Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,457,000 T→A 6.1% L24Q (CTG→CAG)  PP_2150 → thiamine biosynthesis lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,457,0000TA 6.1% 390.1 / 10.4 129L24Q (CTG→CAG) PP_2150thiamine biosynthesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (66/55);  new base A (3/5);  total (69/60)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

Only 100 of 134 total aligned reads displayed.

TACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCATCGT  >  NC_002947/2456854‑2457147
                                                                                                                                                    |                                                                                                                                                   
tACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTgg                                                                                                                                                   >  4:216283/1‑149 (MQ=255)
   aGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGggtg                                                                                                                                                <  3:346780/149‑1 (MQ=255)
   aGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGggtg                                                                                                                                                >  6:14169/1‑149 (MQ=255)
   aGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGggtg                                                                                                                                                <  5:143652/149‑1 (MQ=255)
    gtaTTATCGCGCCCCATGTCGTAGGGGTTTTACCCGCTAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCACACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCAGGCGCTCCAGggtgg                                                                                                                                               <  6:10017/147‑1 (MQ=255)
    ggATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTgg                                                                                                                                                   <  8:153656/145‑1 (MQ=255)
    ggATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTgg                                                                                                                                                   >  7:153656/1‑145 (MQ=255)
     gATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGggtggt                                                                                                                                              >  3:119946/1‑149 (MQ=255)
     gATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGggtggt                                                                                                                                              <  4:346091/149‑1 (MQ=255)
      aTTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCAGGCGCTCCAGggtgggg                                                                                                                                             <  3:26668/149‑3 (MQ=255)
       ttATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTcc                                                                                                                                            >  3:77866/1‑149 (MQ=255)
          tCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGggtgggatagg                                                                                                                                         >  1:128796/1‑143 (MQ=255)
            gCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTgg                                                                                                                                                   <  1:164346/137‑1 (MQ=255)
               gCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATgg                                                                                                                                    >  4:174764/1‑149 (MQ=255)
                ccccTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATggg                                                                                                                                   <  5:14169/149‑1 (MQ=255)
                  ccTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATggg                                                                                                                                   >  7:133554/1‑147 (MQ=255)
                  ccTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATggg                                                                                                                                   >  3:175501/1‑147 (MQ=255)
                   cTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGgcag                                                                                                                                <  3:216283/149‑1 (MQ=255)
                     gTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGgcag                                                                                                                                >  7:284420/1‑147 (MQ=255)
                        ggAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCagcagc                                                                                                                            <  8:283623/148‑1 (MQ=255)
                         gAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTggg                                                                                                                                                  >  3:175521/1‑125 (MQ=255)
                          aGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTAc                                                                                                                         <  7:280276/149‑1 (MQ=255)
                          aGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTAc                                                                                                                         <  2:59541/149‑1 (MQ=255)
                            cGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACa                                                                                                                        <  8:133554/148‑1 (MQ=255)
                             gggTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTggg                                                                                                                                                  >  1:45569/1‑121 (MQ=255)
                             gggTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTggg                                                                                                                                                  <  2:45569/121‑1 (MQ=255)
                              ggTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGTAGCTACAGCa                                                                                                                     >  7:106376/1‑149 (MQ=255)
                              ggTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCa                                                                                                                     <  1:292529/149‑1 (MQ=255)
                                    cccGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAg                                                                                                                >  6:277331/1‑148 (MQ=255)
                                    cccGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAg                                                                                                                <  7:69209/148‑1 (MQ=255)
                                     ccGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGgcagc                                                                                                                               <  1:282166/132‑1 (MQ=255)
                                     ccGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGgcagc                                                                                                                               >  2:282166/1‑132 (MQ=255)
                                     ccGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGt                                                                                                               <  3:133229/148‑1 (MQ=255)
                                     ccGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGt                                                                                                               >  4:133229/1‑148 (MQ=255)
                                       gcgAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACg                                                                                                            >  6:189432/1‑149 (MQ=255)
                                             agGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCcgc                                                                                                       >  2:6146/1‑148 (MQ=255)
                                               gCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAg                                                                                                    >  2:342175/1‑149 (MQ=255)
                                                 cAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGcc                                                                                                  <  6:335751/149‑1 (MQ=255)
                                                              cGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCGGCTGAAACCAAGCCCCCCCCGTGGAGCGCATGGGTGGACCGCCAATGGCCACCAGAAACAGAAACAATAACGACCGCGCCCATGGTGCCCCGc                                                                                       >  1:98787/1‑149 (MQ=255)
                                                               gagaAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGc                                                                                    <  8:284420/149‑1 (MQ=255)
                                                                    aCCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGcc                                                                               <  4:77866/149‑1 (MQ=255)
                                                                     cccTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGc                                                                                <  8:215408/147‑1 (MQ=255)
                                                                     cccTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGc                                                                                >  7:215408/1‑147 (MQ=255)
                                                                       cTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGTAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAg                                                                            >  4:293743/1‑149 (MQ=255)
                                                                       cTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTAGCCCGCTGCCGGCCCAg                                                                            >  1:123108/1‑149 (MQ=255)
                                                                        tACTGTGCGCACAGCCCTACTGTACATTCTGCTGCTACTCACCGC‑‑CTCCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCACCAGCTACAGCAACCAGAACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAgg                                                                           >  7:65444/1‑149 (MQ=255)
                                                                            gtgCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCt                                                                                      >  7:189590/1‑134 (MQ=255)
                                                                            gtgCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCt                                                                                      <  8:189590/134‑1 (MQ=255)
                                                                             tgCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCAACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGc                                                                        <  7:281356/147‑1 (MQ=255)
                                                                                gcACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATg                                                                   <  5:277331/149‑1 (MQ=255)
                                                                                 cacaGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATgg                                                                  <  1:6146/149‑1 (MQ=255)
                                                                                    aGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGcc                                                                               <  7:108373/133‑1 (MQ=255)
                                                                                    aGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGcc                                                                               >  8:108373/1‑133 (MQ=255)
                                                                                     gCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGt                                                              <  6:358423/149‑1 (MQ=255)
                                                                                      cccTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTg                                                             >  2:250282/1‑149 (MQ=255)
                                                                                        cTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTgg                                                            >  5:253585/1‑148 (MQ=255)
                                                                                         tACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCtggt                                                                                              >  5:71533/1‑113 (MQ=255)
                                                                                         tACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCtggt                                                                                              <  6:71533/113‑1 (MQ=255)
                                                                                             gTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCg                                                                                  >  1:134399/1‑121 (MQ=255)
                                                                                             gTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCg                                                                                  <  2:134399/121‑1 (MQ=255)
                                                                                             gTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCa                                                      >  1:313968/1‑149 (MQ=255)
                                                                                              ttCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAAGTGCAGATGGCGGTGGAAACCAt                                                     >  3:238371/1‑149 (MQ=255)
                                                                                                cATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCATCATCTACATCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATc                                                    <  2:214549/148‑1 (MQ=255)
                                                                                                cATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATcc                                                   >  8:164635/1‑149 (MQ=255)
                                                                                                cATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATc                                                    <  3:249637/148‑1 (MQ=255)
                                                                                                     tgctgcTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCAc                                              >  2:232249/1‑149 (MQ=255)
                                                                                                        tgctACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGa                                            >  2:134624/1‑148 (MQ=255)
                                                                                                          ctACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACAt                                         >  8:47342/1‑149 (MQ=255)
                                                                                                             cTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGa                                      >  1:76733/1‑149 (MQ=255)
                                                                                                              tCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCAAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGac                                     >  8:46596/1‑149 (MQ=255)
                                                                                                                aCCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGacca                                   >  4:289189/1‑149 (MQ=255)
                                                                                                                   gC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGAccacca                                >  8:307939/1‑149 (MQ=255)
                                                                                                                        tGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCacta                             >  1:46391/1‑149 (MQ=255)
                                                                                                                         gCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTTCAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCt                                                                                      <  3:117054/91‑1 (MQ=255)
                                                                                                                         gCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCt                                                                                      >  4:117054/1‑91 (MQ=255)
                                                                                                                           aaCCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGa                                      <  6:229797/137‑1 (MQ=255)
                                                                                                                           aaCCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGa                                      >  5:229797/1‑137 (MQ=255)
                                                                                                                           aaCCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTc                          >  4:66068/1‑149 (MQ=255)
                                                                                                                            aCCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTc                          >  1:168924/1‑148 (MQ=255)
                                                                                                                            aCCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCg                         <  2:293946/149‑1 (MQ=255)
                                                                                                                              cAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCa                                               <  8:103690/125‑1 (MQ=255)
                                                                                                                               gtcggaccACCCAGGCGCTCCAGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGAc                       >  4:58953/7‑148 (MQ=255)
                                                                                                                                 cggaccACCCAGGCGCTCCAGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCCCCACTACTCGACCTa                    >  4:286979/5‑149 (MQ=255)
                                                                                                                                    cccACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACcg                 <  3:351059/149‑1 (MQ=255)
                                                                                                                                    accACCCAGGCGCTCCAGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGac                                     <  5:85686/128‑1 (MQ=255)
                                                                                                                                    accACCCAGGCGCTCCAGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGac                                     >  6:85686/2‑129 (MQ=255)
                                                                                                                                     ccACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACcgc                >  8:274882/1‑149 (MQ=255)
                                                                                                                                       aCCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCgcgg              >  1:214923/1‑149 (MQ=255)
                                                                                                                                             gAGCGCCTGGGTGGTCCGACCATGGGCAGTAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGAtt         <  8:106376/148‑1 (MQ=255)
                                                                                                                                             gAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGAtt         <  2:128796/148‑1 (MQ=255)
                                                                                                                                             gAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTc        <  7:47342/149‑1 (MQ=255)
                                                                                                                                              aGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTcc       <  1:232249/149‑1 (MQ=255)
                                                                                                                                               gcgcCTGGGTGGTCCGACCATGGGCAGTAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCa      <  3:293743/149‑1 (MQ=255)
                                                                                                                                                 gcCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCATc    <  1:250282/149‑1 (MQ=255)
                                                                                                                                                 gcCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCATc    <  5:128191/149‑1 (MQ=255)
                                                                                                                                                   cTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCATCg   >  1:203025/1‑148 (MQ=255)
                                                                                                                                                   cTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCATCg   >  6:45246/1‑148 (MQ=255)
                                                                                                                                                    tGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCAc                                              >  3:83924/1‑104 (MQ=255)
                                                                                                                                                    tGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACcg                 <  1:363220/133‑1 (MQ=255)
                                                                                                                                                    tGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCATCGt  >  4:139321/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGC‑‑CTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCATCGT  >  NC_002947/2456854‑2457147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑