BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,388,667	T→G	5.8%	noncoding (101/130 nt)	PP_mr31 ←	rmf regulatory region

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,388,667	0	T	G	5.8%	312.9 / 9.4	104	noncoding (101/130 nt)	PP_mr31	rmf regulatory region
Reads supporting (aligned to +/- strand): ref base T (53/45); new base G (3/3); total (56/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.25e-01

Only 100 of 108 total aligned reads displayed.

GAAGGAAGATTGAAGGGGCAAAGTTCGCGGGATTTGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACGGTGTGAATGACCGATTTGTCATAAGCACGTAA  >  NC_002947/2388519‑2388813

gaaggaagATTGAAGGGGCAAAGTTCGCGGGATTTGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCctct                                                                                                                                                    <  2:161044/149‑1 (MQ=255)

 aaggaagATTGAAGGGGCAAAGTTCGCGGGATTTGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCtctc                                                                                                                                                   >  4:31394/1‑149 (MQ=255)

   ggaagATTGAAGGGGCAAAGTTCGCGGGATTTGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTGCg                                                                                                                                                 >  8:212280/1‑149 (MQ=255)

    gaagATTGAAGGGGCAAAGTTCGCGGGATTTGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTcc                                                                                                                                                  >  4:279392/1‑147 (MQ=255)

    gaagATTGAAGGGGCAAAGTTCGCGGGATTTGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTcc                                                                                                                                                  >  5:204476/1‑147 (MQ=255)

     aagATTGAAGGGGCAAAGTTCGCGGGATTTGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAg                                                                                                                                               <  3:120509/149‑1 (MQ=255)

            aaGGGGCAAAGTTCGCGGGATTTGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCTACGGATCAC‑GCTTATGTCTTCTCATTGGGTGTTGCCCTCACTTGTTGACTGTAATGTCGCGTCGGCCTCTCCGAGGCCggg                                                                                                                                         <  6:337172/148‑1 (MQ=255)

            aaGGGGCAAAGTTCGCGGGATTTGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCggg                                                                                                                                         >  7:331206/1‑148 (MQ=255)

            aaGGGGCAAAGTTCGCGGGATTTGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCggg                                                                                                                                         <  3:279392/148‑1 (MQ=255)

            aaGGGGCAAAGTTCGCGGGATTTGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCggg                                                                                                                                         >  8:196159/1‑148 (MQ=255)

            aaGGCGCAAAGTTCGCGGGATTTGCCGGTCACCCCGTATTGTCAGCCACGTTAATATGCTCTTTCCAACGCATCAC‑GCTTAAGTCTTCTCATCGGGTGTTGCCCTCACTTGTTCACTGTAATGCCCCGGCGGCCTCGGAGAGGCCgacg                                                                                                                                        <  4:284732/149‑4 (MQ=255)

               gggCAAAGTTCGCGGGATTTGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCCGGCag                                                                                                                                      >  2:133245/1‑148 (MQ=255)

                 gCAAAGTTCGCGGGATTTGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGATTACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGt                                                                                                                                   >  1:302336/1‑149 (MQ=255)

                   aaaGTTCGCGGGATTTGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCg                                                                                                                                 >  3:190914/1‑149 (MQ=255)

                   aaaGTTCGCGGGATTTGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGCCGGCCTCGGAGAGGCCgacggg                                                                                                                                      >  1:213970/1‑139 (MQ=255)

                   aaaGTTCGCGGGATTTGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGCCGGCCTCGGAGAGGCCgacggg                                                                                                                                      <  2:213970/144‑6 (MQ=255)

                       ttCGCGGGATTTGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCACGTCGGCCACTCCGAGGCCGGGCACAGTCGTTCt                                                                                                                             >  4:18297/1‑149 (MQ=255)

                               aTTTGCCGGTGACCCCGTATTGGTAGCCACTTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGttt                                                                                                                     <  3:28233/149‑1 (MQ=255)

                                  tGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTgc                                                                                                                   <  4:190914/148‑1 (MQ=255)

                                      ggTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGt                                                                                                                       <  3:256437/140‑1 (MQ=255)

                                      ggTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGt                                                                                                                       >  4:256437/1‑140 (MQ=255)

                                            cccGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCg                                                                                                        >  3:80146/1‑149 (MQ=255)

                                            cccGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCg                                                                                                        >  3:189358/1‑149 (MQ=255)

                                             ccGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTc                                                                                                                                  <  6:83419/122‑1 (MQ=255)

                                             ccGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTAGCTGCCgg                                                                                                       >  5:25869/1‑149 (MQ=255)

                                              cGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTgcgc                                                                                                                 >  5:263158/1‑138 (MQ=255)

                                              cGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTgcgc                                                                                                                 <  6:263158/138‑1 (MQ=255)

                                               gTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCg                                                                                                     >  8:192021/1‑149 (MQ=255)

                                                    ggTAGCCACGTGAATATGCTCTTTCCAACGGATCACTGCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGc                                                                                                 >  1:59170/1‑149 (MQ=255)

                                                    ggTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGc                                                                                                 <  6:204476/148‑1 (MQ=255)

                                                      tAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAg                                                                                              >  3:342915/1‑149 (MQ=255)

                                                         ccACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCt                                                                                            >  2:225852/1‑148 (MQ=255)

                                                             gTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTTAAAg                                                                                       >  2:295989/1‑149 (MQ=255)

                                                              tGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGt                                                                                      <  4:80146/149‑1 (MQ=255)

                                                                 atatGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCt                                                                                            >  5:263857/1‑140 (MQ=255)

                                                                 atatGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCt                                                                                            <  6:263857/140‑1 (MQ=255)

                                                                  tatGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCGCTACCAGGCCGGGGAGAGTCGTTGTGGCGGGTTTCGCGAGCGTCCGGCGGGGCGAGCAGaaaattccc                                                                                  >  6:235253/1‑143 (MQ=255)

                                                                    tGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTgccgcc                                                                                >  5:204438/1‑149 (MQ=255)

                                                                     gCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCt                                                                               >  1:133257/1‑149 (MQ=255)

                                                                      ctctTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTc                                                                              >  7:176626/1‑149 (MQ=255)

                                                                      ctctTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTc                                                                              >  2:49352/1‑149 (MQ=255)

                                                                       tctTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCg                                                                             >  2:250281/1‑149 (MQ=255)

                                                                            ccAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGc                                                                         <  1:225852/148‑1 (MQ=255)

                                                                            ccAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGc                                                                         >  1:136673/1‑148 (MQ=255)

                                                                             cAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCg                                                                        >  6:283684/1‑148 (MQ=255)

                                                                             cAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCg                                                                        >  6:68080/1‑148 (MQ=255)

                                                                             cAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCg                                                                        >  6:261658/1‑148 (MQ=255)

                                                                             cAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCACTCCGAGGCCGGGCAGAGTGGTTGTGCCGGTTTTCGCTCGCTGCCCGCGTGGGGGGCTGAAAGTGCCGCCTCGTTggg                                                                        >  6:35590/1‑146 (MQ=255)

                                                                              aaCGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGGCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCg                                                                        >  1:295202/1‑147 (MQ=255)

                                                                              aaCGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCg                                                                        >  3:122237/1‑147 (MQ=255)

                                                                               aCGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCGCGTTGcata                                                                      >  2:161632/1‑145 (MQ=255)

                                                                                cGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGc                                                                    <  2:59170/149‑1 (MQ=255)

                                                                                 ggATCAC‑GCTTAAGTCTTCTCCTAGGCGGGTGCCCTCACTTGTTGACTGTACTGTCCCGTCGGCCTCTCCGCGGCCGGGCAGAGTCGTTCTACCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCg                                                                   <  8:124338/149‑1 (MQ=255)

                                                                                   aTCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGc                                                                 <  6:282844/149‑1 (MQ=255)

                                                                                    tCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCTTCGCAACGAggcggca                                                                <  3:171806/149‑2 (MQ=255)

                                                                                     cAC‑GCTTAAGGCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGCCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGGTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAACTGCCGCTTCGCAACGAggcggcac                                                               <  7:55106/149‑3 (MQ=255)

                                                                                      aC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGa                                                                       >  1:224661/1‑140 (MQ=255)

                                                                                      aC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGa                                                                       <  2:224661/140‑1 (MQ=255)

                                                                                       c‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTga                                                             <  2:136673/149‑1 (MQ=255)

                                                                                         gCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTgag                                                            <  2:295202/149‑1 (MQ=255)

                                                                                          cTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTgaga                                                           >  7:142422/1‑149 (MQ=255)

                                                                                           ttAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTGTCCGAGGCCGGGCAGAGTCGTAATGCGGCTTTTCCCTACCTGCCGGCGTGGCGAGCTGAAACTGCGGCCTCGTTGGGAAGCGGCCTga                                                             >  8:105923/1‑146 (MQ=255)

                                                                                           ttAAGTCATCTCATAGGGTGTTGCCCTGACTTGTTGACTGTAATCTGCCGTCGGCCTCTCCCAGGCCGGGCAGAGTCGTTCTGCCGGATTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTCCCGCCTCGTTGCGAAGCGGCCTga                                                             <  7:105923/146‑1 (MQ=255)

                                                                                               gTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGt                                                      <  5:235253/149‑1 (MQ=255)

                                                                                                tcttctCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGtt                                                     <  1:250281/149‑1 (MQ=255)

                                                                                                   tctcATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCt                                                   <  4:122237/148‑1 (MQ=255)

                                                                                                     tcATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGtt                                                     >  4:325947/1‑144 (MQ=255)

                                                                                                     tcATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGtt                                                     <  3:325947/144‑1 (MQ=255)

                                                                                                     tcATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATc                                                >  1:127516/1‑149 (MQ=255)

                                                                                                     tcATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGATGCGAAGCGGCCTGAGACCAGTTCTATc                                                >  7:169436/1‑149 (MQ=255)

                                                                                                       aTAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATc                                                <  7:192021/147‑1 (MQ=255)

                                                                                                       aTAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCg                                               >  5:132708/1‑148 (MQ=255)

                                                                                                        tAGGGTGTTGCCCTCTCTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCCCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGaa                                             <  3:18297/149‑1 (MQ=255)

                                                                                                             tgtTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGa                                        <  2:127516/149‑1 (MQ=255)

                                                                                                             tgtTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGa                                        <  8:142422/149‑1 (MQ=255)

                                                                                                               ttGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGt                                      <  2:133257/149‑1 (MQ=255)

                                                                                                                   ccTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACg                                  <  4:189358/149‑1 (MQ=255)

                                                                                                                    cTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACgg                                 >  8:14760/1‑149 (MQ=255)

                                                                                                                    cTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACgg                                 <  7:14760/149‑1 (MQ=255)

                                                                                                                       aCTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGaa                                                                      <  1:243490/109‑1 (MQ=255)

                                                                                                                       aCTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGaa                                                                      >  2:243490/1‑109 (MQ=255)

                                                                                                                         ttgttgACTGTAATGTCCCGTCGGCCGCTCCGCGGCCCGGCAGAGTGGTTGTGGCGGGTTTCGCGAGCGGCCCGCGGGGGGAGCGGAAAATTCCCCCGCGGTTCGAAGCGGCCGGAGACCACGTCTATAGAATGCGAGTCACCGtgtg                             >  6:250138/1‑148 (MQ=255)

                                                                                                                           gttgACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACGGTGTGa                            <  6:204438/147‑1 (MQ=255)

                                                                                                                           gttgACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACGGTGTGAAt                          >  7:192912/1‑149 (MQ=255)

                                                                                                                            ttgACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACGGTGTGAAt                          <  7:12834/148‑1 (MQ=255)

                                                                                                                            ttgACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACGGTGTGAAt                          <  5:261658/148‑1 (MQ=255)

                                                                                                                              gACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGGGCGCTGAAAGTGCCGCCGCGTTGCGCAGCGGCCTGCGACCAGtt                                                     >  6:225740/1‑119 (MQ=255)

                                                                                                                              gACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACGGTGTGAATGa                        <  5:250138/148‑1 (MQ=255)

                                                                                                                                cTGTAATGTCCCGTCGGCCTCTCCGAGGCCGCGCAGAGTCGTTCTTCCGGTTTGCCCTCGCTGACCGCGTGGCGACCTGAAAGTGCCGCCACGTTGCGAAGCGGCCGGAGACCAGTTCGATAGAATGCGAGTGCCGCTGTGAATGAcc                      >  4:281308/1‑148 (MQ=255)

                                                                                                                                        tCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACGGTGTGAATGACCGATTTGTc              >  8:17371/1‑148 (MQ=255)

                                                                                                                                        tCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACGGTGTGAATGACCGATTTGTc              <  8:176626/148‑1 (MQ=255)

                                                                                                                                        tCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACGGTGTGAATGACCGATTTGTCa             >  5:201167/1‑149 (MQ=255)

                                                                                                                                          ccGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACGGTGTGAATGACCGATTTGTCAt            >  1:126746/1‑148 (MQ=255)

                                                                                                                                          ccGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACGGTGTGAATGACCGATTTGTCAt            >  4:153754/1‑148 (MQ=255)

                                                                                                                                            gTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACGGTGTGAATGACCGATTTGTCATAAg         >  6:258168/1‑149 (MQ=255)

                                                                                                                                            cccGGCCTCGGAGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACGGTGTGAATGACCGATTTGTCATAAg         >  7:219995/3‑149 (MQ=255)

                                                                                                                                             ccGGCCTCGGAGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACGGTGTGAATGACCGATTTGTCATAAg         >  6:331557/2‑148 (MQ=255)

                                                                                                                                               ggCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACGGTGTGAATGACCGATTTGTCATAAGCa       <  5:35590/148‑1 (MQ=255)

                                                                                                                                                gCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTg                                                                          >  5:260445/1‑80 (MQ=255)

                                                                                                                                                gCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTg                                                                          <  6:260445/80‑1 (MQ=255)

GAAGGAAGATTGAAGGGGCAAAGTTCGCGGGATTTGCCGGTGACCCCGTATTGGTAGCCACGTGAATATGCTCTTTCCAACGGATCAC‑GCTTAAGTCTTCTCATAGGGTGTTGCCCTCACTTGTTGACTGTAATGTCCCGTCGGCCTCTCCGAGGCCGGGCAGAGTCGTTCTGCCGGTTTGCGCTCGCTGCCGGCGTGGCGAGCTGAAAGTGCCGCCTCGTTGCGAAGCGGCCTGAGACCAGTTCTATCGAATGCGAGTCACGGTGTGAATGACCGATTTGTCATAAGCACGTAA  >  NC_002947/2388519‑2388813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`