Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,155,087 A→G 7.8% S86G (AGC→GGC)  PP_1910 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,155,0870AG 7.8% 182.9 / 4.1 64S86G (AGC→GGC) PP_1910hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (34/25);  new base G (3/2);  total (37/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

GACAATGTCGGTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCT  >  NC_002947/2154949‑2155233
                                                                                                                                          |                                                                                                                                                  
gACAATGTCGGTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTa                                                                                                                                           >  1:96151/1‑148 (MQ=255)
  cattGTCGGTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACAt                                                                                                                                        <  7:122500/146‑1 (MQ=255)
    aTGTCGGTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCAGCCTACCGATCCACAGCGAATGTACATAc                                                                                                                                      >  6:349097/1‑149 (MQ=255)
         ggTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACGGCGTATGTACATTCGCTgt                                                                                                                                 <  8:151874/149‑1 (MQ=255)
         ggTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACGGCGTATGTACATTCGCTgt                                                                                                                                 >  7:151874/1‑149 (MQ=255)
         ggTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCgt                                                                                                                                 >  5:111137/1‑149 (MQ=255)
                tGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAg                                                                                                                                                   >  4:261486/1‑124 (MQ=255)
                tGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAg                                                                                                                                                   <  3:261486/124‑1 (MQ=255)
                     gCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAaggagtg                                                                                                                     <  7:328150/149‑3 (MQ=255)
                     gCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAggg                                                                                                                     >  2:344205/1‑149 (MQ=255)
                            tCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGaa                                                                                                               >  7:248457/1‑148 (MQ=255)
                                               aCAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAg                                                                                           >  8:191391/1‑149 (MQ=255)
                                                 aGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGc                                                                                         <  2:96151/149‑1 (MQ=255)
                                                      gtggtgGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGa                                                                                    >  6:165778/1‑149 (MQ=255)
                                                      gtggtgGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCATACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGa                                                                                    >  2:194770/1‑149 (MQ=255)
                                                        ggtggtTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAg                                                                                                        <  8:114980/127‑1 (MQ=255)
                                                        ggtggtTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAg                                                                                                        >  7:114980/1‑127 (MQ=255)
                                                         gtggtTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGa                                                                                    >  5:288585/1‑146 (MQ=255)
                                                         gtggtTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGa                                                                                    <  6:288585/146‑1 (MQ=255)
                                                            gtTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCAATACTTTGCCGAAAGGCTATGACGTgat                                                                              >  2:89236/1‑147 (MQ=255)
                                                                 ccACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAAc                                                                         >  8:22611/1‑149 (MQ=255)
                                                                  cacaGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAAc                                                                         <  7:281467/148‑1 (MQ=255)
                                                                  cacaGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACt                                                                        >  6:90911/1‑149 (MQ=255)
                                                                   acaGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTg                                                                       >  8:288404/1‑149 (MQ=255)
                                                                   acaGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTg                                                                       >  6:263506/1‑149 (MQ=255)
                                                                     aGCGAGCTGGACGTCGAAGTCGAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATACACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCGGAAAGGCTATGACGTGCTGGAACTggg                                                                     >  3:32217/1‑149 (MQ=255)
                                                                     aGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTggg                                                                     <  6:111137/149‑1 (MQ=255)
                                                                         aGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGaa                                                                 <  5:263506/149‑1 (MQ=255)
                                                                               aCGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCt                                                           >  5:358510/1‑149 (MQ=255)
                                                                                 gTCGAAGTCAAGATGGTTTGCCAGCGTTGTGTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCttt                                                         >  1:30133/1‑149 (MQ=255)
                                                                                    gAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGAc                                                                                   >  5:289135/1‑120 (MQ=255)
                                                                                     aaGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCTAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGa                                                      >  5:82540/1‑148 (MQ=255)
                                                                                        tCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTg                                                  >  4:359633/1‑149 (MQ=255)
                                                                                          aaGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTg                                                  >  8:244027/1‑147 (MQ=255)
                                                                                          aaGATGGTTTGCCAGCGTTGTCTAGAGCTGGTCACCCTACCGATCCACAGCGAATGTAGATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTg                                                  >  7:264437/1‑147 (MQ=255)
                                                                                             aTGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGc                                             <  6:358510/149‑1 (MQ=255)
                                                                                                gTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCAt                                           <  7:288404/148‑1 (MQ=255)
                                                                                                gTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCAt                                           <  7:191391/148‑1 (MQ=255)
                                                                                                      cAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTc                                      >  6:286861/1‑147 (MQ=255)
                                                                                                       aGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCgag                                   <  5:165778/149‑1 (MQ=255)
                                                                                                             gTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCgaggagga                              <  1:344205/148‑1 (MQ=255)
                                                                                                              tCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGc                            >  8:298794/1‑149 (MQ=255)
                                                                                                                ttGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCtt                          >  3:176241/1‑149 (MQ=255)
                                                                                                                  gAGCTGGTCACCCTACCGATCCACATCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCttt                         <  8:248457/148‑1 (MQ=255)
                                                                                                                    gCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCAt                                           <  4:230951/128‑1 (MQ=255)
                                                                                                                    gCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCAt                                           >  3:230951/1‑128 (MQ=255)
                                                                                                                    gCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTg                       <  7:298794/148‑1 (MQ=255)
                                                                                                                    gCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGc                      <  7:244027/149‑1 (MQ=255)
                                                                                                                        gTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGc                  >  8:297862/1‑149 (MQ=255)
                                                                                                                           aCCCTACCGATCCACAGCGAATGTACATACGCCGTGTTGATGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGATGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTc                    <  6:319922/144‑1 (MQ=255)
                                                                                                                           aCCCTACCGATCCACAGCGAATGTACATACGCCGTGGAGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATAGGACGAGGAGGAGCTTTTGCTc                    >  5:319922/1‑144 (MQ=255)
                                                                                                                             ccTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGc             <  3:359633/149‑1 (MQ=255)
                                                                                                                              cTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGc             >  7:271399/1‑148 (MQ=255)
                                                                                                                                 cttcaccACGGCGTATGTACATTCGCTGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAAt         >  5:165021/6‑149 (MQ=255)
                                                                                                                                 ccGATCCACAGCGAATGTTCATACGCCGTGGTTAAGGAGGGTGGGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGATCTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGTAGTAGCTTTTGCTCGCCTTGCCAAt         <  6:82540/149‑1 (MQ=255)
                                                                                                                                 ccGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAAt         <  7:22611/149‑1 (MQ=255)
                                                                                                                                 ccGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAAt         <  8:271399/149‑1 (MQ=255)
                                                                                                                                 ccGATCCACAGCGAATGTACATACGCCGTCGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAAt         >  2:71811/1‑149 (MQ=255)
                                                                                                                                  ttcaccACGGCGTATGTACATTCGCTGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCga                                    <  8:190776/117‑1 (MQ=39)
                                                                                                                                  ttcaccACGGCGTATGTACATTCGCTGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCga                                    >  7:190776/5‑121 (MQ=39)
                                                                                                                                  cGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATc        <  1:194770/149‑1 (MQ=255)
                                                                                                                                    aTCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAg                                               <  2:57238/108‑1 (MQ=255)
                                                                                                                                    aTCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAg                                               >  1:57238/1‑108 (MQ=255)
                                                                                                                                      ccACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGc    >  7:322692/1‑149 (MQ=255)
                                                                                                                                        acaGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCt  >  7:106534/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                  
GACAATGTCGGTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCT  >  NC_002947/2154949‑2155233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑