Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	27,581	C→T	12.3%	intergenic (‑428/+18)	PP_0022 ← / ← PP_0023	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	27,581	0	C	T	12.3%	161.5 / 12.5	65	intergenic (‑428/+18)	PP_0022/PP_0023	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (25/32);  new base T (2/6);  total (27/38)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.47e-01

CAGTCTCGTCGTCCGCACTGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGATGCTCATG  >  NC_002947/27435‑27728                                                                                                                                                     |                                                                                                                                                    cAGTCTCGTCGTCCGCACTGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTccc                                                                                                                                                    >  5:221445/1‑148 (MQ=255)  aGTCTCGTCGTCCGCACTGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCga                                                                                                                                                  <  4:178299/149‑1 (MQ=255)      tcgtcgTCCGCACTGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCACTCGGGAgc                                                                                                                                              >  7:289099/1‑149 (MQ=255)              cGCACTGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCTCGGGAGCTGCttttt                                                                                                                                      <  6:294394/149‑1 (MQ=255)              cGCACTGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCttttt                                                                                                                                      <  6:35542/149‑1 (MQ=255)               gCACTGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCTCGGGAGCTGCtttttt                                                                                                                                     <  7:11476/149‑1 (MQ=255)                cACTGAAGGGATAACCGATCCTGAGTTAGCTTAAGAATCCATCAAGCAAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCTCGGGAGCTGCttttttt                                                                                                                                    <  8:289099/149‑2 (MQ=255)                  cTGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCTCGGGAGCTGCTtttttttt                                                                                                                                  <  2:290490/149‑1 (MQ=255)                  cTGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGGCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCTCGGGAGCTGCTtttttttt                                                                                                                                  <  2:229892/149‑1 (MQ=255)                   tGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACTCGGTCCGATTGATCAGCGAGACGGTCGTGAGCGTTTGCACACAGACCC‑AAAAA‑AAAAGCAGCTACCGAGAGCTGCTTTTTTCtt                                                                                                                                  >  7:234749/1‑148 (MQ=255)                   tGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTc                                                                                                                                    >  1:263644/1‑146 (MQ=255)                   tGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTc                                                                                                                                    <  2:263644/146‑1 (MQ=255)                    gAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGAACCCCGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGCGCTGCTTTTTTCTTAc                                                                                                                                >  4:9682/1‑149 (MQ=255)                     aatggATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGTAAGACTCACGACGCTGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTAc                                                                                                                                <  8:109/145‑1 (MQ=255)                     aaGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACt                                                                                                                               >  1:274696/1‑149 (MQ=255)                     aaGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGGTTGCACACAGCCCC‑AAAAA‑AAAAGCACCTCCCGAGAGCTGCTTTTTTCTTa                                                                                                                                 <  5:254404/147‑1 (MQ=255)                       gggATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTcc                                                                                                                             >  8:204833/1‑149 (MQ=255)                              ccGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCtttt                                                                                                                      >  3:263362/1‑149 (MQ=255)                                  tCCGGAGGTAGCTTCAGAATCCATCAATCAAAGCGGAAGACTCACGACGCGGTCCGAATGATCAGCGAGACGGTCGTGAGGGATTGCACACAGCCCC‑AAAAA‑AAAACCAGATCCCCACACCTGCTTATTAATTACACACCCGTTTagt                                                                                                                   >  8:37737/1‑148 (MQ=255)                                     ggAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCtt                                                                                                               >  1:136151/1‑149 (MQ=255)                                             cTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGtt                                                                                                       >  8:7421/1‑149 (MQ=255)                                              ttCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTAATCCCCCTTTTTGTGCTTGTGGGGtt                                                                                                       >  8:161116/1‑148 (MQ=255)                                               tCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGACACGGTCGTGAGGGTATGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCAGCTTTTTTCTTACTCCCCCTATTTTTGCTTGTGGGGttt                                                                                                      >  4:85490/1‑148 (MQ=255)                                                cAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGttt                                                                                                      >  3:265716/1‑147 (MQ=255)                                                cAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTc                                                                                                     <  4:263362/148‑1 (MQ=255)                                                  gAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAgg                                                                                                  >  5:265652/1‑149 (MQ=255)                                                   aaTCCATCAAGCCAAGGGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGt                                                                                                 <  7:38346/149‑1 (MQ=255)                                                    aTCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGt                                                                                                 >  8:38346/1‑148 (MQ=255)                                                     tCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTcc                                                                                               <  6:265652/149‑1 (MQ=255)                                                       cATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCgg                                                                                             >  7:277239/1‑149 (MQ=255)                                                           aagccaagcGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATg                                                                                          <  7:204833/148‑1 (MQ=255)                                                               caagcGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTgg                                                                                                           >  4:172534/1‑127 (MQ=255)                                                               caagcGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTgg                                                                                                           <  3:172534/127‑1 (MQ=255)                                                                 agcGGAAGACTCACGACGCGGTCCGTTTGATCAGCGAGACGGTCGTGTGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTc                                                                                   <  3:85490/149‑1 (MQ=255)                                                                           tCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCCAAAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCtttt                                                                            >  1:102374/1‑147 (MQ=255)                                                                             acgccgCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAgg                                                                        <  8:234749/148‑1 (MQ=255)                                                                             acgacgCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAggg                                                                       >  5:6370/1‑149 (MQ=255)                                                                             acgacgCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAggg                                                                       >  1:135650/1‑149 (MQ=255)                                                                             acgacgCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAggg                                                                       <  2:274696/149‑1 (MQ=255)                                                                             acgacgCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAggg                                                                       <  4:265716/149‑1 (MQ=255)                                                                               gacgCGGTCCTTTTTATCTTCGAGCCGGTCGTGCTGGTTTGCTCACAGGCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCGCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGt                                                                     <  2:79214/149‑1 (MQ=255)                                                                               gacgCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGt                                                                     <  6:6370/149‑1 (MQ=255)                                                                                     gTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACCCACCCCC‑AAAAACAACAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTc                                                                <  4:199417/149‑1 (MQ=255)                                                                                       ccGATTCTTCAGCCTGTCGGTCGTGAGTGTTTGCACACAGCCCC‑AAAAT‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCt                                                             <  7:37737/149‑1 (MQ=255)                                                                                        cGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTg                                                            >  2:219640/1‑149 (MQ=255)                                                                                         gATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGc                                                           <  6:221445/149‑1 (MQ=255)                                                                                               tCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTATTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGTTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCTCTGCATTTTg                                                     >  7:165990/1‑149 (MQ=255)                                                                                                 aGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTc                                                   <  2:136151/149‑1 (MQ=255)                                                                                                   cGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCg                                                 <  1:219640/149‑1 (MQ=255)                                                                                                             gTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTc                                                                                                >  6:50449/1‑92 (MQ=255)                                                                                                             gTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTc                                                                                                <  5:50449/92‑1 (MQ=255)                                                                                                                           acaGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTATGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCt                         >  3:303411/1‑149 (MQ=255)                                                                                                                              gCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTg                      <  7:161116/149‑1 (MQ=255)                                                                                                                               cccc‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGAGGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTgg                     >  3:249594/1‑149 (MQ=255)                                                                                                                                gagtaagaa‑aaaaGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTgg                     <  1:164387/142‑1 (MQ=255)                                                                                                                                       ta‑aaaaGCAGCTCCCGCGAGGTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGc              <  8:165990/148‑1 (MQ=255)                                                                                                                                       aa‑aaaaGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCa                                         >  6:174868/1‑122 (MQ=255)                                                                                                                                       aa‑aaaaGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCa                                         <  5:174868/122‑1 (MQ=255)                                                                                                                                          aaaaGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCtt            <  2:102374/149‑1 (MQ=255)                                                                                                                                           aaaGCAGCTCTCGGGAGCTGCTTTTTTTTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGt                                                                     <  8:235974/91‑1 (MQ=255)                                                                                                                                           aaaGCAGCTCTCGGGAGCTGCTTTTTTTTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTACGGATGGTCCTTCTGCTTTTCAGGGGt                                                                     >  7:235974/1‑91 (MQ=38)                                                                                                                                            aaGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGa          <  7:7421/149‑1 (MQ=255)                                                                                                                                            aaGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGa          <  2:135650/149‑1 (MQ=255)                                                                                                                                            aaGCAGATCCCGATAGCTGCTTTTTTCTTACTCCCCCGAGTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGa          <  4:68807/149‑1 (MQ=255)                                                                                                                                               cagcTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGATGc       <  3:9682/149‑1 (MQ=255)                                                                                                                                                  ctctCGGGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCa                                                                          <  7:69340/79‑1 (MQ=255)                                                                                                                                                  ctctCGGGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCa                                                                          >  8:69340/1‑79 (MQ=255)                                                                                                                                                  cTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGATGCTCa    <  8:277239/149‑1 (MQ=255)                                                                                                                                                     ccGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGATGCTCATg  >  2:4417/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CAGTCTCGTCGTCCGCACTGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAA‑AAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGATGCTCATG  >  NC_002947/27435‑27728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑