Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,571,850 A→T 8.0% L196Q (CTG→CAG)  dctA‑III ← C4‑dicarboxylate transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,571,8500AT 8.0% 141.3 / 2.6 50L196Q (CTG→CAG) dctA‑IIIC4‑dicarboxylate transport protein
Reads supporting (aligned to +/- strand):  ref base A (23/23);  new base T (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

GCGCATGACGGTGCCCAGCACGATCAGCACGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTGAAGAATGTTGTTGTCGG  >  NC_002947/2571705‑2571997
                                                                                                                                                 |                                                                                                                                                   
gcgcATGACGGTGCCCAGCACGATCAGCACGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGgc                                                                                                                                                  <  7:247862/149‑1 (MQ=255)
gcgcATGACGGTGCCCAGCACGATCAGCACGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGgc                                                                                                                                                  >  5:210252/1‑149 (MQ=255)
       aCGGTGCCCAGCACGATCAGCACGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGAt                                                                                                                                           >  3:281784/1‑149 (MQ=255)
            gCCCGGCACGATCAGCACGAAGCCGCCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGc                                                                                                                                      >  7:82622/1‑149 (MQ=255)
             cccAGCACGATCAGCACGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGc                                                                                                                                      <  3:357746/148‑1 (MQ=255)
              ccAGCACGATCAGCACGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGCCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGcc                                                                                                                                     >  5:84178/1‑148 (MQ=255)
                  cACGATCAGCACGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCatga                                                                                                                                <  3:198801/149‑1 (MQ=255)
                   aCGATCAGCACGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCGGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCatgat                                                                                                                               >  3:312197/1‑149 (MQ=255)
                         aGCACGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCACCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGaa                                                                                                                         <  6:347840/149‑1 (MQ=255)
                            aCGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGAt                                                                                                                      <  1:175957/149‑1 (MQ=255)
                              gAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATca                                                                                                                    >  6:324088/1‑149 (MQ=255)
                                   cGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATgg                                                                                                               <  2:43619/149‑1 (MQ=255)
                                            tGAGGTAGAATAGCGCCACCAGCCCGCCGAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGAGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCg                                                                                                      <  4:215884/149‑1 (MQ=255)
                                              aGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACTATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGtt                                                                                                    <  6:210252/149‑1 (MQ=255)
                                                   gAACAGCGGCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATc                                                                                                <  1:242148/148‑1 (MQ=255)
                                                      cAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTc                                                                                            >  8:82361/1‑149 (MQ=255)
                                                      cAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTc                                                                                            >  8:77401/1‑149 (MQ=255)
                                                             accaGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAAt                                                                                     >  1:95033/1‑149 (MQ=255)
                                                               caGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGc                                                                                   <  4:281784/149‑1 (MQ=255)
                                                                  cccgcccAGGTGCTGCTGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCcgg                                                                                <  4:312197/149‑1 (MQ=255)
                                                                  cccgcccAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATAAGTCGCGAAATGCcgg                                                                                >  3:233170/1‑149 (MQ=255)
                                                                   ccgcccAGGTGCAGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGTGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTACGTTGATCAGTCGCGAAATGCcggc                                                                               >  4:306245/1‑149 (MQ=255)
                                                                     gcccAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCcggcgg                                                                             <  7:119029/149‑1 (MQ=255)
                                                                        cAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCg                                                                          <  3:323203/149‑1 (MQ=255)
                                                                         aGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGa                                                                         <  7:82361/149‑1 (MQ=255)
                                                                         aGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGa                                                                         <  7:77401/149‑1 (MQ=255)
                                                                                gcAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTcacc                                                                   >  2:290446/1‑148 (MQ=255)
                                                                                   gCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTAGCGAAATGCCGGCGGCCGAGTcaacaa                                                                 >  3:224906/1‑143 (MQ=255)
                                                                                    cGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACccacca                                                              <  5:324088/149‑1 (MQ=255)
                                                                                                    ttGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGcc                                              >  2:279873/1‑149 (MQ=255)
                                                                                                          gtggtgAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAAc                                          <  4:233170/147‑1 (MQ=255)
                                                                                                          gtggtgAAGGCGATGCCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACa                                         <  6:84178/148‑1 (MQ=255)
                                                                                                                aaGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCATGGCGCTGCCGAACAGCAcc                                    >  8:288761/1‑147 (MQ=255)
                                                                                                                      aTGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAAcag                            <  1:290446/149‑1 (MQ=255)
                                                                                                                      aTGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAAcag                            >  1:308362/1‑149 (MQ=255)
                                                                                                                           aCCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAAc                                          <  6:54944/130‑1 (MQ=255)
                                                                                                                           aCCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAAc                                          >  5:54944/1‑130 (MQ=255)
                                                                                                                           aCCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAgcagca                       >  4:282595/1‑149 (MQ=255)
                                                                                                                                 cacGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTg                   >  8:10411/1‑147 (MQ=255)
                                                                                                                                    gCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCTAACAGCACCGAGAACAGCAGCACCTgaagaa              >  6:51145/1‑149 (MQ=255)
                                                                                                                                    gCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAAca                             >  6:305330/1‑134 (MQ=255)
                                                                                                                                    gCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAAca                             <  5:305330/134‑1 (MQ=255)
                                                                                                                                      gatcgtgcgcCTGGCGC‑CGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTGAAGAAtgt           >  3:262703/7‑149 (MQ=255)
                                                                                                                                      gatcgtgcgcCTGGCGC‑CGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTGAAGAAtg            <  4:262703/142‑1 (MQ=255)
                                                                                                                                       atcgtgcgcCTGGCGC‑CGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTgaa                 <  4:320648/137‑1 (MQ=255)
                                                                                                                                       atcgtgcgcCTGGCGC‑CGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTgaa                 >  3:320648/6‑142 (MQ=255)
                                                                                                                                           ggCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTGAATAAtgttgtt       <  3:282595/149‑1 (MQ=255)
                                                                                                                                            gcgcCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTGAAGAAtgttgtt       <  3:104992/148‑1 (MQ=255)
                                                                                                                                            gcgcCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTGAAGAAtgttgtt       >  4:104992/1‑148 (MQ=255)
                                                                                                                                                cAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGGACCGAGAACAGCAGCACCTGAAGAATGTTGTTGTCgg  >  2:76396/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCGCATGACGGTGCCCAGCACGATCAGCACGAAGCCGGCACAGGTGAGGTAGAACAGCGCCACCAGCCCGCCCAGGTGCTGCAGCGACGCCAGGCCGTACTTGCTGGTGGTGAAGGCGATGGCACCGAACACGCCGATCGGCGCCAGGCGCACGATCATGCCCATGATGCGGAAGATCACATGGCTCAGTTCGTTGATCAGTCGCGAAATGCCGGCGGCCGAGTCACCCACCAGGTTCAGGGCGCTGCCGAACAGCACCGAGAACAGCAGCACCTGAAGAATGTTGTTGTCGG  >  NC_002947/2571705‑2571997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑