Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,178,524 G→A 7.5% A254T (GCC→ACC)  PP_1931 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,178,5240GA 7.5% 148.5 / 2.5 53A254T (GCC→ACC) PP_1931hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (30/19);  new base A (2/2);  total (32/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01

GCTGAAACAACAGATGCAGAAGCTCAAAGATATTGAAACGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAACATGGCGAAGCAAGCGCGTGACCAAATCGGTGCTGAATCGCTGACGGTCGTTGCCGACCGGGGCTAT  >  NC_002947/2178376‑2178667
                                                                                                                                                    |                                                                                                                                               
gCTGAAACAACAGATGCAGAAGCTCAAAGATATTGAAACGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACgg                                                                                                                                                 <  1:7193/149‑1 (MQ=255)
gCTGAAACAACAGATGCAGAAGCTCAAAGATATTGAAACGCAGCTACTTCAAAACCCAGACAAACAAATCTCTCTCACAGATACCGATGCCCTCTCCATCTCAGATGGCGGTCGGGGCAGTGGCACCGTAGGCTATAAAGTAAAATCgg                                                                                                                                                 >  8:272684/1‑149 (MQ=255)
       caacaGATGCAGAAGCTCAAAGATATTGAAACGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGa                                                                                                                                          <  1:363519/149‑1 (MQ=255)
        aacaGATGCAGAAGCTCTAAGATATTGAAACGCATCTACTTCAAACCCCAGACTTACAAATCTGTCTCTCAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGAc                                                                                                                                         <  7:272684/149‑1 (MQ=255)
        aacaGATGCAGAAGCTCAAAGATATTGAAACGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGAc                                                                                                                                         <  6:165760/149‑1 (MQ=255)
            gATGCAGAAGCTCAAAGATATTGAAACGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGAt                                                                                                                                      >  7:206173/1‑148 (MQ=255)
             aTGCAGAAGCTCAAAGATATTGAAACGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGAt                                                                                                                                      >  2:30273/1‑147 (MQ=255)
               gCAGAAGCTCAAAGATATTGAAACGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATc                                                                                                                                     >  1:326114/1‑146 (MQ=255)
                cAGAAGCTCAAAGATATTGAAACGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGc                                                                                                                                  <  3:219969/148‑1 (MQ=255)
                        cAAAGATATTGAAACGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATc                                                                                                                                     <  2:11751/137‑1 (MQ=255)
                        cAAAGATATTGAAACGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATc                                                                                                                                     >  1:11751/1‑137 (MQ=255)
                         aaaGATATTGAAACGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCTCCGTTGGCTATAAGGTACAAAGGGCGGTTGACGATCCGCACCATCGGAt                                                                                                                        >  8:121698/1‑149 (MQ=255)
                          aaGATATTGAAACGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATc                                                                                                                       <  1:30273/149‑1 (MQ=255)
                                  gAAACGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCa                                                                                                               >  4:116755/1‑149 (MQ=255)
                                   aaaCGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGACCGTTGACGATCAGCACCATc                                                                                                                            >  5:171726/1‑135 (MQ=255)
                                   aaaCGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGACCGTTGACGATCAGCACCATc                                                                                                                            >  5:176791/1‑135 (MQ=255)
                                   aaaCGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGACCGTTGACGATCAGCACCATc                                                                                                                            <  6:176791/135‑1 (MQ=255)
                                   aaaCGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGACCGTTGACGATCAGCACCATc                                                                                                                            <  6:171726/135‑1 (MQ=255)
                                         aGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTg                                                                                                        <  3:76417/149‑1 (MQ=255)
                                          gCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGa                                                                                                       >  2:13060/1‑149 (MQ=255)
                                            tACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGAc                                                                                                      >  6:194753/1‑148 (MQ=255)
                                             aCTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACg                                                                                                     >  3:299147/1‑148 (MQ=255)
                                                 cAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGt                                                                                                >  8:143075/1‑149 (MQ=255)
                                                           acaaacaaATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGAt                                                                                      >  6:127532/1‑149 (MQ=255)
                                                              aacaaaTCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATc                                                                                                                            <  6:124791/108‑1 (MQ=255)
                                                              aacaaaTCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCACCACCATc                                                                                                                            >  5:124791/1‑108 (MQ=255)
                                                               acaaaTCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATcgcg                                                                                  <  1:13060/149‑1 (MQ=255)
                                                                 aaaTCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGc                                                                                >  1:327171/1‑149 (MQ=255)
                                                                 aaaTCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGc                                                                                <  5:194753/149‑1 (MQ=255)
                                                                       ctctcACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGtt                                                                           >  7:45894/1‑148 (MQ=255)
                                                                        tctcACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGTGGCAGTGGCACCGTTGGCTATAACGTACAAAGGGCAGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGa                                                                         <  3:113945/149‑1 (MQ=255)
                                                                          tcACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAg                                                                        >  2:2335/1‑148 (MQ=255)
                                                                                    cGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCa                                                                                                                      <  6:191559/92‑1 (MQ=255)
                                                                                    cGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCa                                                                                                                      >  5:191559/1‑92 (MQ=255)
                                                                                            gCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAACATGGCGAAGCAAgcg                                                     >  5:204884/1‑149 (MQ=255)
                                                                                                cATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCAt                                                                                         <  5:110977/109‑1 (MQ=255)
                                                                                                cATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCAt                                                                                         >  6:110977/1‑109 (MQ=255)
                                                                                                    tCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAACATGGCGAAGCAAGCGCGTGAcc                                              >  2:354015/1‑148 (MQ=255)
                                                                                                           gCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAACATGGCGAAGCAAGCGCGTGACCAAATCGGt                                      >  4:36564/1‑149 (MQ=255)
                                                                                                            cGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAACATGGCGAAGCAAGCGCGTGACCAAATCGGTg                                     <  4:299147/149‑1 (MQ=255)
                                                                                                             ggTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAACATGGCGAAGCAAGCGCGTGACCAAATCGGTGc                                    >  5:190244/1‑149 (MQ=255)
                                                                                                              gTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGt                                                                                            <  3:154102/92‑1 (MQ=255)
                                                                                                              gTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGt                                                                                            >  4:154102/1‑92 (MQ=255)
                                                                                                               tCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAACATGGCGAAGCAAGCGCGTGACCAAATCGGTGCTg                                  <  2:327171/149‑1 (MQ=255)
                                                                                                                    gCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAACATGGCGAAGCAAGCGCGTGACCAAATCAGTGCTGAATCg                             >  1:306685/1‑149 (MQ=255)
                                                                                                                        tGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAACATGGCGAAGCAAGCGCGTGACCAAATCGGTGCTGAATCGCTGa                         >  7:249550/1‑149 (MQ=255)
                                                                                                                              cGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAACATGGCGAAGCAAGCGCGTGACCAAATCGGTGCTGAATCGCTGACGGTCg                   <  5:127532/149‑1 (MQ=255)
                                                                                                                                       taACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAACATGGCGAAGCAAGCGCGTGACCAAATCGGTGCTGAATCGCTGACGGTCGTTGCCGAcc          >  1:342032/1‑149 (MQ=255)
                                                                                                                                       taACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAACATGGCGAAGCAAGCGCGTGACCAAATCGGTGCTGAATCGCTGACGGTCGTTGCCGAcc          >  7:280516/1‑149 (MQ=255)
                                                                                                                                         aCGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAACATGGCGAAGCAAGCGCGTGACCAAATCGGTGCTGAATCGCTGACGGTCGTTGCCGACCgg        <  1:354015/149‑1 (MQ=255)
                                                                                                                                            tACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCCACATGGCGAAGCAAGCGCGTGACCAAATCGGTGCTGAATCGCTGACGGTCGTTGCCGACCGGGGc     >  1:326123/1‑149 (MQ=255)
                                                                                                                                              cAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAACATGGCGAAGCAAGCGCGTGACCAAATCGGTGCTGAATCGCTGACGGTCGTTGCCGACCGGGGCTa   <  6:190244/149‑1 (MQ=255)
                                                                                                                                               aaaCGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAACATGGCGAAGCAAGCGCGTGACCAAATCGGTGc                                    >  4:145516/1‑115 (MQ=255)
                                                                                                                                               aaaCGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAACATGGCGAAGCAAGCGCGTGACCAAATCGGTGCTGAATCGCTGACGGTCGTTGCCGACCGGGGCTAt  >  3:175775/1‑149 (MQ=255)
                                                                                                                                               aaaCGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAACATGGCGAAGCAAGCGCGTGACCAAATCGGTGCTGAATCGCTGACGGTCGTTGCCGACCGGGGCTAt  >  6:107551/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GCTGAAACAACAGATGCAGAAGCTCAAAGATATTGAAACGCAGCTACTTCAAACCCCAGACAAACAAATCTCTCTCACAGATCCCGATGCCCGCTCCATGTCAGTTGGCGGTCGGGGCAGTGGCACCGTTGGCTATAACGTACAAACGGCCGTTGACGATCAGCACCATCTGATCATTGCGCATGAAGTGACGAATGTCGGTCATGATCGCGGCCAGTTGAGCAACATGGCGAAGCAAGCGCGTGACCAAATCGGTGCTGAATCGCTGACGGTCGTTGCCGACCGGGGCTAT  >  NC_002947/2178376‑2178667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑