Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,155,104 C→T 7.8% A91A (GCC→GCT)  PP_1910 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,155,1040CT 7.8% 179.9 / 4.5 64A91A (GCC→GCT) PP_1910hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (33/26);  new base T (3/2);  total (36/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.65e-01

GGTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCTCATCATCCGGA  >  NC_002947/2154958‑2155247
                                                                                                                                                  |                                                                                                                                               
ggTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACGGCGTATGTACATTCGCTgt                                                                                                                                               >  7:151874/1‑149 (MQ=255)
ggTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACGGCGTATGTACATTCGCTgt                                                                                                                                               <  8:151874/149‑1 (MQ=255)
ggTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCgt                                                                                                                                               >  5:111137/1‑149 (MQ=255)
            gCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAaggagtg                                                                                                                                   <  7:328150/149‑3 (MQ=255)
            gCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAggg                                                                                                                                   >  2:344205/1‑149 (MQ=255)
                   tCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGaa                                                                                                                             >  7:248457/1‑148 (MQ=255)
                                      aCAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAg                                                                                                         >  8:191391/1‑149 (MQ=255)
                                        aGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGc                                                                                                       <  2:96151/149‑1 (MQ=255)
                                             gtggtgGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGa                                                                                                  >  6:165778/1‑149 (MQ=255)
                                             gtggtgGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCATACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGa                                                                                                  >  2:194770/1‑149 (MQ=255)
                                               ggtggtTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAg                                                                                                                      <  8:114980/127‑1 (MQ=255)
                                               ggtggtTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAg                                                                                                                      >  7:114980/1‑127 (MQ=255)
                                                gtggtTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGa                                                                                                  >  5:288585/1‑146 (MQ=255)
                                                gtggtTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGa                                                                                                  <  6:288585/146‑1 (MQ=255)
                                                   gtTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCAATACTTTGCCGAAAGGCTATGACGTgat                                                                                            >  2:89236/1‑147 (MQ=255)
                                                        ccACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAAc                                                                                       >  8:22611/1‑149 (MQ=255)
                                                         cacaGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAAc                                                                                       <  7:281467/148‑1 (MQ=255)
                                                         cacaGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACt                                                                                      >  6:90911/1‑149 (MQ=255)
                                                          acaGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTg                                                                                     >  6:263506/1‑149 (MQ=255)
                                                          acaGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTg                                                                                     >  8:288404/1‑149 (MQ=255)
                                                            aGCGAGCTGGACGTCGAAGTCGAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATACACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCGGAAAGGCTATGACGTGCTGGAACTggg                                                                                   >  3:32217/1‑149 (MQ=255)
                                                            aGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTggg                                                                                   <  6:111137/149‑1 (MQ=255)
                                                                aGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGaa                                                                               <  5:263506/149‑1 (MQ=255)
                                                                      aCGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCt                                                                         >  5:358510/1‑149 (MQ=255)
                                                                        gTCGAAGTCAAGATGGTTTGCCAGCGTTGTGTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCttt                                                                       >  1:30133/1‑149 (MQ=255)
                                                                           gAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGAc                                                                                                 >  5:289135/1‑120 (MQ=255)
                                                                            aaGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCTAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGa                                                                    >  5:82540/1‑148 (MQ=255)
                                                                               tCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTg                                                                >  4:359633/1‑149 (MQ=255)
                                                                                 aaGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTg                                                                >  8:244027/1‑147 (MQ=255)
                                                                                 aaGATGGTTTGCCAGCGTTGTCTAGAGCTGGTCACCCTACCGATCCACAGCGAATGTAGATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTg                                                                >  7:264437/1‑147 (MQ=255)
                                                                                    aTGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGc                                                           <  6:358510/149‑1 (MQ=255)
                                                                                       gTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCAt                                                         <  7:191391/148‑1 (MQ=255)
                                                                                       gTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCAt                                                         <  7:288404/148‑1 (MQ=255)
                                                                                             cAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTc                                                    >  6:286861/1‑147 (MQ=255)
                                                                                              aGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCgag                                                 <  5:165778/149‑1 (MQ=255)
                                                                                                    gTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCgaggagga                                            <  1:344205/148‑1 (MQ=255)
                                                                                                     tCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGc                                          >  8:298794/1‑149 (MQ=255)
                                                                                                       ttGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCtt                                        >  3:176241/1‑149 (MQ=255)
                                                                                                         gAGCTGGTCACCCTACCGATCCACATCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCttt                                       <  8:248457/148‑1 (MQ=255)
                                                                                                           gCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCAt                                                         >  3:230951/1‑128 (MQ=255)
                                                                                                           gCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCAt                                                         <  4:230951/128‑1 (MQ=255)
                                                                                                           gCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTg                                     <  7:298794/148‑1 (MQ=255)
                                                                                                           gCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGc                                    <  7:244027/149‑1 (MQ=255)
                                                                                                               gTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGc                                >  8:297862/1‑149 (MQ=255)
                                                                                                                  aCCCTACCGATCCACAGCGAATGTACATACGCCGTGTTGATGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGATGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTc                                  <  6:319922/144‑1 (MQ=255)
                                                                                                                  aCCCTACCGATCCACAGCGAATGTACATACGCCGTGGAGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATAGGACGAGGAGGAGCTTTTGCTc                                  >  5:319922/1‑144 (MQ=255)
                                                                                                                    ccTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGc                           <  3:359633/149‑1 (MQ=255)
                                                                                                                     cTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGc                           >  7:271399/1‑148 (MQ=255)
                                                                                                                        cttcaccACGGCGTATGTACATTCGCTGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAAt                       >  5:165021/6‑149 (MQ=255)
                                                                                                                        ccGATCCACAGCGAATGTTCATACGCCGTGGTTAAGGAGGGTGGGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGATCTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGTAGTAGCTTTTGCTCGCCTTGCCAAt                       <  6:82540/149‑1 (MQ=255)
                                                                                                                        ccGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAAt                       <  7:22611/149‑1 (MQ=255)
                                                                                                                        ccGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAAt                       <  8:271399/149‑1 (MQ=255)
                                                                                                                        ccGATCCACAGCGAATGTACATACGCCGTCGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAAt                       >  2:71811/1‑149 (MQ=255)
                                                                                                                         ttcaccACGGCGTATGTACATTCGCTGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCga                                                  >  7:190776/5‑121 (MQ=39)
                                                                                                                         ttcaccACGGCGTATGTACATTCGCTGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCga                                                  <  8:190776/117‑1 (MQ=39)
                                                                                                                         cGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATc                      <  1:194770/149‑1 (MQ=255)
                                                                                                                           aTCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAg                                                             >  1:57238/1‑108 (MQ=255)
                                                                                                                           aTCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAg                                                             <  2:57238/108‑1 (MQ=255)
                                                                                                                             ccACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGc                  >  7:322692/1‑149 (MQ=255)
                                                                                                                               acaGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCt                >  7:106534/1‑149 (MQ=255)
                                                                                                                                    gAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCtca           <  8:264437/149‑1 (MQ=255)
                                                                                                                                         tACATACGCCGTTTTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCTAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTTCTcatcat       <  4:32217/148‑1 (MQ=255)
                                                                                                                                         tACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCTCatcatc      >  8:41787/1‑149 (MQ=255)
                                                                                                                                             tACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCTCATCATCCGga  <  8:106534/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GGTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCTCATCATCCGGA  >  NC_002947/2154958‑2155247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑