BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,300,409	G→T	8.2%	P316P (CCC→CCA)	sbcD ←	exonuclease subunit SbcD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,300,409	0	G	T	8.2%	349.8 / 16.3	110	P316P (CCC→CCA)	sbcD	exonuclease subunit SbcD
Reads supporting (aligned to +/- strand): ref base G (55/46); new base T (5/4); total (60/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01

Only 100 of 118 total aligned reads displayed.

GCCAGGTCATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCGTTACC  >  NC_002947/2300263‑2300555

gCCAGGTCATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAggg                                                                                                                                                  >  3:124315/1‑149 (MQ=255)

       cATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCgg                                                                                                                                           >  4:213073/1‑149 (MQ=255)

           gTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTgg                                                                                                                                       >  8:262916/1‑149 (MQ=255)

           gTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTgg                                                                                                                                       <  4:124315/149‑1 (MQ=255)

           gTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCGCCAGCCCTGGCTGGAGGtgc                                                                                                                                        <  3:224874/148‑3 (MQ=255)

            tCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTggg                                                                                                                                      <  5:224166/149‑1 (MQ=255)

            tCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTggg                                                                                                                                      <  8:245802/149‑1 (MQ=255)

            tCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTggg                                                                                                                                      <  3:205172/149‑1 (MQ=255)

            tCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTggg                                                                                                                                      <  8:34987/149‑1 (MQ=255)

                  gCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTc                                                                                                                                <  7:262916/149‑1 (MQ=255)

                       gttgCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTg                                                                                                                                        <  8:3130/136‑1 (MQ=255)

                       gttgCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAg                                                                                                                             >  3:282317/1‑147 (MQ=255)

                           cggccggcGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGtt                                                                                                                                         >  8:139392/1‑131 (MQ=255)

                           cggccggcGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGtt                                                                                                                                         <  7:139392/131‑1 (MQ=255)

                              ccggcGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGAc                                                                                                                    >  7:61923/1‑149 (MQ=255)

                               cggcGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCCGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGAc                                                                                                                    >  8:60623/1‑148 (MQ=255)

                                  cGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACg                                                                                                                <  4:282317/149‑1 (MQ=255)

                                                 tACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGt                                                                                                 >  5:334496/1‑149 (MQ=255)

                                                  aCGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCCGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGtt                                                                                                >  7:138454/1‑149 (MQ=255)

                                                    gAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGc                                                                                                                                                 <  5:95018/98‑1 (MQ=255)

                                                    gAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGc                                                                                                                                                 >  6:95018/1‑98 (MQ=255)

                                                    gAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGGCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTtgc                                                                                              >  3:196643/1‑149 (MQ=255)

                                                    gAATCAGCCGTACAGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTtgc                                                                                              >  5:275288/1‑149 (MQ=255)

                                                       tCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTtgctg                                                                                            >  4:133655/1‑148 (MQ=255)

                                                       tCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTtgctg                                                                                            >  2:82013/1‑148 (MQ=255)

                                                          gCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCaa                                                                                         >  5:204581/1‑148 (MQ=255)

                                                              tACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTc                                                                                                                                <  3:177397/105‑1 (MQ=255)

                                                               aCCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAACTc                                                                                   >  6:64137/1‑149 (MQ=255)

                                                                      tgccttCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGc                                                                            >  5:350349/1‑149 (MQ=255)

                                                                       gccttCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGTGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAg                                                                             <  6:275288/147‑1 (MQ=255)

                                                                       gccttCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCg                                                                           >  8:111506/1‑149 (MQ=255)

                                                                         cttCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGccc                                                                               >  1:297108/1‑143 (MQ=255)

                                                                         cttCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGccc                                                                               <  2:297108/143‑1 (MQ=255)

                                                                           tCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGc                                                                       >  3:223022/1‑149 (MQ=255)

                                                                              aaggcgGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCgg                                                                    <  2:37585/146‑1 (MQ=255)

                                                                               gcgcGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCggg                                                                   >  6:281020/1‑149 (MQ=255)

                                                                                cgcgGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGc                                                                  >  5:9688/1‑149 (MQ=255)

                                                                                 gcgGTTTCGAGCTGCTCGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCGGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGGCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGcc                                                                 <  3:42611/149‑1 (MQ=255)

                                                                                   ggTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCa                                                               >  6:74275/1‑149 (MQ=255)

                                                                                     tttCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGACCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCAcc                                                             <  8:61923/149‑1 (MQ=255)

                                                                                      ttCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGc                                                                  <  2:216181/143‑1 (MQ=255)

                                                                                      ttCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGc                                                                  >  1:216181/1‑143 (MQ=255)

                                                                                        cGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGcc                                                                      >  6:238734/1‑137 (MQ=255)

                                                                                          aTCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTg                                                        >  5:324859/1‑149 (MQ=255)

                                                                                              gctgGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAg                                                     <  5:137970/148‑1 (MQ=255)

                                                                                              gctgGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAg                                                     >  6:137970/1‑148 (MQ=255)

                                                                                               ctgGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAgg                                                    <  7:14667/148‑1 (MQ=255)

                                                                                                 ggCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCg                                                                           <  2:231027/123‑1 (MQ=255)

                                                                                                 ggCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCg                                                                           >  1:231027/1‑123 (MQ=255)

                                                                                                    gcAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCgg                                                                                                      >  7:61764/1‑93 (MQ=255)

                                                                                                    gcAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCgg                                                                                                      <  8:61764/93‑1 (MQ=255)

                                                                                                    gcAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCgg                                                                    >  4:316081/1‑127 (MQ=255)

                                                                                                    gcAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCgg                                                                    <  3:316081/127‑1 (MQ=255)

                                                                                                       gatCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGgcg                                           >  5:172165/3‑149 (MQ=255)

                                                                                                         tCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGcgcg                                         >  6:74494/1‑149 (MQ=255)

                                                                                                           gggTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTg                                       <  7:123597/149‑1 (MQ=255)

                                                                                                            ggTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTgg                                      >  1:95732/1‑149 (MQ=255)

                                                                                                             gTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGgc                                            <  4:344914/142‑1 (MQ=255)

                                                                                                             gTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGgc                                            >  3:344914/1‑142 (MQ=255)

                                                                                                              ttGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGcgcg                                         <  4:3825/144‑1 (MQ=255)

                                                                                                                 cGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACgg                                 <  2:311736/149‑1 (MQ=255)

                                                                                                                 cGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAACTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACgg                                 <  5:64137/149‑1 (MQ=255)

                                                                                                                   gCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACgggg                               >  4:37252/1‑149 (MQ=255)

                                                                                                                    cTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGc                              >  7:340526/1‑149 (MQ=255)

                                                                                                                    cTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGc                              <  5:74494/149‑1 (MQ=255)

                                                                                                                      cgtcgAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCAc                                                                                                                 <  2:140880/64‑1 (MQ=255)

                                                                                                                      cgtcgAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCAc                                                                                                                 >  1:140880/1‑64 (MQ=255)

                                                                                                                        tcgAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTgg                          <  4:223022/149‑1 (MQ=255)

                                                                                                                        tcgAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTAGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTgg                          >  6:173333/1‑149 (MQ=255)

                                                                                                                          gAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGATGCAGGGCCACGCCCCGTACCACGCGCCGTGGcc                        <  1:82013/149‑2 (MQ=255)

                                                                                                                           aGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTc                       <  4:190083/149‑1 (MQ=255)

                                                                                                                             cAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGa                     >  1:208008/1‑149 (MQ=255)

                                                                                                                             cAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGa                     >  2:265783/1‑149 (MQ=255)

                                                                                                                              aaCACCCGCACCTCCAGCCAGGGCGGGCGGTTGGGGGCTTCAAGCAGGTCGACCACGGGCAGGGCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGAc                    <  7:60623/149‑1 (MQ=255)

                                                                                                                               acacCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACg                   >  7:272178/1‑149 (MQ=255)

                                                                                                                                cacCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGc                  >  1:124380/1‑149 (MQ=255)

                                                                                                                                  cccGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTg                <  8:272178/149‑1 (MQ=255)

                                                                                                                                  cccGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTg                <  3:133655/149‑1 (MQ=255)

                                                                                                                                   cccaaCCGCCAGCCCTGGCTGGAGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTg                                       >  3:238602/5‑125 (MQ=255)

                                                                                                                                   cccaaCCGCCAGCCCTGGCTGGAGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGt                            <  3:79889/132‑1 (MQ=255)

                                                                                                                                   cccaaCCGCCAGCCCTGGCTGGAGGTGCGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGa               >  2:82406/5‑149 (MQ=255)

                                                                                                                                   ccGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGcc                                                                      >  1:37231/1‑94 (MQ=255)

                                                                                                                                   ccGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTgg                          <  4:94493/138‑1 (MQ=255)

                                                                                                                                   ccGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTgg                          >  3:94493/1‑138 (MQ=255)

                                                                                                                                   ccGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCt                        >  7:92229/1‑140 (MQ=255)

                                                                                                                                   ccGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGa               >  5:365903/1‑149 (MQ=255)

                                                                                                                                    ccaaCCGCCAGCCCTGGCTGGAGGTGCGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGAc              >  4:228032/4‑149 (MQ=255)

                                                                                                                                    cGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGAc              >  3:211304/1‑149 (MQ=255)

                                                                                                                                       aCCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAg           <  5:281020/149‑1 (MQ=255)

                                                                                                                                         cgccagccctGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCCCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGc         >  8:78078/3‑149 (MQ=255)

                                                                                                                                         cgccagccctGGCTGGAGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGAc                    >  1:354265/3‑138 (MQ=255)

                                                                                                                                         cTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTgg                          >  4:204016/1‑132 (MQ=255)

                                                                                                                                         cTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTgg                          <  3:204016/132‑1 (MQ=255)

                                                                                                                                         cTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGc         <  5:173333/149‑1 (MQ=255)

                                                                                                                                         cTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGc         >  2:10404/1‑149 (MQ=255)

                                                                                                                                           ccagccagGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCg       >  5:69060/1‑149 (MQ=255)

                                                                                                                                            cagccagGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGcc        <  6:204581/147‑1 (MQ=255)

                                                                                                                                            cagccagGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCGt      >  4:220849/1‑149 (MQ=255)

                                                                                                                                              gccagGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCGTTa    >  3:20219/1‑149 (MQ=255)

                                                                                                                                                cagGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCGTTAcc  <  6:9688/149‑1 (MQ=255)

GCCAGGTCATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGGTTGGGGTCTTCAAGCAGGTCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCGTTACC  >  NC_002947/2300263‑2300555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`