BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,297,144	T→A	5.7%	D993V (GAC→GTC) ‡	sbcC ←	exonuclease subunit SbcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,297,144	0	T	A	5.7%	356.9 / 5.9	122	D993V (GAC→GTC) ‡	sbcC	exonuclease subunit SbcC
Reads supporting (aligned to +/- strand): ref base T (58/55); new base A (3/4); total (62/60)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 126 total aligned reads displayed.

TTCGATTTCTGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAGGCGATC  >  NC_002947/2296996‑2297289

ttCGATTTCTGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGgtgt                                                                                                                                                   >  6:37803/1‑149 (MQ=255)

ttCGATTTCTGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGgtgt                                                                                                                                                   >  7:253100/1‑149 (MQ=255)

      ttCTGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACgg                                                                                                                                             >  8:207617/1‑149 (MQ=255)

          gccgccAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGACACCACGGTcc                                                                                                                                          >  1:363264/1‑147 (MQ=255)

           ccgccAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATc                                                                                                                                        >  2:244786/1‑149 (MQ=255)

           ccgccAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGACACCACGGTc                                                                                                                                           <  5:226337/146‑1 (MQ=255)

            cgccAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCt                                                                                                                                       <  6:123124/149‑1 (MQ=255)

            cgccAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGACACCACGGTccaag                                                                                                                                       <  2:363264/149‑5 (MQ=255)

             gccAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCtg                                                                                                                                      >  7:115194/1‑149 (MQ=255)

              ccAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCtgt                                                                                                                                     >  4:57656/1‑149 (MQ=255)

               cAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCtgtg                                                                                                                                    >  3:58320/1‑149 (MQ=255)

                     cTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGt                                                                                                                              >  3:86285/1‑149 (MQ=255)

                       ggtggGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGa                                                                                                                            >  4:200906/1‑149 (MQ=255)

                           ggCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGAtgct                                                                                                                        <  8:209711/149‑1 (MQ=255)

                            gCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGAtgctg                                                                                                                       <  5:367427/149‑1 (MQ=255)

                             ccTGCTGGGGGCGCTGGTCGTCGGCCTGTTGTTCACGCAATTCGGCGGATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCTGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCcgc                                                                                                                                <  7:275676/139‑1 (MQ=255)

                             ccTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGAtgctgc                                                                                                                      <  3:141473/149‑1 (MQ=255)

                             ccTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGAtgctgc                                                                                                                      <  1:239890/149‑1 (MQ=255)

                             ccTGCTCGCGGCGCTGGACGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTTCTCGTGGATTGCCAGGCGT‑TCGCGCAGTTCCGCCAAGGCATGCTCCAGGCCTTGGACCGTGGTCTCCACGGTCATCTGTGCcgc                                                                                                                                >  8:275676/1‑139 (MQ=255)

                                  tgGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGgcg                                                                                                                 <  4:300975/149‑1 (MQ=255)

                                      ggcgCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGt                                                                                                             <  4:129239/149‑1 (MQ=255)

                                      ggcgCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGt                                                                                                             >  5:107193/1‑149 (MQ=255)

                                         gcTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGt                                                                                                             <  3:236890/146‑1 (MQ=255)

                                         gcTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGt                                                                                                             >  4:236890/1‑146 (MQ=255)

                                                gtcgGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTGCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCt                                                                                                   >  4:71839/1‑149 (MQ=255)

                                                  cgGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTgc                                                                                                 <  1:248827/149‑1 (MQ=255)

                                                     ccTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGGGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTgcagc                                                                                              <  3:312201/149‑1 (MQ=255)

                                                         ttgtGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAgg                                                                                           >  8:200769/1‑148 (MQ=255)

                                                             gCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCTCGTGATGCTGCAGGCGCTGTTCGCGCTcc                                                                                                    <  1:337842/135‑1 (MQ=255)

                                                             gCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCCCGTGATGCTGCAGGCGCTGTTCGCGCTcc                                                                                                    >  2:337842/1‑135 (MQ=255)

                                                               aCGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGc                                                                                    <  1:168215/149‑1 (MQ=255)

                                                                cGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGcc                                                                                   <  3:57656/149‑1 (MQ=255)

                                                                       cGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTGTCGCGCAGTTCGGCCAAGGATTGCTCCAGGGATTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTc                                                                             >  1:226714/1‑149 (MQ=255)

                                                                           gcATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAg                                                                                                                    >  6:275683/1‑105 (MQ=255)

                                                                           gcATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAg                                                                                                                    <  5:275683/105‑1 (MQ=255)

                                                                           gcATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATgg                                                                        >  3:45629/1‑149 (MQ=255)

                                                                                 ctgctCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTc                                                                                                                                           <  5:38213/76‑1 (MQ=255)

                                                                                 ctgctCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTc                                                                                                                                           >  6:38213/1‑76 (MQ=255)

                                                                                  tgctCGTCGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCATGGCATGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCACGCGATGTTCGCTCTCCTGCAGCAGGACGCGGCCCTGCTCGATGCCTTTTTc                                                                 >  4:248166/1‑149 (MQ=255)

                                                                                     tCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCcgc                                                              >  6:266391/1‑149 (MQ=255)

                                                                                       gTGGTTTGCCAGGCGT‑TCTCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCgcgc                                                            <  2:226714/149‑1 (MQ=255)

                                                                                       gTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCcgc                                                              >  5:40680/1‑147 (MQ=255)

                                                                                         ggTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCt                                                          >  6:105255/1‑149 (MQ=255)

                                                                                          gTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCt                                                                              >  6:134870/1‑128 (MQ=255)

                                                                                          gTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCt                                                                              <  5:134870/128‑1 (MQ=255)

                                                                                          gTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGC‑TTTTCCGCGCCTTg                                                        >  7:130799/1‑149 (MQ=255)

                                                                                          gTTGGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGGCCACGGTCATCTGGGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCtt                                                         <  4:96927/149‑1 (MQ=255)

                                                                                               ccAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGcc                                                                                   <  8:163921/118‑1 (MQ=255)

                                                                                               ccAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGACACCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCaaa                                                    >  1:143900/1‑149 (MQ=255)

                                                                                                cAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAAc                                                   >  6:316731/1‑149 (MQ=255)

                                                                                                 aGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACg                                                  >  6:132323/1‑149 (MQ=255)

                                                                                                  ggCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCTTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGc                                                 <  7:200769/149‑1 (MQ=255)

                                                                                                   gCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGCGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCt                                                >  6:60887/1‑149 (MQ=255)

                                                                                                    cGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGACTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTTATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTg                                               <  6:160193/149‑1 (MQ=255)

                                                                                                       gtCGCGCAGTTAGGCAATGTCTTGCTCCTGGTCTTTGACCTTGGTGACCACGGTCATCTGTGCCTCGTGATGCTGAAGGGGCTGTCCGCGGGCCTGAAGCTGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGgc                                             <  6:52689/148‑1 (MQ=255)

                                                                                                         cgcgCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCCGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGgcg                                            >  7:92169/1‑148 (MQ=255)

                                                                                                          gctcAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGgcgc                                           <  4:288163/145‑1 (MQ=255)

                                                                                                            gcAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAAt                                        <  5:37803/149‑1 (MQ=255)

                                                                                                              aGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTc                                      <  8:253100/149‑1 (MQ=255)

                                                                                                                ttCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCGGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAAc                                                   <  4:303748/134‑1 (MQ=255)

                                                                                                                ttCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCGGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAAc                                                   >  3:303748/1‑134 (MQ=255)

                                                                                                                ttCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCAt                                    >  7:295501/1‑149 (MQ=255)

                                                                                                                ttCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCAt                                    <  5:316731/149‑1 (MQ=255)

                                                                                                                  cGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCAtt                                   <  4:33328/148‑1 (MQ=255)

                                                                                                                   ggCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTg                                  <  1:244786/148‑1 (MQ=255)

                                                                                                                   ggCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAAATCATTGa                                 >  1:350569/1‑149 (MQ=255)

                                                                                                                     ccAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACt                               >  8:113062/1‑149 (MQ=255)

                                                                                                                         ggCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTgcg                           >  3:173231/1‑149 (MQ=255)

                                                                                                                         ggCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTgcg                           <  5:132323/149‑1 (MQ=255)

                                                                                                                          gCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTgcgc                          <  3:24939/149‑1 (MQ=255)

                                                                                                                                tCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATc                    >  3:89299/1‑149 (MQ=255)

                                                                                                                                tCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATc                    >  4:13071/1‑149 (MQ=255)

                                                                                                                                tCCAGGGCTTGGACCGTGGTGCCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATc                    >  8:164170/1‑149 (MQ=255)

                                                                                                                                  cAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAAc                                                   <  6:359272/116‑1 (MQ=255)

                                                                                                                                  cAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAAc                                                   >  5:359272/1‑116 (MQ=255)

                                                                                                                                  cAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTgcgc                          >  8:344581/1‑141 (MQ=255)

                                                                                                                                  cAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTgcgc                          <  7:344581/141‑1 (MQ=255)

                                                                                                                                  cAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCa                  >  5:303301/1‑149 (MQ=255)

                                                                                                                                  cAGCGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGAGATGCTGCAGGCGCAGTACGCGCTCCTGAAGAAGGACGAGGCCCTGCTCGATGGCTATTTCCGCGCCTTGCAAACGCTGGCGAAATTCAATGACATGCGAGTCATCCa                  >  8:231853/1‑149 (MQ=255)

                                                                                                                                   aGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCAt                 <  5:140968/149‑1 (MQ=255)

                                                                                                                                    gggCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTgc                            >  3:145817/1‑137 (MQ=255)

                                                                                                                                    gggCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTgc                            <  4:145817/137‑1 (MQ=255)

                                                                                                                                     ggCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCtt                                                         >  8:322147/1‑107 (MQ=255)

                                                                                                                                     ggCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATg                <  6:90080/148‑1 (MQ=255)

                                                                                                                                        ttGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGc              >  5:244966/1‑147 (MQ=255)

                                                                                                                                        gatgACCGTGGACACCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATc                    >  2:100131/4‑141 (MQ=255)

                                                                                                                                        gatgACCGTGGACACCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATc                    <  1:100131/138‑1 (MQ=255)

                                                                                                                                           gACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATTGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCcagc          <  7:232239/148‑1 (MQ=255)

                                                                                                                                           gACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCcagc          <  8:295501/148‑1 (MQ=255)

                                                                                                                                           gACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCcag           >  8:157280/1‑147 (MQ=255)

                                                                                                                                            aCCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCcagcag        <  1:101629/149‑1 (MQ=255)

                                                                                                                                            aCCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCcagcag        <  4:58320/149‑1 (MQ=255)

                                                                                                                                             ccGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAgg       <  3:13071/149‑1 (MQ=255)

                                                                                                                                              cGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATc                    >  6:206914/1‑135 (MQ=255)

                                                                                                                                              cGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATc                    <  5:206914/135‑1 (MQ=255)

                                                                                                                                              cGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAGGc      >  6:491/1‑149 (MQ=255)

                                                                                                                                                tggtgTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCcagc          >  7:50916/1‑143 (MQ=255)

                                                                                                                                                tggtgTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCcagc          <  8:50916/143‑1 (MQ=255)

                                                                                                                                                tggtgTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAGGCg     >  8:164768/1‑148 (MQ=255)

                                                                                                                                                    gtCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAGGCGATc  >  8:262683/1‑147 (MQ=255)

TTCGATTTCTGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGT‑TCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAGGCGATC  >  NC_002947/2296996‑2297289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`