BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,297,143	G→C	5.4%	D993E (GAC→GAG) ‡	sbcC ←	exonuclease subunit SbcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,297,143	0	G	C	5.4%	382.5 / 5.8	117	D993E (GAC→GAG) ‡	sbcC	exonuclease subunit SbcC
Reads supporting (aligned to +/- strand): ref base G (54/56); new base C (4/3); total (58/59)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

GTTCGATTTCTGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAGGCGATC  >  NC_002947/2296995‑2297289

gTTCGATTTCTGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCgtggtg                                                                                                                                                    >  5:123124/1‑149 (MQ=255)

 ttCGATTTCTGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGgtgt                                                                                                                                                   >  6:37803/1‑149 (MQ=255)

     aTTTCTGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCAc                                                                                                                                               >  4:141473/1‑149 (MQ=255)

       ttCTGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACgg                                                                                                                                             >  8:207617/1‑149 (MQ=255)

           gccgccAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGACACCACGGTcc                                                                                                                                          >  1:363264/1‑147 (MQ=255)

            ccgccAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATc                                                                                                                                        >  2:244786/1‑149 (MQ=255)

            ccgccAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGACACCACGGTc                                                                                                                                           <  5:226337/146‑1 (MQ=255)

             cgccAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCt                                                                                                                                       <  6:123124/149‑1 (MQ=255)

             cgccAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGACACCACGGTccaag                                                                                                                                       <  2:363264/149‑5 (MQ=255)

                cAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCtgtg                                                                                                                                    >  3:58320/1‑149 (MQ=255)

                      cTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGt                                                                                                                              >  3:86285/1‑149 (MQ=255)

                      cTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTCGACCGTGGTGTCAACGGTCATCTGTGCCGCGt                                                                                                                              >  2:101629/1‑149 (MQ=255)

                            ggCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGAtgct                                                                                                                        <  8:209711/149‑1 (MQ=255)

                             gCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGAtgctg                                                                                                                       <  5:367427/149‑1 (MQ=255)

                              ccTGCTGGGGGCGCTGGTCGTCGGCCTGTTGTTCACGCAATTCGGCGGATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCTGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCcgc                                                                                                                                <  7:275676/139‑1 (MQ=255)

                              ccTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGAtgctgc                                                                                                                      <  1:239890/149‑1 (MQ=255)

                              ccTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGAtgctgc                                                                                                                      <  3:200906/149‑1 (MQ=255)

                              ccTGCTCGCGGCGCTGGACGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTTCTCGTGGATTGCCAGGCGT‑TCGCGCAGTTCCGCCAAGGCATGCTCCAGGCCTTGGACCGTGGTCTCCACGGTCATCTGTGCcgc                                                                                                                                >  8:275676/1‑139 (MQ=255)

                                  ctgGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGgc                                                                                                                  >  3:33328/1‑149 (MQ=255)

                                  ctgGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGgc                                                                                                                  <  8:13179/149‑1 (MQ=255)

                                   tgGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGgcg                                                                                                                 <  4:300975/149‑1 (MQ=255)

                                     gcggcgCTGGTCGGCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGgcg                                                                                                                 <  5:367272/147‑1 (MQ=255)

                                       ggcgCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGt                                                                                                             >  5:107193/1‑149 (MQ=255)

                                       ggcgCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGt                                                                                                             <  4:129239/149‑1 (MQ=255)

                                          gcTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGt                                                                                                             <  3:236890/146‑1 (MQ=255)

                                          gcTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGt                                                                                                             >  4:236890/1‑146 (MQ=255)

                                                 gtcgGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTGCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCt                                                                                                   >  4:71839/1‑149 (MQ=255)

                                                   cgGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTgc                                                                                                 <  1:248827/149‑1 (MQ=255)

                                                      ccTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGGGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTgcagc                                                                                              <  3:312201/149‑1 (MQ=255)

                                                              gCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCTCGTGATGCTGCAGGCGCTGTTCGCGCTcc                                                                                                    <  1:337842/135‑1 (MQ=255)

                                                                aCGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGc                                                                                    <  1:168215/149‑1 (MQ=255)

                                                                aCGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGACTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGc                                                                                    >  5:160193/1‑149 (MQ=255)

                                                                 cGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGcc                                                                                   <  3:57656/149‑1 (MQ=255)

                                                                        cGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTGTCGCGCAGTTCGGCCAAGGATTGCTCCAGGGATTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTc                                                                             >  1:226714/1‑149 (MQ=255)

                                                                            gcATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAg                                                                                                                    <  5:275683/105‑1 (MQ=255)

                                                                            gcATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAg                                                                                                                    >  6:275683/1‑105 (MQ=255)

                                                                            gcATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATgg                                                                        <  3:71839/149‑1 (MQ=255)

                                                                                  ctgctCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTc                                                                                                                                           <  5:38213/76‑1 (MQ=255)

                                                                                  ctgctCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTc                                                                                                                                           >  6:38213/1‑76 (MQ=255)

                                                                                   tgctCGTCGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCATGGCATGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCACGCGATGTTCGCTCTCCTGCAGCAGGACGCGGCCCTGCTCGATGCCTTTTTc                                                                 >  4:248166/1‑149 (MQ=255)

                                                                                     ctCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCcg                                                               >  6:140968/1‑149 (MQ=255)

                                                                                        gTGGTTTGCCAGGCGT‑TCTCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCgcgc                                                            <  2:226714/149‑1 (MQ=255)

                                                                                        gTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACGGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCcgc                                                              <  6:40680/147‑1 (MQ=255)

                                                                                         tGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGcc                                                           >  4:24939/1‑149 (MQ=255)

                                                                                          ggTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCt                                                          >  6:105255/1‑149 (MQ=255)

                                                                                           gTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCt                                                                              <  5:134870/128‑1 (MQ=255)

                                                                                           gTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCt                                                                              >  6:134870/1‑128 (MQ=255)

                                                                                           gTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGC‑TTTTCCGCGCCTTg                                                        >  7:130799/1‑149 (MQ=255)

                                                                                           gTTGGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGGCCACGGTCATCTGGGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCtt                                                         <  4:96927/149‑1 (MQ=255)

                                                                                                ccAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGcc                                                                                   <  8:163921/118‑1 (MQ=255)

                                                                                                ccAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGACACCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCaaa                                                    >  1:143900/1‑149 (MQ=255)

                                                                                                 cAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAAc                                                   >  6:316731/1‑149 (MQ=255)

                                                                                                   ggCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCTTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGc                                                 <  7:200769/149‑1 (MQ=255)

                                                                                                    gCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGCGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCt                                                >  6:60887/1‑149 (MQ=255)

                                                                                                     cGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGACTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTTATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTg                                               <  6:160193/149‑1 (MQ=255)

                                                                                                          cgcgCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCCGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGgcg                                            >  7:92169/1‑148 (MQ=255)

                                                                                                             gcAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAAt                                        <  5:37803/149‑1 (MQ=255)

                                                                                                               aGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTc                                      <  8:253100/149‑1 (MQ=255)

                                                                                                                gTTCGGCCTAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACTCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCa                                     <  5:266391/149‑1 (MQ=255)

                                                                                                                gTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCCGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCa                                     <  7:207617/149‑1 (MQ=255)

                                                                                                                 ttCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCGGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAAc                                                   <  4:303748/134‑1 (MQ=255)

                                                                                                                 ttCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCGGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAAc                                                   >  3:303748/1‑134 (MQ=255)

                                                                                                                 ttCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCAt                                    <  5:316731/149‑1 (MQ=255)

                                                                                                                 ttCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCAt                                    >  7:295501/1‑149 (MQ=255)

                                                                                                                   cGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCAtt                                   <  4:33328/148‑1 (MQ=255)

                                                                                                                    ggCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTg                                                                                                  <  7:30404/84‑1 (MQ=255)

                                                                                                                    ggCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTg                                  <  1:244786/148‑1 (MQ=255)

                                                                                                                    ggCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAAATCATTGa                                 >  1:350569/1‑149 (MQ=255)

                                                                                                                      ccAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACt                               >  8:113062/1‑149 (MQ=255)

                                                                                                                         aGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTgc                            <  2:83584/149‑1 (MQ=255)

                                                                                                                          ggCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTgcg                           <  5:132323/149‑1 (MQ=255)

                                                                                                                          ggCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTgcg                           >  3:173231/1‑149 (MQ=255)

                                                                                                                               gCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGt                        <  7:113062/147‑1 (MQ=255)

                                                                                                                                 tCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATc                    >  4:13071/1‑149 (MQ=255)

                                                                                                                                 tCCAGGGCTTGGACCGTGGTGCCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATc                    >  8:164170/1‑149 (MQ=255)

                                                                                                                                   cAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTgcgc                          >  8:344581/1‑141 (MQ=255)

                                                                                                                                   cAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTgcgc                          <  7:344581/141‑1 (MQ=255)

                                                                                                                                   cAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCa                  >  5:303301/1‑149 (MQ=255)

                                                                                                                                   cAGCGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGAGATGCTGCAGGCGCAGTACGCGCTCCTGAAGAAGGACGAGGCCCTGCTCGATGGCTATTTCCGCGCCTTGCAAACGCTGGCGAAATTCAATGACATGCGAGTCATCCa                  >  8:231853/1‑149 (MQ=255)

                                                                                                                                     gggCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTgc                            >  3:145817/1‑137 (MQ=255)

                                                                                                                                     gggCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTgc                            <  4:145817/137‑1 (MQ=255)

                                                                                                                                      ggCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCtt                                                         >  8:322147/1‑107 (MQ=255)

                                                                                                                                      ggCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATg                <  6:90080/148‑1 (MQ=255)

                                                                                                                                      ggCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATg                >  5:90080/1‑148 (MQ=255)

                                                                                                                                         gatgACCGTGGACACCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATc                    <  1:100131/138‑1 (MQ=255)

                                                                                                                                         gatgACCGTGGACACCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATc                    >  2:100131/4‑141 (MQ=255)

                                                                                                                                            gACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATTGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCcagc          <  7:232239/148‑1 (MQ=255)

                                                                                                                                            gACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCcagc          <  8:295501/148‑1 (MQ=255)

                                                                                                                                             aCCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCcagcag        <  1:101629/149‑1 (MQ=255)

                                                                                                                                             aCCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCcagcag        <  4:58320/149‑1 (MQ=255)

                                                                                                                                              ccGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAgg       <  3:13071/149‑1 (MQ=255)

                                                                                                                                               cGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATc                    >  6:206914/1‑135 (MQ=255)

                                                                                                                                               cGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATc                    <  5:206914/135‑1 (MQ=255)

                                                                                                                                               cGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAGGc      >  6:491/1‑149 (MQ=255)

                                                                                                                                                 tggtgTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCcagc          <  8:50916/143‑1 (MQ=255)

                                                                                                                                                 tggtgTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAGGCg     >  8:164768/1‑148 (MQ=255)

                                                                                                                                                 tggtgTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAGGCGa    >  6:282804/1‑149 (MQ=255)

                                                                                                                                                  ggtgTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAGGCGAt   >  8:197202/1‑149 (MQ=255)

                                                                                                                                                   gtgtCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAGGCGAt   <  4:45629/148‑1 (MQ=255)

                                                                                                                                                     gtCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAGGCGATc  >  8:262683/1‑147 (MQ=255)

GTTCGATTTCTGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAGGCGATC  >  NC_002947/2296995‑2297289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`