255)                                                                                                         gCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCCTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCAACCTGCACGTATCCCTCATAATTCGGGTGCTGCCGCGCGCTGAATCCGc                                            >  5:81856/1‑148 (MQ=255)                                                                                                           ccGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAAc                                         >  7:223547/1‑149 (MQ=255)                                                                                                            cGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACg                                        <  3:4592/149‑1 (MQ=255)                                                                                                             ggAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACgg                                       <  2:20039/149‑1 (MQ=255)                                                                                                                   cATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCg                                             >  3:299811/1‑137 (MQ=255)                                                                                                                   cATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCg                                             <  4:299811/137‑1 (MQ=255)                                                                                                                    aTCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCCCCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCAt                                >  6:341287/1‑149 (MQ=255)                                                                                                                     tCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCAtt                               >  4:237057/1‑149 (MQ=255)                                                                                                                      cTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCAttt                              >  7:285831/1‑149 (MQ=255)                                                                                                                         ttCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCAtt                               <  7:8425/145‑1 (MQ=255)                                                                                                                         ttCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCAAGTTTCCCTGTTAATTCGGGGGCTGCCGCGCGCTGAATCCGCAACGGGTTGCAttt                              <  6:103352/146‑1 (MQ=255)                                                                                                                                    aTCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTgg                >  2:78814/1‑149 (MQ=255)                                                                                                                                     tCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTgg                <  3:237057/148‑1 (MQ=255)                                                                                                                                        gCTCACCAGCCGTCAATGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTGGTTCt            >  8:223407/1‑149 (MQ=255)                                                                                                                                            aCCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTGGTTCTgcgc        >  6:189752/1‑149 (MQ=255)                                                                                                                                            aCCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGAAACGGGTTGCATTTTTCTCCAGCGCGGGTTCTgcgc        >  6:10442/1‑149 (MQ=255)                                                                                                                                              cAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAgcgc                   >  5:5468/1‑136 (MQ=255)                                                                                                                                              cAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAgcgc                   <  6:5468/136‑1 (MQ=255)                                                                                                                                              cAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTGGTTCTGCGCTg      >  3:253271/1‑149 (MQ=255)                                                                                                                                                gCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTGGTTCTGCGCTGgc    <  8:223547/149‑1 (MQ=255)                                                                                                                                                gCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCAGAACCAGCGCTGgc    <  8:285831/149‑1 (MQ=255)                                                                                                                                                 ccGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGc                         <  5:289328/127‑1 (MQ=255)                                                                                                                                                 ccGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGc                         >  6:289328/1‑127 (MQ=255)                                                                                                                                                   gTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTGGTTCTGCGCTGgcgc  >  5:170550/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                   CTGCGGTGCCAACAAGGCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTGGTTCTGCGCTGGCGC  >  NC_002947/2150687‑2150980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑