Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,509,062	G→C	8.4%	A18G (GCC→GGC)	PP_2203 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,509,062	0	G	C	8.4%	257.1 / 12.8	83	A18G (GCC→GGC)	PP_2203	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (37/39);  new base C (4/3);  total (41/42)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01

GCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCAGATCTTCGCCGTCTTCTCGGGCCTGGTTGGCACGC  >  NC_002947/2508915‑2509207                                                                                                                                                     |                                                                                                                                                  gcCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGc                                                                                                                                                  <  7:291231/149‑1 (MQ=255)    aCGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAg                                                                                                                                               <  6:284159/149‑1 (MQ=255)     cGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGTCTGGGCCAgg                                                                                                                                              <  3:298314/149‑1 (MQ=255)          aGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGGCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGCCCAGGCCgcc                                                                                                                                         <  6:84946/149‑3 (MQ=255)          aGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATAGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGCCCAGGCCgcc                                                                                                                                         <  2:215125/149‑3 (MQ=255)              gTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACg                                                                                                                                     >  7:252445/1‑149 (MQ=255)                   ttGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAgg                                                                                                                                              <  8:275873/135‑1 (MQ=255)                   ttGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAgg                                                                                                                                              >  7:275873/1‑135 (MQ=255)                     gACGGCGCTCATGATTGGCTCGCCGTGGGCGGTCACGGCAAAACCCTTCCCACGCAGGTGGCTGATCCCGGCCTTGCAGCAGCCGCAGTTGCCGTCGCGGTCCACGTCGATCGCCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCcag                                                                                                                                <  8:132541/147‑1 (MQ=255)                      aCGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCcagca                                                                                                                              <  4:227002/148‑1 (MQ=255)                         gcgcTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCt                                                                                                                          <  2:13635/149‑1 (MQ=255)                         gcgcTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCt                                                                                                                          <  7:29989/149‑1 (MQ=255)                           gcTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGATGACGGCCAGCAGGCt                                                                                                                          >  3:438/1‑147 (MQ=255)                            cTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAg                                                                                                                       <  7:162123/149‑1 (MQ=255)                            cTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAg                                                                                                                       <  7:46314/149‑1 (MQ=255)                            cTCATGTTTGGCTCGACGTGGGCGTGGACGGTAAACCCATTGTCACGCCGGTGGCTGTTCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAg                                                                                                                       <  4:76748/149‑1 (MQ=255)                             tCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAgg                                                                                                                      >  3:179292/1‑149 (MQ=255)                               aTGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGt                                                                                                                    >  1:168366/1‑149 (MQ=255)                               aTGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGAGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGt                                                                                                                    >  5:16172/1‑149 (MQ=255)                                 gTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGt                                                                                                                    >  4:171312/1‑147 (MQ=255)                                   ttGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGGCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTcag                                                                                                                 <  8:83696/148‑1 (MQ=255)                                     ggCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTcagca                                                                                                               <  3:171312/148‑1 (MQ=255)                                       cTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGgt                                                                                                            <  8:252445/149‑1 (MQ=255)                                           aCGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTAAGGGTCAGCAGGTGttt                                                                                                        >  5:262283/1‑149 (MQ=255)                                                 tCGTGGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCgcag                                                                                                  <  3:267252/149‑1 (MQ=255)                                                   gTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCgcagca                                                                                                >  5:1029/1‑149 (MQ=255)                                                   gTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCgca                                                                                                   <  8:122129/146‑1 (MQ=255)                                                   gTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCgca                                                                                                   >  7:122129/1‑146 (MQ=255)                                                    ttGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAgg                                                                                                                            <  6:715/120‑1 (MQ=255)                                                    ttGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAgg                                                                                                                            >  5:715/1‑120 (MQ=255)                                                       aCGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGt                                                                                            >  4:200873/1‑149 (MQ=255)                                                       aCGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGt                                                                                            <  3:99578/149‑1 (MQ=255)                                                          gTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTg                                                                                           >  3:207299/1‑147 (MQ=255)                                                           tAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGgt                                                                                                            >  4:5970/1‑129 (MQ=255)                                                           tAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGgt                                                                                                            <  3:5970/129‑1 (MQ=255)                                                             aaaCCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCa                                                                                      >  7:53247/1‑149 (MQ=255)                                                              aaCCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGa                                                                                          <  7:328984/144‑1 (MQ=255)                                                              aaCCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGa                                                                                          >  8:328984/1‑144 (MQ=255)                                                               aCCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACt                                                                                    >  1:76839/1‑149 (MQ=255)                                                                  aTTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCTGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACCGCCAGCAGGCTCACGGTCAGCAGGTGATTGCGCAGCATGGTGAAGCACTCCt                                                                                 >  7:679/1‑149 (MQ=255)                                                                        aCGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTaaa                                                                            >  5:326442/1‑148 (MQ=255)                                                                            aGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGc                                                                       <  5:227356/149‑1 (MQ=255)                                                                              gTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAg                                                                     >  7:84150/1‑149 (MQ=255)                                                                              gTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAg                                                                     >  3:54428/1‑149 (MQ=255)                                                                               tGGGTGATCCCGGCCTTGCAGCTGCCGCAGTTGGGGTCTCGGTAGACGGCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAgg                                                                    <  8:679/149‑1 (MQ=255)                                                                                   tGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGa                                                                >  1:43081/1‑149 (MQ=255)                                                                                      tCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATAc                                                             <  8:84150/149‑1 (MQ=255)                                                                                       ccAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGCGGGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATAc                                                             >  1:280607/1‑149 (MQ=255)                                                                                       ccAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATAcc                                                            >  5:103919/1‑149 (MQ=255)                                                                                        cAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCg                                                           <  6:16172/149‑1 (MQ=255)                                                                                             cTTGCAGCAGCCGCTGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGAc                                                      <  2:179607/149‑1 (MQ=255)                                                                                              ttGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGgtgt                                                                                                          >  8:263643/1‑96 (MQ=255)                                                                                              ttGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGgtgt                                                                                                          <  7:263643/96‑1 (MQ=255)                                                                                                gcagcaGCCTCAGTTGGGGTCGCGGTAGACGTAGATGGTCTGGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGGTTGCGCAGGATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGAcc                                                     <  3:324367/147‑1 (MQ=255)                                                                                                 cagcagCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAgg                                                  >  3:193580/1‑149 (MQ=255)                                                                                                    cagcCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCgg                                               <  3:200873/149‑1 (MQ=255)                                                                                                         gCAGTTGGGGTCGCGGTAGACGTCGATGGGCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGGGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTg                                          <  8:205890/149‑1 (MQ=255)                                                                                                            gTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGc                                                                   <  7:45269/121‑1 (MQ=255)                                                                                                            gTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGc                                                                   >  8:45269/1‑121 (MQ=255)                                                                                                            gTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGcc                                       <  6:1029/149‑1 (MQ=255)                                                                                                               ggggTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCa                                     <  2:280607/148‑1 (MQ=255)                                                                                                                 ggTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGcc                                       >  3:250036/1‑144 (MQ=255)                                                                                                                  gTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCAGATc                                 >  2:215554/1‑149 (MQ=255)                                                                                                                  gTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCAGATc                                 >  4:229285/1‑149 (MQ=255)                                                                                                                     gcgGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCAGATCTTc                              <  6:103919/149‑1 (MQ=255)                                                                                                                       ggTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGGCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAATAGGCTGGCGGATTCCGGCGACCAGGCGGGTCTGGCCCAGATGTTCGc                            <  6:326442/149‑1 (MQ=255)                                                                                                                             cGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCAGATCTTCGCCGtctt                      <  1:215554/149‑1 (MQ=255)                                                                                                                              gTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGc                                        >  7:337685/1‑130 (MQ=255)                                                                                                                              gTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGc                                        <  8:337685/130‑1 (MQ=255)                                                                                                                                 gATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCAGATCTTCGCCGTCTtctc                   <  8:53247/148‑1 (MQ=255)                                                                                                                                  aTGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCAGATCTTCGCCGTCTTCTCgg                 >  3:8544/1‑149 (MQ=255)                                                                                                                                  aTGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCAGATCTTCGCCGTCTTCTCgg                 <  4:179292/149‑1 (MQ=255)                                                                                                                                  aTGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCAGATCTTCGCCGTCTTCTCgg                 <  4:54428/149‑1 (MQ=255)                                                                                                                                     gTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCAGATCTTCGCCGTCTTCTCGGGcc              >  7:244743/1‑149 (MQ=255)                                                                                                                                       ctcGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCAGATCTTCGCCGTCTTCTCGGGCCTg            >  6:225101/1‑149 (MQ=255)                                                                                                                                        tcacc‑ggcCTGGCCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGccc                                      >  7:642/5‑122 (MQ=255)                                                                                                                                        tcacc‑ggcCTGGCCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGccc                                      <  8:642/118‑1 (MQ=255)                                                                                                                                        tcacc‑ggcCTGGCCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCAGATCTTCGCCGTCTTCTCGGGCCTgg           >  7:49466/5‑149 (MQ=255)                                                                                                                                         cacc‑ggcCTGGCCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCAGATCTTCGCCGTCTTCTCGGGCCTGGt          >  8:96113/4‑149 (MQ=255)                                                                                                                                           gc‑ggcCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCAGATCTTCGCCGTCTTCTCGGGCCTGGTTg        >  7:191437/1‑149 (MQ=255)                                                                                                                                            c‑ggcCTGGCCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCAGATCTTCGCCGTCTTCTCGGGCCTGGTTgg       >  3:275167/1‑149 (MQ=255)                                                                                                                                               gcCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGGCCGGATCTTCGCCGTCGTCTCGGGCCTGGTTGGCa     >  6:239225/1‑149 (MQ=255)                                                                                                                                                 cTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCAGATCTTCGCCGTCTTCTCGGGCCTGGTTGGCAcg   >  7:81962/1‑149 (MQ=255)                                                                                                                                                    ggCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCAGATCTTCGCCGTCTTCTCGGGCCTGGTTGGCAcgc  <  6:262283/147‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGC‑GGCCTGGGCCAGGCCGGTGACGGCCAGCAGGCTCAGGGTCAGCAGGTGTTTGCGCAGCATGGTGAAGCACTCCTTTAAAAACGCAGGCGGATACCGGCGACCAGGCGGGTCTGGCCCAGATCTTCGCCGTCTTCTCGGGCCTGGTTGGCACGC  >  NC_002947/2508915‑2509207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑