BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,312,842	C→G	6.9%	R5T (AGG→ACG) ‡	PP_2034 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,312,842	0	C	G	6.9%	301.1 / 8.5	98	R5T (AGG→ACG) ‡	PP_2034	membrane protein
Reads supporting (aligned to +/- strand): ref base C (46/44); new base G (4/3); total (51/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 103 total aligned reads displayed.

CGCCCAGCAGGCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGATGTTGGCCG  >  NC_002947/2312699‑2312988

cGCCCAGCAGGCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTgg                                                                                                                                                 <  6:238307/147‑1 (MQ=255)

 gCCCAGCGGGCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGgcg                                                                                                                                               >  1:38695/1‑148 (MQ=255)

 gCCCAGCAGGCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGgcg                                                                                                                                               >  2:5319/1‑148 (MQ=255)

 gCCCAGCAGGCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGgcg                                                                                                                                               >  8:194185/1‑148 (MQ=255)

   ccAGCAGGCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACCCCTTGCGCCCATGCCGCAc                                                                                                                                            >  2:2321/1‑149 (MQ=255)

   ccAGCAGGCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCAc                                                                                                                                            >  2:153734/1‑149 (MQ=255)

   ccAGCAGGCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCAc                                                                                                                                            >  2:130184/1‑149 (MQ=255)

   ccAGCAGGCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGGGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCAc                                                                                                                                            >  3:116641/1‑149 (MQ=255)

      gcagGCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGGGCGCCCCTGGCGCACGGc                                                                                                                                         <  5:372533/149‑1 (MQ=255)

       cagGCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGAGGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCAGGGGCGCACgctg                                                                                                                                        <  8:265303/149‑4 (MQ=255)

          gCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAgg                                                                                                                                     >  7:189677/1‑149 (MQ=255)

          gCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAgg                                                                                                                                     >  7:308462/1‑149 (MQ=255)

          gCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAgg                                                                                                                                     >  4:117098/1‑149 (MQ=255)

           ccGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGCGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTCCGCCCCTGGCGCACGGCAAggt                                                                                                                                    >  3:35978/1‑149 (MQ=255)

           ccGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGAAGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGTCGCACGGCAAggt                                                                                                                                    >  1:85863/1‑149 (MQ=255)

             gggCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAggtgg                                                                                                                                  <  2:38695/149‑1 (MQ=255)

               gcagcaCGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCAGGGGCGCACgctg                                                                                                                                        >  2:352908/1‑138 (MQ=255)

               gcagcaCGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCAGGGGCGCACgctg                                                                                                                                        <  1:352908/141‑4 (MQ=255)

                cagcaCGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGtggtgg                                                                                                                               <  1:5319/149‑1 (MQ=255)

                    aCGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAg                                                                                                                           >  5:215922/1‑149 (MQ=255)

                        gCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCTGCATTTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCg                                                                                                                       <  6:125027/149‑1 (MQ=255)

                          aGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCt                                                                                                                     >  8:294284/1‑149 (MQ=255)

                            tAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCt                                                                                                                     >  2:123830/1‑147 (MQ=255)

                               cgcCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCg                                                                                                                >  6:144081/1‑149 (MQ=255)

                               cgcCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCg                                                                                                                >  8:177139/1‑149 (MQ=255)

                                gcCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCgg                                                                                                               <  7:194185/149‑1 (MQ=255)

                                   gggATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAg                                                                                                            <  7:177139/149‑1 (MQ=255)

                                        cAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGcgcc                                                                                                       >  4:288976/1‑149 (MQ=255)

                                         aGCTTGATTTGCACATGCCTGACGAGTTGCACGGCAGCAATTTGGGTCAGCATCTTGGGGCCAGCGTGGTGCATGTCGATACCTGTTGTACTGCGTGCGCCGCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGTcgcc                                                                                                       <  8:154402/148‑1 (MQ=255)

                                         aGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGcgcc                                                                                                       <  7:7311/148‑1 (MQ=255)

                                          gCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGcgccgc                                                                                                     <  7:294284/149‑1 (MQ=255)

                                            atgatgTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACgg                                                                                                                                          <  2:262172/110‑1 (MQ=255)

                                            atgatgTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAACAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACgg                                                                                                                                          >  1:262172/1‑110 (MQ=255)

                                             tgatgTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCgcgc                                                                                                   >  6:10850/1‑148 (MQ=255)

                                                   gCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCgg                                                                                            >  5:232998/1‑149 (MQ=255)

                                                   gCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCgg                                                                                            >  4:245270/1‑149 (MQ=255)

                                                    cacaTGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCggg                                                                                           <  2:215880/149‑1 (MQ=255)

                                                       aTGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAg                                                                                        <  3:169791/149‑1 (MQ=255)

                                                           cTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGa                                                                                    <  3:117098/149‑1 (MQ=255)

                                                           cTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGa                                                                                    <  4:332238/149‑1 (MQ=255)

                                                              aCGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCa                                                                                         <  7:24702/141‑1 (MQ=255)

                                                              aCGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCa                                                                                         >  8:24702/1‑141 (MQ=255)

                                                              aCGAAGTGCACGGCAGCAATTTGGGGCAGCAGCTTGGGGCCAGCGGGGGGCATGTCGATACCTGTTCAACAGCGGGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGAcc                                                                                  <  1:130184/148‑1 (MQ=255)

                                                                gAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGtt                                                                               <  3:245270/149‑1 (MQ=255)

                                                                   gTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAg                                                                                                                  >  4:61837/1‑111 (MQ=255)

                                                                   gTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAg                                                                                                                  <  3:61837/111‑1 (MQ=255)

                                                                   gTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGCGCAGGCGACCGTTCAg                                                                            <  6:215922/149‑1 (MQ=255)

                                                                      aaCGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGCGCATGTCGATACCTGTTGAACAGCGTGCGCAACTGGCACACGGAAAGGTTGAGGAAAGCTCGCTTATCCGCAGCCGCCGCGCAGACCGGGCAGGCGACCGGTCTGGa                                                                          >  4:263393/2‑148 (MQ=255)

                                                                          gcagcaATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACg                                                                     >  3:50725/1‑149 (MQ=255)

                                                                            agcaATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCggg                                                                                           >  2:269173/1‑125 (MQ=255)

                                                                            agcaATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCggg                                                                                           <  1:269173/125‑1 (MQ=255)

                                                                               aaTTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGc                                                                <  1:153734/149‑1 (MQ=255)

                                                                               aaTTTGGGGCAGCAGCTTGGGGCCAGGGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGc                                                                <  3:20908/149‑1 (MQ=255)

                                                                                  ttGGGTCTGCAGCTTGGGGCCTGCGTGGGGCATGTCGATTCCTGTTGAACAGCGTGCGCCCCTGGCGCACGGGAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCca                                                              <  4:35978/148‑1 (MQ=255)

                                                                                     ggTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCcagca                                                           <  8:189677/148‑1 (MQ=255)

                                                                                          gcagcTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCaa                                                     >  4:205296/1‑149 (MQ=255)

                                                                                           cagcTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAAt                                                    >  8:289628/1‑149 (MQ=255)

                                                                                           cagcTTGGGGCCAGCGTGGGGAATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAAt                                                    <  8:141966/149‑1 (MQ=255)

                                                                                              cTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGAc                                                 <  4:50725/149‑1 (MQ=255)

                                                                                               ttGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGAc                                                 >  2:220563/1‑148 (MQ=255)

                                                                                                 ggggCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGTGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAg                                              >  8:286559/1‑149 (MQ=255)

                                                                                                  ggcccAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAg                                              <  8:308462/145‑1 (MQ=255)

                                                                                                    gCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGcc                                           >  1:338606/1‑149 (MQ=255)

                                                                                                      cAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGccc                                          >  4:96818/1‑148 (MQ=255)

                                                                                                          gTGGGGCATGTCGATACCTGTTGAACAGCGTGTGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGaa                                     <  7:286559/149‑1 (MQ=255)

                                                                                                                  tGTCTATAGATGTTGTACCGCTTGCGCCCCTGGCGCACTGCAAGGTTGTGGAAAGCTCGTTTAGCGGTAGGCGCCGCGCAAACCGGGCACGCGACCGTTCAGGAAAACGTGGTCCAGCAGACCAATGAGCAGGGCCCAGAAAGCCCCg                              <  3:263393/148‑1 (MQ=255)

                                                                                                                    tCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAg                                                                            >  1:315257/1‑100 (MQ=255)

                                                                                                                    tCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAg                                                                            <  2:315257/100‑1 (MQ=255)

                                                                                                                           cTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATAccc                     >  1:43289/1‑148 (MQ=255)

                                                                                                                            tGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCaa                   >  1:262389/1‑149 (MQ=255)

                                                                                                                            tGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCaa                   >  3:345627/1‑149 (MQ=255)

                                                                                                                             gTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCaa                   >  8:193588/1‑148 (MQ=255)

                                                                                                                             gTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCaa                   >  3:92613/1‑148 (MQ=255)

                                                                                                                              ttGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCaa                   >  7:189085/1‑147 (MQ=255)

                                                                                                                              ttGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAAc                  <  1:2321/148‑1 (MQ=255)

                                                                                                                                 accAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAACGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGACAGCCCCGCCAATACCCACCAGGc              >  4:138860/3‑149 (MQ=255)

                                                                                                                                 aaCAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGc                                                                                          >  4:315745/1‑73 (MQ=255)

                                                                                                                                 aaCAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGc                                                                                          <  3:315745/73‑1 (MQ=255)

                                                                                                                                 aaCAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCa                                                                             >  4:308533/1‑86 (MQ=255)

                                                                                                                                 aaCAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAgg               <  2:262389/148‑1 (MQ=255)

                                                                                                                                 aaCAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAgg               <  1:220563/148‑1 (MQ=255)

                                                                                                                                    gccGTGCGCCAGGGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATa                        >  2:242947/3‑136 (MQ=255)

                                                                                                                                    gccGTGCGCCAGGGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATa                        <  1:242947/134‑1 (MQ=255)

                                                                                                                                    aGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAgg               <  3:360194/145‑1 (MQ=255)

                                                                                                                                    aGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAgg               >  4:360194/1‑145 (MQ=255)

                                                                                                                                     gCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGAt          <  4:345627/149‑1 (MQ=255)

                                                                                                                                     ccGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTg                                                                   <  3:34792/91‑1 (MQ=255)

                                                                                                                                     ccGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTg                                                                   >  4:34792/2‑92 (MQ=255)

                                                                                                                                      cGTGCGCCAGGGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGAt          >  3:211470/1‑148 (MQ=255)

                                                                                                                                      cGTGCGCCAGGGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGATg         >  2:178499/1‑149 (MQ=255)

                                                                                                                                        tGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGATGtt       >  7:206314/1‑149 (MQ=255)

                                                                                                                                         gcgcCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGATGTTg      >  6:56982/1‑149 (MQ=255)

                                                                                                                                          cgcCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGATGTTg      <  3:288976/148‑1 (MQ=255)

                                                                                                                                          cgcCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGATGTTg      <  3:205296/148‑1 (MQ=255)

                                                                                                                                          cgcCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGATGTTg      <  4:279586/148‑1 (MQ=255)

                                                                                                                                             cccTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGATGTTGgccg  >  2:133805/1‑149 (MQ=255)

                                                                                                                                             cccTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGATGTTGgccg  >  3:58492/1‑149 (MQ=255)

                                                                                                                                             cccTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGATGTTGgccg  >  1:158649/1‑149 (MQ=255)

                                                                                                                                             cccTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGAACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGATGTTGgcc   >  7:140893/1‑148 (MQ=255)

                                                                                                                                             cccTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGAACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGATGTTGgcc   <  8:140893/148‑1 (MQ=255)

CGCCCAGCAGGCCGGGCAGCACGGGCAGTAGCGCCGGGATCAGCATGATGTGCACATGGCTGACGAAGTGCACGGCAGCAATTTGGGTCAGCAGCTTGGGGCCAGCGTGGGGCATGTCGATACCTGTTGAACAGCGTGCGCCCCTGGCGCACGGCAAGGTGGTGGAAAGCTCGCTTAGCGGGAGGCGCCGCGCAAACCGGGCAGGCGACCGTTCAGGACAACGTGGGCCAGCAGACCAATGACCAGGCCCCAGAAAGCCCCGCCAATACCCAACAGGCTGATGTTGGCCG  >  NC_002947/2312699‑2312988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`