BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,380,748	G→A	6.2%	G138S (GGC→AGC)	oprF →	porin F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,380,748	0	G	A	6.2%	342.7 / 8.0	113	G138S (GGC→AGC)	oprF	porin F
Reads supporting (aligned to +/- strand): ref base G (53/53); new base A (4/3); total (57/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01

Only 100 of 123 total aligned reads displayed.

CTCGAACACTGCCCTGGACGCCGTTTACCACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGAACTTCGGCGGTAGCCCGAAGCAAGCTGAAGCTGCT  >  NC_002947/2380600‑2380894

cTCGAACACTGCCCTGGACGCCGTTTACCACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTtggcgctg                                                                                                                                                    >  4:306487/1‑149 (MQ=255)

 tCGAACACTGCCCTGGACGCCGTTTACCACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTtggcgctgg                                                                                                                                                   >  6:176691/1‑149 (MQ=255)

   gAACACTGCCCTGGACGCCGTTTACCACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTtggcgctgg                                                                                                                                                   <  3:228150/147‑1 (MQ=255)

   gAACACTGCCCTGGACGCCGTTTACCACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTtggcgctgg                                                                                                                                                   <  6:327588/147‑1 (MQ=255)

   gAACACTGCCCTGGACGCCGTTTACCACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCATGACCACTCCACCTTCGCCAACGTtggcgctggc                                                                                                                                                  <  6:45558/148‑1 (MQ=255)

     acacTGCCCTGGACGCCGTTTACCACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGcc                                                                                                                                               <  1:139055/149‑1 (MQ=255)

         tGCCCTGGACGCCGTTTACCACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGt                                                                                                                                           >  3:161113/1‑149 (MQ=255)

           cccTGGACGCCGTTTACCACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCCAGCGCCaacgt                                                                                                                                          >  3:217063/1‑145 (MQ=255)

           cccTGGACGCCGTTTACCACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCCAGCGCCaacgt                                                                                                                                          <  4:217063/148‑4 (MQ=255)

           cccTGGACGCCGTTTACCACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCCAGCGCCaacgt                                                                                                                                          <  1:91238/148‑4 (MQ=255)

            ccTGGACGCCGTTTACCACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTgg                                                                                                                                         >  6:227977/1‑148 (MQ=255)

                   gCCGTTTACCACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCAc                                                                                                                                 <  3:89383/149‑1 (MQ=255)

                      gTTTACCACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGa                                                                                                                              >  3:97652/1‑149 (MQ=255)

                         tACCACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACa                                                                                                                            <  8:237044/148‑1 (MQ=255)

                          aCCACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACAt                                                                                                                           <  5:227977/148‑1 (MQ=255)

                            cACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACGAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCCAGCGCCaacgttg                                                                                                                                        <  1:72753/133‑6 (MQ=255)

                            cACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGtt                                                                                                                        <  2:334825/149‑1 (MQ=255)

                            cACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGt                                                                                                                         >  6:344115/1‑148 (MQ=255)

                             aCTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTc                                                                                                                       <  7:318774/149‑1 (MQ=255)

                                 caacaaCCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTAc                                                                                                                                      >  2:202818/1‑130 (MQ=255)

                                 caacaaCCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTAc                                                                                                                                      <  1:202818/130‑1 (MQ=255)

                                 caacaaCCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGGCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTa                                                                                                                     <  8:345871/147‑1 (MQ=255)

                                  aacaacCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGGCCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGtt                                                                                                                        <  4:147714/143‑1 (MQ=255)

                                  aacaacCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGGCCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGtt                                                                                                                        >  3:147714/1‑143 (MQ=255)

                                  aacaacCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGCCCACTCCACCTTCGCCAACGTTGGCGGTGGCGGCAAGTGGTACATCACCGACATGTTGTATGc                                                                                                                  >  4:1839/1‑149 (MQ=255)

                                  aacaacCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGAACATCACCGACATGTTCTACGc                                                                                                                  >  7:244892/1‑149 (MQ=255)

                                   acaacCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTg                                                                                                                                          >  7:150534/1‑124 (MQ=255)

                                   acaacCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTg                                                                                                                                          <  8:150534/124‑1 (MQ=255)

                                    caacCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTgg                                                                                                                                         <  4:178868/124‑1 (MQ=255)

                                    caacCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTgg                                                                                                                                         >  3:178868/1‑124 (MQ=255)

                                    caacCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGccc                                                                                                                <  5:291258/149‑1 (MQ=255)

                                          gTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCg                                                                                                          <  7:34069/149‑1 (MQ=255)

                                               acgCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTa                                                                                                     >  5:155120/1‑149 (MQ=255)

                                                 gCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGccc                                                                                                                <  6:177382/136‑1 (MQ=255)

                                                 gCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGccc                                                                                                                >  5:177382/1‑136 (MQ=255)

                                                  cTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGaa                                                                                                  <  2:88741/149‑1 (MQ=255)

                                                    aTCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGc                                                                                                >  6:135354/1‑149 (MQ=255)

                                                     tCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTACGCCGGCACGGGCATAGaaca                                                                                                <  4:161113/148‑3 (MQ=255)

                                                       cctCCATCCGTTACCGCGGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTACGCCGGCACGGGCATAGaaca                                                                                                <  8:244892/144‑3 (MQ=255)

                                                        gTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAg                                                                                            >  5:168040/1‑149 (MQ=255)

                                                        gTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAg                                                                                            >  5:315950/1‑149 (MQ=255)

                                                         tCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAg                                                                                            >  5:58600/1‑148 (MQ=255)

                                                            aTACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTac                                                                                         >  2:206523/1‑148 (MQ=255)

                                                               cGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTacaaca                                                                                     <  4:346724/149‑1 (MQ=255)

                                                                gTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGt                                                                                                                                        >  4:67441/1‑97 (MQ=255)

                                                                gTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTAc                                                                                                                                      <  6:333246/99‑1 (MQ=255)

                                                                gTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTAc                                                                                                                                      >  5:333246/1‑99 (MQ=255)

                                                                gTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTacaa                                                                                       >  3:218251/1‑146 (MQ=255)

                                                                gTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTacaa                                                                                       <  4:218251/146‑1 (MQ=255)

                                                                gTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACAt                                                                                    >  8:2566/1‑149 (MQ=255)

                                                                gTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACAt                                                                                    >  5:134762/1‑149 (MQ=255)

                                                                gTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACAt                                                                                    >  1:226434/1‑149 (MQ=255)

                                                                gTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAAAGTTGGCGCTGGCGCCAAGTGGt                                                                                                                                        <  3:67441/97‑1 (MQ=255)

                                                                     cGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGAcc                                                                               >  4:307081/1‑149 (MQ=255)

                                                                     cGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGAc                                                                                <  6:315950/148‑1 (MQ=255)

                                                                      gCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAg                                                                                                 <  7:293351/130‑1 (MQ=255)

                                                                      gCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAg                                                                                                 >  8:293351/1‑130 (MQ=255)

                                                                      gCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGAc                                                                                >  4:221824/1‑147 (MQ=255)

                                                                          gTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGc                                                                          <  3:33876/149‑1 (MQ=255)

                                                                          gTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGc                                                                          <  3:307081/149‑1 (MQ=255)

                                                                          gTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGc                                                                          >  3:191249/1‑149 (MQ=255)

                                                                           tttCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGt                                                                                                      <  5:85244/120‑1 (MQ=255)

                                                                           tttCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGt                                                                                                      >  6:85244/1‑120 (MQ=255)

                                                                              ctcGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGac                                                                       <  5:135354/148‑1 (MQ=255)

                                                                               tcGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGacac                                                                     >  2:192338/1‑149 (MQ=255)

                                                                               tcGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGacac                                                                     >  6:119600/1‑149 (MQ=255)

                                                                                  cACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACCTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGACCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGacaac                                                                    >  4:156008/1‑145 (MQ=255)

                                                                                   aCCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAg                                                                 >  7:250840/1‑149 (MQ=255)

                                                                                   aCCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGAAATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAg                                                                 <  5:253027/149‑1 (MQ=255)

                                                                                    ccAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGt                                                                <  7:2566/149‑1 (MQ=255)

                                                                                    ccAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGt                                                                <  2:226434/149‑1 (MQ=255)

                                                                                     cAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTa                                                                                                     <  5:99276/111‑1 (MQ=255)

                                                                                     cAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTa                                                                                                     >  6:99276/1‑111 (MQ=255)

                                                                                     cAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTg                                                               <  5:344115/149‑1 (MQ=255)

                                                                                          gCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGccc                                                          <  3:306487/149‑1 (MQ=255)

                                                                                                 cAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGt                                                   <  3:1839/149‑1 (MQ=255)

                                                                                                  aGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCATGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGtt                                                  >  4:85687/1‑149 (MQ=255)

                                                                                                      cGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTAAGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGgtg                                              >  1:100443/1‑149 (MQ=255)

                                                                                                         ccGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTg                                           <  5:119600/149‑1 (MQ=255)

                                                                                                           gTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCGGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGc                                         >  3:223535/1‑149 (MQ=255)

                                                                                                           gTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGc                                         <  6:168040/149‑1 (MQ=255)

                                                                                                              gCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTg                                      <  4:97652/149‑1 (MQ=255)

                                                                                                                ggTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGa                                     >  2:338920/1‑148 (MQ=255)

                                                                                                                    gTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGAACTTc                                <  3:221824/149‑1 (MQ=255)

                                                                                                                    gTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGAACTTc                                >  7:211389/1‑149 (MQ=255)

                                                                                                                      gACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACAAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGAACTTcgg                              >  3:355041/1‑149 (MQ=255)

                                                                                                                       aCCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGc                                         <  8:261677/137‑1 (MQ=255)

                                                                                                                        ccACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGAACTTcggcg                            <  7:153837/149‑1 (MQ=255)

                                                                                                                         cACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGAACTTcggcgg                           >  4:303123/1‑149 (MQ=255)

                                                                                                                         cACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGAACTTcggcgg                           <  8:211389/149‑1 (MQ=255)

                                                                                                                           cTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGAACTTcggcgg                           >  1:336737/1‑147 (MQ=255)

                                                                                                                           cTCCACCTTCGCCAACGGTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGAACTTCGGCGGTa                         <  8:156862/149‑1 (MQ=255)

                                                                                                                            tCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGAACTTCGGCGGTAg                        >  4:269677/1‑149 (MQ=255)

                                                                                                                              cACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGAACTTCGGCGGTAGcc                      >  1:278377/1‑149 (MQ=255)

                                                                                                                                    cGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTACGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGAACTTCGGCGGTAGCCCGaa                  <  2:100443/147‑1 (MQ=255)

                                                                                                                                       cAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGAACTTCGGCGGTAGCCCGaagcaag             <  4:191249/149‑1 (MQ=255)

                                                                                                                                          acttGGCGCCAGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGAACTTCGGCGGTAGCCCGAAGCaagct           >  2:92838/3‑148 (MQ=255)

                                                                                                                                           gTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGAACTTCGGCGGTAGCCCGAAGCaagctg          >  5:18947/1‑148 (MQ=255)

                                                                                                                                                  ctggcgcCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGAACTTCGGCGGTAGCCCGAAGCAAGCTGAAgctgct  <  7:305739/149‑1 (MQ=255)

                                                                                                                                                  ctggcgcCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGAACTTCGGCGGTAGCCCGAAGCAAGCTGAAgctgct  <  1:338920/149‑1 (MQ=255)

CTCGAACACTGCCCTGGACGCCGTTTACCACTTCAACAACCCGTACGACGCTATCCGTCCATACGTTTCCGCTGGTTTCTCGCACCAGTCGCTGGGCCAGACCGGCCGTGGCGGTCGTGACCACTCCACCTTCGCCAACGTTGGCGCTGGCGCCAAGTGGTACATCACCGACATGTTCTATGCCCGTGCCGGCGTAGAAGCTCAGTACAACATCGACCAGGGCGACACCGAGTGGGCCCCGAGCGTTGGTGTTGGCCTGAACTTCGGCGGTAGCCCGAAGCAAGCTGAAGCTGCT  >  NC_002947/2380600‑2380894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`