Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,150,839	G→C	7.2%	noncoding (60/373 nt)	PP_mr28 ←	rne‑II regulatory region

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,150,839	0	G	C	7.2%	208.2 / 6.8	70	noncoding (60/373 nt)	PP_mr28	rne‑II regulatory region
Reads supporting (aligned to +/- strand):  ref base G (34/30);  new base C (2/3);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-01

CAACAAGGCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTGGTTCTGCGCTGGCGCCATTC  >  NC_002947/2150696‑2150985                                                                                                                                                |                                                                                                                                                   caacaaGGCGCCGGAGGCTTCGAATAGGTATCCGTATCAACCAAGCGGGCCTTGTGCAGTGAGTGAGGAGGAGGAATCAACCGTGAGCGGTGGAGTGCTGGTGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTGAGTTGGCtg                                                                                                                                               <  8:261504/149‑1 (MQ=255) caacaaGGCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCtg                                                                                                                                               >  7:118946/1‑149 (MQ=255) caacaaGGCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCtg                                                                                                                                               >  3:119762/1‑149 (MQ=255) caacaaGGCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATACGCTCACCAGCCGTCAGTGGGCtg                                                                                                                                               >  2:72466/1‑149 (MQ=255)  aacaaGGCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCtgg                                                                                                                                              >  6:130253/1‑149 (MQ=255)     aaGGCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAAGCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCCACTGACGGCtggtga                                                                                                                                           <  7:27061/149‑2 (MQ=255)       ggCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGcc                                                                                                                                         <  2:221359/149‑1 (MQ=255)       ggCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGcc                                                                                                                                         <  8:326389/149‑1 (MQ=255)             ggTGGCTTCGAATAGGTATCAGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGAcgc                                                                                                                                   >  8:155697/1‑149 (MQ=255)                  cTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGc                                                                                                                              <  8:118946/149‑1 (MQ=255)                  cTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGC‑CACCAGCCCACTGACGGCTGGTGAGCGGataag                                                                                                                                  >  7:14028/1‑140 (MQ=38)                  cTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGC‑CACCAGCCCACTGACGGCTGGTGAGCGGataag                                                                                                                                  <  8:14028/144‑5 (MQ=38)                          aGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGt                                                                                                                      >  5:280529/1‑149 (MQ=255)                              aTCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTATTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTcc                                                                                                                    >  6:303573/1‑147 (MQ=255)                               tCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAg                                                                                                                 >  1:20039/1‑149 (MQ=255)                               tCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGAAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAg                                                                                                                 >  1:253102/1‑149 (MQ=255)                                ccGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCtgg                                                                                                                                              >  7:291558/1‑119 (MQ=255)                                ccGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCtgg                                                                                                                                              <  8:291558/119‑1 (MQ=255)                                 cGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAggg                                                                                                               <  5:339199/149‑1 (MQ=255)                                 cGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAgg                                                                                                                <  6:168339/148‑1 (MQ=255)                                   aaTCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCt                                                                                                             <  6:280529/149‑1 (MQ=255)                                       aaCCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCCACTGACGGCtggtgagcg                                                                                                                                        >  5:350518/1‑114 (MQ=255)                                       aaCCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCCACTGACGGCtggtgagcg                                                                                                                                        <  6:350518/118‑5 (MQ=255)                                       aaCCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTATTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCtgtg                                                                                                          <  5:303573/148‑1 (MQ=255)                                         ccAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCAt                                                                                                       >  3:92859/1‑149 (MQ=255)                                          cAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCCTCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCAtc                                                                                                      >  5:103352/1‑149 (MQ=255)                                          cAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCAtc                                                                                                      <  5:130253/149‑1 (MQ=255)                                                ggCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCt                                                                                                             <  3:62562/136‑1 (MQ=255)                                                ggCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCt                                                                                                             >  4:62562/1‑136 (MQ=255)                                                      gtgCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGATGGCTGTTCATCTCCACCCTGCa                                                                                           <  8:113042/148‑1 (MQ=255)                                                              agtcagGTGGAGGATTCAACGGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGAGCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGAGGCGGTGCTACACGGTCCGAGGGGTGTGCATCTCCACCCTGCACGTATccc                                                                                   <  4:119762/148‑1 (MQ=255)                                                              agtcagGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATcc                                                                                    <  7:155697/147‑1 (MQ=255)                                                                tcagGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGa                                                                                <  1:72466/149‑1 (MQ=255)                                                                  aggaggaggAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCAtct                                                                                                     >  7:313929/1‑126 (MQ=255)                                                                    gaggaggaATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAAt                                                                            >  3:181655/1‑149 (MQ=255)                                                                        aggaATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCgg                                                                        <  3:2602/149‑1 (MQ=255)                                                                         ggaATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCggg                                                                       >  6:238108/1‑149 (MQ=255)                                                                                   gTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCcgcg                                                             >  4:4592/1‑149 (MQ=255)                                                                                      aGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCcgcgcg                                                           <  4:181655/148‑1 (MQ=255)                                                                                       gCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCt                                                         <  5:238108/149‑1 (MQ=255)                                                                                        ccGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAggg                                                                                                               >  5:136146/1‑94 (MQ=255)                                                                                        ccGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAggg                                                                                                               <  6:136146/94‑1 (MQ=255)                                                                                             gACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATc                                                    <  2:253102/148‑1 (MQ=255)                                                                                             gACGCCCGGAGGGCATCTGTTCAGGACCTAATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATcc                                                   >  8:8425/1‑149 (MQ=255)                                                                                                gCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCCTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCAACCTGCACGTATCCCTCATAATTCGGGTGCTGCCGCGCGCTGAATCCGc                                                 >  5:81856/1‑148 (MQ=255)                                                                                                  ccGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAAc                                              >  7:223547/1‑149 (MQ=255)                                                                                                   cGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACg                                             <  3:4592/149‑1 (MQ=255)                                                                                                    ggAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACgg                                            <  2:20039/149‑1 (MQ=255)                                                                                                          cATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCg                                                  <  4:299811/137‑1 (MQ=255)                                                                                                          cATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCg                                                  >  3:299811/1‑137 (MQ=255)                                                                                                           aTCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCCCCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCAt                                     >  6:341287/1‑149 (MQ=255)                                                                                                            tCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCAtt                                    >  4:237057/1‑149 (MQ=255)                                                                                                             cTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCAttt                                   >  7:285831/1‑149 (MQ=255)                                                                                                                ttCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCAtt                                    <  7:8425/145‑1 (MQ=255)                                                                                                                ttCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCAAGTTTCCCTGTTAATTCGGGGGCTGCCGCGCGCTGAATCCGCAACGGGTTGCAttt                                   <  6:103352/146‑1 (MQ=255)                                                                                                                           aTCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTgg                     >  2:78814/1‑149 (MQ=255)                                                                                                                            tCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTgg                     <  3:237057/148‑1 (MQ=255)                                                                                                                               gCTCACCAGCCGTCAATGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTGGTTCt                 >  8:223407/1‑149 (MQ=255)                                                                                                                                   aCCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTGGTTCTgcgc             >  6:189752/1‑149 (MQ=255)                                                                                                                                   aCCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGAAACGGGTTGCATTTTTCTCCAGCGCGGGTTCTgcgc             >  6:10442/1‑149 (MQ=255)                                                                                                                                     cAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAgcgc                        >  5:5468/1‑136 (MQ=255)                                                                                                                                     cAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAgcgc                        <  6:5468/136‑1 (MQ=255)                                                                                                                                     cAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTGGTTCTGCGCTg           >  3:253271/1‑149 (MQ=255)                                                                                                                                       gCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTGGTTCTGCGCTGgc         <  8:223547/149‑1 (MQ=255)                                                                                                                                       gCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCAGAACCAGCGCTGgc         <  8:285831/149‑1 (MQ=255)                                                                                                                                        ccGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGc                              <  5:289328/127‑1 (MQ=255)                                                                                                                                        ccGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGc                              >  6:289328/1‑127 (MQ=255)                                                                                                                                          gTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTGGTTCTGCGCTGgcgc       >  5:170550/1‑148 (MQ=255)                                                                                                                                              gTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTGGTTCTGCGCTGGCGCCAtt   >  2:34272/1‑148 (MQ=255)                                                                                                                                              gTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCATGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTGGTTCTGCGCTGGCGCCATTc  <  4:92859/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                   CAACAAGGCCCCGGTGGCTTCGAATAGGTATCCGAATCAACCAAGCGGGCCTTGTGCACTCAGTCAGGAGGAGGAATCAACCGTCAGCCGTGGACGCCCGGAGGGCATCTGTTCAGGACCTTATCCGCTCACCAGCCGTCAGTGGGCTGGTGGCCGGACGCGGTGCTACACGGTCCGAGGGCTGTGCATCTCCACCCTGCACGTATCCCTGATAATTCGGGTGCTGCCGCGCGCTGAATCCGCAACGGGTTGCATTTTTCGCCAGCGCTGGTTCTGCGCTGGCGCCATTC  >  NC_002947/2150696‑2150985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑