Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,155,100	A→T	7.7%	Y90F (TAC→TTC)	PP_1910 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,155,100	0	A	T	7.7%	189.5 / 4.9	65	Y90F (TAC→TTC)	PP_1910	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (34/26);  new base T (3/2);  total (37/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

ATGTCGGTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCTCATCATCCGGA  >  NC_002947/2154953‑2155247                                                                                                                                                    |                                                                                                                                                    aTGTCGGTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCAGCCTACCGATCCACAGCGAATGTACATAc                                                                                                                                                    >  6:349097/1‑149 (MQ=255)      ggTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACGGCGTATGTACATTCGCTgt                                                                                                                                               >  7:151874/1‑149 (MQ=255)      ggTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACGGCGTATGTACATTCGCTgt                                                                                                                                               <  8:151874/149‑1 (MQ=255)      ggTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCgt                                                                                                                                               >  5:111137/1‑149 (MQ=255)                  gCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAaggagtg                                                                                                                                   <  7:328150/149‑3 (MQ=255)                  gCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAggg                                                                                                                                   >  2:344205/1‑149 (MQ=255)                         tCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGaa                                                                                                                             >  7:248457/1‑148 (MQ=255)                                            aCAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAg                                                                                                         >  8:191391/1‑149 (MQ=255)                                              aGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGc                                                                                                       <  2:96151/149‑1 (MQ=255)                                                   gtggtgGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGa                                                                                                  >  6:165778/1‑149 (MQ=255)                                                   gtggtgGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCATACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGa                                                                                                  >  2:194770/1‑149 (MQ=255)                                                     ggtggtTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAg                                                                                                                      <  8:114980/127‑1 (MQ=255)                                                     ggtggtTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAg                                                                                                                      >  7:114980/1‑127 (MQ=255)                                                      gtggtTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGa                                                                                                  <  6:288585/146‑1 (MQ=255)                                                      gtggtTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGa                                                                                                  >  5:288585/1‑146 (MQ=255)                                                         gtTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCAATACTTTGCCGAAAGGCTATGACGTgat                                                                                            >  2:89236/1‑147 (MQ=255)                                                              ccACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAAc                                                                                       >  8:22611/1‑149 (MQ=255)                                                               cacaGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAAc                                                                                       <  7:281467/148‑1 (MQ=255)                                                               cacaGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACt                                                                                      >  6:90911/1‑149 (MQ=255)                                                                acaGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTg                                                                                     >  6:263506/1‑149 (MQ=255)                                                                acaGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTg                                                                                     >  8:288404/1‑149 (MQ=255)                                                                  aGCGAGCTGGACGTCGAAGTCGAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATACACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCGGAAAGGCTATGACGTGCTGGAACTggg                                                                                   >  3:32217/1‑149 (MQ=255)                                                                  aGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTggg                                                                                   <  6:111137/149‑1 (MQ=255)                                                                      aGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGaa                                                                               <  5:263506/149‑1 (MQ=255)                                                                            aCGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCt                                                                         >  5:358510/1‑149 (MQ=255)                                                                              gTCGAAGTCAAGATGGTTTGCCAGCGTTGTGTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCttt                                                                       >  1:30133/1‑149 (MQ=255)                                                                                 gAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGAc                                                                                                 >  5:289135/1‑120 (MQ=255)                                                                                  aaGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCTAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGa                                                                    >  5:82540/1‑148 (MQ=255)                                                                                     tCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTg                                                                >  4:359633/1‑149 (MQ=255)                                                                                       aaGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTg                                                                >  8:244027/1‑147 (MQ=255)                                                                                       aaGATGGTTTGCCAGCGTTGTCTAGAGCTGGTCACCCTACCGATCCACAGCGAATGTAGATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTg                                                                >  7:264437/1‑147 (MQ=255)                                                                                          aTGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGc                                                           <  6:358510/149‑1 (MQ=255)                                                                                             gTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCAt                                                         <  7:288404/148‑1 (MQ=255)                                                                                             gTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCAt                                                         <  7:191391/148‑1 (MQ=255)                                                                                                   cAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTc                                                    >  6:286861/1‑147 (MQ=255)                                                                                                    aGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCgag                                                 <  5:165778/149‑1 (MQ=255)                                                                                                          gTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCgaggagga                                            <  1:344205/148‑1 (MQ=255)                                                                                                           tCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGc                                          >  8:298794/1‑149 (MQ=255)                                                                                                             ttGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCtt                                        >  3:176241/1‑149 (MQ=255)                                                                                                               gAGCTGGTCACCCTACCGATCCACATCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCttt                                       <  8:248457/148‑1 (MQ=255)                                                                                                                 gCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCAt                                                         >  3:230951/1‑128 (MQ=255)                                                                                                                 gCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCAt                                                         <  4:230951/128‑1 (MQ=255)                                                                                                                 gCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTg                                     <  7:298794/148‑1 (MQ=255)                                                                                                                 gCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGc                                    <  7:244027/149‑1 (MQ=255)                                                                                                                     gTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGc                                >  8:297862/1‑149 (MQ=255)                                                                                                                        aCCCTACCGATCCACAGCGAATGTACATACGCCGTGTTGATGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGATGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTc                                  <  6:319922/144‑1 (MQ=255)                                                                                                                        aCCCTACCGATCCACAGCGAATGTACATACGCCGTGGAGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATAGGACGAGGAGGAGCTTTTGCTc                                  >  5:319922/1‑144 (MQ=255)                                                                                                                          ccTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGc                           <  3:359633/149‑1 (MQ=255)                                                                                                                           cTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGc                           >  7:271399/1‑148 (MQ=255)                                                                                                                              cttcaccACGGCGTATGTACATTCGCTGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAAt                       >  5:165021/6‑149 (MQ=255)                                                                                                                              ccGATCCACAGCGAATGTTCATACGCCGTGGTTAAGGAGGGTGGGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGATCTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGTAGTAGCTTTTGCTCGCCTTGCCAAt                       <  6:82540/149‑1 (MQ=255)                                                                                                                              ccGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAAt                       <  7:22611/149‑1 (MQ=255)                                                                                                                              ccGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAAt                       <  8:271399/149‑1 (MQ=255)                                                                                                                              ccGATCCACAGCGAATGTACATACGCCGTCGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAAt                       >  2:71811/1‑149 (MQ=255)                                                                                                                               ttcaccACGGCGTATGTACATTCGCTGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCga                                                  <  8:190776/117‑1 (MQ=39)                                                                                                                               ttcaccACGGCGTATGTACATTCGCTGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCga                                                  >  7:190776/5‑121 (MQ=39)                                                                                                                               cGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATc                      <  1:194770/149‑1 (MQ=255)                                                                                                                                 aTCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAg                                                             <  2:57238/108‑1 (MQ=255)                                                                                                                                 aTCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAg                                                             >  1:57238/1‑108 (MQ=255)                                                                                                                                   ccACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGc                  >  7:322692/1‑149 (MQ=255)                                                                                                                                     acaGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCt                >  7:106534/1‑149 (MQ=255)                                                                                                                                          gAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCtca           <  8:264437/149‑1 (MQ=255)                                                                                                                                               tACATACGCCGTTTTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCTAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTTCTcatcat       <  4:32217/148‑1 (MQ=255)                                                                                                                                               tACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCTCatcatc      >  8:41787/1‑149 (MQ=255)                                                                                                                                                   tACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCTCATCATCCGga  <  8:106534/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ATGTCGGTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCTCATCATCCGGA  >  NC_002947/2154953‑2155247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑