Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,474,162	A→G	7.6%	intergenic (‑67/+113)	PP_2167 ← / ← tal	PhoD‑like phosphatase/transaldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,474,162	0	A	G	7.6%	177.3 / 2.6	67	intergenic (‑67/+113)	PP_2167/tal	PhoD‑like phosphatase/transaldolase
Reads supporting (aligned to +/- strand):  ref base A (26/34);  new base G (3/2);  total (30/37)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAAGCTTTTC  >  NC_002947/2474015‑2474310                                                                                                                                                    |                                                                                                                                                       ccagacggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAg                                                                                                                                                       <  2:168588/149‑1 (MQ=255)   agacggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGATCGATGTAAGGGCg                                                                                                                                                     <  1:181889/149‑1 (MQ=37)    gacggccatacgCTGGGGTTCCAGGCGGGGGAGTACCGGGCCGGCGAGTACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGGAAGCCCGCTCCTACACTGATCGATGTAAGGGCgg                                                                                                                                                    <  3:215329/149‑1 (MQ=25)      cggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGTGCCTGCGAGAACGAGGGGCAACGGGTTAGGTGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCggg                                                                                                                                                   <  7:171554/148‑1 (MQ=255)      cggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGTTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGc                                                                                                                                                  <  1:281669/149‑1 (MQ=255)      cggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGATCGATGTAAgggcgggc                                                                                                                                                  <  7:103282/149‑1 (MQ=37)        gccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCtt                                                                                                                                                <  2:214096/149‑1 (MQ=255)         ccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑g                                                                                                                                              <  1:104736/149‑1 (MQ=255)         ccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCAGTTAGCCGGGCAGCCCCGCCAGACATCGCTGAGTGTTGGGGCgggggtg                                                                                                                                               >  8:283590/1‑145 (MQ=255)          cagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑Gc                                                                                                                                             >  4:46993/1‑149 (MQ=255)            gacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑Gccc                                                                                                                                           <  2:52787/149‑1 (MQ=255)              cgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCcgc                                                                                                                                         <  3:46993/149‑1 (MQ=255)               gCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCAGTTAGCGGCCAAACCCGCCCGTTCATAGCTAAGTTTAGGAGCCGGCGT‑Gccccc                                                                                                                                         >  1:300949/1‑146 (MQ=255)                     gTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAgg                                                                                                                                  <  6:139399/149‑1 (MQ=255)                      ttCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTAGCGGGCAAGCCCGCCCGTACCTGGGGGAGGGTTGGAGCCGGCTT‑GCCCGCGAACCGGc                                                                                                                                 >  2:1398/1‑149 (MQ=255)                              ggcgCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTACGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTGCAGAGAGCTGTGTTGGGGCGGGGTT‑GGCCGCGGACCGGCCC‑GAGAAt                                                                                                                        >  7:317891/1‑149 (MQ=255)                               gcgcAGTACCGGGCCGGCGAGAACGAGGGCCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAAtt                                                                                                                       <  3:162633/149‑1 (MQ=255)                                 gcAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCGTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTc                                                                                                                      >  1:216490/1‑148 (MQ=255)                                   aGTACCGGGCCTGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCACTTACATCTATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAAt                                                                                                                   <  7:264070/149‑1 (MQ=255)                                    gTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGCGGGCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGAGCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATa                                                                                                                  <  3:228235/149‑1 (MQ=255)                                    gTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATa                                                                                                                  <  4:298843/149‑1 (MQ=255)                                       ccGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCAGTTAGCGGGCAAGCCCGCCCGTTAAAGGCTGACTGTTGGCTCGGGCTT‑TGCAGCGAACACGCCC‑GAGAATTaaaaaac                                                                                                                >  4:229554/1‑144 (MQ=255)                                          ggCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTAGCGGGCAACCCCCCCCATACAAAAAAAAGTGTTAGATCGGGGTT‑TACAGCCGACACGCCC‑GAGAATTCAATaaaaaa                                                                                                             >  4:144768/1‑143 (MQ=255)                                           gCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTAGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCGT‑TCCCGCGCACAGGCCC‑GAGAATTCAATACACAAc                                                                                                            >  6:138795/1‑148 (MQ=255)                                                cGAGAACGAGGGGCAACGGGTTAGGGTAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGc                                                                                                      <  3:272158/149‑1 (MQ=255)                                                   gaACGTGGGGCAACTGGTTAGGGGAGGTCATCGACAGCTCGGGGGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTg                                                                                                   <  5:16810/149‑1 (MQ=255)                                                   gaACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTg                                                                                                   >  1:85510/1‑149 (MQ=255)                                                     aCGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCCTGAGAATTCAATACACAACTAAGGCGTgg                                                                                                  <  1:205600/149‑1 (MQ=255)                                                        aGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGcaaa                                                                                              <  7:182391/149‑1 (MQ=255)                                                              aaCGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTAGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAAc                                                                                        >  1:217274/1‑149 (MQ=255)                                                                   gTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTAGCGGGCAAGCCCCCCCATACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GACAAATAAAAAAACAACTAAGGCGTGGCAAAAAAAACAGGCg                                                                                   >  4:301112/1‑149 (MQ=38)                                                                    ttAGGGGAGGTCATCGACAGCTCGGGTGGCTTTGGCGACCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCaa                                                                                                                    >  1:37368/1‑115 (MQ=255)                                                                    ttAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGACCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCaa                                                                                                                    <  2:37368/115‑1 (MQ=255)                                                                       ggggAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGaaa                                                                               <  3:301112/149‑1 (MQ=255)                                                                           aGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGc                                                                                    <  7:175165/140‑1 (MQ=255)                                                                           aGGTCATCGACAGCTCGGGTGGCTATGGCGGCCAGTTAGCGGGCAAGCCCCCCCGAACATCGATCAGTGTAGGAGCCGGCTT‑GCCCGCGGACAGGCCC‑GAGGAATCAAAACACAAAAAACGCGGGGGCAACAAAACCGGCGGTAAATaa                                                                           >  6:270422/1‑148 (MQ=255)                                                                                aTCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGaa                                                                       <  3:144768/148‑1 (MQ=255)                                                                                        cTCGGGTGGCTATGGCGGCCTGTTAGCGGGCAAGCCCGCCCATAAAAAGATAAGTGTAGGAGCGGGCTT‑GCCCCCGAACAGGGCC‑CAGAATTAAAAAAACAACAAATGCGTGGCAAACAAAACGGGCGGAAAATACTGAAAACTTcacg                                                              >  4:283180/1‑149 (MQ=255)                                                                                               ggCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCa                                                       <  1:1398/149‑1 (MQ=255)                                                                                               ggCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCa                                                       <  3:36658/149‑1 (MQ=255)                                                                                                           cTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGaa                                           <  7:283590/149‑1 (MQ=255)                                                                                                           cTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCAGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGaa                                           >  1:282456/1‑149 (MQ=255)                                                                                                              ttCGCGGGCAAGCCCGCGCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAGGGCGTGGCCAGCAAAACGGGCGGAAGATACTGAACACTTGCCGCCCGTCAGGCCAGCACGAGCTg                                        >  8:208332/1‑149 (MQ=38)                                                                                                              ttCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGc                                                                                                 >  2:80532/1‑92 (MQ=255)                                                                                                              ttCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGc                                                                                                 <  1:80532/92‑1 (MQ=255)                                                                                                                cgcgGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGt                                       <  1:106544/148‑1 (MQ=255)                                                                                                                cgcgGGCAACCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGGAAACACGAACTGt                                       >  2:32632/1‑148 (MQ=255)                                                                                                                 gcgGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTa                                      <  2:217274/148‑1 (MQ=255)                                                                                                                   gggCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGGCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTAt                                     >  1:68874/1‑148 (MQ=255)                                                                                                                     gCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAgg                                 >  1:97518/1‑149 (MQ=255)                                                                                                                          cccgctcCTACACTGATCGATGTAAGGGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCttt                            >  5:118268/1‑149 (MQ=37)                                                                                                                            cgccaTTACTTCGTTCAGTGTTGGTCGGGGCTT‑GCCCGCTAAATGGCCC‑TTTATTTAATTACACAATTAAGGGGTGGCAAAAAAACCGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTc                          <  2:97518/149‑1 (MQ=255)                                                                                                                              cccTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAg                        <  2:306018/149‑1 (MQ=255)                                                                                                                                cTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGGAAACACGAACTGTATCAGGCCTTTTCAGCt                      <  1:32632/149‑1 (MQ=255)                                                                                                                                   aCATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTAt                    >  8:314805/1‑148 (MQ=255)                                                                                                                                     aTCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACggg                                                                                     <  2:255476/81‑1 (MQ=255)                                                                                                                                     aTCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAAATCAATACACAACTAAGGCGTGGCAAACAAAACggg                                                                                     >  1:255476/1‑81 (MQ=255)                                                                                                                                      tCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAAc                >  7:217755/1‑149 (MQ=255)                                                                                                                                       cGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACt               >  8:325483/1‑149 (MQ=255)                                                                                                                                       cGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACt               >  1:144303/1‑149 (MQ=255)                                                                                                                                         aTCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCaaa                                                 >  7:216416/1‑113 (MQ=255)                                                                                                                                         aTCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCaaa                                                 <  8:216416/113‑1 (MQ=255)                                                                                                                                         aTCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAAc                <  1:99755/146‑1 (MQ=255)                                                                                                                                         aTCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAAc                >  2:99755/1‑146 (MQ=255)                                                                                                                                             gtgtAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCaa         <  7:314805/149‑1 (MQ=255)                                                                                                                                              tgtAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAAg        <  3:229554/149‑1 (MQ=255)                                                                                                                                                tAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAAGCt      >  6:364254/1‑149 (MQ=255)                                                                                                                                                  ggAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAAGCttt    <  2:85510/149‑1 (MQ=255)                                                                                                                                                    aGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAAGCTTTTc  >  6:321529/1‑149 (MQ=255)                                                                                                                                                    aGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAAGCTTTTc  <  5:270422/149‑1 (MQ=255)                                                                                                                                                    aGCGGGCTT‑ACCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAAGCTTTTc  <  5:138795/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                       CCAGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTT‑GCCCGCGAACAGGCCC‑GAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAAGCTTTTC  >  NC_002947/2474015‑2474310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑