Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,236,973 T→G 8.7% intergenic (+68/+60) uvrB → / ← PP_1975 uvrABC excinuclease protein B/EmrB/QacA family drug resistance transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,236,9730TG 8.7% 124.7 / 3.4 46intergenic (+68/+60)uvrB/PP_1975uvrABC excinuclease protein B/EmrB/QacA family drug resistance transporter
Reads supporting (aligned to +/- strand):  ref base T (17/25);  new base G (2/2);  total (19/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

CCGCGACCTGGAGTTCGAAGCTGCGGCGCAGTTGCGTGACGAGATTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATGGGCTTCCCCAGCCTTCAACCCTTCCGGCAACTTGCGGGTCAGCAGCACCGCCACCATGCTCACCGCCAAC  >  NC_002947/2236827‑2237108
                                                                                                                                                  |                                                                                                                                       
ccGCGACCTGGAGTTCGAAGCTGCGGCGCAGTTGCGTGACGAGATTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTgc                                                                                                                                       >  4:239837/1‑149 (MQ=255)
ccGCGACCTGGAGTTCGAAGCTGCGGCGCAGTTGCGTGACGAGATTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTgc                                                                                                                                       <  1:267443/149‑1 (MQ=255)
  gcgACCTGGAGTTCGAAGCTGCGGCGCAGTTGCGTGACGAGATTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTgcgc                                                                                                                                     >  7:105282/1‑149 (MQ=255)
        tGGAGTTCGAAGCTGCGGCGCAGTTGCGTGACGAGATTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTTGGAGCGGGCGTGGCCGCGAAGAATTAAAAGCGGTGGCTGGCACCCGCTGCGCCCgtgt                                                                                                                               >  6:54104/1‑149 (MQ=255)
               cGAAGCTGCGGCGCAGTTGCGTGACGAGATTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCgg                                                                                                                         <  3:114048/148‑1 (MQ=255)
                     tGCGGCGCAGTTGCGTGACGAGATTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCGCAGCCGgtgcca                                                                                                                             <  2:242339/138‑4 (MQ=255)
                     tGCGGCGCAGTTGCGTGACGAGATTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCGCAGCCGgtgcca                                                                                                                             >  1:242339/1‑135 (MQ=255)
                         gcgcAGTTGCGTGACGAGATTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCt                                                                                                              >  7:62167/1‑149 (MQ=255)
                             aGTTGCGTGACGAGATTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCg                                                                                                                                   >  3:152842/1‑124 (MQ=255)
                             aGTTGCGTGACGAGATTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCg                                                                                                                                   <  4:152842/124‑1 (MQ=255)
                             aGTTGCGTGACGAGATTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCca                                                                                                          <  8:19514/149‑1 (MQ=255)
                                 gCGTGACGAGATTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAg                                                                                                       <  1:32474/148‑1 (MQ=255)
                                    tGACGAGATTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGAc                                                                                                   <  3:215953/149‑1 (MQ=255)
                                     gACGAGATTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGAcc                                                                                                  >  1:6449/1‑149 (MQ=255)
                                       cGAGATTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGc                                                                                                <  8:62167/149‑1 (MQ=255)
                                         agaTTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCat                                                                                              <  6:183317/149‑1 (MQ=255)
                                          gaTTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCatc                                                                                             <  5:313955/149‑1 (MQ=255)
                                          gaTTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCatc                                                                                             >  1:185296/1‑149 (MQ=255)
                                          gaTTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCGCAGCCGgtgc                                                                                                                               <  8:65830/115‑2 (MQ=255)
                                          gaTTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCGCAGCCGgtgc                                                                                                                               >  7:65830/1‑114 (MQ=255)
                                                gcAGCTGCGGGAGCGGTTGATCCGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCatctgatct                                                                                       <  6:109228/149‑1 (MQ=255)
                                                gcAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCatctgatct                                                                                       <  7:57699/149‑1 (MQ=255)
                                                  aGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTAAACGCGGTGGCTGGCACCCGCTGCGCCCGTGTTAGCGGGCCTTCCCCCACCCACAGGGCCCGCatctgaacttg                                                                                     >  4:66817/1‑147 (MQ=25)
                                                       cGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAAt                                                                                  <  5:54104/147‑1 (MQ=255)
                                                       cGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATa                                                                                 <  7:308886/148‑1 (MQ=255)
                                                       cGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATa                                                                                 <  2:107280/148‑1 (MQ=255)
                                                         ggAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGcc                                                                              <  3:239837/149‑1 (MQ=255)
                                                                ttGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATgg                                                                       <  2:185296/149‑1 (MQ=255)
                                                                      agcagcTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTcc                                                                                                            <  7:69186/106‑1 (MQ=255)
                                                                      agcagcTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTcc                                                                                                            >  8:69186/1‑106 (MQ=255)
                                                                          gcTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCcaca                                                                                                        <  7:107967/106‑1 (MQ=255)
                                                                          gcTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCcaca                                                                                                        >  8:107967/1‑106 (MQ=255)
                                                                              aaGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATGGGCTTCCCCAGCCt                                                          <  3:66817/148‑1 (MQ=255)
                                                                                       gcgGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATGGGCTTCCCCAGCCTTCAACCCTTc                                                <  8:105282/149‑1 (MQ=255)
                                                                                        cgGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATGGGCTTCCCCAGCCTTCAACCCTTcc                                               <  2:6449/149‑1 (MQ=255)
                                                                                                     aGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATGGGCTTCCCCAGCCTTCAACCCTTCCGGCAACTTGCggg                                  >  4:37586/1‑149 (MQ=255)
                                                                                                      gCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATGGGCTTCCCCAGCCTTCAACCCTTCCGGCAACTTGCGGGt                                 >  4:118316/1‑149 (MQ=255)
                                                                                                            gTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATGGGCTTCCCCAGCCTTCAACCCTTCCGGCAACTTGCGGGTcagcag                           >  8:193966/1‑149 (MQ=255)
                                                                                                               cccGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATGGGCTTCCCCAGTCTTCAACCCTTCCGGCAACTTGCGGGTCAGCAGCAc                        >  6:127714/1‑149 (MQ=255)
                                                                                                                ccGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATGGGCTTCCCCAGCCTTCAACCCTTCCGGCAACTTGCGGGTCAGCAGCAcc                       <  3:37586/149‑1 (MQ=255)
                                                                                                                         aTTCAACGCGGTGGCTGGCACCGGCTTCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATGGGCTTCCCCAGCCTTCAACCCTTCCGGCAACTTGCGGGTCAGCAGCACCGCCACCATg              <  3:118316/149‑1 (MQ=255)
                                                                                                                          ttCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATGGGCTTCCCCAGCCTTTAACCCTTCCGGCAACTTGCGGGTCAGCAGCACCGCCACCATGc             >  2:129649/1‑149 (MQ=255)
                                                                                                                          ttCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATGGGCTTCCCCAGCCTTCAAcc                                                    >  5:156739/1‑110 (MQ=255)
                                                                                                                          ttCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATGGGCTTCCCCAGCCTTCAAcc                                                    <  6:156739/110‑1 (MQ=255)
                                                                                                                           tCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATGGGCTTCCCCAGCCTTCAACCCTTCCGGCAACTTGCGGGTCAGCCGCACCGCCACCATGCt            >  5:280232/1‑149 (MQ=255)
                                                                                                                                      gctggcACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATGGGCTTCCCCAGTCTTCAACCCTTCCGGCAACTTGCGGGTCAGCAGCACCGCCACCATGCTCACCGCCAAc  <  5:127714/148‑1 (MQ=255)
                                                                                                                                                  tGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATGGGCTTCCCCAGCCTTCAACCCTTCCGGCAACTTGCgg                                   >  4:221401/1‑103 (MQ=255)
                                                                                                                                                  tGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATGGGCTTCCCCAGCCTTCAACCCTTCCGGCAACTTGCgg                                   <  3:221401/103‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                       
CCGCGACCTGGAGTTCGAAGCTGCGGCGCAGTTGCGTGACGAGATTGCGCAGCTGCGGGAGCGGTTGATCAGCAGCTGAAGCCGGGCGCGGGCCCTGTGGGAGCGGGCGTGCCCGCGAAGAATTCAACGCGGTGGCTGGCACCGGCTGCGCCGGTGTTCGCGGGCATGCCCGCTCCCACAGGGACCGCATCTGATCTGTAATAGCCTCAATGGGCTTCCCCAGCCTTCAACCCTTCCGGCAACTTGCGGGTCAGCAGCACCGCCACCATGCTCACCGCCAAC  >  NC_002947/2236827‑2237108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑