| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 196,351 | C→G | 30.0% | T619T (ACC→ACG) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 196,351 | 0 | C | G | 30.0% | 121.4 / 76.1 | 90 | T619T (ACC→ACG) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (29/33); new base G (14/13); total (44/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-01 | |||||||||||
GACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC > NC_002947/196204‑196496 | gACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCa > 1:28393/1‑149 (MQ=39) cGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTAcc < 5:44885/149‑1 (MQ=39) gATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTAcc > 8:125708/1‑148 (MQ=39) gATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCg > 3:268158/1‑149 (MQ=39) aTCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGt > 5:37168/1‑149 (MQ=39) tCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGtt > 1:101687/1‑149 (MQ=39) aCGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGaa < 8:55732/149‑1 (MQ=39) cGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCg < 6:268563/143‑1 (MQ=39) gACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCCACCCAATACCGTTGAAGc > 8:294069/1‑149 (MQ=255) tCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCaag > 3:330081/1‑148 (MQ=38) accacTACCGCGACCCTGACGGCGAGCCCGTCCGTCACCGAAGGCGCAGTGATAACTTACACAGTGACCCTCAGCAATCCTGCCCAGACGCCGGTGA‑CACT‑‑‑GACCCTGTCCAACGGCCAAACAAATACAGTTGAAGCACGCAAGAcc > 6:19728/1‑147 (MQ=11) ccacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAg < 7:125708/149‑1 (MQ=17) ccacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAg < 8:176725/149‑1 (MQ=17) cacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAgg > 3:167938/1‑149 (MQ=17) cacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAgg < 2:101687/149‑1 (MQ=37) cacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCAAAACCATTACCGTTGAAGCCGGCAAGACCCAgg > 5:124020/1‑149 (MQ=17) acTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAggg < 8:143229/149‑1 (MQ=17) ccaCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAggg > 4:331117/3‑148 (MQ=2) gcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGt > 8:217905/1‑149 (MQ=12) cccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGac > 4:153434/1‑148 (MQ=12) ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGAtt > 5:159268/1‑149 (MQ=34) tGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTc > 3:265161/1‑149 (MQ=34) aCGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTcc < 2:16362/148‑1 (MQ=34) aCGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCa < 4:268158/149‑1 (MQ=34) aCGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTcc < 2:16935/148‑1 (MQ=255) aCGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTc > 5:359657/1‑147 (MQ=255) ggCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGa < 1:216864/149‑1 (MQ=34) cGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAAc < 5:363233/124‑1 (MQ=35) cGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAAc > 6:363233/1‑124 (MQ=35) gAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGAccc > 5:353304/1‑149 (MQ=34) cccGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTcc < 5:32850/140‑1 (MQ=34) cccGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTcc > 6:32850/1‑140 (MQ=34) ccGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGc < 7:20107/149‑1 (MQ=34) gTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGAcc > 6:250501/1‑142 (MQ=255) gTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGAcc < 5:250501/142‑1 (MQ=255) gTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGGAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGa > 5:315193/1‑149 (MQ=11) cACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACg > 3:300735/1‑149 (MQ=255) aCCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGt < 4:265161/149‑1 (MQ=34) aCCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGt > 7:227612/1‑149 (MQ=34) aCCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGt > 6:274722/1‑149 (MQ=255) ccGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTc < 4:5990/149‑1 (MQ=34) ccGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTc < 3:153434/149‑1 (MQ=255) cGAAGGCGGCGTTATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGG‑‑‑GACCCTGTCCAACGGACAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCt < 8:310613/149‑1 (MQ=11) cGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTc > 6:98259/1‑148 (MQ=34) cGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTc > 2:56552/1‑148 (MQ=34) cGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTc > 1:74615/1‑148 (MQ=255) cGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCt < 2:74615/149‑1 (MQ=255) gtaGGCTGCGTGATCACTTACACCGTGACCCTGAGCATCCCTGCCCAGACGCCGTTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTa < 2:292637/147‑1 (MQ=11) ggcggcGTGATCACTTACAGCGTGACCCTGAGCAATCCTGGCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGTCAATACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTacaa < 5:19728/149‑1 (MQ=255) ggcggcGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTacaa < 6:159268/149‑1 (MQ=34) gcggcgTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTacaac < 6:353304/149‑1 (MQ=34) gcgTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACaacaac < 7:294069/149‑1 (MQ=34) cgTGATCACTTACACCGTGACCCTGAGCAACCCTCCCCAGACGCCGGTGACCCCTCAGGACGACACCCACTCGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTacaac < 1:116469/149‑1 (MQ=2) tGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGt < 8:227612/149‑1 (MQ=34) gATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGt < 6:123972/148‑1 (MQ=34) aTCACTTACACCGTGTCCCTGAGCAATCCTGCGCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGtt < 4:204359/148‑1 (MQ=21) tCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTc < 3:110704/148‑1 (MQ=255) aCTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGa < 6:37168/148‑1 (MQ=34) cTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACt < 3:331117/149‑1 (MQ=255) ttACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTg > 2:350679/1‑149 (MQ=255) ttACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTg > 3:120530/1‑149 (MQ=255) acacCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGt > 8:149253/1‑148 (MQ=34) tGACCCTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTc < 4:300735/149‑1 (MQ=11) ccTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTaca < 4:55142/122‑1 (MQ=32) ccTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTaca > 3:55142/1‑122 (MQ=32) ccTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCa < 2:28393/149‑1 (MQ=37) ccTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTacaa > 5:42074/1‑123 (MQ=255) ccTGAGCAACCCTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTacaa < 6:42074/123‑1 (MQ=255) tGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTacaa < 3:326413/121‑1 (MQ=32) tGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTacaa > 4:326413/1‑121 (MQ=32) tGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCAt > 6:70854/1‑148 (MQ=37) cAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTc > 3:245993/1‑112 (MQ=32) cAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTc < 4:245993/112‑1 (MQ=32) aaTCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑‑ACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAAc > 3:284074/1‑149 (MQ=37) cccTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTaca < 1:204218/112‑1 (MQ=255) cccTGCCCAGACGCCGGTGA‑CCGT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTaca > 2:204218/2‑113 (MQ=255) ccAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACcg > 8:60750/1‑149 (MQ=38) ggTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACcggcg < 3:5425/143‑1 (MQ=39) ggTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACcggcg > 4:5425/1‑143 (MQ=39) cgT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGAGTTCCAGACCCCGGCGAATGAc < 6:42867/84‑1 (MQ=255) cgT‑‑‑GACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGAc > 5:42867/2‑85 (MQ=255) t‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGa < 7:287185/145‑1 (MQ=37) t‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGa > 8:287185/1‑145 (MQ=37) aCCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACt > 6:21754/1‑149 (MQ=37) aCCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACt < 7:149253/149‑1 (MQ=37) aaCGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGa < 7:293291/134‑1 (MQ=37) aaCGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGa > 8:293291/1‑134 (MQ=37) aaCGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGaa < 1:56552/149‑1 (MQ=37) ccAAACCATTACCTTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACTGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGa < 5:98259/149‑1 (MQ=255) aaCCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGc > 3:124852/1‑149 (MQ=39) | GACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGA‑CAGT‑‑‑GACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC > NC_002947/196204‑196496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |