BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,300,401	T→G	7.7%	E319A (GAG→GCG)	sbcD ←	exonuclease subunit SbcD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,300,401	0	T	G	7.7%	357.7 / 17.7	117	E319A (GAG→GCG)	sbcD	exonuclease subunit SbcD
Reads supporting (aligned to +/- strand): ref base T (59/49); new base G (5/4); total (64/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01

Only 100 of 117 total aligned reads displayed.

CGACAAAGGCCAGGTCATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGC  >  NC_002947/2300255‑2300548

cGACAAAGGCCAGGTCATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTc                                                                                                                                                    <  2:341414/148‑1 (MQ=255)

   cAAAGGCCAGGTCATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTccagc                                                                                                                                                <  2:298157/149‑1 (MQ=255)

   cAAAGGCCAGGTCATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGAGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTccagc                                                                                                                                                >  8:14667/1‑149 (MQ=255)

    aaaGGCCAGGTCATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTccagc                                                                                                                                                >  7:40200/1‑148 (MQ=255)

        gCCAGGTCATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAggg                                                                                                                                           >  3:124315/1‑149 (MQ=255)

               cATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCgg                                                                                                                                    >  4:213073/1‑149 (MQ=255)

                aTCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCCAACCGCCAGCCCTGGCTGGAGGt                                                                                                                                   >  5:368696/1‑149 (MQ=255)

                   gTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTgg                                                                                                                                >  8:262916/1‑149 (MQ=255)

                   gTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTgg                                                                                                                                <  4:124315/149‑1 (MQ=255)

                    tCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTggg                                                                                                                               <  3:205172/149‑1 (MQ=255)

                    tCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTggg                                                                                                                               <  3:213073/149‑1 (MQ=255)

                    tCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTggg                                                                                                                               <  5:224166/149‑1 (MQ=255)

                    tCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTggg                                                                                                                               <  8:245802/149‑1 (MQ=255)

                    tCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTggg                                                                                                                               <  8:34987/149‑1 (MQ=255)

                          gCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTc                                                                                                                         <  7:262916/149‑1 (MQ=255)

                           cGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCCAACCGCCAGCCCTGGCTGGAGGTGCGGGTGttgc                                                                                                                        <  6:368696/149‑3 (MQ=38)

                               gttgCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAg                                                                                                                      >  3:282317/1‑147 (MQ=255)

                                   cggccggcGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGtt                                                                                                                                  >  8:139392/1‑131 (MQ=255)

                                   cggccggcGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGtt                                                                                                                                  <  7:139392/131‑1 (MQ=255)

                                      ccggcGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGAc                                                                                                             >  7:61923/1‑149 (MQ=255)

                                       cggcGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCCGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGAc                                                                                                             >  8:60623/1‑148 (MQ=255)

                                          cGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACg                                                                                                         <  4:282317/149‑1 (MQ=255)

                                                         tACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGt                                                                                          >  5:334496/1‑149 (MQ=255)

                                                          aCGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCCGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGtt                                                                                         >  7:138454/1‑149 (MQ=255)

                                                            gAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGc                                                                                                                                          >  6:95018/1‑98 (MQ=255)

                                                            gAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGc                                                                                                                                          <  5:95018/98‑1 (MQ=255)

                                                            gAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGGCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTtgc                                                                                       >  3:196643/1‑149 (MQ=255)

                                                            gAATCAGCCGTACAGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTtgc                                                                                       >  5:275288/1‑149 (MQ=255)

                                                               tCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTtgctg                                                                                     >  2:82013/1‑148 (MQ=255)

                                                               tCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTtgctg                                                                                     >  4:133655/1‑148 (MQ=255)

                                                                  gCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCaa                                                                                  >  5:204581/1‑148 (MQ=255)

                                                                      tACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTc                                                                                                                         <  3:177397/105‑1 (MQ=255)

                                                                      tACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTc                                                                                                                         >  4:177397/1‑105 (MQ=255)

                                                                       aCCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAACTc                                                                            >  6:64137/1‑149 (MQ=255)

                                                                              tgccttCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGc                                                                     >  5:350349/1‑149 (MQ=255)

                                                                               gccttCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCg                                                                    >  8:111506/1‑149 (MQ=255)

                                                                                 cttCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGccc                                                                        <  2:297108/143‑1 (MQ=255)

                                                                                 cttCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGccc                                                                        >  1:297108/1‑143 (MQ=255)

                                                                                   tCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGc                                                                >  3:223022/1‑149 (MQ=255)

                                                                                      aaggcgGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCgg                                                             <  2:37585/146‑1 (MQ=255)

                                                                                       gcgcGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCggg                                                            >  6:281020/1‑149 (MQ=255)

                                                                                        cgcgGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGc                                                           >  5:9688/1‑149 (MQ=255)

                                                                                         gcgGTTTCGAGCTGCTCGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCGGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGGCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGcc                                                          <  3:42611/149‑1 (MQ=255)

                                                                                           ggTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCa                                                        >  6:74275/1‑149 (MQ=255)

                                                                                             tttCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGACCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCAcc                                                      <  8:61923/149‑1 (MQ=255)

                                                                                              ttCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGc                                                           >  1:216181/1‑143 (MQ=255)

                                                                                                cGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGcc                                                               <  5:238734/137‑1 (MQ=255)

                                                                                                cGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGcc                                                               >  6:238734/1‑137 (MQ=255)

                                                                                                  aTCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTg                                                 >  5:324859/1‑149 (MQ=255)

                                                                                                      gctgGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAg                                              >  6:137970/1‑148 (MQ=255)

                                                                                                      gctgGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAg                                              <  5:137970/148‑1 (MQ=255)

                                                                                                       ctgGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAgg                                             <  7:14667/148‑1 (MQ=255)

                                                                                                         ggCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCg                                                                    >  1:231027/1‑123 (MQ=255)

                                                                                                         ggCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCg                                                                    <  2:231027/123‑1 (MQ=255)

                                                                                                            gcAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCgg                                                                                               <  8:61764/93‑1 (MQ=255)

                                                                                                            gcAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCgg                                                                                               >  7:61764/1‑93 (MQ=255)

                                                                                                            gcAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCgg                                                             >  4:316081/1‑127 (MQ=255)

                                                                                                            gcAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCgg                                                             <  3:316081/127‑1 (MQ=255)

                                                                                                               gatCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGgcg                                    >  5:172165/3‑149 (MQ=255)

                                                                                                                 tCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGcgcg                                  >  6:74494/1‑149 (MQ=255)

                                                                                                                   gggTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTg                                <  7:123597/149‑1 (MQ=255)

                                                                                                                    ggTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTgg                               >  1:95732/1‑149 (MQ=255)

                                                                                                                     gTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGgc                                     >  3:344914/1‑142 (MQ=255)

                                                                                                                      ttGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGcgcg                                  >  3:3825/1‑144 (MQ=255)

                                                                                                                      ttGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGcgcg                                  <  4:3825/144‑1 (MQ=255)

                                                                                                                         cGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACgg                          <  2:311736/149‑1 (MQ=255)

                                                                                                                         cGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAACTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACgg                          <  5:64137/149‑1 (MQ=255)

                                                                                                                           gCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACgggg                        >  4:37252/1‑149 (MQ=255)

                                                                                                                            cTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGc                       >  7:340526/1‑149 (MQ=255)

                                                                                                                              cgtcgAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCAc                                                                                                          >  1:140880/1‑64 (MQ=255)

                                                                                                                              cgtcgAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCAc                                                                                                          <  2:140880/64‑1 (MQ=255)

                                                                                                                               gtcgAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTg                    <  2:16279/149‑1 (MQ=255)

                                                                                                                                tcgAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGcgcg                                  >  6:127010/1‑134 (MQ=255)

                                                                                                                                tcgAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTgg                   <  4:223022/149‑1 (MQ=255)

                                                                                                                                tcgAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTAGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTgg                   >  6:173333/1‑149 (MQ=255)

                                                                                                                                  gAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGATGCAGGGCCACGCCCCGTACCACGCGCCGTGGcc                 <  1:82013/149‑2 (MQ=255)

                                                                                                                                   aGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTc                <  4:190083/149‑1 (MQ=255)

                                                                                                                                     cAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGa              >  1:208008/1‑149 (MQ=255)

                                                                                                                                     cAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGa              >  2:265783/1‑149 (MQ=255)

                                                                                                                                      aaCACCCGCACCTCCAGCCAGGGCGGGCGGTTGGGGGCTTCAAGCAGGTCGACCACGGGCAGGGCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGAc             <  7:60623/149‑1 (MQ=255)

                                                                                                                                       acacCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACg            >  7:272178/1‑149 (MQ=255)

                                                                                                                                        cacCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGc           >  1:124380/1‑149 (MQ=255)

                                                                                                                                          cccGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTg         <  8:272178/149‑1 (MQ=255)

                                                                                                                                          cccGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTg         <  3:133655/149‑1 (MQ=255)

                                                                                                                                           cccaaCCGCCAGCCCTGGCTGGAGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTg                                <  4:238602/121‑1 (MQ=255)

                                                                                                                                           cccaaCCGCCAGCCCTGGCTGGAGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTg                                >  3:238602/5‑125 (MQ=255)

                                                                                                                                           cccaaCCGCCAGCCCTGGCTGGAGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGt                     >  4:79889/5‑136 (MQ=255)

                                                                                                                                           cccaaCCGCCAGCCCTGGCTGGAGGTGCGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGa        >  2:82406/5‑149 (MQ=255)

                                                                                                                                           ccGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGcc                                                               >  1:37231/1‑94 (MQ=255)

                                                                                                                                           ccGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTgg                   <  4:94493/138‑1 (MQ=255)

                                                                                                                                           ccGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTgg                   >  3:94493/1‑138 (MQ=255)

                                                                                                                                           ccGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCt                 >  7:92229/1‑140 (MQ=255)

                                                                                                                                           ccGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGa        >  5:365903/1‑149 (MQ=255)

                                                                                                                                            ccaaCCGCCAGCCCTGGCTGGAGGTGCGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGAc       >  4:228032/4‑149 (MQ=255)

                                                                                                                                            cGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGAc       >  3:211304/1‑149 (MQ=255)

                                                                                                                                            cGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGAc       <  8:340526/149‑1 (MQ=255)

                                                                                                                                                 cTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTgg                   <  3:204016/132‑1 (MQ=255)

                                                                                                                                                 cTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTgg                   >  4:204016/1‑132 (MQ=255)

                                                                                                                                                 cTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGc  >  2:10404/1‑149 (MQ=255)

                                                                                                                                                 cTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGc  <  5:173333/149‑1 (MQ=255)

CGACAAAGGCCAGGTCATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGC  >  NC_002947/2300255‑2300548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`