BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,444,034	A→G	10.0%	K184E (AAA→GAA)	PP_2140 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,444,034	0	A	G	10.0%	374.7 / 23.9	139	K184E (AAA→GAA)	PP_2140	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (63/62); new base G (6/8); total (69/70)
Fisher's exact test for biased strand distribution p-value = 7.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 142 total aligned reads displayed.

GCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTGTCGAGTTGCCTGACGGCCGCATCGA  >  NC_002947/2443887‑2444178

gCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTa                                                                                                                                                   >  2:255561/1‑148 (MQ=255)

 cTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTaa                                                                                                                                                  >  2:98458/1‑148 (MQ=255)

 cTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTaa                                                                                                                                                  <  2:89780/148‑1 (MQ=255)

   tATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAg                                                                                                                                               >  2:105264/1‑149 (MQ=255)

    aTGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGt                                                                                                                                              >  5:140074/1‑149 (MQ=255)

       ccGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTCTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTg                                                                                                                                           <  1:255561/149‑1 (MQ=255)

        cGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTg                                                                                                                                           <  8:216012/148‑1 (MQ=255)

        cGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTg                                                                                                                                           >  7:216012/1‑148 (MQ=255)

          aTTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTg                                                                                                                                        >  2:339799/1‑149 (MQ=255)

              gTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGAc                                                                                                                                    >  1:241799/1‑149 (MQ=255)

               ttCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGAc                                                                                                                                    <  3:47727/148‑1 (MQ=255)

               ttCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACg                                                                                                                                   >  7:78983/1‑149 (MQ=255)

               ttCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCGGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGCCg                                                                                                                                   >  7:52553/1‑149 (MQ=255)

                     ccaccGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGTGC‑AATGACCTTAAAAGTTTGGTGAGACGttt                                                                                                                                >  2:331947/1‑146 (MQ=255)

                     ccaccGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGTGC‑AATGACCTTAAAAGTTTGGTGAGACGttt                                                                                                                                <  1:331947/146‑1 (MQ=255)

                      caccGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGt                                                                                                                            >  5:193234/1‑149 (MQ=255)

                      caccGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGt                                                                                                                            <  2:241799/149‑1 (MQ=255)

                        ccGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑a                                                                                                                         <  3:148400/149‑1 (MQ=255)

                           gTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑Acgc                                                                                                                      <  3:19539/149‑1 (MQ=255)

                           gTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑Acgc                                                                                                                      <  6:226540/149‑1 (MQ=255)

                              gCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGtt                                                                                                                   <  8:158189/149‑1 (MQ=255)

                               ccAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTGAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGtt                                                                                                                   >  2:213099/1‑148 (MQ=255)

                               ccAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGtt                                                                                                                   >  6:275932/1‑148 (MQ=255)

                               ccAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGtt                                                                                                                   >  6:183603/1‑148 (MQ=255)

                                  gAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGCAAATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTtgag                                                                                                                <  1:74290/149‑1 (MQ=255)

                                  gAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTtgagt                                                                                                               <  4:203551/149‑1 (MQ=255)

                                     aaGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTtgagtgag                                                                                                            <  1:190441/149‑1 (MQ=255)

                                        cgcCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGc                                                                                                         <  5:275932/149‑1 (MQ=255)

                                           cGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGa                                                                                                      >  6:288238/1‑149 (MQ=255)

                                           cGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGa                                                                                                      <  5:183603/149‑1 (MQ=255)

                                            gAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGttt                                                                                                                                            <  8:257421/111‑1 (MQ=255)

                                            gAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGttt                                                                                                                                            >  7:257421/1‑111 (MQ=255)

                                               aaCCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGc                                                                                                  >  2:15717/1‑149 (MQ=255)

                                                aCCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGcc                                                                                                 <  8:311884/149‑1 (MQ=255)

                                                  cGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCg                                                                                                >  7:77665/1‑148 (MQ=255)

                                                    aCGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCaa                                                                                             <  6:140074/149‑1 (MQ=255)

                                                         aCCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCCTTGAGTGAGTGCTGATAGCCGCAACGGc                                                                                         <  1:205794/148‑1 (MQ=255)

                                                          cccAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTg                                                                                       >  5:208648/1‑149 (MQ=255)

                                                           ccAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTgg                                                                                      >  3:81904/1‑149 (MQ=255)

                                                           ccAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTgg                                                                                      <  1:105264/149‑1 (MQ=255)

                                                             aGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGc                                                                                         <  6:157787/144‑1 (MQ=255)

                                                                 ccTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACaat                                                                                <  6:184378/149‑1 (MQ=255)

                                                                 ccTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACaa                                                                                 >  7:159343/1‑148 (MQ=255)

                                                                  cTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACaat                                                                                >  4:58195/1‑148 (MQ=255)

                                                                     aTTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTGAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATc                                                                            >  1:356345/1‑149 (MQ=255)

                                                                     aTTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTGAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATc                                                                            <  6:46613/149‑1 (MQ=255)

                                                                         aaGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTtgagtga                                                                                                             <  5:294274/112‑1 (MQ=255)

                                                                           gCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCTGACCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCttt                                                                      >  1:74843/1‑149 (MQ=255)

                                                                            cTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTc                                                                     >  3:6260/1‑149 (MQ=255)

                                                                               tCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTcgcg                                                                  >  2:179643/1‑149 (MQ=255)

                                                                               tCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTcgcg                                                                  >  8:180642/1‑149 (MQ=255)

                                                                               tCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTcgcg                                                                  >  8:267077/1‑149 (MQ=255)

                                                                               tCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTcgc                                                                   <  5:24541/148‑1 (MQ=255)

                                                                                  aTCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTGTCCGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGga                                                                <  1:98458/149‑1 (MQ=255)

                                                                                     ttGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGga                                                                >  3:213042/1‑145 (MQ=255)

                                                                                      tgatgaGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACt                                                           >  5:161146/1‑149 (MQ=255)

                                                                                       gatgaGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACt                                                           <  8:37306/148‑1 (MQ=255)

                                                                                        atgaGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATc                                                                            >  2:282401/1‑130 (MQ=255)

                                                                                        atgaGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATc                                                                            <  1:282401/130‑1 (MQ=255)

                                                                                         tgaGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTCAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGcc                                                        <  2:77663/149‑1 (MQ=255)

                                                                                            gCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTGAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCAtt                                                     >  5:293480/1‑149 (MQ=255)

                                                                                                gAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACaa                                                                                 <  7:16118/117‑1 (MQ=255)

                                                                                                 ctcATCCCGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATg                                                 <  8:52553/146‑1 (MQ=255)

                                                                                                 aGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTGAAAGTTTGGTGAGACGTTTCCGTG‑ACTCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTAt                                                  <  6:110760/147‑1 (MQ=255)

                                                                                                 aGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTGAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGt                                                <  6:293480/149‑1 (MQ=255)

                                                                                                 aGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGGAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTAt                                                  <  1:254038/147‑1 (MQ=255)

                                                                                                 aGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTAt                                                  <  6:208648/147‑1 (MQ=255)

                                                                                                 aGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATg                                                 <  4:6260/148‑1 (MQ=255)

                                                                                                  gCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGt                                                <  7:180642/148‑1 (MQ=255)

                                                                                                   cATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTc                                               >  3:310711/1‑148 (MQ=255)

                                                                                                        aGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTc                                         >  5:180143/1‑149 (MQ=255)

                                                                                                           gcTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCttt                                      <  1:339799/149‑1 (MQ=255)

                                                                                                           gcTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAAAGGCTGGTACAATAATCGACTTTCGCGGAGAACTGCCATTTATGTCTACGTCttt                                      >  6:332776/1‑149 (MQ=255)

                                                                                                            cTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCtttt                                     >  7:315779/1‑149 (MQ=255)

                                                                                                            cTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCtttt                                     <  8:315779/149‑1 (MQ=255)

                                                                                                             tGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTc                                               >  5:141796/1‑138 (MQ=255)

                                                                                                                        gAGCTGGAAAGCTGGCGC‑AATGACCTTGAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATATTCGCCTTTCGCGGAGAACTGCCATTTATGTCt                                              >  1:296839/1‑128 (MQ=255)

                                                                                                                        gAGCTGGAAAGCTGGCGC‑AATGACCTTGAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCt                                              <  2:296839/128‑1 (MQ=255)

                                                                                                                        gAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTGTc                         <  6:161146/149‑1 (MQ=255)

                                                                                                                        gAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTATc                         <  3:263431/149‑1 (MQ=255)

                                                                                                                         aGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGc                                                                                         <  1:63946/84‑1 (MQ=255)

                                                                                                                         aGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGc                                                                                         >  2:63946/1‑84 (MQ=255)

                                                                                                                         aGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTAt                                                  <  3:212597/123‑1 (MQ=255)

                                                                                                                         aGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTAt                                                  >  4:212597/1‑123 (MQ=255)

                                                                                                                         aGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTGTc                         >  4:289594/1‑148 (MQ=255)

                                                                                                                          gCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTGTCGa                       <  8:159343/149‑1 (MQ=255)

                                                                                                                            tGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTGTc                         <  4:50326/145‑1 (MQ=255)

                                                                                                                            tGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTGTc                         >  3:50326/1‑145 (MQ=255)

                                                                                                                             ggAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTGTCGAGtt                    >  1:360018/1‑149 (MQ=255)

                                                                                                                              gAAAGCTGGCGC‑AATGACCTTGAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTGTCGAGTTg                   >  7:239851/1‑149 (MQ=255)

                                                                                                                              gAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTGTCGAGTTg                   >  1:10675/1‑149 (MQ=255)

                                                                                                                                 aGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTGTCGAGTTGCCt                <  4:239351/149‑1 (MQ=255)

                                                                                                                                      gcgc‑AATGACCTTGAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGcc                                                        >  8:255396/1‑104 (MQ=255)

                                                                                                                                      gcgc‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTGTCGAGTTGCCTGACgg           >  6:176671/1‑149 (MQ=255)

                                                                                                                                      gcgc‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTGTCGAGTTGCCTGACgg           >  1:127362/1‑149 (MQ=255)

                                                                                                                                       cgc‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTGTCGAGTTGCCTGACGGc          <  2:360018/149‑1 (MQ=255)

                                                                                                                                       cgc‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTGTCGAGTTGCCTGACGGc          <  2:10675/149‑1 (MQ=255)

                                                                                                                                           aaTGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTGTCGAGTTGCCTGACGGCCg        >  5:33692/1‑148 (MQ=255)

                                                                                                                                                 cTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTGTCGAGTTGCCTGACGGcc         >  4:113960/1‑141 (MQ=255)

                                                                                                                                                 cTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTGTCGAGTTGCCTGACGGCCGCATCGa  >  3:221833/1‑148 (MQ=255)

GCTTATGCCGATTTGTTCCGACCACCGGTCGCCAGAAAAGCGCCGAAAACCGACGTTACCCAGCCCCTGATTCAAGCTGTCAATCTTGATGAGCCTGAGCATCCAGCGCTGTCGCGTGCCGAGCTGGAAAGCTGGCGC‑AATGACCTTAAAAGTTTGGTGAGACGTTTCCGTG‑ACGCGTTGAGTGAGTGCTGATAGCCGCAACGGCTGGTACAATAATCGCCTTTCGCGGAGAACTGCCATTTATGTCTACGTCTTTTCTGGAAATTGTCGAGTTGCCTGACGGCCGCATCGA  >  NC_002947/2443887‑2444178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`