Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 15,850 C→G 9.6% intergenic (+100/‑739) gyrB → / → PP_5421 DNA gyrase subunit B/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294715,8500CG 9.6% 140.1 / 7.8 52intergenic (+100/‑739)gyrB/PP_5421DNA gyrase subunit B/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (21/26);  new base G (2/3);  total (23/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01

ACTTCATCGAAAGTAACGCGCTGTCGGTGTCGAACCTGGACTTCTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGAGGAAGTGATTTGATGGGGCGCTGGAAGTGCTGAGAGGGTCGGCGGGCGGATCT  >  NC_002947/15704‑15983
                                                                                                                                                  |                                                                                                                                      
aCTTCATCGAAAGTAACGCGCTGTCGGTGTCGAACCTGGACTTCTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTcgc                                                                                                                                      <  7:114594/149‑1 (MQ=255)
     aTCGAAAGTAACGCGCTGTCGGTGTCGAACCTGGACTTCTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGc                                                                                                                                  <  6:340064/148‑1 (MQ=255)
      tCGAAAGTAACGCGCTGTCGGTGTCGAACCTGGACTTCTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCGAAGGCGGGC‑tt                                                                                                                               >  3:7459/1‑149 (MQ=255)
          aaGTAACGCGCTGTCGGTGTCGAACCTGGACTTCTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCGAAGGCGGGC‑ttgatg                                                                                                                           <  4:7459/149‑5 (MQ=255)
          aaGTAACGCGCTGTCGGTGTCGAACCTGGACTTCTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCGAAGGCGGGC‑ttgatg                                                                                                                           <  7:158654/149‑5 (MQ=255)
             tAACGCGCTGTCGGTGTCGAACCTGGACTTCTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTg                                                                                                                        >  2:166873/1‑149 (MQ=255)
             tAACGCGCTGTCGGTGTCGAACCTGGACTTCTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTg                                                                                                                        >  3:118948/1‑149 (MQ=255)
              aaCGCGCTGTCGGTGTCGAACCTGGACTTCTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTg                                                                                                                        >  4:9625/1‑148 (MQ=255)
               aCGCGCTGTCGGTGTCGAACCTGGACTTCTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGcc                                                                                                                      <  5:53715/149‑1 (MQ=255)
                 gcgcTGTCGGTGTCGAACCTGGACTTCTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTg                                                                                                                    >  7:247148/1‑149 (MQ=255)
                               gAACCTGGACTTCTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCGAAGGCGGGC‑ttga                                                                                                                             >  5:330713/1‑124 (MQ=255)
                               gAACCTGGACTTCTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCGAAGGCGGGC‑ttga                                                                                                                             <  6:330713/126‑3 (MQ=255)
                               gAACCTGGACTTCTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCggg                                                                                                      <  2:201443/149‑1 (MQ=255)
                                    tGGACTTCTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCAGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTACTGCCTGGGCGcc                                                                                                 >  5:340140/1‑149 (MQ=255)
                                      gACTTCTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGccc                                                                                                <  8:318349/148‑1 (MQ=255)
                                         ttCTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTACTGCCGGGGCGCCCTACt                                                                                            >  2:325902/1‑149 (MQ=255)
                                           cTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACGATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCGCTACTc                                                                                           <  2:275031/148‑1 (MQ=255)
                                           cTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTc                                                                                           <  8:221057/148‑1 (MQ=255)
                                           cTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTACGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTc                                                                                           <  1:257524/148‑1 (MQ=255)
                                             gATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCTCCCTACTCCGc                                                                                        >  2:190347/1‑149 (MQ=255)
                                             gATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCg                                                                                         >  8:296982/1‑148 (MQ=255)
                                              aTCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCg                                                                                         <  1:166873/147‑1 (MQ=255)
                                               tCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAAGTACTGCCGGGGCGCCCTACTCCGCt                                                                                       >  4:301132/1‑148 (MQ=255)
                                                  ggTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCAtt                                                                                   <  7:296982/149‑1 (MQ=255)
                                                  ggTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCAtt                                                                                   <  4:118852/149‑1 (MQ=255)
                                                    tgtgtAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTg                                                                                  <  4:118948/148‑1 (MQ=255)
                                                           gTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCCATCATTGGAAAatga                                                                          >  7:123682/1‑149 (MQ=255)
                                                            gacacaTAAAAGCTGCACTCTTTCACAAATAGATGACCGGCCTGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAatgat                                                                         <  8:333426/147‑1 (MQ=255)
                                                                    aaaGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCTCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATgg                                                                 <  2:19336/149‑1 (MQ=255)
                                                                     aaGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATggg                                                                <  3:9625/149‑1 (MQ=255)
                                                                      aGCTTCAAGCTTTCACAAATTGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAATCCCGCCTTCGCGGGC‑TTTTTTTTGCCTTGAATTACTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATgggg                                                               <  6:340140/149‑1 (MQ=255)
                                                                              gCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGa                                                       <  8:247148/149‑1 (MQ=255)
                                                                                tttCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGAg                                                      >  6:280234/1‑148 (MQ=255)
                                                                                    acaAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGAGGAAGt                                                 >  2:181394/1‑149 (MQ=255)
                                                                                    aaaaaTAGATGCCCGGCCCGGCTTCAACCATGGCCCCTGGCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGAGGAAGt                                                 <  8:123682/147‑1 (MQ=255)
                                                                                      aaaTAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGa                                                       <  5:123170/141‑1 (MQ=255)
                                                                                      aaaTAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGa                                                       >  6:123170/1‑141 (MQ=255)
                                                                                      aaaTAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGAGGAAGTGa                                               >  4:164003/1‑149 (MQ=255)
                                                                                       aaTAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGAGGAAGTGAt                                              <  1:181394/149‑1 (MQ=255)
                                                                                        aTAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGAGGAAGTGAtt                                             <  4:110860/149‑1 (MQ=255)
                                                                                        aTAGATGACCGGCCGGACTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGTAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAatga                                                                          <  4:68004/120‑1 (MQ=255)
                                                                                        aTAGATGACCGGCCGGACTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAatga                                                                          >  3:68004/1‑120 (MQ=255)
                                                                                                            cAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGAGGAAGTGATTTGATGGGGCGCTGGAAGTg                          <  5:280234/148‑1 (MQ=255)
                                                                                                            cAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGAGGAAGTGATTTGATGGGGCGCTGGAAGTGc                         >  1:149343/1‑149 (MQ=255)
                                                                                                                 aTGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCTTTGGAAAATGATGCAGATGGGGGGATTTGAGGAAGTGATTTGATGGGGCGCTGGAAGTGCTgaga                    <  1:190347/149‑1 (MQ=255)
                                                                                                                                  ccATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGAGGAAGTGATTTGATGGGGCGCTGGAAGTGCTGAGAGGGTCGGCGGGCGGATc   >  7:330416/1‑149 (MQ=255)
                                                                                                                                   cATCAAGCCCGCCTTCGCGGGCTTTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGAGGAAGTGATTTGATGGGGCGCTGGAAGTGCTGAGAGGGTCGGCGGGCGGATc   >  7:37420/1‑149 (MQ=255)
                                                                                                                                   cATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGAGGAAGTGATTTGATGGGGCGCTGGAAGTg                          >  7:331779/1‑125 (MQ=255)
                                                                                                                                   cATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGAGGAAGTGATTTGATGGGGCGCTGGAAGTg                          <  8:331779/125‑1 (MQ=255)
                                                                                                                                   cATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGAGGAAGTGATTTGATGGGGCGCTGGAAGTGCTGAGAGGGTCGgcgggcgg      >  8:21387/1‑145 (MQ=255)
                                                                                                                                   cATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGAGGAAGTGATTTGATGGGGCGCTGGAAGTGCTGAGAGGGTCGgcgggcgg      <  7:21387/145‑1 (MQ=255)
                                                                                                                                   cATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGAGGAAGTGATTTGATGGGGCGCTGGAAGTGCTGAGAGGGTCGGCGGGCGGATCt  >  8:320548/1‑149 (MQ=255)
                                                                                                                                              ccTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGAGGAAGTGATTTGATGGGGCGCTAGAAGTGCTGAGAGGGTCggcgggc        <  4:276313/132‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                      
ACTTCATCGAAAGTAACGCGCTGTCGGTGTCGAACCTGGACTTCTGATCAGGTGTGTAGGTTCACAGAAAAGCTGCACGCTTTCACAAATAGATGACCGGCCGGGCTTCAACCATGGCCCCTGTCGGTCACCATCAAGCCCGCCTTCGCGGGC‑TTTTTTTTGCCTGGAATTCCTGCCGGGGCGCCCTACTCCGCTCATTGGAAAATGATGCAGATGGGGGGATTTGAGGAAGTGATTTGATGGGGCGCTGGAAGTGCTGAGAGGGTCGGCGGGCGGATCT  >  NC_002947/15704‑15983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑